BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0060.p (180 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yueJ [Q] COG1335 Amidases related to nicotinamidase 243 1e-64 SP1583 [Q] COG1335 Amidases related to nicotinamidase 197 7e-51 SA1734 [Q] COG1335 Amidases related to nicotinamidase 176 2e-44 TVN0771 [Q] COG1335 Amidases related to nicotinamidase 97 1e-20 AF2151 [Q] COG1335 Amidases related to nicotinamidase 96 2e-20 >BS_yueJ [Q] COG1335 Amidases related to nicotinamidase Length = 183 Score = 243 bits (620), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 116/181 (64%), Positives = 141/181 (77%), Gaps = 4/181 (2%) Query: 2 RALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYH 59 +ALI ID+TNDFV DG L G+P I I LT+ F+ +GDYVV+AVD H+E D YH Sbjct: 3 KALICIDYTNDFVASDGKLTCGEPGRMIEEAIVNLTKEFITNGDYVVLAVDSHDEGDQYH 62 Query: 60 PEAKLFPPHNLRGTAGRELYGSLKDVYEQNRD--SIYWMDKTRYSAFCGTDLTLKLRERG 117 PE +LFPPHN++GT G++LYG L +Y+++ ++Y+M+KTRYSAF GTDL LKLRER Sbjct: 63 PETRLFPPHNIKGTEGKDLYGKLLPLYQKHEHEPNVYYMEKTRYSAFAGTDLELKLRERQ 122 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVV 177 I ELHLAGVCTDICVLHTAVDAYNKGF IVVH+ AVASFN EGH WAL HF+ S+GA+V Sbjct: 123 IGELHLAGVCTDICVLHTAVDAYNKGFRIVVHKQAVASFNQEGHAWALSHFANSIGAQVA 182 Query: 178 R 178 Sbjct: 183 E 183 >SP1583 [Q] COG1335 Amidases related to nicotinamidase Length = 191 Score = 197 bits (501), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 101/180 (56%), Positives = 126/180 (70%), Gaps = 4/180 (2%) Query: 2 RALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYH 59 +ALI ID+T DFV G L G PA I+ IS +T+ + GDY+ +D HEEND +H Sbjct: 3 KALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFERGDYIFFTIDAHEENDCFH 62 Query: 60 PEAKLFPPHNLRGTAGRELYGSLKDVY-EQNRDS-IYWMDKTRYSAFCGTDLTLKLRERG 117 PE+KLFPPHNL GT+GR LYG L Y E DS ++WMDK YSAF GTDL ++LRER Sbjct: 63 PESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMDKRHYSAFSGTDLDIRLRERR 122 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVV 177 ++ + L GV TDICVLHTA+DAYN G+ I + + AVAS PE H +ALGHF +LGAK+V Sbjct: 123 VSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVASIWPENHQFALGHFKNTLGAKLV 182 >SA1734 [Q] COG1335 Amidases related to nicotinamidase Length = 186 Score = 176 bits (446), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 88/179 (49%), Positives = 121/179 (67%), Gaps = 4/179 (2%) Query: 2 RALIVIDFTNDFV--DGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYH 59 RAL+V+D++ DF+ DG L G+P +I I F D++ +DLH +D +H Sbjct: 4 RALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNYYQDHIFFLMDLHYLHDIHH 63 Query: 60 PEAKLFPPHNLRGTAGRELYGSLKDVYE--QNRDSIYWMDKTRYSAFCGTDLTLKLRERG 117 PE+KLFPPHN+ T+GRELYG + +YE + + +++++DKTRY +F GT L LRER Sbjct: 64 PESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFIDKTRYDSFFGTPLDSLLRERS 123 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKV 176 I ++ + GVCTDICVLHTA+ AYN G+ I V + VASFN +GH WAL HF SLGA+V Sbjct: 124 INQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVASFNQKGHEWALAHFKNSLGAEV 182 >TVN0771 [Q] COG1335 Amidases related to nicotinamidase Length = 175 Score = 97.1 bits (240), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 58/154 (37%), Positives = 87/154 (56%), Gaps = 9/154 (5%) Query: 2 RALIVIDFTNDFVDGSLPVGQPAIDIAPRISGLTQAFVDSGDYVVMAVDLHEENDPYHPE 61 + L+VID NDFV G+L + + P + +AF G + D H + DP E Sbjct: 3 KGLVVIDMLNDFVHGTLKTQESLSTVIPA-RNVLEAFRKKGLPIFFTNDAHYKEDP---E 58 Query: 62 AKLFPPHNLRGTAGRELYGSLKDVYEQNRDSIYWMDKTRYSAFCGTDLTLKLRERGITEL 121 K++ H ++G+ G ++ LK + +S + ++K YSAF GT+L LR RG+TEL Sbjct: 59 MKIWGSHAMKGSWGAQIIEELKPL-----ESEFVIEKHAYSAFYGTNLDQILRARGVTEL 113 Query: 122 HLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVAS 155 L G+ DICV HTA DA + ++I V +DAVA+ Sbjct: 114 FLIGLDADICVRHTAADALYRNYAITVVEDAVAA 147 >AF2151 [Q] COG1335 Amidases related to nicotinamidase Length = 175 Score = 96.3 bits (238), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 64/180 (35%), Positives = 91/180 (50%), Gaps = 14/180 (7%) Query: 1 MRALIVIDFTNDFVDGSLPVGQPAIDIAPRISGLTQAFVDSGDY---VVMAVDLHEENDP 57 M AL+V+D DF S G I A I T V++ V+ D H E+D Sbjct: 1 MEALVVVDMQKDFCYKS---GALYIPNAEEIFEATAKVVEAARKRMPVIFTQDWHREDDV 57 Query: 58 YHPEAKLFPPHNLRGTAGRELYGSLKDVYEQNRDSIYWMDKTRYSAFCGTDLTLKLRERG 117 E K++P H + T G E+ L Q D Y++ K RYSAF TDL L LRE G Sbjct: 58 ---EFKIWPKHCVMNTEGAEVIDELN---PQPED--YYVKKRRYSAFFATDLDLLLRELG 109 Query: 118 ITELHLAGVCTDICVLHTAVDAYNKGFSIVVHQDAVASFNPEGHLWALGHFSGSLGAKVV 177 + +L++ GV T+ICVLHTA DA +G+ + V +D + + + + L H AK++ Sbjct: 110 VKKLYICGVATNICVLHTAGDAVLRGYEVAVLKDCTKALSDYDYEYGLRHMKNVFNAKII 169 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,426,343 Number of extensions: 596989 Number of successful extensions: 982 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 963 Number of HSP's successfully gapped: 32 Length of query: 180 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 80 Effective length of database: 26,845,031 Effective search space: 2147602480 Effective search space used: 2147602480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 112 (47.8 bits)