BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0057.p (344 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ymcB [J] COG0621 2-methylthioadenine synthetase 505 e-143 BH2372 [J] COG0621 2-methylthioadenine synthetase 495 e-140 SA1134 [J] COG0621 2-methylthioadenine synthetase 455 e-128 CAC1838 [J] COG0621 2-methylthioadenine synthetase 337 2e-92 sll0996 [J] COG0621 2-methylthioadenine synthetase 289 5e-78 >BS_ymcB [J] COG0621 2-methylthioadenine synthetase Length = 211 Score = 505 bits (1301), Expect = e-143, Method: Compositional matrix adjust. Identities = 0/344 (0%), Positives = 0/344 (0%), Gaps = 1/344 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 + + + + I + K + Sbjct: 165 KVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVD 224 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 Sbjct: 225 284 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 Sbjct: 285 344 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 Sbjct: 345 404 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 Sbjct: 405 464 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEAVSI 343 Sbjct: 465 508 >BH2372 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 495 bits (1274), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/341 (0%), Positives = 0/341 (0%), Gaps = 1/341 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 I + S E+ + S+ + + Sbjct: 194 DKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAG 253 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 + + + V A + R G + V + + Sbjct: 254 ARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMD 313 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 Sbjct: 314 373 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 Sbjct: 374 433 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 Sbjct: 434 493 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAEA 340 Sbjct: 494 534 >SA1134 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 455 bits (1171), Expect = e-128, Method: Compositional matrix adjust. Identities = 0/340 (0%), Positives = 0/340 (0%), Gaps = 1/340 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKREG-MRAWVNIMYGCDKF 59 + + L + I+ V + Sbjct: 167 YQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYE 226 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 + G A+ F T L Sbjct: 227 GVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGL 286 Query: 120 MDDMRKIDIPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKYS 179 + R + + + ++ +L + V + AV + + Sbjct: 287 DPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTYPAP 346 Query: 180 RETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPRE 239 Sbjct: 347 406 Query: 240 GTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLSG 299 Sbjct: 407 466 Query: 300 RSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGDCVAE 339 Sbjct: 467 506 >CAC1838 [J] COG0621 2-methylthioadenine synthetase Length = 346 Score = 337 bits (864), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 0/337 (0%), Positives = 0/337 (0%), Gaps = 3/337 (0%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENLPKKRE-GMRAWVNIMYGCDKF 59 + + L +K + +V + A + Sbjct: 104 ALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDA 163 Query: 60 CTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDIDYTFGDL 119 ++ + + ++ Sbjct: 164 LAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNR-SFEVGLAEV 222 Query: 120 MDDMRKID-IPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVLKKMSRKY 178 + + + + + + AV + Sbjct: 223 SQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIAR 282 Query: 179 SRETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAYTFIYSPR 238 + V +T + + + E + E Sbjct: 283 RHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYT 342 Query: 239 EGTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSKNNPNVLS 298 Sbjct: 343 402 Query: 299 GRSRANKLVHFEGPKELIGTFVQVRITDTKTWYIKGD 335 Sbjct: 403 439 >sll0996 [J] COG0621 2-methylthioadenine synthetase Length = 451 Score = 289 bits (739), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 145/348 (41%), Positives = 220/348 (63%), Gaps = 13/348 (3%) Query: 1 MIFGTHNIHRLPQLVKEAYFSKELVVEVWSKEGDIIENL--PKKREGMRAWVNIMYGCDK 58 ++ G + +RL QL+++ + ++V ++ I+E++ P++ + AWVNI+YGC++ Sbjct: 103 LVMGPQHANRLDQLLEQVWAGSQVVA---TESLHIMEDITKPRRESTVSAWVNIIYGCNE 159 Query: 59 FCTYCIVPFTRGKERSRRPEDVIAEMRELARQGFKEVTLLGQNVNAYGKDFTDID----- 113 C+YC+VP RG E+SR PE + EM LA+QGFKEVTLLGQN++AYG+D Sbjct: 160 RCSYCVVPNVRGVEQSRTPEAIYGEMEVLAQQGFKEVTLLGQNIDAYGRDLPGTTPSGRH 219 Query: 114 -YTFGDLMDDMRKID-IPRIRFMTSHPRDFDDRLIEVLGKGGNLTEHIHLPVQSGSTAVL 171 +T DL+ + I+ I R+RF TSHPR F +RLI+ + + EH H+P QSG +L Sbjct: 220 LHTLTDLLYHVHDIEGIDRLRFATSHPRYFTERLIQACQELPKVCEHFHIPFQSGDNDIL 279 Query: 172 KKMSRKYSRETYLELVRKIKASVPDAVLTTDIIVGFPGETEEQFEETLSLVREVGYDMAY 231 K M R Y+RE YL+++ KI+ +PDA ++ D+IVGFPGETE QFE TL+L+ EVG+D+ Sbjct: 280 KAMKRGYTREKYLQIIEKIRRYMPDAAISADVIVGFPGETEAQFENTLNLIEEVGFDLLN 339 Query: 232 TFIYSPREGTPAASMEDNVPPEVKSERLQRLNDLIKEQSRVSNDRMXXXXXXXXXXXXSK 291 T YSPR GTPAA ++ + EVK +RLQRLN L+ Q+ + R + Sbjct: 340 TAAYSPRPGTPAAFWDNQLSEEVKGDRLQRLNHLVSTQAMERSQRYLGRVEEVLVEGENL 399 Query: 292 NNPNVLSGRSRANKLVHFEGP-KELIGTFVQVRITDTKTWYIKGDCVA 338 +P + GR+R N+L F+G EL+G V V+IT+ + + + G+ ++ Sbjct: 400 KSPGQVMGRTRGNRLTFFQGEISELLGKTVPVKITEARAFSLTGEALS 447 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,430,349 Number of extensions: 1025504 Number of successful extensions: 2252 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 2149 Number of HSP's successfully gapped: 83 Length of query: 344 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 237 Effective length of database: 26,037,693 Effective search space: 6170933241 Effective search space used: 6170933241 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)