BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0015.p (216 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1678 [S] COG1284 Uncharacterized conserved protein 97 2e-20 L21717 [S] COG1284 Uncharacterized conserved protein 86 3e-17 L20683 [S] COG1284 Uncharacterized conserved protein 79 5e-15 lin2023 [S] COG1284 Uncharacterized conserved protein 78 1e-14 SPy2153 [S] COG1284 Uncharacterized conserved protein 62 6e-10 >BH1678 [S] COG1284 Uncharacterized conserved protein Length = 290 Score = 97.4 bits (241), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 59/183 (32%), Positives = 100/183 (54%) Query: 6 RLTIILSGTLLLAFTYYHINYQNHLTEGGFVGLSLLGKYVLGISPSLTILILDIPVLLIA 65 + IL G+ + +F + N +N+L EGGF G++L+ ++ I+P++T L+L+IP+LLI Sbjct: 9 NIVFILLGSAIFSFGLVYFNMENNLAEGGFTGITLILYFMFQINPAVTNLVLNIPILLIG 68 Query: 66 MIFKGRAFVLNTFISVGAFTVFYSLMERYSGWVINLQDNLPLAALLSGVLTXXXXXXXXX 125 GR ++ T I + +VF + +R+ I L D++ LAAL +GV Sbjct: 69 WKILGRVTLIYTIIGTVSVSVFLEMFQRWKFMDIPLHDDMTLAALFAGVFVGTGLGIVFR 128 Query: 126 XXXXXXXDDILSLLISEWKGIKVGTVFILMDVIVLALSLFYMPLKETLYTVMAVVVAGYV 185 DI++ L + G +G + D +V+A SL Y+ +E +YT++AV +A V Sbjct: 129 FGGTTGGVDIIAKLGFRYLGWSMGKTMFMFDAVVIASSLIYLNYREAMYTLLAVFIAAKV 188 Query: 186 ITF 188 I F Sbjct: 189 IDF 191 >L21717 [S] COG1284 Uncharacterized conserved protein Length = 311 Score = 86.3 bits (212), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 16/181 (8%), Positives = 32/181 (17%), Gaps = 1/181 (0%) Query: 13 GTLLLAFTYYHINYQNHLTEGGFVGLSLLGKYVLGISPSLTILILDIPVLLIAMIFKGRA 72 L + + L L+G ++ I + I + I K Sbjct: 9 LLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKLDV 68 Query: 73 FVLNTFISVGAFTVFYSLMERYSGWVINLQDNLPLAALLSGVLTXXXXXXXXXXXXXXXX 132 T I + + + +P + Sbjct: 69 HDQETKIQDPKDNKKTINNPDHL-KIYDSIKKVPNVKTVEFSSKEAQLKELTKALGNNWN 127 Query: 133 DDILSLLISEWKGIKVGTVFILMDVIVLALSLFYMPLKETLYTVMAVVVAGYVITFTTSL 192 I + + +S K T + T Sbjct: 128 LFKGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQGIRTWG 187 Query: 193 G 193 Sbjct: 188 T 188 >L20683 [S] COG1284 Uncharacterized conserved protein Length = 311 Score = 79.0 bits (193), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 14/189 (7%), Positives = 38/189 (20%), Gaps = 1/189 (0%) Query: 5 LRLTIILSGTLLLAFTYYHINYQNHLTEGGFVGLSLLGKYVLGISPSLTILILDIPVLLI 64 L L + + G F+ + L + + ++ + + + Sbjct: 10 LESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAYMKLDVH 69 Query: 65 AMIFKGRAFVLNTFISVGAFTVFYSLMERYSGWVINLQDNLPLAALLSGVLTXXXXXXXX 124 K + N + + + + A L + Sbjct: 70 DQETKIQDPKDNKKTINNPDHLKIYDSIKKV-PNVKTVEFSSKEAQLKELTKALGNNWNL 128 Query: 125 XXXXXXXXDDILSLLISEWKGIKVGTVFILMDVIVLALSLFYMPLKETLYTVMAVVVAGY 184 D + + +K T I + + + + G Sbjct: 129 FKGDANPLYDAYIVEADKPANVKSVTSKISKLSGIEKATYGGANTAKLFQIAQGIRTWGT 188 Query: 185 VITFTTSLG 193 Sbjct: 189 AAAILLIFV 197 >lin2023 [S] COG1284 Uncharacterized conserved protein Length = 288 Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 53/186 (28%), Positives = 94/186 (50%), Gaps = 1/186 (0%) Query: 6 RLTIILSGTLLLAFTYYHINYQNHLTEGGFVGLSLLGKYVLGISPSLTILILDIPVLLIA 65 + I+ GT + AF + N N+L EGG G++L + I P+ + L+L+IP+ +I Sbjct: 9 NICFIMLGTAIYAFGLVNFNIANNLGEGGLAGVTLFLLHFFQIDPAYSNLVLNIPLFIIG 68 Query: 66 MIFKGRAFVLNTFISVGAFTVFYSLMERYSGWVINLQDNLPLAALLSGVLTXXXXXXXXX 125 G ++ T I + ++F + +R + ++L +L L AL +G + Sbjct: 69 WRVLGSRSLIYTGIGTVSLSLFLWIFQRIP-YTLDLHSDLLLVALFAGGFSGIGLGLVFR 127 Query: 126 XXXXXXXDDILSLLISEWKGIKVGTVFILMDVIVLALSLFYMPLKETLYTVMAVVVAGYV 185 DI++ L++ KGI +G +D IVLA SL Y+ +++ +YT++AV + V Sbjct: 128 YGGTTGGSDIIAKLLNHTKGISMGRTLFAIDAIVLAASLSYLDVRQVMYTLVAVFIGSRV 187 Query: 186 ITFTTS 191 I F Sbjct: 188 IDFVQE 193 >SPy2153 [S] COG1284 Uncharacterized conserved protein Length = 290 Score = 62.4 bits (150), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 48/182 (26%), Positives = 92/182 (50%), Gaps = 3/182 (1%) Query: 13 GTLLLAFTYYHINYQNHLTEGGFVGLSLLGKYVLGISPSLTILILDIPVLLI-AMIFKGR 71 G + F + + N N L EGG G++L+ GI+P+ + L+ ++P+ ++ A IF R Sbjct: 19 GVAIYTFGFVNFNMANALAEGGVAGITLILHAHFGINPAYSSLLFNLPLFILGAKIFGKR 78 Query: 72 AFVLNTFISVGAFTVFYSLMERYSGWVINLQDNLPLAALLSGVLTXXXXXXXXXXXXXXX 131 + L + +V + F + ++ + L++++ L A+++G+ + Sbjct: 79 SLALTIYGTV-LMSAFIWMWQKVP-IELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTG 136 Query: 132 XDDILSLLISEWKGIKVGTVFILMDVIVLALSLFYMPLKETLYTVMAVVVAGYVITFTTS 191 DI+ + E G K+G +L+D +VL SL Y+ LK LYT++A V +I+ + Sbjct: 137 GTDIIGRIAEEKFGAKLGQTLLLVDALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQN 196 Query: 192 LG 193 G Sbjct: 197 GG 198 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.144 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 14,436,904 Number of extensions: 556152 Number of successful extensions: 2149 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 2134 Number of HSP's successfully gapped: 26 Length of query: 216 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 114 Effective length of database: 26,614,363 Effective search space: 3034037382 Effective search space used: 3034037382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 113 (48.1 bits)