BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0020.p (451 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic ... 75 2e-13 PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic... 70 7e-12 SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmi... 60 6e-09 APE0035 [G] COG1653 ABC-type sugar transport system, periplasmic... 58 3e-08 CAC0429 [G] COG1653 ABC-type sugar transport system, periplasmic... 56 9e-08 >SP1897 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 419 Score = 75.1 bits (183), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 82/329 (24%), Positives = 144/329 (43%), Gaps = 42/329 (12%) Query: 70 KKAIAATVEA----FMKANPNVKIKDISETTSSAYLDWLKTKDAVGEFPDLVEMR----D 121 KK + T+E F K NP +K+K ++ + + LKT+ G+ PD+V + + Sbjct: 42 KKEMTKTLEEITRDFEKENPKIKVKVVNVPNAG---EVLKTRVLAGDVPDVVNIYPQSIE 98 Query: 122 TEVFADAGKIVELPS-DLLELFK----DPPQVNGKVWNAPMSVNVPQGIIYSKKAYADAG 176 + +A AG +L + D L+ K + VN KV+N P + N GI Y+K + + G Sbjct: 99 LQEWAKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANA-YGIYYNKDKFEELG 157 Query: 177 ITGLPKTYDEFIAIQEKLKTSGITPIVVGGKDIFHMGFWVNKFLIDEVYTKDPDWNSKRT 236 + +P+T+DEF + + + G TP + G D + + + +++ K Sbjct: 158 LK-VPETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGY------NQLAFATATGGGKEA 210 Query: 237 AKTVSFTDPNVVQAMTD--FKD--------LFTNYVDKGWLSTGDNQTASILVSGKAAQL 286 + + ++ PN ++ ++D KD K W G G Sbjct: 211 NQYLRYSQPNAIK-LSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMT 269 Query: 287 FSGTWMFSQIQEADPNFEFGFYAVPDRE-GKVNVIGLPSPAGWSLSAEAAKDADKTQAIK 345 +G+W + I E PNF+ G + +P +E G+ +G A WS+SA K + A Sbjct: 270 PNGSWAITAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLA-WSISA-TTKHPKEANA-- 325 Query: 346 DFIRFFFAPEQYASYLASINAIPSTKENV 374 F+ + PE Y ++ P+ E V Sbjct: 326 -FVEYMTRPEVMQKYY-DVDGSPTAIEGV 352 >PAE1324 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 437 Score = 70.1 bits (170), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 65/258 (25%), Positives = 114/258 (44%), Gaps = 36/258 (13%) Query: 76 TVEAFMKANPNVKIKDIS---ETTSSAYLDWLKTKDA--------VGEFPDLVEMRDTEV 124 ++ + K NPNV+ + ++ E + L+ K A G F +VEM Sbjct: 82 VLDEYQKINPNVEFEYVTRRAEEIAQILPTQLEAKQAPADVVITSFGWF--VVEM----- 134 Query: 125 FADAGKIVEL-----PSDLLELFKDPPQVNGKVWNAPMSVNVPQGIIYSKKAYADAGITG 179 A G +V+L P++ + DP GK+W P ++ + G Y K + G++ Sbjct: 135 -AKRGHVVDLTNVINPNEYVTGIFDPVTYEGKIWATPFTIVLKPGFWYRKSFFQQHGLSE 193 Query: 180 LPKTYDEFIAIQEKLKTSGITPIVVGGKDIFHMGFWVNKFLIDEVYTKDPDWNSKRTAKT 239 P+T+DEF+ + ++LK G+TPI G + + F+I PD T K Sbjct: 194 -PQTWDEFLQLLKRLKEIGVTPIASGDGVGWPLSDVTEAFIIA---YAGPDVFRGLTEKK 249 Query: 240 VSFTDPNVVQAMTDFKDLFTNYVDKGWLSTGDNQTASI--LVSGKAAQLFSGTWMFSQIQ 297 V F DP + F+D + + +G+ + T ++ +G F GTW+ + Sbjct: 250 VKFDDPRINAV---FRDRISLLLREGYFGEPEEWTQAVEKWWNGTYGIYFMGTWILGMVD 306 Query: 298 EADPNFEFGFYAVPDREG 315 D + GF+ +P+ +G Sbjct: 307 NPD---DVGFFPMPETKG 321 >SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 451 Score = 60.5 bits (145), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 65/249 (26%), Positives = 107/249 (42%), Gaps = 33/249 (13%) Query: 104 LKTKDAVGEFPDL--VEMRDTEVFADAGKIVEL--------PSDLLELFKDPPQVNGKVW 153 L T A GE PD+ + D + + G + +L +D E V+GK++ Sbjct: 98 LATAFASGEGPDIFIISPGDFLRYYNGGVLKDLTPFIDDKAKADFPESVIANRMVDGKIF 157 Query: 154 NAPMSVNVPQGIIYSKKAYADAGIT--GLPKTYDEFIAIQEKLKTSGITPIVVGGKDIFH 211 PM V P + YS KA+ +AG+ +PKT++ + + +KL T ++ + ++ Sbjct: 158 GIPMEVE-PMAMYYSVKAFEEAGLNENDVPKTWEALLEVAKKLTTPERFGVMFETQPGYY 216 Query: 212 MGFWVNKFLIDEVYTKDPDWNSKRTAKTV----SFTDPNVVQAMTDFKDLFTNYV-DKGW 266 F FL W +T SF P VQA+ ++D + V + Sbjct: 217 QNFTWYPFL----------WQGGGEFQTADGKSSFDSPATVQALKFWQDAINSGVAPRQI 266 Query: 267 LSTGDNQTASILVSGKAAQLFSGTWMFSQIQEADPNFEFGFYAVPD-REGKVNVIGLPSP 325 + G + T + L SG A G W SQ+Q +F++G + +P GK +G Sbjct: 267 MGAGGHDTIANLASGYVAMQNVGIWGISQLQSNAKDFQYGVFRLPTPPNGKYVTVG---- 322 Query: 326 AGWSLSAEA 334 GW+ A A Sbjct: 323 GGWAFVANA 331 >APE0035 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 452 Score = 58.2 bits (139), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 69/261 (26%), Positives = 117/261 (44%), Gaps = 37/261 (14%) Query: 77 VEAFMKANPNVKIK-------DISETTSSAYLDWLKTKDAVGEFPD---LVEMRDTEVFA 126 +E FM+ NPNV IK +++ T S + + D V P +VEM A Sbjct: 94 LEKFMEENPNVTIKYVPMRAEEVARTLSVQFEAGVTPADVVIT-PWAWWIVEM------A 146 Query: 127 DAGKIVELPSDLLE-----LFKDPPQVNGKVWNAPMSVNVPQGIIYSKKAYADAGITGLP 181 G ++E+ + E D + N K+W P ++ + G Y K +A G++ P Sbjct: 147 QKGHVIEVTGLINEDEYVGGILDNVKWNNKLWGVPFTMWLKPGFWYKKSFFAKHGLSE-P 205 Query: 182 KTYDEFIAIQEKLK-TSGI-TPIVVGGKDIFHMGFWVNKFLIDEVYTKDPDWNSKRTAKT 239 +++EF+ + +++K GI PIV G + + F+I P+ K Sbjct: 206 NSWEEFLQLLDQIKGIEGIKNPIVSGDSVGWPLSDVTEHFIIA---FGGPELQYKLITGE 262 Query: 240 VSFTDPNVVQAMTDFKDLFTNYVDKGWLSTGDNQTASI--LVSGKAAQLFSGTWMFSQIQ 297 VSFTDP VV+ L +++G+ S T+++ + + A F GTW+ ++ Sbjct: 263 VSFTDPQVVEIFEKLAML----IEQGYFSEPIEWTSAVEKWWAEEYALYFMGTWITGMVE 318 Query: 298 EADPNFEFGFYAVPDREGKVN 318 DPN + F+ +P G V Sbjct: 319 --DPN-DLAFFPLPGARGVVG 336 >CAC0429 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 447 Score = 56.2 bits (134), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 85/355 (23%), Positives = 153/355 (43%), Gaps = 44/355 (12%) Query: 71 KAIAATVEAFMKANPNVKIKDISETTSSAYLDWLKTKDAVGEFPDLVEMRD--------- 121 +A+ VE F ++ ++K+K + L+ LK+ + GE PD+V++ D Sbjct: 49 EALNKMVEDFNNSHSDIKVKAEFQGKYDEELNKLKSAEKGGEAPDIVQVYDIGTRFMIDS 108 Query: 122 -----TEVFADAGK--IVELPSDLLELFKDPPQVNGKVWNAPMSVNVPQGIIYSKKAYAD 174 + F D K L +LL + VN K+++ P + + P + Y+K A+ + Sbjct: 109 KWAEPIQNFIDKDKYDTSSLEPNLLAYY----TVNNKLYSMPFNSSTPI-LYYNKSAFKE 163 Query: 175 AGI--TGLPKTYDEFIAIQEKL-KTSGITPIVVGGKDIFHMGFWVNKFLIDEVYTKDPDW 231 A + PKT+ E + L K + G + G+ ++L+ + + Sbjct: 164 ADLDPNKPPKTFSELERYSKALAKKDSSGNVTRYGFSMAIYGWLFEQYLVKQGKNYVNNG 223 Query: 232 NSKRTAKTVSFTDPN--VVQAMTDFKDLFTNYVDKGW---LSTGDNQTASILVSGKAAQL 286 N + +A T D N ++ + ++K L VD G+ L ++ T + ++GK A Sbjct: 224 NGRSSAATKVDFDSNGGGLKFLKEWKQL----VDSGYVGNLGRNEDDTENAFIAGKTAMY 279 Query: 287 FSGTW-MFSQIQEADPNFEFGFYAVPDREGKVNVIGLPSPAGWSLSAEAAKDADKTQAIK 345 T + + FE G +P +G + G S G S+ KDA+K +A Sbjct: 280 IESTADLKHDLNSIGGRFELGTAPLPSFDGTSD--GGVSIGGASMWILKNKDAEKQKAAF 337 Query: 346 DFIRFFFAPEQYASYLASINAIPSTK----ENVTYET---GEQMQTALD-LIADP 392 +FI++ + +Q A + P TK E++ E Q +TA+D L A P Sbjct: 338 EFIKYMVSSKQQAYWSTETGYFPVTKKAYDEDIMKENLKKSPQFKTAIDQLYASP 392 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.131 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,293,390 Number of extensions: 1337525 Number of successful extensions: 2490 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2486 Number of HSP's successfully gapped: 13 Length of query: 451 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 341 Effective length of database: 25,691,691 Effective search space: 8760866631 Effective search space used: 8760866631 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 117 (49.7 bits)