BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0045.p (340 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1631 [H] COG0502 Biotin synthase and related enzymes 282 5e-76 STM1269 [E] COG1605 Chorismate mutase 274 2e-73 MJ0785 [H] COG0502 Biotin synthase and related enzymes 69 1e-11 CAC1356 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relat... 64 3e-10 CAC2921 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relat... 54 5e-07 >CAC1631 [H] COG0502 Biotin synthase and related enzymes Length = 211 Score = 282 bits (722), Expect = 5e-76, Method: Compositional matrix adjust. Identities = 0/317 (0%), Positives = 0/317 (0%), Gaps = 2/317 (0%) Query: 21 LYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEILDCCR 80 L+ +A + A + + G+++ +T+ Y + E I+ CR Sbjct: 35 AVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCR 94 Query: 81 EGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYTRLYAAG 140 + + T++V R A Sbjct: 95 ILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTA--FGWPTIAVDTHIFRVCNRTQFAP 152 Query: 141 ADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQSXXXXXX 200 Sbjct: 153 212 Query: 201 XXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALIPATTAMG 260 Sbjct: 213 272 Query: 261 TAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQRVKSAGF 320 Sbjct: 273 332 Query: 321 HVDMGRGDSLKHLSRSL 337 Sbjct: 333 349 >STM1269 [E] COG1605 Chorismate mutase Length = 346 Score = 274 bits (700), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 16/318 (5%), Positives = 39/318 (12%), Gaps = 2/318 (0%) Query: 2 SQQNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSG 61 A D A I A + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAK 75 Query: 62 NRTLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAAL 121 + + + + + + + + + + Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLY--QVHW 133 Query: 122 TLSVGERDDESYTRLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQ 181 + + LL A A RA + + Sbjct: 134 PQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLA 193 Query: 182 VGAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMI 241 + Q+ + + L + + Sbjct: 194 DKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAG 253 Query: 242 ALARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTG 301 A L M Sbjct: 254 ARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMD 313 Query: 302 DESAQCRFCLEQRVKSAG 319 + + Sbjct: 314 QLKTNIESLHLELSEDVL 331 >MJ0785 [H] COG0502 Biotin synthase and related enzymes Length = 375 Score = 68.6 bits (166), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 67/279 (24%), Positives = 114/279 (40%), Gaps = 15/279 (5%) Query: 20 RLYDLAHKARITAYGTAVYLRGLIEFSNICKQN--CLYCGLRSGNRTLSRY---RLQPEE 74 +L+D+A + R + + + I +NIC N CLYCG +G Y RL EE Sbjct: 64 KLFDIASRVR-DYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEE 122 Query: 75 ILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYT 134 I G + S Y ++++ + VK+ L + + +ES Sbjct: 123 IKKSAIAIEESGIKRVSCSSAHG--YQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIK 180 Query: 135 RLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQS 194 L G D ET + +L++ + P E + ++ G ++G+ G+S Sbjct: 181 ELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGI-GES 239 Query: 195 XXXXXXXXXYLQ-ELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALI 253 YL+ EL I I F P++ TP+ + + IA+ RL+ P+ I Sbjct: 240 YEDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRI 299 Query: 254 PATTAMGTAHPNGRERALQAGAN---VLMPNLSPMSVRA 289 + T T + AL GA+ ++P PM+V+ Sbjct: 300 TSPTP--TIGAELVQFALFGGASNVATVIPKNHPMNVKG 336 >CAC1356 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 472 Score = 63.9 bits (154), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 70/279 (25%), Positives = 116/279 (41%), Gaps = 36/279 (12%) Query: 20 RLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEILDCC 79 +++ LA + + YG + + + SN C C+YC N+ ++R +L E D Sbjct: 67 KMFKLAREIKQKFYGNRIVMFAPLYLSNYCVNGCVYCPYHHKNKHIARKKLSQE---DVK 123 Query: 80 REGYTL---GYRTFVLQSGEDDW-----YTTDKLVQLITSVKQQFPDAAL---TLSVGER 128 RE L G++ L++GED Y D ++ I S+K + + A+ +++ Sbjct: 124 RETIALQDMGHKRLALEAGEDPVNNPIEYILD-CIKTIYSIKHK--NGAIRRVNVNIAAT 180 Query: 129 DDESYTRLYAAGADRFLLRHETASASLYRALHPT---MEYXXXXXXXXXXQRIGY-QVGA 184 E+Y +L AG ++L ET + Y LHPT +Y G VG Sbjct: 181 TVENYKKLKDAGIGTYILFQETYNKKSYEELHPTGPKHDYAYHTEAMDRAMEGGIDDVGI 240 Query: 185 GFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPF---LPHRDTPLRDEQPGTVENTLD-- 239 G + GL + + L+ M G+GP +P R P D P N + Sbjct: 241 GVLFGL-NMYKYDFVGLLMHAEHLEAAM-GVGPHTISVP-RIRPADDIDPENFSNAISDE 297 Query: 240 ----MIALARLMVPDALIPATTAMGTAHPNGRERALQAG 274 ++A+ R+ VP + +T RER L+ G Sbjct: 298 IFEKIVAIIRIAVPYTGMIVST---RESKKTRERVLELG 333 >CAC2921 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 368 Score = 53.5 bits (127), Expect = 5e-07, Method: Compositional matrix adjust. Identities = 45/157 (28%), Positives = 71/157 (45%), Gaps = 7/157 (4%) Query: 8 DLISHLEGALRRRLYDLAHKAR---ITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRT 64 D + L A + L +A KAR + +G V L I +N C C YCG N+ Sbjct: 40 DFLKLLSPAAEKYLEHMAQKARELSLKNFGKTVVLYTPIYIANYCVNGCAYCGYNVKNK- 98 Query: 65 LSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQ-LITSVKQQFPDAALTL 123 + R +L EEI + R Y+ G R +L +GE T ++ I +K+ F +++ + Sbjct: 99 IKRKQLTMEEIEEEARAIYSSGMRNIILLTGESKVQTPVSYIKDAIKLLKKYF--SSICI 156 Query: 124 SVGERDDESYTRLYAAGADRFLLRHETASASLYRALH 160 + + Y L AGAD + ET + Y +H Sbjct: 157 EIYPLEVNEYRELVEAGADSLTIYQETYNEEKYSKVH 193 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,412,396 Number of extensions: 930986 Number of successful extensions: 1448 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1448 Number of HSP's successfully gapped: 6 Length of query: 340 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 233 Effective length of database: 26,037,693 Effective search space: 6066782469 Effective search space used: 6066782469 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)