BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0037.p (651 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO0344 [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 876 0.0 fdhF [C] COG0243 Anaerobic dehydrogenases, typically selenocyste... 807 0.0 MJ1353m [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 543 e-154 PAB2442 [C] COG0243 Anaerobic dehydrogenases, typically selenocy... 466 e-131 PH1353 [C] COG0243 Anaerobic dehydrogenases, typically selenocys... 452 e-127 >YPO0344 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 876 bits (2264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/649 (0%), Positives = 0/649 (0%), Gaps = 6/649 (0%) Query: 3 RKEG-VLQEVSWEEAIGFTAEKLNAIKEKYGPDAIMGTGSARGPGNEANYVMQKFMRAVI 61 + + + T + + P + G + +F Sbjct: 69 TARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTMPEH 128 Query: 62 GTNNIDHCARVCHGPSVAGLTYSLGDGAMSNSIPEIENTDLLLIFGYNAAVTHPIVARRI 121 ++ + S + + D + Sbjct: 129 KFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNETRPSQ 188 Query: 122 VKAKQKGAVLIVADPRKTESARIADTWLPLKGGTNMALVNAFGHVLIEEELYDKDYVSNF 181 K ++ A + + + G + G +E Y + Sbjct: 189 DKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTVYGTCM 248 Query: 182 VQGFDEYKANVEKYTPEYAERITGVRAADIRKVMRQYAKSGKAMILYGMGXXXXCQFAQA 241 Sbjct: 249 --- 305 Query: 242 VDVVKGLASLALLTGHFGRESVGIGPVRGQNNVQGSCDMGALPNVYPGYQSVEDAKIRKK 301 Sbjct: 306 365 Query: 302 FEKAWGTS-LPDKTGYRLTEVPHLILKENKIKAYYIFGEDPVQSDPNAAELREALDQLEL 360 Sbjct: 366 - 424 Query: 361 VIVQDIFMNKTALHADVILPATSWGEHDGVYSSADRGFQRIRKAIDPPGDVKPDWQIISE 420 Sbjct: 425 484 Query: 421 VSTAMGYPMAYKNSEEIWDEMRKLCPSFAGASYSKMEALGGVQWPCPSEDHPGTPYLYQG 480 Sbjct: 485 544 Query: 481 NRFTTPSGKGQLFACEWRAPLEQPDAEYPLTLSTVREVGHYSVRTMTGNCRALSQLSDEP 540 Sbjct: 545 604 Query: 541 GNIQISLEDAAEHGIADGQLVQVYSRRGHIMARAQVSGRIKKGATYMTYHFWIGACNELT 600 Sbjct: 605 664 Query: 601 VDYLDPVSKTPEFKYCAVRIEAISDQAQAEALVRKQYAELRKQMLVGAV 649 Sbjct: 665 713 >fdhF [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 807 bits (2084), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/654 (0%), Positives = 0/654 (0%), Gaps = 10/654 (1%) Query: 1 MIRKE--GVLQEVSWEEAIGFTAEKLNAIKEKYGPDAIMGTGSARGPGNEANYVMQKFMR 58 + + + + T + + P + G + +F Sbjct: 66 LRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRSPEWSAFGIRPPLRLGEFTM 125 Query: 59 AVIGTNNIDHCARVCHGPSVAGLTYSLGDGAMSNSIPEIENTDLLLIFGYNAAVTHPIVA 118 ++ + S + + D + Sbjct: 126 PEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNETR 185 Query: 119 RRIVKAKQKGAVLIVADPRKTESARIADTWLPLKGGTNMALVNAFGHVLIEEELYDKDYV 178 K ++ A + + + G + G +E Y Sbjct: 186 PSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTVYG 245 Query: 179 SNFVQGFDEYKANVEKYTPEYAERITGVRAADIRKVMRQYAKSGKAMILYGMGXXXXCQF 238 Sbjct: 246 --- 302 Query: 239 AQAVDVVKGLASLALLTGHFGRESVGIGPVRGQNNVQGSCDMGALPNVYPGYQSVEDAKI 298 Sbjct: 303 362 Query: 299 RKKFEKAWGT-SLPDKTGYRLTEVPHLILKENKIKAYYIFGEDPVQSDPNAAELREALDQ 357 Sbjct: 363 - 421 Query: 358 LELVIVQDIFMNKTALHADVILPATSWGEHDGVYSSADRGFQRIRKAIDPPGDVKPDWQI 417 Sbjct: 422 481 Query: 418 ISEVSTAMGYPMAYKNSEEIWDEMRKLCPSFAGASYSKMEALGGVQWPC--PSEDHPGTP 475 Sbjct: 482 541 Query: 476 YLYQGNRFTTPSGKGQLFACEWRAPLEQPDAEYPLTLSTVREVGHYSVRTMTGNCRALSQ 535 Sbjct: 542 - 600 Query: 536 LSDEPGNIQISLEDAAEHGIADGQLVQVYSRRGHIMARAQVSGRIKKGATYMTYHFWIGA 595 Sbjct: 601 660 Query: 596 CNELTVDYLDPVSKTPEFKYCAVRIEAISDQAQAEALVRKQYAELRKQMLVGAV 649 Sbjct: 661 714 >MJ1353m [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 543 bits (1400), Expect = e-154, Method: Compositional matrix adjust. Identities = 0/626 (0%), Positives = 0/626 (0%), Gaps = 20/626 (3%) Query: 1 MIRKEGVLQEVSWEEAIGFTAEKLNAIKEKYGPDAIMGTGSARGPGNEANYVMQKFMRAV 60 +R + + + +K + + + Sbjct: 65 ALRLTARPILDIALQYRFDSQQTFTRAFKK------QFAQTPALYRRSPEWSAFGIRPPL 118 Query: 61 IGTNNIDHCARVCHGPSVAGLTYSLGDGAMSNSIPEIENTDLLLIFGYNAAVTHPIVARR 120 + + + I + + + I Sbjct: 119 RLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVL 178 Query: 121 IVKAKQKGAVLIVADPRKTESARIADTWLPLKGGTNMALVNAFGHVLIEEELYDKDYVSN 180 + + + + + +A T ++ G ++ V Sbjct: 179 YGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQE 238 Query: 181 FVQGFDEYKANVEKYTPEYAERITGVRAADIRKVMRQYAKSGKAMILYGMGXXXXCQFAQ 240 Sbjct: 239 --- 295 Query: 241 AVDVVKGLASLALLTGHFGRESVGIGPVRGQNNVQGSCDMGALPNVYPGYQSVEDAKIRK 300 Sbjct: 296 -- 353 Query: 301 KFEKAWGTSLPDKTGYRLTEVPHLILKENK-IKAYYIFGEDPVQSDPNAAELREALDQLE 359 Sbjct: 354 --- 410 Query: 360 LVIVQDIFMNKTALHADVILPATSWGEHDGVYSSADRGFQRIRKAIDPPGDVKPDWQIIS 419 Sbjct: 411 470 Query: 420 EVSTAMGY--PMAYKNSEEIWDEMRKLCPSFAGASYSKMEALGGVQWPCPSEDHPGTPYL 477 Sbjct: 471 - 529 Query: 478 YQGNRFTTPSGKGQLFACEWRAPLEQPDAEYPLTLSTVREVGHYSVRTMTGNCRALSQLS 537 Sbjct: 530 - 588 Query: 538 DEPGNIQISLEDAAEHGIADGQLVQVYSRRGHIMARAQVSGRIKKGATYMTYHFWIGACN 597 Sbjct: 589 - 647 Query: 598 ELTVDYLDPVSKTPEFKYCAVRIEAI 623 Sbjct: 648 673 >PAB2442 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 466 bits (1198), Expect = e-131, Method: Compositional matrix adjust. Identities = 0/615 (0%), Positives = 0/615 (0%), Gaps = 24/615 (3%) Query: 11 VSWEEAIGFTAEKLNAIKEKYGPDAIMGTGSARGPGNEANYVMQKFMRAVIGTNNIDHCA 70 + + + A R + + ++ +R + +H Sbjct: 73 ILDIALQYRFDSQQTFTRAFKKQFAQTPALYRR-SPEWSAFGIRPPLR-LGEFTMPEHKF 130 Query: 71 RVCHGPSVAGLTYSLGDGAMSNSIPEIENTDLLLIFGYNAAVTHPIVARRIVKAKQKGAV 130 + G+T S S E A T P V + + + Sbjct: 131 VTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGLNETRPSQDK 190 Query: 131 LIVADPRKTESARIADTWLPLKGGTNMALVNAFGHVLIEEELYDKDYVSNFVQGFDEYKA 190 + T + + G + L + E L Sbjct: 191 DDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFILTVYGTCMPM 250 Query: 191 NVEKYTPEYAERITGVRAADIRKVMRQYAKSGKAMILYGMGXXXXCQFAQAVDVVKGLAS 250 Sbjct: 251 --- 307 Query: 251 LALLTGHFGRESVGIGPVRGQNNVQGSCDMGALPNVYPGYQSVEDAKIRKKFEKAWGT-S 309 Sbjct: 308 - 366 Query: 310 LPDKTGYRLTEVPHLILKENKIKAYYIFGEDPVQSDPNAAELREALDQLELVIVQDIFMN 369 Sbjct: 367 - 425 Query: 370 KTALHADVILPATSWGEHDGVYSSADRGFQRIRKAIDPPGDVKPDWQIISEVSTAMGYP- 428 Sbjct: 426 485 Query: 429 MAYKNSEEIWDEMRKLCPSFAGASYSKMEALGGVQWPCPSEDHPGTPYLYQGNRFTTPSG 488 Sbjct: 486 --------- 536 Query: 489 KGQLFACEWRAPLEQPDAEYPLTLSTVREVGHYSVRTMTGNCRALSQLSDEPGNIQISLE 548 Sbjct: 537 - 595 Query: 549 DAAEHGIADGQLVQVYSRRGHIMARAQVSGRIKKGATYMTYHFWIGACNELTVDYLDPVS 608 Sbjct: 596 ----- 650 Query: 609 KTPEFKYCAVRIEAI 623 Sbjct: 651 665 >PH1353 [C] COG0243 Anaerobic dehydrogenases, typically selenocysteine-containing Length = 289 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 0/628 (0%), Positives = 0/628 (0%), Gaps = 26/628 (4%) Query: 2 IRKEG-VLQEVSWEEAIGFTAEKLNAIKEKYGPDAIMGTGSARGPGNEANYVMQKFMRAV 60 + + F +++ K P A + Sbjct: 64 VALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPALYRRS-PEWSAFGIRPPLRL-G 121 Query: 61 IGTNNIDHCARVCHGPSVAGLTYSLGDGAMSNSIPEIENTDLLLIFGYNAAVTHPIVARR 120 T + P + + F NA P++ Sbjct: 122 EFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMRYQFWHDFLGNAPTIPPVLYGL 181 Query: 121 IVKAKQKGAVLIVADPRKTESARIADTWLPLKGGTNMALVNAFGHVLIEEELYDKDYVSN 180 + T A+ L G M + E Sbjct: 182 NETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVMLQGGEYVMFTYEGLGTGVQEFIL 241 Query: 181 FVQGFDEYKANVEKYTPEYAERITGVRAADIRKVMRQYAKSGKAMILYGMGXXXXCQFAQ 240 Sbjct: 242 --- 298 Query: 241 AVDVVKGLASLALLTGHFGRESVGIGPVRGQNNVQGSCDMGALPNVYPGYQSVEDAKIRK 300 Sbjct: 299 - 357 Query: 301 KFEKAWGT-SLPDKTGYRLTEVPHLILKENKIKAYYIFGEDPVQSDPNAAELREALDQLE 359 Sbjct: 358 - 416 Query: 360 LVIVQDIFMNKTALHADVILPATSWGEHDGVYSSADRGFQRIRKAIDPPGDVKPDWQIIS 419 Sbjct: 417 476 Query: 420 EVSTAMGYP-MAYKNSEEIWDEMRKLCPSFAGASYSKMEALGGVQWPCPSEDHPGTPYLY 478 Sbjct: 477 --------- 527 Query: 479 QGNRFTTPSGKGQLFACEWRAPLEQPDAEYPLTLSTVREVGHYSVRTMTGNCRALSQLSD 538 Sbjct: 528 - 586 Query: 539 EPGNIQISLEDAAEHGIADGQLVQVYSRRGHIMARAQVSGRIKKGATYMTYHFWIGACNE 598 Sbjct: 587 ----- 641 Query: 599 LTVDYLDPVSKTPEFKYCAVRIEAISDQ 626 Sbjct: 642 - 668 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 50,098,225 Number of extensions: 2174593 Number of successful extensions: 4123 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 3702 Number of HSP's successfully gapped: 191 Length of query: 651 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 538 Effective length of database: 25,345,689 Effective search space: 13635980682 Effective search space used: 13635980682 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)