BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0513.0003.p (210 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0538 [O] COG0826 Collagenase and related proteases 198 5e-51 CAC2341 [O] COG0826 Collagenase and related proteases 184 1e-46 MTH1763 [O] COG0826 Collagenase and related proteases 176 3e-44 FN1931 [O] COG0826 Collagenase and related proteases 174 1e-43 FN1826 [O] COG0826 Collagenase and related proteases 160 1e-39 >MA0538 [O] COG0826 Collagenase and related proteases Length = 855 Score = 198 bits (504), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 99/199 (49%), Positives = 123/199 (61%) Query: 2 TELLAPAGNMEALKAAISNGCDAVYLGMQKFGARAYSSNFDVESLQEAVTYAHLRDVKIY 61 ELLAPAG MEALKAA+ NG DAVY+G + F AR Y+SNF E L++A+ YAHLR VKIY Sbjct: 6 PELLAPAGGMEALKAAVENGADAVYIGARAFSARGYASNFSEEELEQAIDYAHLRGVKIY 65 Query: 62 VTMNTIVFENEVEEMKKQIRELNEIGVDGIIVQDLTAFDYIVNNFLDMEAHCSTQMGIDD 121 VT+NT++ E EVE + L E G D II+QD D+ H STQM + + Sbjct: 66 VTVNTLLKEGEVESALILLSRLRETGADAIIIQDPGLIALSRKYLPDLPLHASTQMTLHN 125 Query: 122 LDGTLLFKKLGAKRVVLSXXXXXXXXXXXXXXADIPLEIFVHGALCVSYSGNCLMSGLTG 181 +G K LG +RVVLS +D+ +E+FVHGALC+SYSG CL+S L G Sbjct: 126 SEGVAFAKDLGIERVVLSRETSLEEIRKIKEKSDMEIEVFVHGALCISYSGQCLLSSLIG 185 Query: 182 FRCANRGRCVGSCRKEYEL 200 R NRG C CRK+Y L Sbjct: 186 GRSGNRGYCAQPCRKKYRL 204 >CAC2341 [O] COG0826 Collagenase and related proteases Length = 787 Score = 184 bits (466), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 92/198 (46%), Positives = 126/198 (63%) Query: 3 ELLAPAGNMEALKAAISNGCDAVYLGMQKFGARAYSSNFDVESLQEAVTYAHLRDVKIYV 62 ELLAPAG++E++ AA+ NG DAVY+G +F ARAY+ NF+ E +++AV Y H V++YV Sbjct: 5 ELLAPAGSIESIYAAVQNGADAVYVGGSRFSARAYAENFNDEIMEKAVDYCHDYGVRLYV 64 Query: 63 TMNTIVFENEVEEMKKQIRELNEIGVDGIIVQDLTAFDYIVNNFLDMEAHCSTQMGIDDL 122 T+NT++ ++E++E+ + I L +IGVD +IVQD I D E H STQM I + Sbjct: 65 TVNTLIKQDEIKEVIRYIEFLYKIGVDALIVQDTAILQLIKRYVPDFEIHASTQMTIHNA 124 Query: 123 DGTLLFKKLGAKRVVLSXXXXXXXXXXXXXXADIPLEIFVHGALCVSYSGNCLMSGLTGF 182 G + +K+ G KR+VLS +I E+FVHGALCV YSG CLMS + G Sbjct: 125 LGAVYYKERGFKRIVLSRELSLREIEYISKELNIETEVFVHGALCVCYSGQCLMSSIIGG 184 Query: 183 RCANRGRCVGSCRKEYEL 200 R NRGRC CR+ Y L Sbjct: 185 RSGNRGRCAQPCRQSYTL 202 >MTH1763 [O] COG0826 Collagenase and related proteases Length = 268 Score = 176 bits (445), Expect = 3e-44, Method: Composition-based stats. Identities = 11/200 (5%), Positives = 40/200 (20%) Query: 1 MTELLAPAGNMEALKAAISNGCDAVYLGMQKFGARAYSSNFDVESLQEAVTYAHLRDVKI 60 +L + + + + + + + +RD+ Sbjct: 3 RVDLSIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILT 62 Query: 61 YVTMNTIVFENEVEEMKKQIRELNEIGVDGIIVQDLTAFDYIVNNFLDMEAHCSTQMGID 120 Y+ + M++ + L ++ + Sbjct: 63 YMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDI 122 Query: 121 DLDGTLLFKKLGAKRVVLSXXXXXXXXXXXXXXADIPLEIFVHGALCVSYSGNCLMSGLT 180 D L ++ ++S + + + L + + + Sbjct: 123 GADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVG 182 Query: 181 GFRCANRGRCVGSCRKEYEL 200 R + Sbjct: 183 ADVVVATSRNASPITSILDE 202 >FN1931 [O] COG0826 Collagenase and related proteases Length = 720 Score = 174 bits (440), Expect = 1e-43, Method: Composition-based stats. Identities = 86/197 (43%), Positives = 122/197 (61%) Query: 3 ELLAPAGNMEALKAAISNGCDAVYLGMQKFGARAYSSNFDVESLQEAVTYAHLRDVKIYV 62 +++APAGNME +AIS D +YLG++ FGAR + NF VE L++A+ YAHLR +I++ Sbjct: 2 KIVAPAGNMERFYSAISATADEIYLGLKGFGARRNAENFTVEELKKAIDYAHLRGSRIFL 61 Query: 63 TMNTIVFENEVEEMKKQIRELNEIGVDGIIVQDLTAFDYIVNNFLDMEAHCSTQMGIDDL 122 T+NTI+ E+E + +++L G+D IIVQD+ +Y+ NF +E H STQM + + Sbjct: 62 TLNTIMTNREIELLYPTLKDLYNYGLDAIIVQDIGYAEYLHKNFPSIEIHGSTQMTVANY 121 Query: 123 DGTLLFKKLGAKRVVLSXXXXXXXXXXXXXXADIPLEIFVHGALCVSYSGNCLMSGLTGF 182 K+LG KR+VL D+ LE+FV G+LC+S+SGNC MS G Sbjct: 122 YEINYLKELGFKRIVLPRELSFEEIKEIRKHTDMELEVFVSGSLCISFSGNCYMSSFIGG 181 Query: 183 RCANRGRCVGSCRKEYE 199 R NRG C CRKEY+ Sbjct: 182 RSGNRGMCAQPCRKEYK 198 >FN1826 [O] COG0826 Collagenase and related proteases Length = 410 Score = 160 bits (406), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 85/204 (41%), Positives = 120/204 (58%), Gaps = 2/204 (0%) Query: 3 ELLAPAGNMEALKAAISNGCDAVYLGMQKFGARAYSSNFDVESLQEAVTYAHLRDVKIYV 62 ELLAPAGNME K A+ G DAV++G + F RA S+NF E L+EAV YAH R ++Y+ Sbjct: 8 ELLAPAGNMEKFKMALHYGADAVFMGGKMFNLRAGSNNFSDEELEEAVAYAHERGKRVYI 67 Query: 63 TMNTIVFENEVEEMKKQIRELNEIGVDGIIVQDLTAFDYIVNNFLDMEAHCSTQMGIDDL 122 T+N I +E+E + + ++ L ++GVDG+IV DL F + N ++ STQ + Sbjct: 68 TLNIIPHNDELEALPEYVKFLEKVGVDGVIVADLGVFQVVKENS-NLNISISTQASNTNW 126 Query: 123 DGTLLFKKLGAKRVVLSXX-XXXXXXXXXXXXADIPLEIFVHGALCVSYSGNCLMSGLTG 181 ++K +GAKRVVL+ DI LE+F+HGA+C++ SG CL+S Sbjct: 127 RSVKMWKDMGAKRVVLAREISLENIKEIREKVPDIELEVFIHGAMCMAISGRCLLSNYMT 186 Query: 182 FRCANRGRCVGSCRKEYELXXXIR 205 R ANRG C +CR +Y L R Sbjct: 187 GRDANRGDCAQACRWKYSLVEETR 210 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,214,388 Number of extensions: 499828 Number of successful extensions: 1174 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 1108 Number of HSP's successfully gapped: 50 Length of query: 210 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 108 Effective length of database: 26,614,363 Effective search space: 2874351204 Effective search space used: 2874351204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 113 (48.1 bits)