BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0006.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ytkP [E] COG0031 Cysteine synthase 356 3e-98 BH3271 [E] COG0031 Cysteine synthase 352 4e-97 SA0471 [E] COG0031 Cysteine synthase 303 2e-82 BH0088 [E] COG0031 Cysteine synthase 301 8e-82 BS_cysK [E] COG0031 Cysteine synthase 278 5e-75 >BS_ytkP [E] COG0031 Cysteine synthase Length = 311 Score = 356 bits (913), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 176/276 (63%), Positives = 211/276 (76%), Gaps = 3/276 (1%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 +KLE FNPSGSVKDRAAY +I +AE +G+LKPG IIEPTSGNTGIGLAMNAAA+GYKAI Sbjct: 33 LKLEFFNPSGSVKDRAAYQMIIEAEQNGLLKPGSVIIEPTSGNTGIGLAMNAAARGYKAI 92 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 LVMPD MTKERIN+LKAYGAEVVLTP ERMPG+I KA EL +I S+IP QF+N ANP Sbjct: 93 LVMPDTMTKERINLLKAYGAEVVLTPGEERMPGSIKKAKELAEQIPNSYIPMQFDNTANP 152 Query: 121 DIHRITTAPEI---LAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSP 177 D HR TTAPEI + ++ L FVA+S EAL++ P+I + VVEP GSP Sbjct: 153 DAHRKTTAPEIARAIEEIGKPLGAFVASSGTGGTITGTGEALKELFPDITVHVVEPAGSP 212 Query: 178 VLSGGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGG 237 VLSGG+PG HKLVGTSPGF+P ILN +YDEI+++SDEDA R LA+ EGIL+GPS G Sbjct: 213 VLSGGKPGAHKLVGTSPGFIPPILNEDVYDEIIKISDEDAYTTTRRLAAEEGILVGPSSG 272 Query: 238 AAVWTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 AA + A++ A+RL K V+C+ DTGERYLS ++ Sbjct: 273 AACFAAIETAKRLSPDKVVVCMTADTGERYLSTDLW 308 >BH3271 [E] COG0031 Cysteine synthase Length = 312 Score = 352 bits (903), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 178/276 (64%), Positives = 205/276 (74%), Gaps = 3/276 (1%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 +K+EMFNPSGSVKDRAAY +I QAE DG LKPG TIIEPTSGNTGIGLAMNAAA+GY+AI Sbjct: 32 LKMEMFNPSGSVKDRAAYQMIVQAEKDGYLKPGSTIIEPTSGNTGIGLAMNAAARGYRAI 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 L MPD M++ERIN+LKAYGAEVVLT ++MPGAI KA EL EI SF+P QFEN ANP Sbjct: 92 LTMPDTMSEERINLLKAYGAEVVLTEGEKKMPGAIEKAHELVREIPNSFMPMQFENSANP 151 Query: 121 DIHRITTAPEILAQMDG---RLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSP 177 D HR TTA EI +D +L FVAAS E L++ P + I VVEP GSP Sbjct: 152 DAHRFTTAVEIKKALDSIGKKLSAFVAASGTGGTITGTGEELKKFYPNVEIHVVEPFGSP 211 Query: 178 VLSGGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGG 237 VLSGG+PGPHKLVGTSPGFVP ILN +YD I ++ DEDA E R LA EGIL+GPS G Sbjct: 212 VLSGGKPGPHKLVGTSPGFVPPILNQDVYDVIDKIKDEDAYEAARNLARLEGILVGPSSG 271 Query: 238 AAVWTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 AA + A++ A RL + V+CIA DTGERYLS +F Sbjct: 272 AACYAAMKVAERLAPDEVVVCIACDTGERYLSTDLF 307 >SA0471 [E] COG0031 Cysteine synthase Length = 310 Score = 303 bits (776), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 151/274 (55%), Positives = 194/274 (70%), Gaps = 2/274 (0%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 VKLE NP GSVKDR A +I +AE +G +KPG TI+EPTSGNTGIGLA AAKGYKA+ Sbjct: 34 VKLEYQNPGGSVKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAV 93 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 MP+ M++ER N+LKAYGAE+VLTP +E M GAI KA EL E G F PQQFEN ANP Sbjct: 94 FTMPETMSQERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEE-HGYFEPQQFENPANP 152 Query: 121 DIHRITTAPEILAQMDGR-LDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVL 179 ++H +TT PE+L Q +G+ +D F+A + L+++ P I IV +EP+ SPVL Sbjct: 153 EVHELTTGPELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVL 212 Query: 180 SGGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAA 239 SGG+PGPHKL G GF+P LNT IYD I++V ++ A+EM R +A EGIL G S GAA Sbjct: 213 SGGEPGPHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAA 272 Query: 240 VWTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 ++ A+Q+A+ LG GK V+ + P GERYLS ++ Sbjct: 273 IYAAIQKAKELGKGKTVVTVLPSNGERYLSTPLY 306 >BH0088 [E] COG0031 Cysteine synthase Length = 307 Score = 301 bits (771), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 156/273 (57%), Positives = 187/273 (68%), Gaps = 1/273 (0%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 +KLE FNP SVKDR A +I AE G LKP TI+EPTSGNTGIGLAM AAAKGYK Sbjct: 32 LKLEFFNPGSSVKDRIALAMIEAAEKSGALKPDDTIVEPTSGNTGIGLAMVAAAKGYKTK 91 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 LVMP+ M+ ER N+L+AYGAE+VLTP E M GAI KA EL E G F+PQQFEN+ANP Sbjct: 92 LVMPETMSLERRNLLRAYGAELVLTPGPEGMGGAIRKATELAQE-NGYFMPQQFENEANP 150 Query: 121 DIHRITTAPEILAQMDGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVLS 180 +IHR TT E+L Q+DG+LD FV+ L++ P + I VEP SPVLS Sbjct: 151 EIHRETTGRELLEQVDGQLDGFVSGIGTGGTITGAGGLLKENFPNLHIAAVEPADSPVLS 210 Query: 181 GGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAAV 240 GG PGPHK+ G GFVP IL+T IYDE+++VS E A E R A EGIL G S GAA+ Sbjct: 211 GGNPGPHKIQGIGAGFVPKILDTDIYDEVIKVSTEQAFEYARRAAKEEGILGGISSGAAI 270 Query: 241 WTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 + ALQ A +LG GK+V+ + P GERYLS ++ Sbjct: 271 YAALQVAEKLGPGKKVVAVIPSNGERYLSTPLY 303 >BS_cysK [E] COG0031 Cysteine synthase Length = 308 Score = 278 bits (712), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 160/274 (58%), Positives = 187/274 (68%), Gaps = 2/274 (0%) Query: 1 VKLEMFNPSGSVKDRAAYNLIRQAELDGVLKPGGTIIEPTSGNTGIGLAMNAAAKGYKAI 60 +KLE NP SVKDR +I AE +G LK G TIIEPTSGNTGIGLAM AAAKG KAI Sbjct: 33 LKLEYMNPGSSVKDRIGLAMIEAAEKEGKLKAGNTIIEPTSGNTGIGLAMVAAAKGLKAI 92 Query: 61 LVMPDNMTKERINILKAYGAEVVLTPAAERMPGAIAKALELGNEIAGSFIPQQFENQANP 120 LVMPD M+ ER N+L+AYGAE+VLTP AE M GAI KA EL E G F+PQQF N +NP Sbjct: 93 LVMPDTMSMERRNLLRAYGAELVLTPGAEGMKGAIKKAEELA-EKHGYFVPQQFNNPSNP 151 Query: 121 DIHRITTAPEILAQM-DGRLDVFVAASXXXXXXXXXXEALRQQLPEIRIVVVEPQGSPVL 179 +IHR TT EI+ Q D +LD FVA E L++ P I+I VEP SPVL Sbjct: 152 EIHRQTTGKEIVEQFGDDQLDAFVAGIGTGGTITGAGEVLKEAYPSIKIYAVEPSDSPVL 211 Query: 180 SGGQPGPHKLVGTSPGFVPAILNTGIYDEIVQVSDEDALEMVRTLASTEGILIGPSGGAA 239 SGG+PGPHK+ G GFVP ILNT +YDEI V +E+A E R A EGIL G S GAA Sbjct: 212 SGGKPGPHKIQGIGAGFVPDILNTEVYDEIFPVKNEEAFEYARRAAREEGILGGISSGAA 271 Query: 240 VWTALQEARRLGSGKRVLCIAPDTGERYLSMGIF 273 ++ ALQ A++LG GK+VL I P GERYLS ++ Sbjct: 272 IYAALQVAKKLGKGKKVLAIIPSNGERYLSTPLY 305 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,737,618 Number of extensions: 855948 Number of successful extensions: 3607 Number of sequences better than 1.0e-05: 106 Number of HSP's gapped: 3432 Number of HSP's successfully gapped: 107 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)