BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0022.p (354 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1511 [R] COG0673 Predicted dehydrogenases and related proteins 278 7e-75 AGl1005 [R] COG0673 Predicted dehydrogenases and related proteins 278 9e-75 lin2932 [R] COG0673 Predicted dehydrogenases and related proteins 89 1e-17 BS_yrbE [R] COG0673 Predicted dehydrogenases and related proteins 80 4e-15 STM0585 [P] COG4771 Outer membrane receptor for ferrienterocheli... 79 9e-15 >mll1511 [R] COG0673 Predicted dehydrogenases and related proteins Length = 343 Score = 278 bits (712), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 149/346 (43%), Positives = 205/346 (59%), Gaps = 21/346 (6%) Query: 7 VAVAGCGGMANEWISYAVRRPDVRIVALVDIRLDAAAAMAGKHGLTCPVF-TDIGEAIEQ 65 V + GCG M+ +W+ A + + IV LVD+ + A A A ++ L + T + ++Q Sbjct: 4 VVLVGCGAMSRQWLDAARQIDGLAIVGLVDLDAERAKARAHEYDLAGIIIGTSLDAVLDQ 63 Query: 66 TGANLVFDVTIPASHYRVASTALTHQCDVFSEKPLAETMEHCNAIVALAERTGKSHAVMQ 125 T + VFDV +PA+ VA +A H C + +EKPLA++ E+ AIV A + G+ HAV+Q Sbjct: 64 TRPDAVFDVVVPAARRAVALSAFAHNCHLLTEKPLADSPENARAIVEAARQAGRIHAVVQ 123 Query: 126 NRRYDPRIRSLRRLIEAGEIGRIGYIGAGFFLAPHFGGFRDAMESPLLLDMAIHTFDQAR 185 NRRY +R +RR +++G IG I A FF+APHFGGFR+ M LLLDMAIHTFD AR Sbjct: 124 NRRYVANVRRIRRFLDSGAIGNPTSIHADFFVAPHFGGFREEMAHVLLLDMAIHTFDAAR 183 Query: 186 FISGADPVTVYCQEFNPPGSWYAGNASAVCIFEMSDGSVFCYQGSWCAEGAPTSWEATWR 245 +++G +P VYCQE+ P SWY +SA +F++ G VF Y GSWCA+G TSWE +WR Sbjct: 184 YMAGGEPAGVYCQEWEPANSWYRQGSSASAVFDLGGGKVFTYAGSWCADGFRTSWEGSWR 243 Query: 246 IQGDKGTAIWDGEGMPYAEKVTDRSPDGEFIRDH----------DRVEGEAADLKETFHY 295 I ++G+ IWDG + AE V DG F + DRV G H Sbjct: 244 IVAERGSLIWDGHDLLTAEVVAT-GRDGLFAKTQPIDVPPLDPADRVGG---------HL 293 Query: 296 GCLEEMFQALAERRPAETDCRDNRYSMAMVFGALESARTGRKVELA 341 G + + A+ ET DN S+AMVFGA+ESA TGR+V +A Sbjct: 294 GIIRDFMHAIETGTEPETHGADNIKSLAMVFGAIESAETGRRVAIA 339 >AGl1005 [R] COG0673 Predicted dehydrogenases and related proteins Length = 134 Score = 278 bits (711), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 0/344 (0%), Positives = 0/344 (0%), Gaps = 10/344 (2%) Query: 5 YRVAVAGCGGMANEWISYAVRRPDV----RIVALVDIRLDAAAAMAGKHGLTCPVF-TDI 59 + + + + + + A + F Sbjct: 11 ITFSSPAFAASCEKNFTVSGVPMVTAVSYKSFQELPKAKAPAVLQKLAQAVAAEGFSGIQ 70 Query: 60 GEAIEQTGANLVFDVTIPASHYRVASTALTHQCDVFSEKPLAETMEHCNAIVALAERTGK 119 + + + + V Sbjct: 71 INKALSSIDAHQETSGSGRIQTLRVVARQKGAAVRIDAVFNIQAGQIADKDVIRKGICDI 130 Query: 120 SHAVMQNRRYDPRIRSLRRLIEAGEIGRIGYIGAGFFLAPHFGGFRDAMESPLLLDMAIH 179 Sbjct: 131 190 Query: 180 TFDQARFISGADPVTVYCQEFNPPGSWYAGNASAVCIFEMSDGSVFCYQGSWCAEGAPTS 239 Sbjct: 191 250 Query: 240 WEATWRIQGDKGTAIWDGEGMPYAEKVTDRSPDGEFIRDHDRVE-GEAADLKETF-HYGC 297 Sbjct: 251 --- 307 Query: 298 LEEMFQALAERRPAETDCRDNRYSMAMVFGALESARTGRKVELA 341 Sbjct: 308 351 >lin2932 [R] COG0673 Predicted dehydrogenases and related proteins Length = 333 Score = 89.0 bits (219), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 68/258 (26%), Positives = 107/258 (41%), Gaps = 4/258 (1%) Query: 1 MTAQYRVAVAGCGGMANEWISYAVRRPDVRIVALVDIRLDAAAAMAGKHGLTCPVFTDIG 60 MT Q +V V G G M + P V +VA+ + + A MA K C + D Sbjct: 1 MTKQIKVGVIGVGQMGQYHAEIYQKLPQVELVAICEFNDERRAEMAEK--FQCKAYKDYH 58 Query: 61 EAIEQTGANLVFDVTIPASHYRVASTALTHQCDVFSEKPLAETMEHCNAIVALAERTGKS 120 E + T V V H A+ H+ + EKPLA+ +E A+ L + K Sbjct: 59 ELLANTEIEAVIIVLPDNMHRDCVEEAVKHKKHILLEKPLAKELEDGKAMYELTKDYDKV 118 Query: 121 HAVMQNRRYDPRIRSLRRLIEAGEIGRIGYIGAGFFLAPHFGGFRDAMESPLLLDMAIHT 180 V R+DPR +++ ++ GE+G I ++ +P G R S L + + IH Sbjct: 119 FTVGFLLRFDPRFNMIKQRLDNGELGDIIHLYCRRN-SPITGPKRYIGASDLSMHVMIHD 177 Query: 181 FDQARFISGADPVTVYCQEFNPPGSWYAGNASAVCIFEMSDGSVFCYQGSWCA-EGAPTS 239 D + ++PV V + + Y N I +G++ C + W E +PT Sbjct: 178 IDYINWYMDSEPVKVIAKSRSVLLKEYGMNDVIYAIVTYENGAIACLEACWVLPENSPTI 237 Query: 240 WEATWRIQGDKGTAIWDG 257 + + G KG A D Sbjct: 238 IDDKLELVGTKGVAYVDS 255 >BS_yrbE [R] COG0673 Predicted dehydrogenases and related proteins Length = 260 Score = 80.5 bits (197), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 22/271 (8%), Positives = 52/271 (19%), Gaps = 29/271 (10%) Query: 1 MTAQYRVAVAGCGGMANEWISYAVRRPDVRIVALVDIRLDAAAAMAGKHGLTCPVFTDIG 60 M ++ G A +V + R A + + + + I Sbjct: 1 MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLLVRQLYNVGV-LSMLII 59 Query: 61 EAIEQTGANLVFDVTIPASHYRVASTALTHQCDVFSEKPLAETMEHCNAIVALAERTGKS 120 ++ A T+L + + L + Sbjct: 60 VVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPVVAALLFAGRAGSALTAE 119 Query: 121 HAVMQNRRYDPRIRSLRRLIEAGEIGRIGYIGAGFFLAPHFGGFRDAMESP--------- 171 +M+ + + I + G Sbjct: 120 IGLMRATEQLSSMEMMAVDPLRRVIS-----------PRFWAGVISLPLLTVIFVAVGIW 168 Query: 172 --LLLDMAIHTFDQARFISGADPVTVYCQ---EFNPPGSWYAGNASAVCIFEMSDGSVFC 226 L+ ++ D F S + +A + + +F D Sbjct: 169 GGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISLFNGYDAIPTS 228 Query: 227 YQGSWCAEGAPTSWEATWRIQGDKGTAIWDG 257 S + + + Sbjct: 229 AGIS---RATTRTVVHSSLAVLGLDFVLTAL 256 >STM0585 [P] COG4771 Outer membrane receptor for ferrienterochelin and colicins Length = 751 Score = 79.3 bits (194), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 18/363 (4%), Positives = 50/363 (13%), Gaps = 47/363 (12%) Query: 4 QYRVAVAGCGGMA-NEWISYAVRRPD----VRIVALVDIRLDAAAAMAGKHGLTCP-VFT 57 ++ + V D + I + AA G++ Sbjct: 2 NKKIHSLTLLVNLGIYGATLPVMAEDKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADE 61 Query: 58 DIGEAIEQTGANLVFDVTIPASHYRVASTALTHQCDVFSEKPLAETMEHCNAIVALAERT 117 + + ++ + S + + E + R Sbjct: 62 IRKNPPARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRN 121 Query: 118 GKSHAVMQNRRYDPRIRSLRRLIEAGEIGRIGYIGAGFFLAPHFGGFRDA-------MES 170 R + + G A + G Sbjct: 122 SVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGGSEWHG 181 Query: 171 PLLLDMAIHTFDQARFISGADPVTVYCQEFNPPGSWYAGNASAVCIFEMSDGSVFCYQGS 230 + + Y + + Sbjct: 182 SWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERTG 241 Query: 231 WCAEGAPTSWEATWRIQGDKGTAIWDG--------------EGMPYAEKVTDRSPDGEFI 276 + I D + Sbjct: 242 --SYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYA-----------G 288 Query: 277 RDHDRVEGEAADLKETFHYGCLEEMFQALAERRPAETDCRDNRYSMAMVFGALESARTGR 336 + + + L + + T + Sbjct: 289 DTQNTNTNQL-------VKDNYGKETNRLYRQNYSLTWNGGWNNGVTTSNWVQYEHTRNS 341 Query: 337 KVE 339 ++ Sbjct: 342 RMP 344 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.136 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,386,467 Number of extensions: 1154836 Number of successful extensions: 1898 Number of sequences better than 1.0e-05: 73 Number of HSP's gapped: 1864 Number of HSP's successfully gapped: 73 Length of query: 354 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 247 Effective length of database: 26,037,693 Effective search space: 6431310171 Effective search space used: 6431310171 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)