BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0034.p (298 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl997 [S] COG1917 Uncharacterized conserved protein, contains d... 253 3e-67 YGR256w [G] COG0362 6-phosphogluconate dehydrogenase 176 4e-44 YHR183w [G] COG0362 6-phosphogluconate dehydrogenase 162 8e-40 BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase 159 7e-39 STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily 144 2e-34 >AGl997 [S] COG1917 Uncharacterized conserved protein, contains double-stranded beta-helix domain Length = 82 Score = 253 bits (645), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 0/334 (0%), Positives = 0/334 (0%), Gaps = 37/334 (11%) Query: 1 MKVGLIGLGKMGANLGRNLLDHGHEVVAFDVNREAVSELESKGAAGADSLAGLVSKLGVP 60 M L+ + + G E V + + + + + V L Sbjct: 1 MAPNLLYRWRRLLSEGGAAAVDSDEPVVGNSEVKKLEDRVRELERMLGRKTMEVEILREA 60 Query: 61 RIAWIMVPHAFVDSVIEELAPLLSKGDIIIEAGNSQYKESIRRHGELAQQGIYFLDAGTS 120 Sbjct: 61 120 Query: 121 GGMEGARNGACYMVGGDEEAWAVAEPLFRDTA---------------VQDGYLFAGKPGS 165 Sbjct: 121 180 Query: 166 GHFLKMVHNGIEYGMMAAIGEGFEVLEKSG----------------------FDYNFEQV 203 Sbjct: 181 240 Query: 204 ARVWNNGSVIRSWLMELVERAFSKDAKLDEIKGVMHSSGEGRWTLETAFDLQAATPVIAM 263 Sbjct: 241 300 Query: 264 ALLMRYRSLETDTFTGKVVAALRNEFGGHAVETK 297 Sbjct: 301 334 >YGR256w [G] COG0362 6-phosphogluconate dehydrogenase Length = 492 Score = 176 bits (446), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 114/294 (38%), Positives = 164/294 (55%), Gaps = 26/294 (8%) Query: 3 VGLIGLGKMGANLGRNLLDHGHEVVAFD-----VNREAVSELESKGAAGADSLAGLVSKL 57 +GL+GL MG NL N DHG VVA++ V+R +E + K GA S+ LV+KL Sbjct: 8 LGLVGLAVMGQNLILNAADHGFTVVAYNRTQSKVDRFLANEAKGKSIIGATSIEDLVAKL 67 Query: 58 GVPR-IAWIMVPHAFVDSVIEELAPLLSKGDIIIEAGNSQYKESIRRHGELAQQGIYFLD 116 PR I ++ A VD++I+EL P L KGDIII+ GNS + ++ RR+ EL +QGI F+ Sbjct: 68 KKPRKIMLLIKAGAPVDTLIKELVPHLDKGDIIIDGGNSHFPDTNRRYEELTKQGILFVG 127 Query: 117 AGTSGGMEGARNGACYMVGGDEEAWAVAEPLFRDTAVQDG----YLFAGKPGSGHFLKMV 172 +G SGG +GAR G M GG EAW + +F+ A + + G GSGH++KMV Sbjct: 128 SGVSGGEDGARFGPSLMPGGSAEAWPHIKNIFQSIAAKSNGEPCCEWVGPAGSGHYVKMV 187 Query: 173 HNGIEYGMMAAIGEGFEVLEKSG--FDYNFEQVARVWNNGSVIRSWLMELVERAFSKDAK 230 HNGIEYG M I E ++++++ G D +V WN G V+ S+L+E+ K Sbjct: 188 HNGIEYGDMQLICEAYDIMKRIGRFTDKEISEVFDKWNTG-VLDSFLIEITRDIL----K 242 Query: 231 LDEIKG------VMHSSGE---GRWTLETAFDLQAATPVIAMALLMRYRSLETD 275 D++ G +M ++G+ G+WT A DL +I A+ R S D Sbjct: 243 FDDVDGKPLVEKIMDTAGQKGTGKWTAINALDLGMPVTLIGEAVFARCLSAIKD 296 >YHR183w [G] COG0362 6-phosphogluconate dehydrogenase Length = 489 Score = 162 bits (409), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 117/306 (38%), Positives = 168/306 (54%), Gaps = 38/306 (12%) Query: 4 GLIGLGKMGANLGRNLLDHGHEVVAFDVNREAV-----SELESKGAAGADSLAGLVSKLG 58 GLIGL MG NL N DHG V A++ + V +E + K GA S+ +SKL Sbjct: 6 GLIGLAVMGQNLILNAADHGFTVCAYNRTQSKVDHFLANEAKGKSIIGATSIEDFISKLK 65 Query: 59 VPRIAWIMVPH-AFVDSVIEELAPLLSKGDIIIEAGNSQYKESIRRHGELAQQGIYFLDA 117 PR ++V A VD++I ++ PLL KGDIII+ GNS + +S RR+ EL ++GI F+ + Sbjct: 66 RPRKVMLLVKAGAPVDALINQIVPLLEKGDIIIDGGNSHFPDSNRRYEELKKKGILFVGS 125 Query: 118 GTSGGMEGARNGACYMVGGDEEAWAVAEPLFRD-TAVQDG---YLFAGKPGSGHFLKMVH 173 G SGG EGAR G M GG EEAW + +F+ +A DG + G G+GH++KMVH Sbjct: 126 GVSGGEEGARYGPSLMPGGSEEAWPHIKNIFQSISAKSDGEPCCEWVGPAGAGHYVKMVH 185 Query: 174 NGIEYGMMAAIGEGFEVLEKSG--FDYNFEQVARVWNNGSVIRSWLMELVERAFSKDAKL 231 NGIEYG M I E ++++++ G D V WNNG V+ S+L+E+ K Sbjct: 186 NGIEYGDMQLICEAYDIMKRLGGFTDKEISDVFAKWNNG-VLDSFLVEITRDIL----KF 240 Query: 232 DEIKG------VMHSSGE---GRWTLETAFDLQAATPVIAMALLMRYRSLETDTFTGKVV 282 D++ G +M ++G+ G+WT A DL +I A+ R + Sbjct: 241 DDVDGKPLVEKIMDTAGQKGTGKWTAINALDLGMPVTLIGEAVFAR------------CL 288 Query: 283 AALRNE 288 +AL+NE Sbjct: 289 SALKNE 294 >BS_gntZ [G] COG0362 6-phosphogluconate dehydrogenase Length = 468 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 101/287 (35%), Positives = 152/287 (52%), Gaps = 20/287 (6%) Query: 3 VGLIGLGKMGANLGRNLLDHGHEVVAFDVNR----EAVSELESKGAAGADSLAGLVSKLG 58 +G+IGLG MG+N+ N+ + G V ++ R + + +L+ + + L V L Sbjct: 5 IGVIGLGVMGSNIALNMANKGENVAVYNYTRDLTDQLIQKLDGQSLSPYYELEDFVQSLE 64 Query: 59 VPRIAWIMVPH-AFVDSVIEELAPLLSKGDIIIEAGNSQYKESIRRHGELAQQGIYFLDA 117 PR ++MV VDSVI+ L PLL +GD+I++ GNS Y+++ RR+ EL ++GI +L Sbjct: 65 KPRKIFLMVTAGKPVDSVIQSLKPLLEEGDVIMDGGNSHYEDTERRYDELKEKGIGYLGV 124 Query: 118 GTSGGMEGARNGACYMVGGDEEAWAVAEPLFRDTAVQDG----YLFAGKPGSGHFLKMVH 173 G SGG GA G M GGD + + A P+ A Q G ++ G G+GHF KMVH Sbjct: 125 GISGGEVGALTGPSIMPGGDRDVYEKAAPILTKIAAQVGDDPCCVYIGPKGAGHFTKMVH 184 Query: 174 NGIEYGMMAAIGEGFEVLEKSGFDYNFEQVARV---WNNGSVIRSWLMELV-ERAFSKDA 229 NGIEY M I E + L ++ +++A + WN G ++S+L+E+ E KD Sbjct: 185 NGIEYADMQLIAEAYTFLRET-LRLPLDEIASIFETWNQGE-LKSYLIEITAEILRKKDE 242 Query: 230 K-----LDEIKGVMHSSGEGRWTLETAFDLQAATPVIAMALLMRYRS 271 K +D I G G+WT A D + +I +L RY S Sbjct: 243 KTGQPLIDVILDKTGQKGTGKWTSMQAIDNGIPSTIITESLFARYLS 289 >STM0438 [R] COG0790 FOG: TPR repeat, SEL1 subfamily Length = 673 Score = 144 bits (363), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 20/294 (6%), Positives = 49/294 (16%), Gaps = 19/294 (6%) Query: 3 VGLIGLGKMGANLGRNLLDHGHEVVAFDVNREAVSELESKGAAGAD-----SLAGLVSKL 57 G + L G N+ A A + Sbjct: 5 PGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMK 64 Query: 58 GVPRIAWIMVPH-AFVDSVIEELAPLLSKGDIIIEAGNSQYKESIRRHGELAQQGIYFLD 116 + S L + K + + + + + Sbjct: 65 ERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGL 124 Query: 117 AGTSGGMEGARNGACYMVGGDEEAWAVAEPLFRDTA--VQDG--YLFAGKPGSGHFLKMV 172 + D + + A + L+ + + L Sbjct: 125 IEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACNVLDFD 184 Query: 173 HNGIEYGMMAAIGEGFEVLE--KSGFDYNFEQVARVWNNGSVIRSWLMELVERAFSKDAK 230 + ++ E K + E + + L+ R Sbjct: 185 DLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYE-LVKLLVGSRARFTVVGDD 243 Query: 231 ------LDEIKGVMHSSGEGRWTLETAFDLQAATPVIAMALLMRYRSLETDTFT 278 + + L+ L + + Sbjct: 244 DQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHV 297 Score = 49.7 bits (117), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 16/174 (9%), Positives = 30/174 (17%), Gaps = 34/174 (19%) Query: 148 FRDTAVQDGYLF----AGKPGSGHFLKMVHNGIEYGMMAAIGEGF----EVLEKSGFDYN 199 + V + LF G + E I F + + + Sbjct: 292 ANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRG 351 Query: 200 FEQVARVWNNGSVIRSWLMELVERAFSKD---------------AKLDEIKGVMHSSGEG 244 Q R +F D + ++ + Sbjct: 352 NHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPKR 411 Query: 245 RWTLETAFDLQAATPVIAMALLMRYRSLETDTFTGKVVAALRNEFGGHAVETKD 298 + + + FT L G E Sbjct: 412 ----------EIGPATLKKL-GEWAMTRNKSMFTASFDMGLSQTLSGRGYEALT 454 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,322,351 Number of extensions: 1036560 Number of successful extensions: 3934 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 3851 Number of HSP's successfully gapped: 57 Length of query: 298 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 192 Effective length of database: 26,153,027 Effective search space: 5021381184 Effective search space used: 5021381184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)