BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0008.p (924 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2538 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hy... 68 7e-11 MA0813_1 [S] COG1572 Uncharacterized conserved protein 65 4e-10 lin2540 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hy... 61 7e-09 >lin2538 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hydrolases Length = 1310 Score = 68.2 bits (165), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 46/123 (37%), Positives = 65/123 (52%), Gaps = 1/123 (0%) Query: 183 EAEAASLSGGAKINTDHSGYSGSAFVDGYLTQGXXXXXXXXXXXXGIYDAALRYANASGS 242 EAE A+LSG K+ DH YSG FV G+ G G Y R AN SG Sbjct: 1019 EAEEAALSGTLKVANDHWFYSGEGFVGGFGAAGDEIKFEVDVPADGEYVLNTRTANGSGM 1078 Query: 243 TRTLSVYVNGTKVKQTSLASL-ANWDTWSTKVEALSLLSGTNTIAYKYDSTDSGNVNLDN 301 +TL +Y NGT + + S NW+ W+ +A++L +GTN I + D +GN+N+D Sbjct: 1079 PQTLDLYTNGTYNSRVTFPSEGENWNIWTDTEKAVNLKAGTNKITFLMDGETTGNINIDR 1138 Query: 302 LLL 304 +L+ Sbjct: 1139 ILI 1141 >MA0813_1 [S] COG1572 Uncharacterized conserved protein Length = 586 Score = 65.5 bits (158), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 58/149 (38%), Positives = 77/149 (51%), Gaps = 4/149 (2%) Query: 506 PDLTVT-SMSWSPSAPVETDAITLTAAVKNTGTAVSAATNVSFYLGTALAGTAPVGALAA 564 PDL + SWSP+ P + +T+TA VKN G A S AT ++ Y+ + V L++ Sbjct: 53 PDLVIENDFSWSPTTPEVGEEVTITATVKNQGDAASGATTLALYIDGSSVREWDVSELSS 112 Query: 565 GASSNVSLNIGXXXXXXXXXXXKVDEGNTVIELNEGNNSYTNPSPLTVNPVSSSDLIASP 624 G SS+VS VDE N + E NEGNN T + +TV S DLI S Sbjct: 113 GESSSVSYTWIPGSEGSVELRAVVDEDNLITESNEGNNEET--ATVTVAENSLPDLIIST 170 Query: 625 VSWTPGNPAGGNTVTFSIAVKNQGTAASA 653 S+ P P G I+VKN+GTAAS+ Sbjct: 171 FSY-PEEPESGEQQRIQISVKNEGTAASS 198 Score = 65.1 bits (157), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 48/155 (30%), Positives = 72/155 (46%), Gaps = 18/155 (11%) Query: 506 PDLTVTSMSWSPSAPVET-DAITLTAAVKNTGTAVSAATNVSFYLGTALAGTAP------ 558 PDL + + P + E + LT VKN GTA S +Y+ GTAP Sbjct: 383 PDLIIEDLV--PESEGEVGKTLNLTLKVKNQGTAPSEGVQAEYYIN----GTAPTQDDIQ 436 Query: 559 VGALAAGASSNVSLNIGXXXXXXXXXXXKVDEGNTVIELNEGNNSYTNPSPL-TVNPVSS 617 V AL+ G +V ++ +D G V EL+E NN +T + T+ P Sbjct: 437 VPALSVGEEKDVMFSLVPDREGQMEVKVHIDSGTDVYELDENNNEFTKIVDVKTIRP--- 493 Query: 618 SDLIASPVSWTPGNPAGGNTVTFSIAVKNQGTAAS 652 DLI +S P +P G+ +TF++++KN+G S Sbjct: 494 -DLIIESLSLNPASPKVGDDITFTVSIKNKGLRDS 527 Score = 57.8 bits (138), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 69/199 (34%), Positives = 95/199 (47%), Gaps = 14/199 (7%) Query: 457 YTFNPASGNSVTIPVTATVSELQLKITANSGSSAGQVAEFQIIGTPAAN--PDLTVTSMS 514 YT+ P S SV + A V E L +N G++ E T A N PDL +++ S Sbjct: 120 YTWIPGSEGSVEL--RAVVDEDNLITESNEGNN-----EETATVTVAENSLPDLIISTFS 172 Query: 515 WSPSAPVETDAITLTAAVKNTGTAVSA-ATNVSFYLGTALAGTAPVGALAAGASSNVSLN 573 + P P + + +VKN GTA S+ AT ++ Y+ + V L++G SS+VS Sbjct: 173 Y-PEEPESGEQQRIQISVKNEGTAASSRATTLALYIDGSSVREWDVPRLSSGKSSSVSYT 231 Query: 574 IGXXXXXXXXXXXKVDEGNTVIELNEGNNSYTNPSPLTVNPVSSSDLIASPVSWTPGNPA 633 VDE N + E NEGNN T + +TV S DLI S S+ P +P Sbjct: 232 WVPESEGSVELRAVVDEDNLITESNEGNNEET--ATVTVTENSLPDLIISTFSY-PEDPE 288 Query: 634 GGNTVTFSIAVKNQGTAAS 652 G + VKNQGTA S Sbjct: 289 PGEHQRIRVNVKNQGTATS 307 Score = 52.4 bits (124), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 60/199 (30%), Positives = 86/199 (43%), Gaps = 11/199 (5%) Query: 457 YTFNPASGNSVTIPVTATVSELQLKITANSGSSAGQVAEFQIIGTPAANPDLTVTSMSWS 516 YT+ P S SV + A V E L +N G++ + T + PDL +++ S+ Sbjct: 230 YTWVPESEGSVEL--RAVVDEDNLITESNEGNNEETAT---VTVTENSLPDLIISTFSY- 283 Query: 517 PSAPVETDAITLTAAVKNTGTAVSAATNVSFYLGTALAGTAPVGALAAGASSNVSLNIGX 576 P P + + VKN GTA S AT + Y+ L + L++G S S Sbjct: 284 PEDPEPGEHQRIRVNVKNQGTATSEATMLVLYIDGDLVENWAISRLSSGEISYNSYTWIP 343 Query: 577 XXXXXXXXXXKVDEGNTVIELNEGNNSYTNPSPLTVNPVSSSDLIASPVSWTPGNPAG-G 635 VD+ N V E NE NN T + + V DLI + P + G Sbjct: 344 TSEGTVELKAVVDKDNLVAESNEENNEKT--ATVAVAEDFFPDLIIEDL--VPESEGEVG 399 Query: 636 NTVTFSIAVKNQGTAASAG 654 T+ ++ VKNQGTA S G Sbjct: 400 KTLNLTLKVKNQGTAPSEG 418 >lin2540 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hydrolases Length = 1090 Score = 61.2 bits (147), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 39/125 (31%), Positives = 64/125 (51%), Gaps = 6/125 (4%) Query: 182 YEAEAASLSGGAKINTDHSGYSGSAFVDGYLTQGXXXXXXXXXXX-XGIYDAALRY-ANA 239 YEAE L+ +DH+GY+G+ FV G+ + Y +RY A Sbjct: 969 YEAEFGHLTN-VSTASDHAGYTGTGFVAGFDEEKEAVEFDIDAVDGASDYTMEVRYSAGV 1027 Query: 240 SGSTRTLSVYVNGTKVKQTSLASLANWDTWSTKVEALSLLSGTNTIAYKYDSTDSGNVNL 299 +TRT VYVNG K +Q +L A+WDTW ++L G N + + +++ D+ +N Sbjct: 1028 EDATRT--VYVNGNK-QQIALPKTADWDTWDVVEVPVTLQDGINQVVFDFEAGDTAGINF 1084 Query: 300 DNLLL 304 D++++ Sbjct: 1085 DHVVI 1089 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.309 0.125 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 59,278,798 Number of extensions: 2233761 Number of successful extensions: 5604 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 5593 Number of HSP's successfully gapped: 7 Length of query: 924 Length of database: 38,378,431 Length adjustment: 116 Effective length of query: 808 Effective length of database: 24,999,687 Effective search space: 20199747096 Effective search space used: 20199747096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 121 (51.2 bits)