BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0041.p (357 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3870 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 202 7e-52 PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 200 3e-51 MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 182 8e-46 MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 175 8e-44 Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 175 9e-44 >PA3870 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 329 Score = 202 bits (514), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 126/329 (38%), Positives = 176/329 (53%), Gaps = 31/329 (9%) Query: 12 DSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSP--EPLTFDEIATVVSVLADMGVTK 69 D GR +Y+R+SVTDRC+ C YC + D F P + LT +EI V + GV K Sbjct: 6 DGQGRQIDYLRLSVTDRCDFRCVYCMAE--DMRFLPRQQVLTLEEIERVARLFVAGGVRK 63 Query: 70 VRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLTDVNISLDS 129 +RLTGGEPL+RP + L RL A+ G+ + +T+NG L AR L +AGL+ +NISLD+ Sbjct: 64 LRLTGGEPLVRPGIVGLCERLAALPGLRELCMTSNGSQLVRYARPLYDAGLSRLNISLDT 123 Query: 130 LQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLTLKYPL 189 L P RF IT G++ +VL I ++ AGF R+K+N VVMKG N DE+ + + + L Sbjct: 124 LDPLRFRAITRNGELDKVLAGIDAAQAAGFRRIKLNAVVMKGRNADEVPALVDFAIARGL 183 Query: 190 DIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGRDVTMDT 249 DI FIE MP+G+ G + G E CS D R +D+ Sbjct: 184 DISFIEEMPLGQ--VGRERG------------------ESFCSSDEVRALIAQRHALLDS 223 Query: 250 TDS--GPARYYR-IKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWNVRP 306 + GPARY R ++ + G I P S +FC TCNRLR+T G + CL ++R Sbjct: 224 AEQSGGPARYVRLVEHPQTRIGFISPHSHNFCATCNRLRLTVEGRLLLCLGHEHSLDLRA 283 Query: 307 YIGEAEL----LKQLFLRSLEHKPGRHDM 331 + L L + +L+ KP RH+ Sbjct: 284 LLRRHPLHDGPLLEAIGEALQRKPARHEF 312 >PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 331 Score = 200 bits (509), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 131/364 (35%), Positives = 187/364 (51%), Gaps = 43/364 (11%) Query: 4 LSDAQALADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSP--EPLTFDEIATVVSV 61 +SD++ L D FGR Y+R+SVTDRC+ C YC + D F P + L+ +E+ V Sbjct: 1 MSDSR-LVDPFGRRITYLRLSVTDRCDFRCTYCMSE--DMQFLPRDQVLSLEELYAVADA 57 Query: 62 LADMGVTKVRLTGGEPLLRPHLEELVARLHAVSGIERIGLTTNGILLASKARVLREAGLT 121 +GV ++R+TGGEPL+R + L+ARL + +E + +TTNG L +A L+ AG+ Sbjct: 58 FIGLGVRRIRITGGEPLVRKGITGLLARLGQRAELEDLAITTNGSQLRERAAELKAAGVR 117 Query: 122 DVNISLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFL 181 +N+SLDSLQ ERFA T + +VL I ++ AGFER+K+N VV KG NDDEI + Sbjct: 118 RLNVSLDSLQRERFAAFTRSDRLEQVLDGIQAAREAGFERIKLNCVVQKGRNDDEILDLV 177 Query: 182 QLTLKYPLDIRFIEYMPIGRGTEGWQAGYM----PLGGILERCSRAGWAYEPVCSDDPGH 237 + L LDI FIE MP+G T + + + I+ER W P + Sbjct: 178 EYALANQLDISFIEEMPLGSITSHRRELTLCTSDEVRAIIER----RWPLTPSLA----- 228 Query: 238 GAAPGRDVTMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLY 297 GP+RYY+I + G I P S +FC CNR+R+TA G + CL Sbjct: 229 ------------RSGGPSRYYQIGDEPSRIGFISPHSNNFCGDCNRVRVTAEGKLVLCLG 276 Query: 298 WNDEWNVRPYI----GEAELLKQLFLRSLEHKPGRHDMPDRLKESGVVRPVQENTVRQMS 353 ++R + G+ E L + +L KP RH + Q +R MS Sbjct: 277 HEGALDLRELLRSHPGDRERLSAALVAALNLKPERHHFDAQ---------EQVQVLRFMS 327 Query: 354 QIGG 357 GG Sbjct: 328 MTGG 331 >MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 395 Score = 182 bits (462), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 113/328 (34%), Positives = 171/328 (52%), Gaps = 28/328 (8%) Query: 10 LADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEP--LTFDEIATVVSVLADMGV 67 + D GR +R+SV D+CNL C YC P +A+ A+ P L+ DEI+ +V +GV Sbjct: 54 IRDRCGRTMGDLRLSVIDQCNLRCRYCMP-EAEYAWLPRADLLSVDEISLIVDAFIAVGV 112 Query: 68 TKVRLTGGEPLLRPHLEELV----ARLHAVSGIERIGLTTNGILLASKARVLREAGLTDV 123 K+RLTGGEPL+R L ++ A++ SG++ + +TTNG+LLA +AR L+ AG+ + Sbjct: 113 DKIRLTGGEPLIRSDLAAIIEVISAKVGDGSGLQDLAITTNGVLLADQARKLKSAGMRRI 172 Query: 124 NISLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQL 183 ISLD+L+P+RF I+ G +V+ I + AGF K++ VV++G NDDE+ ++ Sbjct: 173 TISLDTLRPDRFKAISQRGTHYKVIEGIEAVAAAGFTDTKLDSVVIRGFNDDELSDLIEF 232 Query: 184 TLKYPLDIRFIEYMPIGRGTEGWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGR 243 ++RFIEYM +G T+ +L S G Y P+ + Sbjct: 233 ARNVNAEVRFIEYMDVGGATQWSMDKVFTKAQML---STLGKKYGPIAA----------- 278 Query: 244 DVTMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWN 303 + DS PA YR+ FG+I + FC TC+R R+TA G CLY N Sbjct: 279 ---LPKYDSAPANRYRLPDGT-TFGIIASTTEPFCATCDRSRLTADGIWLHCLYALSGIN 334 Query: 304 VRPYI---GEAELLKQLFLRSLEHKPGR 328 +R + A + Q+ R + R Sbjct: 335 LRESVRAGASANDVVQILQRGWRDRANR 362 >MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 368 Score = 175 bits (444), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 106/302 (35%), Positives = 157/302 (51%), Gaps = 25/302 (8%) Query: 10 LADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEPL-TFDEIATVVSVLADMGVT 68 + D R+ +R+SV D+CNL C YC P++ + L + EI+ +V V +GV+ Sbjct: 27 VKDRCRRMMGDLRLSVIDQCNLRCRYCMPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVS 86 Query: 69 KVRLTGGEPLLRPHLEELVARLHAV----SGIERIGLTTNGILLASKARVLREAGLTDVN 124 KVR+TGGEPL+RP L E+V L A SG+ + +TTNG+LLA + L+ AG+ + Sbjct: 87 KVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGMKRIT 146 Query: 125 ISLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLT 184 +SLD+LQPERF I+ +V+ I + AGF K++ VM+G N DE+ ++ Sbjct: 147 VSLDTLQPERFKAISQRNSHDKVIAGIKAVAAAGFTDTKIDTTVMRGANHDELADLIEFA 206 Query: 185 LKYPLDIRFIEYMPIGRGTE-GWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGR 243 ++RFIEYM +G T W+ + +LE + EP+ P H Sbjct: 207 RTVNAEVRFIEYMDVGGATHWAWEKVFTK-ANMLESLEKRYGRIEPL----PKH------ 255 Query: 244 DVTMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWN 303 D+ PA Y + FG+I + FC TC+R R+TA G CLY N Sbjct: 256 -------DTAPANRYALPDGT-TFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGIN 307 Query: 304 VR 305 +R Sbjct: 308 LR 309 >Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 359 Score = 175 bits (444), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 106/302 (35%), Positives = 157/302 (51%), Gaps = 25/302 (8%) Query: 10 LADSFGRIHNYVRISVTDRCNLSCEYCRPDQADAAFSPEPL-TFDEIATVVSVLADMGVT 68 + D R+ +R+SV D+CNL C YC P++ + L + EI+ +V V +GV+ Sbjct: 18 VKDRCRRMMGDLRLSVIDQCNLRCRYCMPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVS 77 Query: 69 KVRLTGGEPLLRPHLEELVARLHAV----SGIERIGLTTNGILLASKARVLREAGLTDVN 124 KVR+TGGEPL+RP L E+V L A SG+ + +TTNG+LLA + L+ AG+ + Sbjct: 78 KVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGMKRIT 137 Query: 125 ISLDSLQPERFARITGGGDVGRVLHAIGSSLNAGFERVKVNMVVMKGINDDEIESFLQLT 184 +SLD+LQPERF I+ +V+ I + AGF K++ VM+G N DE+ ++ Sbjct: 138 VSLDTLQPERFKAISQRNSHDKVIAGIKAVAAAGFTDTKIDTTVMRGANHDELADLIEFA 197 Query: 185 LKYPLDIRFIEYMPIGRGTE-GWQAGYMPLGGILERCSRAGWAYEPVCSDDPGHGAAPGR 243 ++RFIEYM +G T W+ + +LE + EP+ P H Sbjct: 198 RTVNAEVRFIEYMDVGGATHWAWEKVFTK-ANMLESLEKRYGRIEPL----PKH------ 246 Query: 244 DVTMDTTDSGPARYYRIKGAAGQFGLIDPVSRHFCMTCNRLRITASGHIKPCLYWNDEWN 303 D+ PA Y + FG+I + FC TC+R R+TA G CLY N Sbjct: 247 -------DTAPANRYALPDGT-TFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGIN 298 Query: 304 VR 305 +R Sbjct: 299 LR 300 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,393,572 Number of extensions: 1168486 Number of successful extensions: 3708 Number of sequences better than 1.0e-05: 51 Number of HSP's gapped: 3667 Number of HSP's successfully gapped: 51 Length of query: 357 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 249 Effective length of database: 25,922,359 Effective search space: 6454667391 Effective search space used: 6454667391 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)