BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0513.0006.p (219 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE0797 [IQR] COG1028 Dehydrogenases with different specificitie... 67 2e-11 PA0117 [IQR] COG1028 Dehydrogenases with different specificities... 66 5e-11 L118271 [IQR] COG1028 Dehydrogenases with different specificitie... 63 4e-10 AGl1487 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 63 4e-10 MA0107 [IQR] COG1028 Dehydrogenases with different specificities... 62 6e-10 >PAE0797 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 67.0 bits (162), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 40/158 (25%), Positives = 78/158 (49%), Gaps = 6/158 (3%) Query: 6 IVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELE-QLGIEAAPFQADILNKEQ 64 + A G+G +A + G + + +RN E+L L +L Q G+ P QAD+ +E Sbjct: 10 VTAASRGIGAGVATVLAREGSNLVIAARNLERLEGLAKQLSSQYGVSVVPVQADLTRRED 69 Query: 65 IEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQVLP 124 + +G +D+L Y+ P L++TE++ Y + ++ A+ + VLP Sbjct: 70 VRKIVDEALRHFGKVDILAYNTGPP--KPGTFLELTEDDWDYGVRLLLMSAVWITKDVLP 127 Query: 125 EMLDKQSGALLFTTGGAAIHPVPMM---GNVGIAISGL 159 M++++ G L++ T P+P + + I+++GL Sbjct: 128 YMIERRWGRLIYITSSTLKQPIPTLTLSNVIRISLAGL 165 >PA0117 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 242 Score = 65.9 bits (159), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 45/178 (25%), Positives = 79/178 (44%), Gaps = 2/178 (1%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 K + ++GAG G +IA++F + G+ + RN EKL LV + G EA D Sbjct: 5 KAVLVMGAGDATGGAIARRFAREGYVACVARRNAEKLEPLVQAIRDQGGEALACGCDARQ 64 Query: 62 KEQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQ 121 ++Q+ FA I+ + G ++ + ++ N P + TE ++ G + R+ Sbjct: 65 EQQVIDLFARIEGEVGALEAVIFN--VGANVWFPITETTERVYRKVWEMAAFGGFLTGRE 122 Query: 122 VLPEMLDKQSGALLFTTGGAAIHPVPMMGNVGIAISGLRNYIFNLNSELADKGVYAGH 179 ML +Q G ++FT A++ A LR ++ EL K ++ H Sbjct: 123 AARVMLPRQRGTIIFTGATASLRGRAHFAAFSGAKFALRALAQSMARELGPKDIHVAH 180 >L118271 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 253 Score = 62.8 bits (151), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 35/98 (35%), Positives = 59/98 (60%), Gaps = 7/98 (7%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 K A+ GAG G+G +IAK+ +GF+VA+I NEE + ++LG + +AD+ + Sbjct: 3 KIAAVTGAGQGIGFAIAKRLYNDGFKVAIIDYNEETAQKAA---KELGENSFAIKADVSD 59 Query: 62 KEQIEVAFAAIKEKYGFIDVL----EYSPTPSINTVTP 95 +EQ+ A A+ +K+G ++V+ +PT I T+TP Sbjct: 60 REQVISALNAVVDKFGDLNVVVNNAGIAPTTQIETITP 97 >AGl1487 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Length = 134 Score = 62.8 bits (151), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 0/174 (0%), Positives = 0/174 (0%), Gaps = 7/174 (4%) Query: 3 TIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILNK 62 TIA++G + A +N + + A + Sbjct: 4 TIAVLGLITFSSPAFAASCEKNFTVSGVPMVTAVSYKSFQELPKA-KAPAVLQ--KLAQA 60 Query: 63 EQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQV 122 E K + S V + V + Sbjct: 61 VAAEGFSGIQINKALSSIDAHQETSGSGRIQ-TLRVVARQKGAAVRIDAVFNIQAGQIAD 119 Query: 123 LPEMLDKQSGALLFTTGGAAIHPVPMMGNV-GIAISGLRNYIFNLNSELADKGV 175 + Sbjct: 120 KDVIR-- 171 >MA0107 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 62.0 bits (149), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 47/171 (27%), Positives = 78/171 (45%), Gaps = 6/171 (3%) Query: 2 KTIAIVGAGAGLGMSIAKKFGQNGFRVALISRNEEKLNQLVIELEQLGIEAAPFQADILN 61 K + GA +GLG+ A+ G +A+I+R EEKL ++ E+E+LG+ + D++ Sbjct: 10 KVAIVTGASSGLGVEFARALANQGANIAIIARREEKLKEVQKEIEKLGVTCRYYLCDVMK 69 Query: 62 KEQIEVAFAAIKEKYGFIDVLEYSPTPSINTVTPTLDVTEENALYQFQFNVLGALSSVRQ 121 EQI+ A + + +G ID+L + V T+E N+ G R+ Sbjct: 70 TEQIKNAVEQVVKDFGRIDIL--VNNAGLGLVDAADKTTDEMWHNMIDTNLNGVYFFARE 127 Query: 122 VLPEMLDKQSGALLFTTGGAAIHP-VPMMGNVGIAISGLRNYIFNLNSELA 171 V ML ++ G ++ +IH V M G A + + L LA Sbjct: 128 VGKIMLRQKHGRIINI---GSIHSTVSMKGLPVTAYCSTKGGVLMLTKSLA 175 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,438,866 Number of extensions: 650604 Number of successful extensions: 2179 Number of sequences better than 1.0e-05: 79 Number of HSP's gapped: 2236 Number of HSP's successfully gapped: 79 Length of query: 219 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 117 Effective length of database: 26,614,363 Effective search space: 3113880471 Effective search space used: 3113880471 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)