BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0054.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2979 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related... 340 2e-93 YPO0328 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related... 260 1e-69 ZrhaD [G] COG0235 Ribulose-5-phosphate 4-epimerase and related e... 256 2e-68 rhaD [G] COG0235 Ribulose-5-phosphate 4-epimerase and related ep... 256 2e-68 ZrhaD [G] COG0235 Ribulose-5-phosphate 4-epimerase and related e... 256 2e-68 >lin2979 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases Length = 301 Score = 340 bits (872), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 15/266 (5%), Positives = 46/266 (17%), Gaps = 1/266 (0%) Query: 15 VEAPFIREMSEITHHMWSLGWDELNGGNVSCLLDEEEVAKYISIREPLRTIKLTFPVQEL 74 FI + +L L + I + + Sbjct: 6 SYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPG 65 Query: 75 AGKYFIVTGSGKYFRNVIKDPEANLGVLRVSGSGESVEVLWGLRDGAVPTSELASHFMSH 134 + + V+ V + + ++ Sbjct: 66 LHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNK 125 Query: 135 IERLKVDPSHRIVLHTHATNVIAMTFTHDLDELKFTKTLWEMCTECLVVFPDGVSVIPWM 194 ++ ++ L A+ + + E + ++ Sbjct: 126 VDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYI 185 Query: 195 VPGSSDIGRATADKMKDYRVVVWPQHGIFVTGATMDATFGLVETIEKAATVYNLIGGR-A 253 S + + K R + + + G + Sbjct: 186 TDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKM 245 Query: 254 IRQKITDRQLADLAAAFRVTPRAGIL 279 + + A + Sbjct: 246 VIGNKGAKIKTIGIEARKDMQEMFEA 271 >YPO0328 [G] COG0235 Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases Length = 274 Score = 260 bits (665), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 130/268 (48%), Positives = 182/268 (67%), Gaps = 9/268 (3%) Query: 19 FIREMSEITHHMWSLGWDELNGGNVSCLLDEEEVAKYIS--IREPLRTIKLTFPVQELAG 76 FI+ M + T MW GWDE NGGN+S L EEV Y ++P R ++LT P ELA Sbjct: 9 FIQGMIKATSDMWHKGWDERNGGNISLRLLAEEVEPYRRDFYQQP-RKVELTQPAPELAN 67 Query: 77 KYFIVTGSGKYFRNVIKDPEANLGVLRVSGSGESVEVLWGLRDGAVPTSELASHFMSHIE 136 +F+VTGSGK+FRNV +P NL +L+VS G + + WGL G +PTSELA+HF SHI Sbjct: 68 SWFLVTGSGKFFRNVELNPAENLVLLQVSNDGMAYHIHWGLTQGGLPTSELAAHFQSHIV 127 Query: 137 RLKVDP-SHRIVLHTHATNVIAMTFTHDLDELKFTKTLWEMCTECLVVFPDGVSVIPWMV 195 R++V ++R+++H HATN+IA+++ L+ FT+ LWE TECLVVFPDG+ ++PWMV Sbjct: 128 RMQVSGGTNRVIMHCHATNLIALSYVQKLENASFTRLLWEGSTECLVVFPDGIGIVPWMV 187 Query: 196 PGSSDIGRATADKMKDYRVVVWPQHGIFVTGATMDATFGLVETIEKAATVYNLI---GGR 252 PG+ IG TA++M+++ +V+WP HGIF +G T+D FGL++T EK+A + + GG+ Sbjct: 188 PGTDGIGTQTAEQMREHSLVLWPFHGIFGSGPTLDDAFGLIDTAEKSAEIMVKVLSMGGK 247 Query: 253 AIRQKITDRQLADLAAAFRVTPRAGILE 280 +Q I+ QL LAA F VTP A L+ Sbjct: 248 --KQTISREQLIALAARFDVTPMAAALD 273 >ZrhaD [G] COG0235 Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases Length = 274 Score = 256 bits (655), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 128/267 (47%), Positives = 180/267 (67%), Gaps = 9/267 (3%) Query: 19 FIREMSEITHHMWSLGWDELNGGNVSCLLDEEEVAKY-ISIREPLRTIKLTFPVQELAGK 77 F++ M + T W GWDE NGGN++ LD+ ++A Y + + R I L+ P+ LA Sbjct: 9 FVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAPYHDNFHQQPRYIPLSQPMPLLANT 68 Query: 78 YFIVTGSGKYFRNVIKDPEANLGVLRVSGSGESVEVLWGLRDGAVPTSELASHFMSHIER 137 FIVTGSGK+FRNV DP ANLG+++V G +LWGL + AVPTSEL +HF+SH ER Sbjct: 69 PFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGYHILWGLTNEAVPTSELPAHFLSHCER 128 Query: 138 LKV-DPSHRIVLHTHATNVIAMTFTHDLDELKFTKTLWEMCTECLVVFPDGVSVIPWMVP 196 +K + R+++H HATN+IA+T+ + D FT+ LWE TECLVVFPDGV ++PWMVP Sbjct: 129 IKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVGILPWMVP 188 Query: 197 GSSDIGRATADKMKDYRVVVWPQHGIFVTGATMDATFGLVETIEKAAT----VYNLIGGR 252 G+ +IG+ATA +M+ + +V+WP HG+F +G T+D TFGL++T EK+A VY++ G Sbjct: 189 GTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDETFGLIDTAEKSAQVLVKVYSMGG-- 246 Query: 253 AIRQKITDRQLADLAAAFRVTPRAGIL 279 ++Q I+ +L L F VTP A L Sbjct: 247 -MKQTISREELIALGKRFGVTPLASAL 272 >rhaD [G] COG0235 Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases Length = 274 Score = 256 bits (655), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 128/267 (47%), Positives = 180/267 (67%), Gaps = 9/267 (3%) Query: 19 FIREMSEITHHMWSLGWDELNGGNVSCLLDEEEVAKY-ISIREPLRTIKLTFPVQELAGK 77 F++ M + T W GWDE NGGN++ LD+ ++A Y + + R I L+ P+ LA Sbjct: 9 FVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAPYHDNFHQQPRYIPLSQPMPLLANT 68 Query: 78 YFIVTGSGKYFRNVIKDPEANLGVLRVSGSGESVEVLWGLRDGAVPTSELASHFMSHIER 137 FIVTGSGK+FRNV DP ANLG+++V G +LWGL + AVPTSEL +HF+SH ER Sbjct: 69 PFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGYHILWGLTNEAVPTSELPAHFLSHCER 128 Query: 138 LKV-DPSHRIVLHTHATNVIAMTFTHDLDELKFTKTLWEMCTECLVVFPDGVSVIPWMVP 196 +K + R+++H HATN+IA+T+ + D FT+ LWE TECLVVFPDGV ++PWMVP Sbjct: 129 IKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVGILPWMVP 188 Query: 197 GSSDIGRATADKMKDYRVVVWPQHGIFVTGATMDATFGLVETIEKAAT----VYNLIGGR 252 G+ +IG+ATA +M+ + +V+WP HG+F +G T+D TFGL++T EK+A VY++ G Sbjct: 189 GTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDETFGLIDTAEKSAQVLVKVYSMGG-- 246 Query: 253 AIRQKITDRQLADLAAAFRVTPRAGIL 279 ++Q I+ +L L F VTP A L Sbjct: 247 -MKQTISREELIALGKRFGVTPLASAL 272 Score = 256 bits (654), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 128/267 (47%), Positives = 178/267 (66%), Gaps = 9/267 (3%) Query: 19 FIREMSEITHHMWSLGWDELNGGNVSCLLDEEEVAKYISIREPL-RTIKLTFPVQELAGK 77 F++ M + T W GWDE NGGN++ LD+ ++A Y R I L+ P+ LA Sbjct: 9 FVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAPYHDNFHQQPRYIPLSQPMPLLANT 68 Query: 78 YFIVTGSGKYFRNVIKDPEANLGVLRVSGSGESVEVLWGLRDGAVPTSELASHFMSHIER 137 FIVTGSGK+FRNV DP ANLG+++V G +LWGL + AVPTSEL +HF+SH ER Sbjct: 69 PFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGYHILWGLTNEAVPTSELPAHFLSHCER 128 Query: 138 LKV-DPSHRIVLHTHATNVIAMTFTHDLDELKFTKTLWEMCTECLVVFPDGVSVIPWMVP 196 +K + R+++H HATN+IA+T+ + D FT+ LWE TECLVVFPDGV ++PWMVP Sbjct: 129 IKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVGILPWMVP 188 Query: 197 GSSDIGRATADKMKDYRVVVWPQHGIFVTGATMDATFGLVETIEKAA----TVYNLIGGR 252 G+ +IG+ATA +M+ + +V+WP HG+F +G T+D TFGL++T EK+A VY++ G Sbjct: 189 GTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDETFGLIDTAEKSAQVLVKVYSMGG-- 246 Query: 253 AIRQKITDRQLADLAAAFRVTPRAGIL 279 ++Q I+ +L L F VTP A L Sbjct: 247 -MKQTISREELIALGKRFGVTPLASAL 272 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,810,211 Number of extensions: 917901 Number of successful extensions: 1772 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1756 Number of HSP's successfully gapped: 11 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)