BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0010.p (417 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs2523 [E] COG1760 L-serine deaminase 273 4e-73 sdaA [E] COG1760 L-serine deaminase 273 5e-73 ZsdaA [E] COG1760 L-serine deaminase 273 5e-73 sdaB [E] COG1760 L-serine deaminase 266 4e-71 >ECs2523 [E] COG1760 L-serine deaminase Length = 454 Score = 273 bits (697), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 180/460 (39%), Positives = 238/460 (51%), Gaps = 65/460 (14%) Query: 16 MRSLTELYKIGSGPSSSHTMGPEKAARIF------KAENEDADQFKALIYGSLAKTGKGH 69 M SL +++K+G GPSSSHT+GP KA + F K + + +YGSL+ TGKGH Sbjct: 1 MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH 60 Query: 70 MTDKAIIRALS---PASAEVEFVP-------------------QPDFV-----------L 96 TD AII L+ PA+ +++ +P + DF L Sbjct: 61 HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL 120 Query: 97 P-HPNTMDLFAYQDGQQTNSMRAVSIGGGDIVIEGREETHGPDV-------YPENSFAEI 148 P H N M + AY + S SIGGG IV EE G D YP S E+ Sbjct: 121 PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIV---DEEHFGQDTANEVSVPYPFKSATEL 177 Query: 149 SAFCKANHIRLSDYVEQRE-----GNQIWEFLQGIWEAMKRSIDEGLSVTGILEGGLHVE 203 A+C LS Q E +I E+ +W+ M+ ID G++ G+L G L V Sbjct: 178 LAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVP 237 Query: 204 RKAKYLYHQRDMDESPETREN------RIVSAYAFAVNEQNAAAGTVVTAPTCGASGVVP 257 R+A L R M S + N V+ +A AVNE+NAA G VVTAPT GA G+VP Sbjct: 238 RRASAL---RRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVP 294 Query: 258 ASLRYMQEKLQ-VPDERILRALAVGGLIGNLVKQNASISGAQCGCQAEVXXXXXXXXXXX 316 A L Y ++ V + R G IG L K NASISGA+ GCQ EV Sbjct: 295 AVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGL 354 Query: 317 XXXXXXXIDQIEYAAEVAMEHHLGLTCDPINGLVQIPCIERNAVGAMRAINALSLAKFLS 376 +Q+ AAE+ MEH+LGLTCDP+ G VQ+PCIERNA+ +++AINA +A + Sbjct: 355 AELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRT 414 Query: 377 GTRKISFDLVVQTMYETGQDMNSSYRETSEGGLAKLYKID 416 ++S D V++TMYETG+DMN+ YRETS GGLA + D Sbjct: 415 SAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD 454 >sdaA [E] COG1760 L-serine deaminase Length = 454 Score = 273 bits (697), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 180/460 (39%), Positives = 238/460 (51%), Gaps = 65/460 (14%) Query: 16 MRSLTELYKIGSGPSSSHTMGPEKAARIF------KAENEDADQFKALIYGSLAKTGKGH 69 M SL +++K+G GPSSSHT+GP KA + F K + + +YGSL+ TGKGH Sbjct: 1 MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH 60 Query: 70 MTDKAIIRALS---PASAEVEFVP-------------------QPDFV-----------L 96 TD AII L+ PA+ +++ +P + DF L Sbjct: 61 HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL 120 Query: 97 P-HPNTMDLFAYQDGQQTNSMRAVSIGGGDIVIEGREETHGPDV-------YPENSFAEI 148 P H N M + AY + S SIGGG IV EE G D YP S E+ Sbjct: 121 PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIV---DEEHFGQDAANEVSVPYPFKSATEL 177 Query: 149 SAFCKANHIRLSDYVEQRE-----GNQIWEFLQGIWEAMKRSIDEGLSVTGILEGGLHVE 203 A+C LS Q E +I E+ +W+ M+ ID G++ G+L G L V Sbjct: 178 LAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVP 237 Query: 204 RKAKYLYHQRDMDESPETREN------RIVSAYAFAVNEQNAAAGTVVTAPTCGASGVVP 257 R+A L R M S + N V+ +A AVNE+NAA G VVTAPT GA G+VP Sbjct: 238 RRASAL---RRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVP 294 Query: 258 ASLRYMQEKLQ-VPDERILRALAVGGLIGNLVKQNASISGAQCGCQAEVXXXXXXXXXXX 316 A L Y ++ V + R G IG L K NASISGA+ GCQ EV Sbjct: 295 AVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGL 354 Query: 317 XXXXXXXIDQIEYAAEVAMEHHLGLTCDPINGLVQIPCIERNAVGAMRAINALSLAKFLS 376 +Q+ AAE+ MEH+LGLTCDP+ G VQ+PCIERNA+ +++AINA +A + Sbjct: 355 AELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRT 414 Query: 377 GTRKISFDLVVQTMYETGQDMNSSYRETSEGGLAKLYKID 416 ++S D V++TMYETG+DMN+ YRETS GGLA + D Sbjct: 415 SAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD 454 >ZsdaA [E] COG1760 L-serine deaminase Length = 454 Score = 273 bits (697), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 180/460 (39%), Positives = 238/460 (51%), Gaps = 65/460 (14%) Query: 16 MRSLTELYKIGSGPSSSHTMGPEKAARIF------KAENEDADQFKALIYGSLAKTGKGH 69 M SL +++K+G GPSSSHT+GP KA + F K + + +YGSL+ TGKGH Sbjct: 1 MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH 60 Query: 70 MTDKAIIRALS---PASAEVEFVP-------------------QPDFV-----------L 96 TD AII L+ PA+ +++ +P + DF L Sbjct: 61 HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL 120 Query: 97 P-HPNTMDLFAYQDGQQTNSMRAVSIGGGDIVIEGREETHGPDV-------YPENSFAEI 148 P H N M + AY + S SIGGG IV EE G D YP S E+ Sbjct: 121 PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIV---DEEHFGQDAANEVSVPYPFKSATEL 177 Query: 149 SAFCKANHIRLSDYVEQRE-----GNQIWEFLQGIWEAMKRSIDEGLSVTGILEGGLHVE 203 A+C LS Q E +I E+ +W+ M+ ID G++ G+L G L V Sbjct: 178 LAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVP 237 Query: 204 RKAKYLYHQRDMDESPETREN------RIVSAYAFAVNEQNAAAGTVVTAPTCGASGVVP 257 R+A L R M S + N V+ +A AVNE+NAA G VVTAPT GA G+VP Sbjct: 238 RRASAL---RRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVP 294 Query: 258 ASLRYMQEKLQ-VPDERILRALAVGGLIGNLVKQNASISGAQCGCQAEVXXXXXXXXXXX 316 A L Y ++ V + R G IG L K NASISGA+ GCQ EV Sbjct: 295 AVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGL 354 Query: 317 XXXXXXXIDQIEYAAEVAMEHHLGLTCDPINGLVQIPCIERNAVGAMRAINALSLAKFLS 376 +Q+ AAE+ MEH+LGLTCDP+ G VQ+PCIERNA+ +++AINA +A + Sbjct: 355 AELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRT 414 Query: 377 GTRKISFDLVVQTMYETGQDMNSSYRETSEGGLAKLYKID 416 ++S D V++TMYETG+DMN+ YRETS GGLA + D Sbjct: 415 SAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD 454 Score = 272 bits (696), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 180/460 (39%), Positives = 238/460 (51%), Gaps = 65/460 (14%) Query: 16 MRSLTELYKIGSGPSSSHTMGPEKAARIF------KAENEDADQFKALIYGSLAKTGKGH 69 M SL +++K+G GPSSSHT+GP KA + F K + + +YGSL+ TGKGH Sbjct: 1 MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH 60 Query: 70 MTDKAIIRALS---PASAEVEFVP-------------------QPDFV-----------L 96 TD AII L+ PA+ +++ +P + DF L Sbjct: 61 HTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGRHEVDFPRDNGMRFHNGNL 120 Query: 97 P-HPNTMDLFAYQDGQQTNSMRAVSIGGGDIVIEGREETHGPDV-------YPENSFAEI 148 P H N M + AY + S SIGGG IV EE G D YP S E+ Sbjct: 121 PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIV---DEEHFGQDAANEVSVPYPFKSATEL 177 Query: 149 SAFCKANHIRLSDYVEQRE-----GNQIWEFLQGIWEAMKRSIDEGLSVTGILEGGLHVE 203 A+C LS Q E +I E+ +W+ M+ ID G++ G+L G L V Sbjct: 178 LAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVP 237 Query: 204 RKAKYLYHQRDMDESPETREN------RIVSAYAFAVNEQNAAAGTVVTAPTCGASGVVP 257 R+A L R M S + N V+ +A AVNE+NAA G VVTAPT GA G+VP Sbjct: 238 RRASAL---RRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVP 294 Query: 258 ASLRYMQEKLQ-VPDERILRALAVGGLIGNLVKQNASISGAQCGCQAEVXXXXXXXXXXX 316 A L Y ++ V + R G IG L K NASISGA+ GCQ EV Sbjct: 295 AVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGL 354 Query: 317 XXXXXXXIDQIEYAAEVAMEHHLGLTCDPINGLVQIPCIERNAVGAMRAINALSLAKFLS 376 +Q+ AAE+ MEH+LGLTCDP+ G VQ+PCIERNA+ +++AINA +A + Sbjct: 355 AELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRT 414 Query: 377 GTRKISFDLVVQTMYETGQDMNSSYRETSEGGLAKLYKID 416 ++S D V++TMYETG+DMN+ YRETS GGLA + D Sbjct: 415 SAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD 454 >sdaB [E] COG1760 L-serine deaminase Length = 455 Score = 266 bits (680), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 171/448 (38%), Positives = 240/448 (53%), Gaps = 53/448 (11%) Query: 16 MRSLTELYKIGSGPSSSHTMGPEKAARIFK----AEN--EDADQFKALIYGSLAKTGKGH 69 M S+ +++KIG GPSSSHT+GP KA + F A N +D + +YGSL+ TGKGH Sbjct: 1 MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTRVVVDVYGSLSLTGKGH 60 Query: 70 MTDKAIIRALS---PASAEVEFVP-----------------QPDFVLPHPNTMDLFAYQD 109 TD AII L+ P + +++ +P Q + P M+ A Sbjct: 61 HTDIAIIMGLAGNLPDTVDIDSIPSFIQDVNTHGRLMLANGQHEVEFPVDQCMNFHADNL 120 Query: 110 GQQTNSMRAV--------------SIGGGDIVIE---GREETHGPDV-YPENSFAEISAF 151 N MR SIGGG IV E G++++ +V YP +S A++ Sbjct: 121 SLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQDSAPVEVPYPYSSAADLQKH 180 Query: 152 CKANHIRLSDYVEQRE-----GNQIWEFLQGIWEAMKRSIDEGLSVTGILEGGLHVERKA 206 C+ + LS + + E ++ + L +WE M+ I+ G+S G+L G L V R+A Sbjct: 181 CQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRGGIERGISTEGVLPGKLRVPRRA 240 Query: 207 KYLYHQRDMDESPETRENRIV---SAYAFAVNEQNAAAGTVVTAPTCGASGVVPASLRYM 263 L + T +V + +A AVNE+NAA G VVTAPT GA G++PA L Y Sbjct: 241 AALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENAAGGRVVTAPTNGACGIIPAVLAYY 300 Query: 264 QEKL-QVPDERILRALAVGGLIGNLVKQNASISGAQCGCQAEVXXXXXXXXXXXXXXXXX 322 + + +V + R L V IG+L K NASISGA+ GCQ EV Sbjct: 301 DKFIREVNANSLARYLLVASAIGSLYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGA 360 Query: 323 XIDQIEYAAEVAMEHHLGLTCDPINGLVQIPCIERNAVGAMRAINALSLAKFLSGTRKIS 382 Q+ AAE+AMEH+LGLTCDP+ G VQ+PCIERNA+ A++A+NA +A + ++ Sbjct: 361 SPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPCIERNAIAAVKAVNAARMALRRTSEPRVC 420 Query: 383 FDLVVQTMYETGQDMNSSYRETSEGGLA 410 D V++TMYETG+DMN+ YRETS GGLA Sbjct: 421 LDKVIETMYETGKDMNAKYRETSRGGLA 448 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,133,283 Number of extensions: 1065644 Number of successful extensions: 3378 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 3241 Number of HSP's successfully gapped: 49 Length of query: 417 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 308 Effective length of database: 25,807,025 Effective search space: 7948563700 Effective search space used: 7948563700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)