BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0505.0004.p (265 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value narQ [T] COG3850 Signal transduction histidine kinase, nitrate/n... 91 3e-18 ZnarQ [T] COG3850 Signal transduction histidine kinase, nitrate/... 91 3e-18 ECs3331 [T] COG3850 Signal transduction histidine kinase, nitrat... 90 3e-18 ZnarQ [T] COG3850 Signal transduction histidine kinase, nitrate/... 89 8e-18 STM2480 [T] COG3850 Signal transduction histidine kinase, nitrat... 88 2e-17 >narQ [T] COG3850 Signal transduction histidine kinase, nitrate/nitrite-specific Length = 200 Score = 90.5 bits (223), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 0/206 (0%), Positives = 0/206 (0%), Gaps = 10/206 (4%) Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETI-GQLKKNVHETNRYVRQ 120 Sbjct: 359 418 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 Sbjct: 419 --- 475 Query: 181 EAMINVRKHTRAGRVAISGREDGQG-WRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAE 239 Sbjct: 476 -- 533 Query: 240 GMGWTLAL--KSGDYGTIVEIAKKGA 263 Sbjct: 534 - 558 >ZnarQ [T] COG3850 Signal transduction histidine kinase, nitrate/nitrite-specific Length = 566 Score = 90.5 bits (223), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 105/206 (50%), Gaps = 10/206 (4%) Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETI-GQLKKNVHETNRYVRQ 120 ME R +A+ELHD +AQ L L +++ L++ A++I + +++ R +R+ Sbjct: 359 MEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQSIMADFSQALNDAYRQLRE 418 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 ++ ++ + A L +++E L + + + ++ D L L Q LL IR Sbjct: 419 LLTTFRLTLQQAD---LPSALREMLDTLQNQTSAKLTXDCRLPTLALDAQMQVHLLQIIR 475 Query: 181 EAMINVRKHTRAGRVAISGREDGQG-WRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAE 239 EA++N KH A +A+S G V I D+G GI +P G YGL IM+ERAE Sbjct: 476 EAVLNAMKHANASEIAVSCVTAPDGNHTVYIRDNGIGI--GEPKEPEGHYGLNIMRERAE 533 Query: 240 GMGWTLAL--KSGDYGTIVEIAKKGA 263 +G TL SG GT+V I+ + A Sbjct: 534 RLGGTLTFSQPSGG-GTLVSISFRSA 558 >ECs3331 [T] COG3850 Signal transduction histidine kinase, nitrate/nitrite-specific Length = 566 Score = 90.1 bits (222), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 106/206 (51%), Gaps = 10/206 (4%) Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETI-GQLKKNVHETNRYVRQ 120 ME R +A+ELHD +AQ L L +++ L++ A++I + +++ R +R+ Sbjct: 359 MEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQSIMADFSQALNDAYRQLRE 418 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 ++ ++ + A L +++E L + + + ++ +D L L Q LL IR Sbjct: 419 LLTTFRLTLQQAD---LPSALREMLDTLQNQTSAKLTLDCRLPTLALDAQMQVHLLQIIR 475 Query: 181 EAMINVRKHTRAGRVAISGREDGQG-WRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAE 239 EA++N KH A +A+S G V I D+G GI +P G YGL IM+ERAE Sbjct: 476 EAVLNAMKHANASEIAVSCVTAPDGNHTVYIRDNGIGI--GEPKEPEGHYGLNIMRERAE 533 Query: 240 GMGWTLAL--KSGDYGTIVEIAKKGA 263 +G TL SG GT+V I+ + A Sbjct: 534 RLGGTLTFSQPSGG-GTLVSISFRSA 558 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 105/206 (50%), Gaps = 10/206 (4%) Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETI-GQLKKNVHETNRYVRQ 120 ME R +A+ELHD +AQ L L +++ L++ A++I + +++ R +R+ Sbjct: 359 MEERATIARELHDSLAQVLSYLRIQLTLLKRSIPEDNATAQSIMADFSQALNDAYRQLRE 418 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 ++ ++ + A L +++E L + + + ++ D L L Q LL IR Sbjct: 419 LLTTFRLTLQQAD---LPSALREMLDTLQNQTSAKLTXDCRLPTLALDAQMQVHLLQIIR 475 Query: 181 EAMINVRKHTRAGRVAISGREDGQG-WRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAE 239 EA++N KH A +A+S G V I D+G GI +P G YGL IM+ERAE Sbjct: 476 EAVLNAMKHANASEIAVSCVTAPDGNHTVYIRDNGIGI--GEPKEPEGHYGLNIMRERAE 533 Query: 240 GMGWTLAL--KSGDYGTIVEIAKKGA 263 +G TL SG GT+V I+ + A Sbjct: 534 RLGGTLTFSQPSGG-GTLVSISFRSA 558 >STM2480 [T] COG3850 Signal transduction histidine kinase, nitrate/nitrite-specific Length = 566 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 63/207 (30%), Positives = 106/207 (51%), Gaps = 10/207 (4%) Query: 62 MEAREALAKELHDGIAQSLFLLSVRIDRLEQKHERGEMDAETI-GQLKKNVHETNRYVRQ 120 ME R +A+ELHD +AQ L L +++ L++ A++I + +++ R +R+ Sbjct: 359 MEERATIARELHDSLAQVLSYLRIQLTLLKRAIPEDNAGAQSIMADFSRALNDAYRQLRE 418 Query: 121 AISNLKVPVAGAQTSLLAQSVQEQLTQIAREVTVEVAMDWSLRDENLSPAEQAELLSCIR 180 ++ ++ + A L ++ E L + + ++ +D L L Q LL +R Sbjct: 419 LLTTFRLTLQQAD---LPSALHEMLEDLQSQTPAKLTLDCRLPTLALDAQMQVHLLQIVR 475 Query: 181 EAMINVRKHTRAGRVAISGREDGQG-WRVAISDDGAGIRHEDPFAVSGSYGLQIMKERAE 239 EA++N KH A +A+S G V I D+G GI +P +G YGL IM+ERAE Sbjct: 476 EAVLNAIKHANASEIAVSCVTAPDGDHTVYIRDNGIGI--GEPHEPAGHYGLNIMRERAE 533 Query: 240 GMGWTLAL--KSGDYGTIVEIAKKGAS 264 +G TL SG GT+V I+ + ++ Sbjct: 534 RLGGTLNFSQPSGG-GTLVSISFRSSN 559 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.130 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,020,159 Number of extensions: 607167 Number of successful extensions: 1444 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 1418 Number of HSP's successfully gapped: 41 Length of query: 265 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 161 Effective length of database: 26,383,695 Effective search space: 4247774895 Effective search space used: 4247774895 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)