BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0017.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yfjR [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 167 2e-41 PA2199 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 164 1e-40 SMa0237 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 84 3e-16 PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 82 1e-15 AGl3303 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 81 2e-15 >BS_yfjR [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 233 Score = 167 bits (423), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 0/245 (0%), Positives = 0/245 (0%), Gaps = 5/245 (2%) Query: 28 LTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVVGSED-FLERLG 86 +T R D+ ++ + V Sbjct: 1 MTQAERRHDRLAVRLSLIISRLVAGETLSVRKLAAEFGVSVRTLRRDFRERLMYLDLEYQ 60 Query: 87 PGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQEAKER 146 G + + + R G + + R A P Sbjct: 61 SGYCRLRTAGSEMQMVPDVLIFAHRSGLAGLFPGLDRRLVNALLMCDESPCVIAPANPVP 120 Query: 147 VRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPKAVVN 206 + R I ++ + + + + AV Sbjct: 121 SPSGALSF-WRLIQAITGRRRVTLIAEGRRCERLAPCRLLIHQQTWYLVAEHEGHIAVFT 179 Query: 207 MLTSTLFPAPIYQSYGKMIAEKTANISESKIP--QKDVGLFLAAAQNAQFPAPLAGQLLD 264 L P+ +++ + + I Sbjct: 180 -LDEIHLIQPLQETFRRNDSLCRLVEDPVFIQAL 238 Query: 265 MLESG 269 Sbjct: 239 243 >PA2199 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 291 Score = 164 bits (416), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 99/246 (40%), Positives = 149/246 (60%), Gaps = 4/246 (1%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVVGSEDFL 82 QA +TV+NR+A +PLVA GA +A + +V++++ DD+A+ V+ L Sbjct: 21 QAGLEVTVWNRSAAACEPLVALGAARAEEVGELFGLD-VVISMLADDQAIRGVLLDSGAL 79 Query: 83 ERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQE 142 ER PG IH+SMST+S D L H R G +V APVFGR + A A +L I V GP+E Sbjct: 80 ERARPGLIHLSMSTLSLDCVEALDQAHQRQGLAFVAAPVFGRTDVAEAGKLNIVVGGPEE 139 Query: 143 AKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPK 202 A E+V+ +LE MG + F FG++ A+ VK+ GNF+I SA S+ E++ + +R G++P Sbjct: 140 AIEQVKALLEIMGQKTWF-FGKDPRGAMAVKISGNFMIASAIESMGESVALVKRLGVEPG 198 Query: 203 AVVNMLTSTLFPAPIYQSYGKMIAEKTANISESKIP--QKDVGLFLAAAQNAQFPAPLAG 260 + +++STLF AP+Y++YG I E+ + ++ KDV L L+A + P PLA Sbjct: 199 RFMELMSSTLFDAPVYRNYGPQIVEQRFTPARFRLVLGLKDVDLALSAGKRHNVPLPLAS 258 Query: 261 QLLDML 266 L D+L Sbjct: 259 LLHDVL 264 >SMa0237 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 293 Score = 83.6 bits (205), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 76/251 (30%), Positives = 118/251 (47%), Gaps = 8/251 (3%) Query: 24 ARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVVGSEDFLE 83 A +++TV+NR A KA+PL A GA AA PADAV IV T++ + +A+ V+ + Sbjct: 26 AGFSVTVWNRDAAKAEPLAADGADIAASPADAVAGAAIVFTMLTNGQAVSEVLFERGVAD 85 Query: 84 RLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQEA 143 L G I V S+++P R+ A A G +++APV G AAA L I G A Sbjct: 86 SLAEGRIVVDCSSIAPQIAREHARRLAEKGIRHLDAPVSGGVVGAAAGTLAIMAGGDGAA 145 Query: 144 KERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDPKA 203 E ++ V +G + G +GA + KL ++ ++ EA+ + E GG A Sbjct: 146 VESLKEVFAVLGR--VTHVG-PSGAGQVCKLANQQIVAVTIGAVAEAMVLVEAGGASRAA 202 Query: 204 VVNMLTSTLFPAPIYQSYG-KMIAEKTANISESKIPQKDVGLFLAAAQNAQFPAPLAGQL 262 + + + I + +G +M+ A S KD+ +A A PL Q+ Sbjct: 203 FRDAIRGGFAESRILELHGARMVERNFAPGGASNNQLKDLNAVMAMADELSLELPLTRQV 262 Query: 263 ----LDMLESG 269 D +ESG Sbjct: 263 RQEFADFVESG 273 >PA3569 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 298 Score = 81.6 bits (200), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 74/247 (29%), Positives = 121/247 (48%), Gaps = 15/247 (6%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESV-VGSEDF 81 +A + + V++ LV QGA A +V++++ + +ES+ +G + Sbjct: 22 KAGHRVNVFDLQPKAVLGLVEQGAQGADSALQCCEGAEVVISMLPAGQHVESLYLGDDGL 81 Query: 82 LERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQ 141 L R+ + + ST++P+T RK+A+ A G ++APV G A A L V GP Sbjct: 82 LARVAGKPLLIDCSTIAPETARKVAEAAAAKGLTLLDAPVSGGVGGARAGTLSFIVGGPA 141 Query: 142 EAKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFL--IVSAGRSLEEALGMAERGGL 199 E R RPVLE MG R IF G+ GA + K+ N L I+ AG + EAL + + GL Sbjct: 142 EGFARARPVLENMG-RNIFHAGDH-GAGQVAKICNNMLLGILMAGTA--EALALGVKNGL 197 Query: 200 DPKAVVNMLTSTL---FPAPIYQSYGKMIAEKTANIS-----ESKIPQKDVGLFLAAAQN 251 DP + ++ + + +Y + ++ + A+ + ++ KD+GL LA AQ Sbjct: 198 DPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAGGFQVRLMNKDLGLALANAQA 257 Query: 252 AQFPAPL 258 Q PL Sbjct: 258 VQASTPL 264 >AGl3303 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Length = 299 Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 17/246 (6%), Positives = 45/246 (18%), Gaps = 6/246 (2%) Query: 23 QARYALTVYNRTADKAKPLVAQGALQAARPADAVTTGGIVVTLVWDDEALESVV-GSEDF 81 Q+ + T+ L+A+ A + I+ + D + + + Sbjct: 32 QSTLSYTISQLERKIGVALLARTTRSVAPTGSGLRLLEILGPALRDLREELLRLQEARET 91 Query: 82 LERLGPGGIHVSMSTVSPDTGRKLADLHARHGSVYVEAPVFGRPEAAAAKQLWIPVAGPQ 141 + + + + I Sbjct: 92 IAGVVRLTMIPVAFESIVRPMLPSFCQRYPDVVFEISTSERLDDIVTEGFDGGIRFGTLI 151 Query: 142 EAKERVRPVLEAMGARGIFDFGEEAGAAVLVKLIGNFLIVSAGRSLEEALGMAERGGLDP 201 + P+ + + + + + L L + Sbjct: 152 DKDMVALPLTASTPI--VIAGS-ASYFENYPIPLAPEHLAGHRCILYRYLSSSRLFRWPL 208 Query: 202 KAVVNMLTSTLFPAPIYQSYGKMIAEKTANI-SESKIPQKDVGLFLAAAQNAQFPAPLAG 260 PA I + + + + + A L Sbjct: 209 AKGSQKFEYKGDPALILDDGAAIRSAVMDGLGIGFLLRSQVASDLEAGNLREVLTDWL-T 267 Query: 261 QLLDML 266 L Sbjct: 268 DLPGFS 273 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,537,104 Number of extensions: 761979 Number of successful extensions: 1776 Number of sequences better than 1.0e-05: 57 Number of HSP's gapped: 1695 Number of HSP's successfully gapped: 57 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)