BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0502.0005.p (502 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 227 4e-59 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 225 1e-58 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 213 6e-55 Rv0783c [GEPR] COG0477 Permeases of the major facilitator superf... 212 1e-54 MT0807 [GEPR] COG0477 Permeases of the major facilitator superfa... 212 1e-54 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 227 bits (578), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 125/370 (33%), Positives = 198/370 (53%) Query: 34 VGMIMVILDGTVVNVAVPKLVEYFNTDLKTVQWAITGYTLALAAVIPLAGYMTDRFGSKQ 93 +G + IL+ T++NVA+P +++ F QW TG+ L +IP+ ++ +RF ++Q Sbjct: 19 MGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLVNGVMIPMTAFLIERFTTRQ 78 Query: 94 VFVATVAMFVLGSMLCSIAQTSSQLVLFRVIQGLGGGMVAPIGMAMVFRLAPPERRGSIM 153 +++ + F +G+ + A + L+ R++Q +G G+V P+ +V L P ERRG M Sbjct: 79 LYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAM 138 Query: 154 GLLGIPMLLAPALGPVLSGWLVEYVSWHWIFLINLPIGIVGITLGIKYLPKTETKGRAHL 213 GL+G+ M APA+GP LSGW+V+ W +F I +P I+ I + I L + L Sbjct: 139 GLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILDIVVAIFLLKNVGKRTFPKL 198 Query: 214 DILGIILAPVAFSMLAYGVNQGGGESWSSTXXXXXXXXXXXXXXXFIIVELLHKHPLLEL 273 DILG+I++ V F L G + G W + FI + +K PLL Sbjct: 199 DILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLVVLGIFIRYQSSNKAPLLNF 258 Query: 274 RVFRSSDFTRGIILSWVTQAALFGSMLFVPLYLQQIRNYTPLETGLILLPQALASGIGMP 333 +VF+ F +S+ LFG ML +P++LQ +R ++PLE+GL+LLP AL + I P Sbjct: 259 KVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSPLESGLVLLPGALVTAILSP 318 Query: 334 LGGRLFDKIGARPLVFVGLSIISTALFMLSNVTVDTGLPFIXXXXXXXXXXXXXXXXPVN 393 + G +FD+ GA+ L VGL I++ + FM +N+ T L FI P+ Sbjct: 319 VTGVMFDRFGAKYLSLVGLIIMAGSTFMFTNLDESTTLTFIIIVQTIRSAGMAMVMMPLQ 378 Query: 394 THVLNSAPRE 403 T LNS P + Sbjct: 379 TAALNSLPLK 388 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 225 bits (573), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 137/456 (30%), Positives = 229/456 (50%), Gaps = 27/456 (5%) Query: 44 TVVNVAVPKLVEYFNTDLKTVQWAITGYTLALAAVIPLAGYMTDRFGSKQVFVATVAMFV 103 T +N+A+ L++ N TVQW TGY L L ++P++G + F ++Q+F ++ + Sbjct: 34 TALNIALTDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQLFTVSLIFSI 93 Query: 104 LGSMLCSIAQTSSQLVLFRVIQGLGGGMVAPIGMAMVFRLAPPERRGSIMGLLGIPMLLA 163 LG+ + ++A + S L+ R++Q LG G++ P+ + + PP +RG+ MG +G+ ++ A Sbjct: 94 LGTFIAALAPSFSFLLAARIVQALGTGLLLPLMFNTILVIFPPHKRGAAMGTIGLVIMFA 153 Query: 164 PALGPVLSGWLVEYVSWHWIFLINLPIGIVGITLGIKYLPKTETKGRAHLDILGIILAPV 223 PA+GP SG ++E+++WHWIF I+LP ++ + GI Y+ + +D+L IIL+ + Sbjct: 154 PAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTI 213 Query: 224 AFSMLAYGVNQGGGES--WSSTXXXXXXXXXXXXXXXFIIVELLHKHPLLELRVFRSSDF 281 F + +G + G S WSS F I +L K P++ LR F+ F Sbjct: 214 GFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMF 273 Query: 282 TRGIILSWVTQAALFGSMLFVPLYLQQIRNYTPLETGLILLPQALASGIGMPLGGRLFDK 341 G+I+ ++ + SML +P+YLQ T +GL+LLP + +G P+ GRLFDK Sbjct: 274 ILGVIMVFICMMVILSSMLLLPMYLQGGLVLTAFASGLVLLPGGILNGFMSPVTGRLFDK 333 Query: 342 IGARPLVFVGLSIISTALFMLSNVTVDTGLPFIXXXXXXXXXXXXXXXXPVNTHVLNSAP 401 G + LV G I++ L+ SNVT + I P T+ LN P Sbjct: 334 YGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLP 393 Query: 402 REWVNRVTPLTXXXXXXXXXXXXXGMTGYLTSQIAVHMGKMAAG--------ANPLS--- 450 RE+ T + M G + + +AV + MAAG NP Sbjct: 394 REFYPDGTAIMNTLQQ---------MAGAIGTAVAVSI--MAAGQHDYMSTVKNPADPAV 442 Query: 451 ---AMVLGFNDVFFFSACIAVAGVVISLILRKPKVS 483 A+ G F F+ +A+ G++ + +++ KV Sbjct: 443 IPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVD 478 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 213 bits (542), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 119/375 (31%), Positives = 202/375 (53%), Gaps = 3/375 (0%) Query: 35 GMIMVILDGTVVNVAVPKLVEYFNTDLKTVQWAITGYTLALAAVIPLAGYMTDRFGSKQV 94 G +IL+ +++N A+P+L+ F D W IT + +A+ +PL GY+ D+ G K+ Sbjct: 17 GSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTGYLGDKIGKKEA 76 Query: 95 FVATVAMFVLGSMLCSIAQTSSQLVLFRVIQGLGGGMVAPIGMAMVFRLAPPERRGSIMG 154 ++ + +FVLGS+L +++ L++FR +QG+GGG++ P+ M ++F P RG G Sbjct: 77 YILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLIFDAFPRNERGLATG 136 Query: 155 LLGIPMLLAPALGPVLSGWLVEYVSWHWIFLINLPIGIVGITLGIKYLPKTETKGRAHLD 214 + G+ ++AP +GP L G++VE +W ++FL+N+P G++GI + YLPK G+ LD Sbjct: 137 VWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIYLPKIARSGQIKLD 196 Query: 215 ILGIIL--APVAFSMLAYGVNQGGGESWSSTXXXXXXXXXXXXXXXFIIVELLHKHPLLE 272 G + A V +LA+G + F+ VE + PLL+ Sbjct: 197 RWGFLFVTAGVGSILLAFG-RMSDLTHLTEWINGVLLLLGALCLYVFVQVEKRAEQPLLD 255 Query: 273 LRVFRSSDFTRGIILSWVTQAALFGSMLFVPLYLQQIRNYTPLETGLILLPQALASGIGM 332 L +FR ++ I ++ ++ LF + VPL LQQ+ +Y P+ TGL+ LP AL +G+ M Sbjct: 256 LSLFRIPAYSLSIWVAGISSIGLFAGIFLVPLLLQQVYDYGPIMTGLVFLPSALFTGLTM 315 Query: 333 PLGGRLFDKIGARPLVFVGLSIISTALFMLSNVTVDTGLPFIXXXXXXXXXXXXXXXXPV 392 +GGR+ DK G ++ G+ I + F L + ++TGL +I P Sbjct: 316 SIGGRMLDKRGPSGIMTAGMMIAAVGTFALGYLHLETGLWYIFAWMAIRGVGMGLTTMPA 375 Query: 393 NTHVLNSAPREWVNR 407 T +N+ P ++R Sbjct: 376 TTTGMNAIPEGLISR 390 >Rv0783c [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 540 Score = 212 bits (539), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 114/325 (35%), Positives = 185/325 (56%), Gaps = 2/325 (0%) Query: 37 IMVILDGTVVNVAVPKLVEYFNTDLKTVQWAITGYTLALAAVIPLAGYMTDRFGSKQVFV 96 +M +D TVV+VA V F + V W +TGY LALA VIP AG+ DRFG++++F+ Sbjct: 53 VMAHVDVTVVSVAQRTFVADFGSTQAVVAWTMTGYMLALATVIPTAGWAADRFGTRRLFM 112 Query: 97 ATVAMFVLGSMLCSIAQTSSQLVLFRVIQGLGGGMVAPIGMAMVFRLAPPERRGSIMGLL 156 +V F LGS+LC++A L++FRV+QG GGGM+ P+ A++ R A P+R G +M ++ Sbjct: 113 GSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREAGPKRLGRVMAVV 172 Query: 157 GIPMLLAPALGPVLSGWLVEYVSWHWIFLINLPIGIVGITLGIKYLPKTETKGRAHLDIL 216 GIPMLL P GP+L GWL+ W WIFL+NLP+G+ + L P+ + D + Sbjct: 173 GIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYM 232 Query: 217 GIILAPVAFSMLAYGVNQGGGESWSSTXXXXXXXXXXXXXXXFIIVELLHK--HPLLELR 274 G++L + +GV+ + + ++ HPL+++R Sbjct: 233 GLLLLSPGLATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMR 292 Query: 275 VFRSSDFTRGIILSWVTQAALFGSMLFVPLYLQQIRNYTPLETGLILLPQALASGIGMPL 334 +F++ + + V LFGS L +P YLQQ+ + +P+++G+ ++PQ L + + MP+ Sbjct: 293 LFQNRAVAQANMTMTVLSLGLFGSFLLLPSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPI 352 Query: 335 GGRLFDKIGARPLVFVGLSIISTAL 359 G + D+ G +V VG+ +I+ L Sbjct: 353 AGAMMDRRGPAKIVLVGIMLIAAGL 377 >MT0807 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 545 Score = 212 bits (539), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 114/325 (35%), Positives = 185/325 (56%), Gaps = 2/325 (0%) Query: 37 IMVILDGTVVNVAVPKLVEYFNTDLKTVQWAITGYTLALAAVIPLAGYMTDRFGSKQVFV 96 +M +D TVV+VA V F + V W +TGY LALA VIP AG+ DRFG++++F+ Sbjct: 58 VMAHVDVTVVSVAQRTFVADFGSTQAVVAWTMTGYMLALATVIPTAGWAADRFGTRRLFM 117 Query: 97 ATVAMFVLGSMLCSIAQTSSQLVLFRVIQGLGGGMVAPIGMAMVFRLAPPERRGSIMGLL 156 +V F LGS+LC++A L++FRV+QG GGGM+ P+ A++ R A P+R G +M ++ Sbjct: 118 GSVLAFTLGSLLCAVAPNILLLIIFRVVQGFGGGMLTPVSFAILAREAGPKRLGRVMAVV 177 Query: 157 GIPMLLAPALGPVLSGWLVEYVSWHWIFLINLPIGIVGITLGIKYLPKTETKGRAHLDIL 216 GIPMLL P GP+L GWL+ W WIFL+NLP+G+ + L P+ + D + Sbjct: 178 GIPMLLGPVGGPILGGWLIGAYGWRWIFLVNLPVGLSALVLAAIVFPRDRPAASENFDYM 237 Query: 217 GIILAPVAFSMLAYGVNQGGGESWSSTXXXXXXXXXXXXXXXFIIVELLHK--HPLLELR 274 G++L + +GV+ + + ++ HPL+++R Sbjct: 238 GLLLLSPGLATFLFGVSSSPARGTMADRHVLIPAITGLALIAAFVAHSWYRTEHPLIDMR 297 Query: 275 VFRSSDFTRGIILSWVTQAALFGSMLFVPLYLQQIRNYTPLETGLILLPQALASGIGMPL 334 +F++ + + V LFGS L +P YLQQ+ + +P+++G+ ++PQ L + + MP+ Sbjct: 298 LFQNRAVAQANMTMTVLSLGLFGSFLLLPSYLQQVLHQSPMQSGVHIIPQGLGAMLAMPI 357 Query: 335 GGRLFDKIGARPLVFVGLSIISTAL 359 G + D+ G +V VG+ +I+ L Sbjct: 358 AGAMMDRRGPAKIVLVGIMLIAAGL 382 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,112,401 Number of extensions: 1320061 Number of successful extensions: 4543 Number of sequences better than 1.0e-05: 133 Number of HSP's gapped: 4454 Number of HSP's successfully gapped: 134 Length of query: 502 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 391 Effective length of database: 25,576,357 Effective search space: 10000355587 Effective search space used: 10000355587 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)