BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0513.0002.p (533 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2341 [O] COG0826 Collagenase and related proteases 110 4e-24 MA0538 [O] COG0826 Collagenase and related proteases 109 1e-23 MTH1763 [O] COG0826 Collagenase and related proteases 64 9e-10 MA4661 [O] COG0826 Collagenase and related proteases 51 6e-06 >CAC2341 [O] COG0826 Collagenase and related proteases Length = 787 Score = 110 bits (276), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 149/574 (25%), Positives = 235/574 (40%), Gaps = 87/574 (15%) Query: 15 SLKIEGRMKVPTYVANVVSKYRMALDHNITA---EDKENLKKTFNRTFTXXXXXXXXXFH 71 SLKIEGRMK P Y VVS YR A+D+ + E+K L K FNR Sbjct: 237 SLKIEGRMKKPEYAGGVVSSYRKAIDNKLNGDYKEEKAKLLKLFNRE----GFSRAYLLG 292 Query: 72 EDRRNITNISRPNNFGYPIGTISKIEKDRYEIKLTRPINQNDTIRISHGNEDVNLTVAKL 131 +++ P N G IG K+ D I L +N D +R DV TV Sbjct: 293 NTGKDMMAYKFPKNTGVFIG---KVISDG-SIVLKESLNLKDGVR-----NDVKGTVVSK 343 Query: 132 YNKDGGLINTADDVCYIKIKEKM-SMGDVVYKTKDFFYHKELEASLEKEFKR-FNLDIRV 189 DG + A +KI + GD +YKT D +E+E S +K+FKR LD+ + Sbjct: 344 ILLDGVEVKEAKPGEKVKIFPRFYKSGDYIYKTLDLKLTREIEESYKKDFKRKVYLDLEI 403 Query: 190 YARPDSKLVIDAE-----GLGFNYSYES------EEMLGGAINNPTTKDQVIKQFSRLND 238 + I A LG N + +M+ A +P +++V + + Sbjct: 404 EFKIGEPFKITASFSRVNDLGANEKNRAISIQVLGDMVCKAEKSPVKREKVEEHLRKTGG 463 Query: 239 TIFELHQVDYEEC--NAFIPAKLLNAARRDIVLGLYDLKLNSQQKRTKTVSAKEKISFAP 296 T FE+ +++ C +IP LN+ RR+ VL + + KR + E I + Sbjct: 464 TPFEVRNIEFT-CFDEGYIPISALNSIRRE-VLEKFQNSIQKSYKREANLKF-EGIKISK 520 Query: 297 VT----PYLTASVTNKVQYDACVSCGIKEIYFD---------------------NVVRRN 331 + P + V+NK QY A G K I D NV++ Sbjct: 521 RSEVKIPEIMVLVSNKEQYLAVKEEGFKNIIVDLFKRKCDLKLKELKDVYIKVPNVIKEE 580 Query: 332 QNDYKEKEGQ-------XXXXXXXXXXHYRDTNPFVTDYSLNVVNATSCYELYRLGAKRV 384 ND K + Y+ + DY N+ N+ + E Y+ Sbjct: 581 FNDICNKIDENIENIKGIVTVNAGIINKYKGLVNIIGDYKGNIFNSEA-LEFYKEDLDGF 639 Query: 385 TLSYELNKSQIEDLMIAYYKENDGYPALEMIVYGKTPLMFTKYCPM--------RKMNQC 436 +S EL+K++I+ L + +M++YGK M ++YCP+ K N Sbjct: 640 CISTELSKNEIKALNKCKCMDT------QMLIYGKIEAMVSEYCPIGSVFGGKSSKSNCQ 693 Query: 437 RVCKTKSYQLKDEHG-TFPIISHEDCXXXXXXXXXXXXXDELPNIK--GIEALRLNFTVE 493 C + LKD+ G +F +++ + C L IK G+ + R++F E Sbjct: 694 GTCMKSDFVLKDKMGVSFDVVTDKYCRSHIYNPLPLNLIGNLDEIKNLGVNSYRIDFIDE 753 Query: 494 SKEQVVQVI-HMASGKLNGSMNNAVFNQETDTRG 526 ++E V +V+ M +GK++G + F + + RG Sbjct: 754 NRESVKRVLSWMKNGKIDGEFSG--FTRGSYKRG 785 >MA0538 [O] COG0826 Collagenase and related proteases Length = 855 Score = 109 bits (272), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 105/379 (27%), Positives = 169/379 (44%), Gaps = 50/379 (13%) Query: 1 LNTIDYTHDLQE--IDSLKIEGRMKVPTYVANVVSKYRMALD--------HNITAEDKEN 50 LNT Y L E I+S KIEGRMK P YVA VV YR +D + ++ E++E Sbjct: 224 LNTASYLGALIEAGIESFKIEGRMKRPEYVAGVVRIYRRLIDRYFKNPARYFVSEEEQET 283 Query: 51 LKKTFNRTFTXXXXXXXXXFHEDRRNITNISRPNNFGYPIGTISKIEK--DRYEIKLTRP 108 L + FNR FT F R + + P+N G P G I ++ R +KL+ Sbjct: 284 LTQLFNRGFT-----SGYFFENPRGKLMSRENPHNRGIPAGKIVSYDRRTKRIRVKLSNT 338 Query: 109 INQNDTIRISHGN---EDVNLTVAKLYNKDGGLINTA-DDVCYIKIKEKMSMGDVVYKTK 164 + D I + + ED ++ +Y + G + + +V I + G VY+T Sbjct: 339 LRPGDGIMVENAETRPEDKGKIISSMYTEKGPVYSAGKGEVVEIPFDSRAPSGSTVYRTH 398 Query: 165 DFFYHKELEASLEKEFKRFNLDIRVYARPDSKLVIDAEG-LGFNYS------YESEEMLG 217 D K+L SL+KE + L ++ + L LG S ESE ++ Sbjct: 399 D----KKLMDSLKKESESGTLRPKIPVSLTATLTPGKPARLGIKDSDSNVVTVESEYLVE 454 Query: 218 GAINNPTTKDQVIKQFSRLNDTIFELHQVDYE-ECNAFIPAKLLNAARRDIVLGLYDLKL 276 A PT+K Q+ KQ ++L +T+FE +++ E + FIP LN R VL L +L++ Sbjct: 455 EAEKLPTSKAQIEKQLTKLGNTLFEASELNVNVEGDIFIPVGKLNELRTKAVLQLENLRV 514 Query: 277 NSQ--------------QKRTKTVSAKEKISFAPVT--PYLTASVTNKVQYDACVSCGIK 320 + +K+T+ + E + P L+ SV + + ++ G Sbjct: 515 SKWKRKPLENLQLPAPGEKKTEGIKGIEGTKAQKLAKKPLLSVSVYSPEGLEGAIAGGAD 574 Query: 321 EIYF-DNVVRRNQNDYKEK 338 +YF + + R+ + EK Sbjct: 575 CVYFGEGLFRKPKATEGEK 593 >MTH1763 [O] COG0826 Collagenase and related proteases Length = 268 Score = 63.5 bits (153), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 0/355 (0%), Positives = 0/355 (0%), Gaps = 59/355 (16%) Query: 13 IDSLKIEGRMKVPTYVANVVSKYRMALDH------NITAEDKENLKKTFNRTFTXXXXXX 66 + + V ++ ++ Sbjct: 239 QCTETVRTEEAVLATLSVFNMLTQIDEKD---- 294 Query: 67 XXXFHEDRRNITNISRPNNFGYPIGTISKIEKDRYEIKLTRPI--NQNDTIRISHGNEDV 124 Sbjct: 295 ---- 350 Query: 125 NLTVAKLYNKDGGLINTADDVCYIKIKEKMSMGDVVYKTKDFFYHKELEASLEKEFKR-- 182 Sbjct: 351 --------------- 395 Query: 183 ---FNLDIRVYARPDSKLVIDAE----GLGFNYSYESEEMLGGAINNPTTKDQVIKQFSR 235 Sbjct: 396 -- 453 Query: 236 LNDTIFELHQVDYE-ECNAFIPAKLLNAARRDIVLGLYDLKLNSQQKR------------ 282 Sbjct: 454 - 512 Query: 283 TKTVSAKEKISFAPVTPYLTASVTNKVQYDACVSCGIKEIYFDNVVRRNQNDYKE 337 Sbjct: 513 --- 564 >MA4661 [O] COG0826 Collagenase and related proteases Length = 861 Score = 50.8 bits (120), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 44/162 (27%), Positives = 79/162 (48%), Gaps = 14/162 (8%) Query: 358 PFVTDYSLNVVNATSCYELYRLGAKRVTLSYELNKSQIEDLMIAYYK-ENDGYPALEMIV 416 PFV LN+ NA + ++ GA R TLS ELN S+++++ A EN G E++V Sbjct: 684 PFVAQKDLNIFNAFTASTFFQAGAYRATLSSELNLSEMKNVCEALQACENPGQT--EILV 741 Query: 417 YGKTPLMFTKYCPMRKMNQCRVCKTKS-YQLKDEHGT-FPIISHEDCXXXXXXXXXXXXX 474 YG+ ++ T+ ++ + R+ + +S L D++G+ FP+ S Sbjct: 742 YGRELMLITENDLLKPLVDRRILRKESEVLLVDQNGSEFPVKSF----GTRTLIYNSKVL 797 Query: 475 DELPNIK-----GIEALRLNFTVESKEQVVQVIHMASGKLNG 511 D L +K G++ LRL+ ++ + +V ++ L G Sbjct: 798 DMLKYVKNMKGYGVDVLRLDLSLNTGAEVREITEAYKQALAG 839 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,066,125 Number of extensions: 1277806 Number of successful extensions: 3860 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 3860 Number of HSP's successfully gapped: 7 Length of query: 533 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 422 Effective length of database: 25,576,357 Effective search space: 10793222654 Effective search space used: 10793222654 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)