BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0007.p (405 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 267 2e-71 slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 262 6e-70 FN1063 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 258 1e-68 CAC1014 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 256 6e-68 PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 254 2e-67 >alr4934 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 359 Score = 267 bits (683), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 0/378 (0%), Positives = 0/378 (0%), Gaps = 12/378 (3%) Query: 15 LQDKMVMYRHHLHAVPELDLSLPRTTAYVKEVLESMGLK-PAPVGESGLMVTIGGQH-AG 72 L M L T + L+ + G+ + Sbjct: 23 LAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFF 82 Query: 73 KVILVRADMDGLPIQEETELSYASL-NGNMHACGHDMHTSMLLGAAEILKASEGQLRGTV 131 + + + + G + E Sbjct: 83 DARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYH 142 Query: 132 KLMFQPGEETLHGAKMMLENGILDNPKVDAAMMLHVLTGMPLPIGQFVVPEEGGAISASS 191 +L + T A Sbjct: 143 RLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGK----PGQRSIAASGGIL 198 Query: 192 DWFEIIIRGRGSHGAMPEAAVDPLNVAAHLHLALQGILSREISPIDNAVLTIGVMEGGTT 251 W + + + + + G T Sbjct: 199 LWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGT 258 Query: 252 NNVIPDTARLKGSVRTFNAALRDKVETRIREISAGIGDTFQAKVEVIYTRGCPEVKTDGG 311 DT ++ + R ++ + D Sbjct: 259 WVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKA 318 Query: 312 LNEQMRASIGNTFGDGSYIGITELVPGGKLMGSEDFAFVSQAVPSTTVFLNAGNAEEGYS 371 + + G + Sbjct: 319 GDPVILWGEGLPV--ERIAEM--- 373 Query: 372 YPVHHPKTMFSDEVLHRG 389 Sbjct: 374 391 >slr1653 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 262 bits (670), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 144/377 (38%), Positives = 212/377 (56%), Gaps = 11/377 (2%) Query: 15 LQDKMVMYRHHLHAVPELDLSLPRTTAYVKEVLESMGLKPAP-VGESGLMVTIGGQHAGK 73 L +++ +R H PEL T A++ E L + + P + ++G+M T+ G Sbjct: 35 LHGQLIQWRRQFHQYPELGFQEQLTAAHIAETLTKLEIPHTPGIAKTGIMATVDSGKPGP 94 Query: 74 VILVRADMDGLPIQEETELSYASLN-GNMHACGHDMHTSMLLGAAEILKASEGQLRGTVK 132 V+ +RADMD LP+ EE E+ Y SL+ G MHACGHD HT++ LG A+ L A RG VK Sbjct: 95 VLAIRADMDALPVTEENEVDYRSLHPGKMHACGHDGHTAIALGTAQYLAAHRDSFRGQVK 154 Query: 133 LMFQPGEETLHGAKMMLENGILDNPKVDAAMMLHVLTGMPLPIGQFVVPEEGGAISASSD 192 FQP EE GAK M+E G+L+NP VDA + LH+ LP+G V + G + A+ + Sbjct: 155 FFFQPAEEGPGGAKPMIEAGVLENPAVDAIVGLHLWND--LPVG--TVGIKPGPVMAAVE 210 Query: 193 WFEIIIRGRGSHGAMPEAAVDPLNVAAHLHLALQGILSREISPIDNAVLTIGVMEGGTTN 252 FE + G+G HGAMP VD L ++A + +ALQGI++R ++P+ +AV+T+G ++ GT Sbjct: 211 HFECQLFGQGGHGAMPHQTVDTLVISAQIVMALQGIVARNLNPLQSAVVTVGQLQSGTAF 270 Query: 253 NVIPDTARLKGSVRTFNAALRDKVETRIREISAGIGDTFQAKVEVIYTRGCPEVKTDGGL 312 NVIPD+A +G+VR F+ + RI EI GI + A + Y P V D L Sbjct: 271 NVIPDSAYFRGTVRYFDPSFAGYFAQRIEEIIKGICQSHGANYQFTYENIYPPVVNDRRL 330 Query: 313 NEQMRASIGNTFGDGSYIGITELVPGGKLMGSEDFAFVSQAVPSTTVFLNAGNAEEGYSY 372 + +R++ + + L P + + ED +F QAVP FL + N + G +Y Sbjct: 331 ADLVRSAAADVL-----LTDDHLQPDYQTLAGEDMSFFLQAVPGCYFFLGSANGDLGLAY 385 Query: 373 PVHHPKTMFSDEVLHRG 389 P HHP+ F + VL G Sbjct: 386 PHHHPRFNFDEAVLPVG 402 >FN1063 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 394 Score = 258 bits (658), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 151/400 (37%), Positives = 224/400 (56%), Gaps = 11/400 (2%) Query: 7 DFLSMAHQLQDKMVMYRHHLHAVPELDLSLPRTTAYVKEVLESMGLK-PAPVGESGLMVT 65 + L +++ K++ +R LH +PEL+L LP+TT Y++E L+ MG++ V + ++ Sbjct: 2 NVLEEVKKIEQKIIQWRRDLHKIPELNLYLPKTTKYIEEKLKKMGIEYKTLVNGNAIVGL 61 Query: 66 IGGQHAGKVILVRADMDGLPIQEETELSYASLN-GNMHACGHDMHTSMLLGAAEILKASE 124 I G GK I +RADMD LPI+EET L ++S + G MHACGHD HT+MLLGAA+IL + Sbjct: 62 IKGNSEGKTIGLRADMDALPIEEETGLEFSSTHKGCMHACGHDGHTAMLLGAAKILNENR 121 Query: 125 GQLRGTVKLMFQPGEETLHGAKMMLENGILDNPKVDAAMMLHVLTGMPLPIGQFVVPEEG 184 + +G VKL+FQPGEE GA M+E G ++NPK+D + LH + +G+ + + Sbjct: 122 DKFKGNVKLLFQPGEEYPGGALPMIEEGAMENPKIDVVIGLHEGV-IDERVGKGKIAYKD 180 Query: 185 GAISASSDWFEIIIRGRGSHGAMPEAAVDPLNVAAHLHLALQGILSREISPIDNAVLTIG 244 G + AS D F I ++G+G HGA P+ VDP+ +A+ + L+LQ I SREI+ + ++++ Sbjct: 181 GCMMASMDRFLIKVKGKGCHGAYPQMGVDPIVIASEIILSLQKISSREINTNEPIIVSVC 240 Query: 245 VMEGGTTNNVIPDTARLKGSVRTFNAALRDKVETRIREISAGIGDTFQAKVEVIYTRGCP 304 + GG + N+IPD L+G+VR N R + RI EI GI + E+ Y P Sbjct: 241 RINGGFSQNIIPDMVELEGTVRATNNETRKFIANRIEEIVKGITSANRGSYEIEYNFKYP 300 Query: 305 EVKTDGGLNEQMRASIGNTFGDGSYIGITEL-VPGGKLMGSEDFAFVSQAVPSTTVFL-N 362 V D N+ S G+ + I EL P +MG ED A+ + P T FL N Sbjct: 301 AVINDKEFNKFFLESAKKIIGEEN---IFELPTP---VMGGEDMAYFLEKAPGTFFFLSN 354 Query: 363 AGNAEEGYSYPVHHPKTMFSDEVLHRGXXXXXXXXRDWLE 402 +G YP H PK + H G D+L+ Sbjct: 355 PKVYPDGKVYPHHSPKFDVDENYFHIGVALFVQTVLDYLK 394 >CAC1014 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 396 Score = 256 bits (653), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 143/372 (38%), Positives = 218/372 (58%), Gaps = 12/372 (3%) Query: 7 DFLSMAHQLQDKMVMYRHHLHAVPELDLSLPRTTAYVKEVLESMGLKPAPVGESGLMVTI 66 +++ A + D++V R H PEL L RT++ VKE L++ G++ ++G+ I Sbjct: 2 NYIEEAKVMYDELVAIRRDFHEHPELGFELERTSSKVKEFLKNEGIEYYETAKTGICAII 61 Query: 67 GGQHAGKVILVRADMDGLPIQEETE-LSYAS-LNGNMHACGHDMHTSMLLGAAEILKASE 124 G++ GK + +R DMD LP+ E E SY S +NG MHACGHD HT++L+GAA++L + Sbjct: 62 RGKNTGKTVGLRGDMDALPLMENNENRSYCSKVNGRMHACGHDAHTTILMGAAKLLNKMK 121 Query: 125 GQLRGTVKLMFQPGEETLHGAKMMLENGILDNPKVDAAMMLHVLTGMPLPIGQFVVPEEG 184 +L+G VKL F+P EET GA++M+E G+L+NP VDA + LHV + G+ + + Sbjct: 122 DELQGNVKLFFEPAEETTGGAQIMIEEGVLENPHVDAVIGLHV--SEDIECGKIGIKK-- 177 Query: 185 GAISASSDWFEIIIRGRGSHGAMPEAAVDPLNVAAHLHLALQGILSREISPIDNAVLTIG 244 G ++A+S+ F I I+GRG+HGA P A VDP+ A ++ LQ ++SREISP++ AVLTIG Sbjct: 178 GVVNAASNPFTITIKGRGAHGAHPNAGVDPIVAACNIVNMLQTLVSREISPVNPAVLTIG 237 Query: 245 VMEGGTT-NNVIPDTARLKGSVRTFNAALRDKVETRIREISAGIGDTFQAKVEVIYTRGC 303 + GGTT NVIP+ A++ G +RT R+ + R++E+ G + + Sbjct: 238 YIHGGTTAQNVIPEDAKIGGIIRTMKKEDREFAKKRLKEMVEGAATAMRTSASIDIEESY 297 Query: 304 PEVKTDGGLNEQMRASIGNTFGDGSYIGITELVPGGKLMGSEDFAFVSQAVPSTTVFLNA 363 P + D + E ++ N + + I + E P MG E FA+ S PS +L A Sbjct: 298 PCLYNDDNMFEMFKSLAKNLLKEENVIALDE--PS---MGVESFAYFSMERPSVFYYLGA 352 Query: 364 GNAEEGYSYPVH 375 N E+G P H Sbjct: 353 RNEEKGIVNPAH 364 >PH1043 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 387 Score = 254 bits (649), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 146/384 (38%), Positives = 221/384 (57%), Gaps = 19/384 (4%) Query: 9 LSMAHQLQDKMVMYRHHLHAVPELDLSLPRTTAYVKEVLESMGLKPAPVGESGLMVTIGG 68 + A +LQ +V R H PEL RT+ V+E L+ +G + ++G++ + G Sbjct: 6 IKRAKELQGYIVEKRRDFHMYPELKYEEERTSKIVEEELKKLGYEVVRTAKTGVIGILKG 65 Query: 69 QHAGKVILVRADMDGLPIQEETELSYAS-LNGNMHACGHDMHTSMLLGAAEILKASEGQL 127 + GK + +RADMD LPIQEE ++ Y S + G MHACGHD HT+MLLGAA+IL + +L Sbjct: 66 KEDGKTVALRADMDALPIQEENDVPYKSRVPGKMHACGHDAHTAMLLGAAKILAEMKDEL 125 Query: 128 RGTVKLMFQPGEETLHGAKMMLENGILDNPKVDAAMMLHVLTGMPLPIGQFVVPEEGGAI 187 +GTVKL+FQP EE GAK ++E G LD+ VDA +HV +P ++ + G + Sbjct: 126 QGTVKLIFQPAEEGGLGAKKIVEEGHLDD--VDAIFGIHVWAELP----SGIIGIKSGPL 179 Query: 188 SASSDWFEIIIRGRGSHGAMPEAAVDPLNVAAHLHLALQGILSREISPIDNAVLTIGVME 247 AS+D F ++I+G+G HGA P ++DP+ +A L A Q I+SRE+ P+ AVL++ ++ Sbjct: 180 LASADAFRVLIKGKGGHGAAPHLSIDPIALAVDLVNAYQKIISREVDPLQPAVLSVTSIK 239 Query: 248 GGTTNNVIPDTARLKGSVRTFNAALRDKVETRIREISAGI--GDTFQAKVEVIYTRGCPE 305 GTT NVIP++A + G++RTF+ +RD + R++EI+ G + K E+ P Sbjct: 240 AGTTFNVIPESAEILGTIRTFDEEVRDYIVRRMKEITENFANGMRCEGKFELTIEHIPPT 299 Query: 306 VKTDGGLNEQMRASIGNTFGDGSYIGITELVPGGKLMGSEDFAFVSQAVPSTTVFLNAGN 365 + NE++ F + E+ MG+EDFAF + P +FL N Sbjct: 300 IN-----NEKL-----ANFARDVLKVLGEIREPKPTMGAEDFAFYTTKAPGLFIFLGIRN 349 Query: 366 AEEGYSYPVHHPKTMFSDEVLHRG 389 E+G YP HHPK +++L G Sbjct: 350 EEKGIIYPHHHPKFNVDEDILWMG 373 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,816,515 Number of extensions: 1349150 Number of successful extensions: 2689 Number of sequences better than 1.0e-05: 71 Number of HSP's gapped: 2438 Number of HSP's successfully gapped: 71 Length of query: 405 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 296 Effective length of database: 25,807,025 Effective search space: 7638879400 Effective search space used: 7638879400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)