BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0008.p (279 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0435 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase 86 7e-17 AF0963 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase 83 6e-16 FN1020 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase 82 1e-15 DR0184 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase 81 2e-15 CAC2712 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase 81 2e-15 >AF0435 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Length = 346 Score = 85.9 bits (211), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 14/218 (6%), Positives = 27/218 (12%), Gaps = 17/218 (7%) Query: 33 EVRLHTGGGPYKHN-WEAHTAWSHAWSDIGRDPENEVMIISGTGDKWVIGDPEVWNTKFM 91 EV G A + + + + + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG-------L 64 Query: 92 DWPKQKKLEQYHESLRLLENL---IFCIDIPTIGAVNG--PGTHCELATLCDITICTEDA 146 H S L G L D + Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 147 DFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKNINGQTALDLGIVSEVLPREQL 206 P + + A T + V Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 207 LPRAWELAEMIMQ----APRSTRHLSHSIISRPWKQAL 240 + + + + Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEV 222 >AF0963 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Length = 346 Score = 82.8 bits (203), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 15/175 (8%), Positives = 36/175 (20%), Gaps = 6/175 (3%) Query: 68 VMIISGTGDKWVIGDPEVWNTKFMDWPKQKKLEQYHESLRLLENLIFCIDIPTIGAVNG- 126 V + + + + S + + + Sbjct: 49 VAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSR----NNDKGIRPDQA 104 Query: 127 -PGTHCELATLCDITICTEDADFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKN 185 + A + D + P L + Sbjct: 105 LDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDAL 164 Query: 186 INGQTALDLGIVSEVLPREQLLPRAWELAEMIMQAPRSTRHLSHSIISRPWKQAL 240 Q A + + + R LA+ T +S+++R ++ L Sbjct: 165 AEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGL 219 >FN1020 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Length = 346 Score = 82.0 bits (201), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 12/168 (7%), Positives = 28/168 (16%), Gaps = 8/168 (4%) Query: 68 VMIISGTGDKWVIGDPEVWNTKFMDWPKQKKLE-QYHESLRLLENLIFCIDIPTIGAVNG 126 + K E S + + + Sbjct: 48 DVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSR----NNDKGIRPDQ 103 Query: 127 --PGTHCELATLCDITICTEDADFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGK 184 + A + D + P L + Sbjct: 104 ALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDA 163 Query: 185 NINGQTALDLGIVSEVLPREQLLPRAWELAEMIMQ-APRSTRHLSHSI 231 Q A + + + R LA+ + ++ + Sbjct: 164 LAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLL 211 >DR0184 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Length = 346 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 10/200 (5%), Positives = 31/200 (15%), Gaps = 7/200 (3%) Query: 30 GIIEVRLHTGGGPYKHNWEAHTAWSHAWSDIGRDPENEVMIISGTGDKWVIG-DPEVWNT 88 + + P E ++ T + Sbjct: 23 FGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKL 82 Query: 89 KFMDWPKQKKLEQYHESLRLLENLIFCIDIPTIGAVNGP--GTHCELATLCDITICTEDA 146 ++ + + + L Sbjct: 83 IIASKVSGPSR--NNDKGIRPDQALDR-KNIREALHDSLKRLQTDYLDLYQVHWPQRPTN 139 Query: 147 DFFDPHYLGGTPPGDGMLLTLQNMIGFKKAAYYAYTGKNINGQTALDLGIVSEVLPREQL 206 F Y LL + + + A N + + + Sbjct: 140 CFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLP 199 Query: 207 LPRAWELA-EMIMQAPRSTR 225 + ++ ++ Sbjct: 200 RIVTIQNPYSLLNRSFEVGL 219 >CAC2712 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Length = 346 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 12/195 (6%), Positives = 33/195 (16%), Gaps = 13/195 (6%) Query: 46 NWEAHTAWSHAWSDIGRDPENEVMIISGTGDKWVIGDPEVWNTKFMDWPKQKKL----EQ 101 +AH +A + + M + ++KL + Sbjct: 29 EADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKV 88 Query: 102 YHESLRLLENLIFCIDIPTIGAVNG--PGTHCELATLCDITICTEDADFFDPHYLGGTPP 159 S + +L + D Sbjct: 89 SGPSRNNDKG-------IRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCF 141 Query: 160 GDGMLLTLQNMIGFKKAAYYAYTGKNINGQTALDLGIVSEVLPREQLLPRAWELAEMIMQ 219 G + + +G+ +E + ++ Sbjct: 142 GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRI 201 Query: 220 APRSTRHLSHSIISR 234 + + Sbjct: 202 VTIQNPYSLLNRSFE 216 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,306,046 Number of extensions: 996982 Number of successful extensions: 1468 Number of sequences better than 1.0e-05: 62 Number of HSP's gapped: 1465 Number of HSP's successfully gapped: 62 Length of query: 279 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 174 Effective length of database: 26,268,361 Effective search space: 4570694814 Effective search space used: 4570694814 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)