BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0509.0001.p (382 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1217 [GEPR] COG0477 Permeases of the major facilitator superf... 132 7e-31 SPy1856 [GEPR] COG0477 Permeases of the major facilitator superf... 122 8e-28 L101912 [GEPR] COG0477 Permeases of the major facilitator superf... 94 3e-19 sll1154 [GEPR] COG0477 Permeases of the major facilitator superf... 82 1e-15 BH0237 [GEPR] COG0477 Permeases of the major facilitator superfa... 60 5e-09 >lin1217 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 401 Score = 132 bits (333), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 95/331 (28%), Positives = 160/331 (48%), Gaps = 2/331 (0%) Query: 14 AEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAVSITAVIGYYWA 73 A+ L AS + + ++ + + +RL+ GK +E GR ++ G+ + + Y + Sbjct: 35 AQEELGASLGEAGFASGIYIIGTLLARLYMGKKLELFGRKQVLRFGILFFLVTTMAYLYM 94 Query: 74 GSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTSLAMSAGPLIGL 133 +I + ++R L G +G STA + + IP R GEG+ Y+GLSTSLA + GP IG+ Sbjct: 95 PTIAIMFIIRFLNGFAYGTTSTATNAIVTAYIPNSRNGEGINYYGLSTSLAAAIGPFIGM 154 Query: 134 SLLQGPGFGSLLI-STAAALVLILPLSYRLTRKLPAHHKEPAAPPVAEIRGGTFRRLFIP 192 LL F +++I ST L+ L Y + + + A ++ ++ Sbjct: 155 ILLSKTSFYTIIIFSTVIVLLTALLCFYLPVKNIVLTKEHRTALQTWTVKSFIETKVIPI 214 Query: 193 CLLNFLLSVSXXXXXXXXXXXXXEAHLEHI-AYFFLFNAVAIVVVRPLSGKIYDRFGPPA 251 + FL+ +S E +L +FF+ A+ I RP+SGK++D G Sbjct: 215 TFIAFLMGISYSSVLTFLASYAREINLVSAGTFFFVVYALVITFTRPMSGKLFDAKGEKY 274 Query: 252 LLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQTWMIQSVEPRQRGTAN 311 ++ P LF+ GL+ LS A+S+ L + G+G+G+ Q ++ EP + G Sbjct: 275 VMYPSYLFLAVGLVVLSTATSSIVLLISGGLIGLGYGTFMSNGQAVCLKVCEPHRIGIGL 334 Query: 312 GMFFNSLDLGVAIGTMILGSIALYTSFGVMY 342 +F LDLG+ IG I+G I SF +Y Sbjct: 335 STYFIGLDLGLGIGPYIMGEIHHVLSFQGIY 365 >SPy1856 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 431 Score = 122 bits (307), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 96/327 (29%), Positives = 154/327 (47%), Gaps = 12/327 (3%) Query: 14 AEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAVSITAVIGYYWA 73 A L A + Q L T ++ L + +RL GK +E GR L++ G + + Y++ Sbjct: 68 ATRELGAQTSQAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYM 127 Query: 74 GSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTSLAMSAGPLIGL 133 +I + L+R L G G+G+ STA T+ + IP R+ GEG+ ++GLSTSLA + GP +G Sbjct: 128 PTISMMYLVRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGT 187 Query: 134 SLLQGPGFG-SLLISTAAALVLILPLSYRLTRKLPAHHKEPAAPPVAEIRGGTF------ 186 +L ++I + L+ + + P + A +A+ + T Sbjct: 188 FMLDNLHIDFRMIIVLCSVLIGCVVVG---AFAFPVKNMSLNAEQLAKTKSWTVDSFIEK 244 Query: 187 RRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHI-AYFFLFNAVAIVVVRPLSGKIYD 245 + LFI + FL+ ++ E HL + AYFF+ A+ I + RP G++ D Sbjct: 245 KALFITA-IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMD 303 Query: 246 RFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQTWMIQSVEPR 305 G +L P LF+ GL L SS + + G G+G+ Q IQ V+ Sbjct: 304 AKGDKWVLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEH 363 Query: 306 QRGTANGMFFNSLDLGVAIGTMILGSI 332 + TA + LDLG+ G +LG I Sbjct: 364 RFNTAMSTYMIGLDLGLGAGPYLLGLI 390 >L101912 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 406 Score = 94.4 bits (233), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 95/336 (28%), Positives = 157/336 (46%), Gaps = 13/336 (3%) Query: 8 STLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAVSITAV 67 S++ YA L S+V ++S+F + A+ R+++G + + G L+++G + + Sbjct: 30 SSIGTYALQQLHQSTVVSLSLSSVFVIGALIGRVWTGINITRIGMKRLLYIGGLIFLVLT 89 Query: 68 IGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTSLAMSA 127 GYY +I L L+R++ G GFG+ +TA T+A ++P R GEG+GY+ LS +LA + Sbjct: 90 FGYYLTTNIPLLFLIRVIQGAGFGIGATASGTIAGHVVPASRRGEGIGYYALSVTLAAAV 149 Query: 128 GPLIGLSLLQGPGFGSLLISTAAALVLILPLSYRLTRKLPAHHKEPAAPPVAEIRGGTFR 187 GP + + + GFGSLL LV+ L + + ++ E A +A G R Sbjct: 150 GPALSIMIYSSLGFGSLLGIALGLLVITFILIFFV--RVKEFSAEERAYALAHEPKGVER 207 Query: 188 RLFIPCL----LNFLLSVSXXXXXXXXXXXXXEAHLEHI-AYFFLFNAVAIVVVRPLSGK 242 + L + FL + + + FF AV I V RP +G+ Sbjct: 208 YIEKSALPISIIAFLAGFIDSAILTGMGSFSTDLKIPLAGSLFFTMYAVLIFVSRPFTGR 267 Query: 243 IYDRFGPPALLIPGSLFIVAGLLQLSFAS------STAALFPAALCYGIGFGSMQPALQT 296 ++D G + P +F +L + A L A +G+G+G + P Q Sbjct: 268 LFDTKGDNWIFNPTFIFFAVAMLLVGLAGFFSPAIGFVVLLIAGGAFGLGYGGVAPFGQA 327 Query: 297 WMIQSVEPRQRGTANGMFFNSLDLGVAIGTMILGSI 332 I+ + G A FF LDLGV G ++LG+I Sbjct: 328 IAIRDSSKDRIGVATSTFFGFLDLGVGGGPIVLGAI 363 >sll1154 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 418 Score = 82.4 bits (202), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 90/340 (26%), Positives = 152/340 (44%), Gaps = 14/340 (4%) Query: 1 MGLQMTLSTLPAYAEGTLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGL 60 + L L TLP YA+ + A+ QV + FAL + SR++ G+ +++ GR + I +G Sbjct: 30 LSLTALLPTLPMYAQ-DVGATKQQVGWIMGSFALGLLGSRIWIGQLVDRRGRKIAIVIGA 88 Query: 61 AVSITAVIGYYWAGSILALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLS 120 V A +GY ++ AL+ +R GI +T + ++ D+ P ++ GE +GY L+ Sbjct: 89 LVVALAPLGYLLTATVGALMAIRAFHGICLAAYTTGYNSLVVDLSPPQQRGEIIGYMSLA 148 Query: 121 TSLAMSAGPLIGLSLLQGPGFGSLLISTA--AALVLILPLSYRLTRKLPAHHKE------ 172 + M GP +G + Q G+ L I+++ + L+L L R R E Sbjct: 149 VPIGMGFGPAVGAYISQSIGYEFLFIASSLLGFISLVLALQVRENRPPDQSSAEDVFGDR 208 Query: 173 PAAPPVAEIRGGTFRRLFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIA-YFFLFNAV 231 P + ++ T R + +P L+ L+ + E L A F+ A+ Sbjct: 209 PRPRSIGKLL--TSRSMAVPTLVLLLIGLLFGNLATFLPLFVREIDLATYAGVFYSVAAI 266 Query: 232 AIVVVRPLSGKIYDRFGPPALLIPGSLFI-VAGLLQLSFASSTAALFPAALCYGIGFGSM 290 A V R G + D +G L I GSL + ++ L+ A ++ L AA G G G + Sbjct: 267 ASFVPRLFLGSLADTYG-RGLFIVGSLICYLLSMVCLAQADNSWELLTAAALEGAGSGIL 325 Query: 291 QPALQTWMIQSVEPRQRGTANGMFFNSLDLGVAIGTMILG 330 P + +RG + + D G+AI I+G Sbjct: 326 LPTTIALISDRSSLEERGRVFSVCMSGFDGGIAIAAPIMG 365 >BH0237 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 387 Score = 60.5 bits (145), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 82/329 (24%), Positives = 138/329 (41%), Gaps = 23/329 (6%) Query: 17 TLQASSVQVSLVTSLFALSAIASRLFSGKAMEKGGRNLLIFLGLAVSITAVIGYYWAGSI 76 +L AS V + ++++L+ + + G +++ GR ++++G+ ++ Y A + Sbjct: 29 SLGASETLVGGIVAIYSLANMVGNIIGGHWVDRFGRKRMLYMGMLAVGGVLLLYPLAQTG 88 Query: 77 LALLLLRMLFGIGFGMASTAFPTMASDIIPIRRMGEGMGYFGLSTSLAMSAGPLIGLSLL 136 L R+L G+ G+ A + D R+ + M + G S +A GP IG Sbjct: 89 GQLFFARLLHGLAGGVLIPAAFALIGDQAKARQSRKPMAFAGASIGIAAITGPAIG---- 144 Query: 137 QGPGFGSLLISTAAALVLILP-----LSYRLTRK-LPAHHKEPAAPPVAEIRGGTFRR-- 188 G L + V IL LS LT K +P K + V+ G + Sbjct: 145 -----GILAAQSRTEYVFILVAILFFLSVGLTIKFVPESMKATSRQRVSWGHVGPLLKNV 199 Query: 189 -LFIPCLLNFLLSVSXXXXXXXXXXXXXEAHLEHIAYFFLFNAVAI----VVVRPLSGKI 243 L CL F L VS + L A L + I + + PL+ K+ Sbjct: 200 SLMQACLAAFALMVSNGTLAFALPLLVTQLGLTSAATGMLLSIYGITALFIFITPLN-KV 258 Query: 244 YDRFGPPALLIPGSLFIVAGLLQLSFASSTAALFPAALCYGIGFGSMQPALQTWMIQSVE 303 Y+R P L++ G I L+ LSF + + + YGIGF + P++ + + + Sbjct: 259 YERLYPGRLVVLGLGLIGVSLMLLSFIHTLSLAVVLMVTYGIGFAFVFPSMNQMVSTASQ 318 Query: 304 PRQRGTANGMFFNSLDLGVAIGTMILGSI 332 RG A G+F+ LGV G+ I G + Sbjct: 319 DEDRGKAYGIFYAVFSLGVVAGSFISGVV 347 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,548,395 Number of extensions: 956406 Number of successful extensions: 3822 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 3837 Number of HSP's successfully gapped: 45 Length of query: 382 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 274 Effective length of database: 25,922,359 Effective search space: 7102726366 Effective search space used: 7102726366 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 117 (49.7 bits)