BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0504.0056.p
         (211 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BH2372 [J] COG0621 2-methylthioadenine synthetase                     147   1e-35
SA1134 [J] COG0621 2-methylthioadenine synthetase                     107   9e-24
BS_ymcB [J] COG0621 2-methylthioadenine synthetase                    107   2e-23
CAC1838 [J] COG0621 2-methylthioadenine synthetase                     81   1e-15
alr1312 [J] COG0621 2-methylthioadenine synthetase                     78   8e-15

>BH2372 [J] COG0621 2-methylthioadenine synthetase
          Length = 346

 Score =  147 bits (371), Expect = 1e-35,   Method: Compositional matrix adjust.
 Identities = 8/136 (5%), Positives = 24/136 (17%), Gaps = 6/136 (4%)

Query: 36  FDFSDAKVISEEEGKTTYRIKGRTVQINSNPDYKEGKRRGKQDIEVIDFDNAETNKIMEE 95
                   +++                       E                     I  +
Sbjct: 32  AHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLI----IASK 87

Query: 96  FREINRHKQHYYSIATYGCQMNEHDTETMKGLLEQMGYLGTEDRNMADIILLNTCAIREN 155
               +R+      I        ++  E +   L+++     +   +        C  +  
Sbjct: 88  VSGPSRNND--KGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG 145

Query: 156 AEDKVFGELGHLKNLK 171
                      L +  
Sbjct: 146 YSWTDSAPAVSLLDTL 161


>SA1134 [J] COG0621 2-methylthioadenine synthetase
          Length = 346

 Score =  107 bits (268), Expect = 9e-24,   Method: Compositional matrix adjust.
 Identities = 10/134 (7%), Positives = 28/134 (20%), Gaps = 9/134 (6%)

Query: 39  SDAKVISEEEGKTTYRIKGRTVQINSNPDYKEGKRRGKQDIEV-IDFDNAETNKIMEEFR 97
           S  +V +   G  T+  +      ++  DY   +     D+                E +
Sbjct: 9   SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPV------PPRPETQ 62

Query: 98  EINRHKQHYYSIATYGCQMNEHDTETMKGLLEQMGYLGTEDRNMADIILLNTCAIRENAE 157
            +        +         +    +      +    G       D   +          
Sbjct: 63  GLTETYV--GNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120

Query: 158 DKVFGELGHLKNLK 171
            +      +  +  
Sbjct: 121 LQTDYLDLYQVHWP 134


>BS_ymcB [J] COG0621 2-methylthioadenine synthetase
          Length = 211

 Score =  107 bits (266), Expect = 2e-23,   Method: Compositional matrix adjust.
 Identities = 3/106 (2%), Positives = 13/106 (12%), Gaps = 7/106 (6%)

Query: 66  PDYKEGKRRGKQDIEVIDFDNAETNKIMEEFREINRHKQHYYSIATYGCQMNEHDTETMK 125
                  +        ++                   +                   +  
Sbjct: 34  IAVLLSAQATD---VSVNKAT--AKLYPVANTPAAMLELGV--EGVKTYIKTIGLYNSKA 86

Query: 126 GLLEQMGYLGTEDRNMADIILLNTCAIRENAEDKVFGELGHLKNLK 171
             + +   +  E  N                  K    + +     
Sbjct: 87  ENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGW 132


>CAC1838 [J] COG0621 2-methylthioadenine synthetase
          Length = 346

 Score = 80.9 bits (198), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 4/69 (5%), Positives = 12/69 (17%)

Query: 103 KQHYYSIATYGCQMNEHDTETMKGLLEQMGYLGTEDRNMADIILLNTCAIRENAEDKVFG 162
                  +     +        +   E   +   +      I L++   +          
Sbjct: 3   YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62

Query: 163 ELGHLKNLK 171
            L       
Sbjct: 63  GLTETYVGN 71


>alr1312 [J] COG0621 2-methylthioadenine synthetase
          Length = 346

 Score = 78.2 bits (191), Expect = 8e-15,   Method: Compositional matrix adjust.
 Identities = 3/59 (5%), Positives = 8/59 (13%)

Query: 107 YSIATYGCQMNEHDTETMKGLLEQMGYLGTEDRNMADIILLNTCAIRENAEDKVFGELG 165
           +S            T   +                   ++            +  G   
Sbjct: 8   HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.316    0.135    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 14,523,040
Number of extensions: 573344
Number of successful extensions: 866
Number of sequences better than 1.0e-05: 30
Number of HSP's gapped: 857
Number of HSP's successfully gapped: 30
Length of query: 211
Length of database: 38,378,431
Length adjustment: 102
Effective length of query: 109
Effective length of database: 26,614,363
Effective search space: 2900965567
Effective search space used: 2900965567
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 113 (48.1 bits)