BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0052.p (439 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2981 [G] COG1070 Sugar (pentulose and hexulose) kinases 467 e-131 BS_yulC [G] COG1070 Sugar (pentulose and hexulose) kinases 444 e-125 BH1551 [G] COG1070 Sugar (pentulose and hexulose) kinases 369 e-102 YPO0330 [G] COG1070 Sugar (pentulose and hexulose) kinases 350 2e-96 ZrhaB [G] COG1070 Sugar (pentulose and hexulose) kinases 334 2e-91 >lin2981 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 483 Score = 467 bits (1202), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/426 (52%), Positives = 298/426 (69%), Gaps = 1/426 (0%) Query: 1 MNKHIAVDIGASSGRLVVGTLQEGKLSLEEIHRFSNGFKERGGSCFWDIDYLLDQIIVGL 60 M ++AVDIGASSGRL++G L KL LEEIHRF NGF R G W+ID L+ +I +GL Sbjct: 1 MKHYVAVDIGASSGRLILGKLVNEKLQLEEIHRFKNGFTYRDGHERWEIDQLMQEIFIGL 60 Query: 61 RQAKALGITECTLGIDTWAVDYVLLDAAGSRIQEVYAYRDRRTEGVMEEVAAILPPDTVY 120 + K LGI+EC LGIDTW VDYVL+ A+G ++ + +YRD+RT ++ + + P + +Y Sbjct: 61 EKVKQLGISECVLGIDTWGVDYVLIGASGEKLADPISYRDKRTLNAVQNLTSEYPREYIY 120 Query: 121 AKTGIQQLTFNTLYQLYAHDRKELAQADQILLVPDYLYYRLGGRKINEVTNASTTQLLNL 180 KTGIQ + NTLYQLY +R L +A++ILL+PDY+ Y L G K+ E TN+STTQ+LNL Sbjct: 121 KKTGIQFMELNTLYQLYVEERNLLERAEKILLIPDYIGYVLTGVKVAETTNSSTTQMLNL 180 Query: 181 QTGDFDSGLLSFLHLRREQFAPLTAPGESLGFVQDSLVRQYDLPRCQLICVATHDTASAV 240 + FD LLS L++ E+FAPLT G LG V+ + +YD+P C ++ VATHDTASAV Sbjct: 181 REQLFDKDLLSHLNIDVEKFAPLTDAGTYLGKVKAEWLEEYDIPNCDVVTVATHDTASAV 240 Query: 241 LGVPVQKGRSAAYISSGTWSLLGVELDQPINNPQAMAANYTNEWGAYGTYRFLKNIMGLW 300 +G P + G + A++SSGTWSL+G+EL PINN A NYTNEWGAYGTYRFLKNIMGLW Sbjct: 241 VGTPAE-GENWAFLSSGTWSLIGMELIAPINNEAAFKENYTNEWGAYGTYRFLKNIMGLW 299 Query: 301 LIQEVRRLDGERYSFXXXXXXXXXXXXFRSLIPCNATRFLNPGNMIEEIRSACAESGQPV 360 ++QE+ R+D ++SF F+ +I N RF NP NM++EI+ C E+GQ V Sbjct: 300 IVQEIARMDDYKHSFAEMAEEASNYPYFKQIINVNDARFNNPENMVDEIKLYCQETGQTV 359 Query: 361 PETPGQLARCIFDSLALSYRSYLQELEELTGTAVEVLQIVGGGANNGLLCQLTADVAGRE 420 PET G+L C++ SLAL Y L+++ E+TG +E L IVGGG+N +L QLTA +AG E Sbjct: 360 PETIGELTNCVYGSLALYYALELEKMTEITGKKIEKLYIVGGGSNVAMLNQLTAKLAGIE 419 Query: 421 VLAGPT 426 V AGP+ Sbjct: 420 VFAGPS 425 >BS_yulC [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 485 Score = 444 bits (1143), Expect = e-125, Method: Compositional matrix adjust. Identities = 217/423 (51%), Positives = 288/423 (68%), Gaps = 3/423 (0%) Query: 4 HIAVDIGASSGRLVVGTLQEGKLSLEEIHRFSNGFKERGGSCFWDIDYLLDQIIVGLRQA 63 + A+D+GASSGR++VG L EGKL ++EIHRF+NGF +R G C WDID+LL QI+ GL++ Sbjct: 3 YTAIDVGASSGRIMVGELNEGKLDIQEIHRFANGFSQRDGHCLWDIDHLLKQILQGLQKV 62 Query: 64 KALGITECTLGIDTWAVDYVLLDAAGSRIQEVYAYRDRRTEGVMEEVAAILPPDTVYAKT 123 K LG CT+GIDTWAVDYVLLD G R++E +YRDRRT+ ++++ L +Y KT Sbjct: 63 KTLGYEHCTVGIDTWAVDYVLLDEKGDRLREAISYRDRRTDHTIDKLEHTLSKAAIYQKT 122 Query: 124 GIQQLTFNTLYQLYAHDRKELAQADQILLVPDYLYYRLGGRKINEVTNASTTQLLNLQTG 183 GIQ FNT+YQL+ DR+ L + D+I+++PDYL Y L G+ + E+TN STTQLLN+ TG Sbjct: 123 GIQFQPFNTIYQLFEEDRELLKKTDKIMMIPDYLGYCLTGKAVTEITNVSTTQLLNVSTG 182 Query: 184 DFDSGLLSFLHLRREQFAPLTAPGESLGFVQDSLVRQYDLPRCQLICVATHDTASAVLGV 243 + D LL + + +QFAPLT PG LG +++ YDLP C+++ VATHDTASAV+ Sbjct: 183 NLDPELLEAVSVLEQQFAPLTEPGCELGKLRNEWFPDYDLPACKVMTVATHDTASAVIAA 242 Query: 244 P-VQKGRSAAYISSGTWSLLGVELDQPINNPQAMAANYTNEWGAYGTYRFLKNIMGLWLI 302 P V G AYISSGTWSL+GVE PI A+ NYTNE GA T RFLKNI+G+W+I Sbjct: 243 PGVNDG--WAYISSGTWSLIGVENKTPIITDLALENNYTNERGANNTIRFLKNIIGMWVI 300 Query: 303 QEVRRLDGERYSFXXXXXXXXXXXXFRSLIPCNATRFLNPGNMIEEIRSACAESGQPVPE 362 QEV++ YSF F+ I N RFLNP NMI+EI+ C ++ Q +P Sbjct: 301 QEVKQQLQADYSFQQLAEEAKKTEPFQQFINLNDKRFLNPENMIKEIQHYCRQTRQKIPR 360 Query: 363 TPGQLARCIFDSLALSYRSYLQELEELTGTAVEVLQIVGGGANNGLLCQLTADVAGREVL 422 T G+LA CI+ +LA+ Y ++ELE +T +E I+GGGA N L QLTAD++G+ V Sbjct: 361 TAGELACCIYSNLAIIYAIAIKELETITEKPIEQFHIIGGGARNDFLNQLTADMSGKAVY 420 Query: 423 AGP 425 AGP Sbjct: 421 AGP 423 >BH1551 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 467 Score = 369 bits (947), Expect = e-102, Method: Compositional matrix adjust. Identities = 196/430 (45%), Positives = 266/430 (61%), Gaps = 12/430 (2%) Query: 1 MNKHIAVDIGASSGRLVVGTLQEGKLSLEEIHRFSNGFKERGGSCFWDIDYLLDQIIVGL 60 MN +AVDIGASSGRL+VG + K+ L EIHRF N E+ G WD+D L +I GL Sbjct: 1 MNYSLAVDIGASSGRLIVGECNK-KIQLTEIHRFENQIIEKNGQFCWDVDALFSEIKTGL 59 Query: 61 RQAKALGITECTLGIDTWAVDYVLLDAAGSRIQEVYAYRDRRTEGVMEEVAAILPPDTVY 120 ++ + GI ++GIDTWAVD+VLLD + + +YRD RT+GVMEEV + +Y Sbjct: 60 KKCREAGIEPVSMGIDTWAVDFVLLDEHDKPLTDAVSYRDPRTDGVMEEVIEQFMKERLY 119 Query: 121 AKTGIQQLTFNTLYQLYAHDRKE---LAQADQILLVPDYLYYRLGGRKINEVTNASTTQL 177 +TGIQ FNT+YQLYA ++ +A L++PDY ++ L G+K NE TNA+TTQL Sbjct: 120 LETGIQFQQFNTIYQLYALKKQHPDIFKKAKSFLMIPDYFHFLLTGKKANEYTNATTTQL 179 Query: 178 LNLQTGDFDSGLLSFLHLRREQFAPLTAPGESLGFVQDSLVRQ--YDLPRCQLICVATHD 235 +N T +D ++ L + F + P ESLG ++ V + +DL ++I ATHD Sbjct: 180 VNAFTKKWDKDIIEALGFNPDMFQEIKLPTESLGKLKSEWVEEVGFDL---EVILPATHD 236 Query: 236 TASAVLGVPVQKGRSAAYISSGTWSLLGVELDQPINNPQAMAANYTNEWGAYGTYRFLKN 295 T SAV+ VP K Y+SSGTWSL+GVE PI +A+ N+TNE G +RFLKN Sbjct: 237 TGSAVVAVP--KVADTIYLSSGTWSLIGVENSFPICVTKALDYNFTNEGGMNYQFRFLKN 294 Query: 296 IMGLWLIQEVRRLDGERYSFXXXXXXXXXXXXFRSLIPCNATRFLNPGNMIEEIRSACAE 355 IMGLW+IQEVRR RYSF F+S + N RFL P NMI+EI+ C E Sbjct: 295 IMGLWMIQEVRRNYDNRYSF-AQLVELSKGISFKSTVDVNDPRFLKPTNMIKEIQRYCQE 353 Query: 356 SGQPVPETPGQLARCIFDSLALSYRSYLQELEELTGTAVEVLQIVGGGANNGLLCQLTAD 415 +GQ PE PG++A+C+F+SLA SY S + E+E++ + + ++GGG N LL QL AD Sbjct: 354 TGQQAPELPGEVAKCVFESLAESYTSAVAEIEDIFEKDFKSINVIGGGCRNELLNQLIAD 413 Query: 416 VAGREVLAGP 425 ++V AGP Sbjct: 414 RTKKDVFAGP 423 >YPO0330 [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 485 Score = 350 bits (899), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 200/426 (46%), Positives = 257/426 (60%), Gaps = 12/426 (2%) Query: 5 IAVDIGASSGRLVVGTLQEGK--LSLEEIHRFSNGFKERGGSCFWDIDYLLDQIIVGLRQ 62 +A+D+GASSGR+++ + G+ L+L E+ RF+N K GS WDID + I GL Q Sbjct: 2 VAIDLGASSGRVMLASYYPGQQQLTLREVCRFTNQIKSIDGSDVWDIDAIEQSIREGLSQ 61 Query: 63 AKALGITECTLGIDTWAVDYVLLDAAGSRIQEVYAYRDRRTEGVMEEVAAILPPDTVYAK 122 + GI ++GID+W VD+VLLD G RI + +YRD RT+GVM L + +Y + Sbjct: 62 LDSEGIALDSIGIDSWGVDFVLLDKQGKRIGQPVSYRDSRTQGVMARAQQTLGSNAIYRR 121 Query: 123 TGIQQLTFNTLYQLYAHDRKE---LAQADQILLVPDYLYYRLGGRKINEVTNASTTQLLN 179 TGIQ L FNTLYQL A ++ LA +LL+PDYL+YRL G+ E TNASTTQLLN Sbjct: 122 TGIQFLPFNTLYQLRALSEQQPHLLADVAHLLLIPDYLHYRLTGQLNWEYTNASTTQLLN 181 Query: 180 LQTGDFDSGLLSFLHLRREQFAPLTAPGESLGFVQDSLVRQYDLPRCQLICVATHDTASA 239 ++TGD+DS LL++ + FA PG ++G+ + +Q ++ VATHDTASA Sbjct: 182 IETGDWDSDLLAYAGVPAHWFAKPGKPGNTIGYWHSANGQQ-----VPVVAVATHDTASA 236 Query: 240 VLGVPVQKGRSAAYISSGTWSLLGVELDQPINNPQAMAANYTNEWGAYGTYRFLKNIMGL 299 VL P+ AAY+SSGTWSL+G E P+ + QA +N TNE GA G YR LKNIMGL Sbjct: 237 VLAAPLIDA-DAAYLSSGTWSLMGFESGTPLTHQQAQCSNITNEGGAEGRYRVLKNIMGL 295 Query: 300 WLIQEVRRLDGERYSFXXXXXXXXXXXXFRSLIPCNATRFLNPGNMIEEIRSACAESGQP 359 WL+Q + + RSLI N +RF+NP NM EI++AC E P Sbjct: 296 WLLQRATD-ELQIDDLPQLIEQAARQPACRSLINPNDSRFINPPNMCREIQNACREHQFP 354 Query: 360 VPETPGQLARCIFDSLALSYRSYLQELEELTGTAVEVLQIVGGGANNGLLCQLTADVAGR 419 VP T QLARCIFDSLA+ YR QEL L G + L IVGGG N L QL AD G Sbjct: 355 VPNTAAQLARCIFDSLAMLYRQVAQELATLRGRPISHLHIVGGGCQNQFLNQLCADACGL 414 Query: 420 EVLAGP 425 V GP Sbjct: 415 NVSMGP 420 >ZrhaB [G] COG1070 Sugar (pentulose and hexulose) kinases Length = 489 Score = 334 bits (856), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 191/426 (44%), Positives = 251/426 (58%), Gaps = 12/426 (2%) Query: 5 IAVDIGASSGRLVVGTLQEG--KLSLEEIHRFSNGFKERGGSCFWDIDYLLDQIIVGLRQ 62 +AVD+GASSGR+++ + L+L EIHRF+NG + G WD+D L I +GL + Sbjct: 7 VAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHSQNGYVTWDVDSLESAIRLGLNK 66 Query: 63 AKALGITECTLGIDTWAVDYVLLDAAGSRIQEVYAYRDRRTEGVMEEVAAILPPDTVYAK 122 GI ++GIDTW VD+VLLD G R+ AYRD RT G+M + L +Y + Sbjct: 67 VCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAYRDSRTNGLMAQAQQQLGKRDIYQR 126 Query: 123 TGIQQLTFNTLYQLYAHDRKE---LAQADQILLVPDYLYYRLGGRKINEVTNASTTQLLN 179 +GIQ L FNTLYQL A ++ + LL+PDY YRL G+ E TNA+TTQL+N Sbjct: 127 SGIQFLPFNTLYQLRALTEQQPELIPHIAHALLMPDYFSYRLTGKMNWEYTNATTTQLVN 186 Query: 180 LQTGDFDSGLLSFLHLRREQFAPLTAPGESLGFVQDSLVRQYDLPRCQLICVATHDTASA 239 + + D+D LL++ + F T PG +G + ++P ++ VA+HDTASA Sbjct: 187 INSDDWDESLLAWSGANKAWFGRPTHPGNVIGHW--ICPQGNEIP---VVAVASHDTASA 241 Query: 240 VLGVPVQKGRSAAYISSGTWSLLGVELDQPINNPQAMAANYTNEWGAYGTYRFLKNIMGL 299 V+ P+ G AAY+SSGTWSL+G E P N A+AAN TNE GA G YR LKNIMGL Sbjct: 242 VIASPL-NGSRAAYLSSGTWSLMGFESQTPFTNDTALAANITNEGGAEGRYRVLKNIMGL 300 Query: 300 WLIQEVRRLDGERYSFXXXXXXXXXXXXFRSLIPCNATRFLNPGNMIEEIRSACAESGQP 359 WL+Q V + + + R +I N RF+NP M EI++AC E+ QP Sbjct: 301 WLLQRVLQ-EQQINDLPALISATQALPACRFIINPNDDRFINPETMCSEIQAACRETAQP 359 Query: 360 VPETPGQLARCIFDSLALSYRSYLQELEELTGTAVEVLQIVGGGANNGLLCQLTADVAGR 419 +PE+ +LARCIFDSLAL Y L EL +L G L IVGGG N LL QL AD G Sbjct: 360 IPESDAELARCIFDSLALLYADVLHELAQLRGEDFSQLHIVGGGCQNTLLNQLCADACGI 419 Query: 420 EVLAGP 425 V+AGP Sbjct: 420 RVIAGP 425 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,550,674 Number of extensions: 1345310 Number of successful extensions: 2525 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 2477 Number of HSP's successfully gapped: 31 Length of query: 439 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 330 Effective length of database: 25,807,025 Effective search space: 8516318250 Effective search space used: 8516318250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)