BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0051.p (425 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP2157 [C] COG1454 Alcohol dehydrogenase, class IV 455 e-128 ZyiaY [C] COG1454 Alcohol dehydrogenase, class IV 337 2e-92 ECs4466 [C] COG1454 Alcohol dehydrogenase, class IV 337 2e-92 yiaY [C] COG1454 Alcohol dehydrogenase, class IV 334 1e-91 ZyiaY [C] COG1454 Alcohol dehydrogenase, class IV 334 1e-91 >SP2157 [C] COG1454 Alcohol dehydrogenase, class IV Length = 383 Score = 455 bits (1171), Expect = e-128, Method: Compositional matrix adjust. Identities = 222/348 (63%), Positives = 267/348 (76%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP LTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V+NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 >ZyiaY [C] COG1454 Alcohol dehydrogenase, class IV Length = 382 Score = 337 bits (865), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 0/346 (0%), Positives = 0/346 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TD L K G+AG + L+E I +YD +PNPT +NV GL L CD +IS+GG Sbjct: 38 TDNMLTKLGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I+DYEGV +S LP++A+NTTAGT++E+T +ITDE R Sbjct: 98 GSPHDCAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEAR 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK+ +SVND LMI P +LTAATGMDALTHAIEA V+ A P+TDA AL Sbjct: 158 HIKMAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACAL 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 AV +I NL V++G + +ARE M YA YVHAMAHQLGG Y+LPH Sbjct: 218 KAVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ N+K + R A A+G+ + GK+D E A+ I +I+ L+K+V IP Sbjct: 278 GVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 Sbjct: 338 383 >ECs4466 [C] COG1454 Alcohol dehydrogenase, class IV Length = 383 Score = 337 bits (865), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 171/346 (49%), Positives = 227/346 (65%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TD L K G+AG + L+E I +YD +PNPT +NV GL L CD +IS+GG Sbjct: 38 TDSMLTKLGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I+DYEGV +S LP++A+NTTAGT++E+T +ITDE R Sbjct: 98 GSPHDCAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEAR 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK+ +SVND LMI P +LTAATGMDALTHAIEA V+ A P+TDA AL Sbjct: 158 HIKMAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACAL 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 AV +I NL V++G + +ARE M YA YVHAMAHQLGG Y+LPH Sbjct: 218 KAVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ N+K + R A A+G+ + GK+D E A+ I +I+ L+K+V IP Sbjct: 278 GVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 + L +L V E D +LA N++KDAC NP T EE++ ++R + Sbjct: 338 AGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM 383 >yiaY [C] COG1454 Alcohol dehydrogenase, class IV Length = 382 Score = 334 bits (857), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 170/342 (49%), Positives = 225/342 (65%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TD L K G+AG + L+E I +YD +PNPT +NV GL L CD +IS+GG Sbjct: 38 TDNMLTKLGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I+DYEGV +S LP++A+NTTAGT++E+T +ITDE R Sbjct: 98 GSPHDCAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEAR 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK+ +SVND LMI P +LTAATGMDALTHAIEA V+ A P+TDA AL Sbjct: 158 HIKMAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACAL 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 AV +I NL V++G + +ARE M YA YVHAMAHQLGG Y+LPH Sbjct: 218 KAVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ N+K + R A A+G+ + GK+D E A+ I +I+ L+K+V IP Sbjct: 278 GVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLF 421 + L +L V E D +LA N++KDAC NP T EE++ ++ Sbjct: 338 AGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIY 379 Score = 334 bits (857), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 170/342 (49%), Positives = 225/342 (65%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TD L K G+AG + L+E I +YD +PNPT +NV GL L CD +IS+GG Sbjct: 38 TDNMLTKLGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGG 97 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I+DYEGV +S LP++A+NTTAGT++E+T +ITDE R Sbjct: 98 GSPHDCAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEAR 157 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK+ +SVND LMI P +LTAATGMDALTHAIEA V+ A P+TDA AL Sbjct: 158 HIKMAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACAL 217 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 AV +I NL V++G + +ARE M YA YVHAMAHQLGG Y+LPH Sbjct: 218 KAVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLPH 277 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ N+K + R A A+G+ + GK+D E A+ I +I+ L+K+V IP Sbjct: 278 GVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKVDIP 337 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLF 421 + L +L V E D +LA N++KDAC NP T EE++ ++ Sbjct: 338 AGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIY 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,419,978 Number of extensions: 1084487 Number of successful extensions: 2918 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 2743 Number of HSP's successfully gapped: 67 Length of query: 425 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 316 Effective length of database: 25,807,025 Effective search space: 8155019900 Effective search space used: 8155019900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)