BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0006.p (646 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_fruA_3 [G] COG1299 Phosphotransferase system, fructose-specif... 235 2e-61 BB0629_3 [G] COG1299 Phosphotransferase system, fructose-specifi... 214 3e-55 SA0655_3 [G] COG1299 Phosphotransferase system, fructose-specifi... 209 1e-53 SPy0855_3 [G] COG1299 Phosphotransferase system, fructose-specif... 206 1e-52 lin2429_3 [G] COG1299 Phosphotransferase system, fructose-specif... 197 3e-50 >BS_fruA_3 [G] COG1299 Phosphotransferase system, fructose-specific IIC component Length = 339 Score = 235 bits (599), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 149/343 (43%), Positives = 208/343 (60%), Gaps = 13/343 (3%) Query: 309 IGSRIYKDLMNGISHMLPFVVGGGILLAISFLIEQVASVD------NPIVKLLQTIGGGD 362 IG+ YK LM+G+S+MLPFVVGGGIL+AISF + S D N L IGG D Sbjct: 1 IGNTFYKHLMSGVSNMLPFVVGGGILVAISFF-WGIHSADPNDPSYNTFAAALNFIGG-D 58 Query: 363 GAFHFLIPILAGFIALSIGDRPALMPGMVGGYMXXXXXXXXXXXXXXXXXXXYVVILLRK 422 A ++ +LAGFIA+SI DRP PGMVGG+M YVVILL+K Sbjct: 59 NALKLIVAVLAGFIAMSIADRPGFAPGMVGGFMATQANAGFLGGLIAGFLAGYVVILLKK 118 Query: 423 TFAGLPKTLDGLKPILLYPVFSLLITGGIM-FYLFDPVFSWINEGLIDGLNHLGTGNAVX 481 F +P++LDGLKP+L+YP+F + ITG +M F + PV +++N L + L LGTGN V Sbjct: 119 VFTFIPQSLDGLKPVLIYPLFGIFITGVLMQFVVNTPVAAFMNF-LTNWLESLGTGNLVL 177 Query: 482 XXXXXXXXXXXXXXXPFNKAAYTFAIGVFTSSGNTNGLXXXXXXXXXXXPPLAIALAXXX 541 P NKAA+TF I + + N PPL IALA Sbjct: 178 MGIILGGMMAIDMGGPLNKAAFTFGIAMIDAG---NYAPHAAIMAGGMVPPLGIALATTI 234 Query: 542 XXXXXXXXXRKSGLTNYVLGLSFITEGAIPFAAADPLRVLTSCIIGSAVAGGLTQLWNLN 601 R++G+T Y +G +F+TEGAIPFAAADPLRV+ + ++G+AVAGGLT+ + + Sbjct: 235 FRNKFTQRDREAGITCYFMGAAFVTEGAIPFAAADPLRVIPAAVVGAAVAGGLTEFFRVT 294 Query: 602 VPAPHGGIFVAALSSNALLFILAVLIGSVISGLTLGLWKKPLK 644 +PAPHGG+FVA ++++ +L++L+++IG+V+ + LG+ KKP+ Sbjct: 295 LPAPHGGVFVAFITNHPMLYLLSIVIGAVVMAIILGIVKKPVT 337 >BB0629_3 [G] COG1299 Phosphotransferase system, fructose-specific IIC component Length = 336 Score = 214 bits (546), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 132/338 (39%), Positives = 187/338 (55%), Gaps = 11/338 (3%) Query: 314 YKDLMNGISHMLPFVVGGGILLAISFLIE-QVASVDNP----IVKLLQTIGGGDGAFHFL 368 YK LMNG+S MLPFVV GGI++AISF+ + +++P I +L IGGG AF + Sbjct: 1 YKHLMNGVSFMLPFVVSGGIIIAISFMFGIKAFDINDPSYNKIADILMQIGGGS-AFALM 59 Query: 369 IPILAGFIALSIGDRPALMPGMVGGYMXXXXXXXXXXXXXXXXXXXYVVILLRKTFAGL- 427 IPILAG+I+ SI +RP L PGM+ G M YV + ++K + Sbjct: 60 IPILAGYISFSIAERPGLAPGMITGLMMNNGNAGFLGGILAGFISGYVTLTVKKISDKII 119 Query: 428 PKTLDGLKPILLYPVFSLLITGGIMFYLFDPVFSWINEGLIDGLNHLGTGNAVXXXXXXX 487 P L G+ P+L YP S++I+G +++ + P+ S INE + + LN L N Sbjct: 120 PSNLRGINPVLTYPFLSVIISGILIYGMLSPI-SVINESITNMLNQLSGTNMAILGALLG 178 Query: 488 XXXXXXXXXPFNKAAYTFAIGVFTSSGNTNGLXXXXXXXXXXXPPLAIALAXXXXXXXXX 547 P NKAAY F I + T+ N + PP+ IALA Sbjct: 179 GMMAIDMGGPVNKAAYAFGIAMITAK---NYIPHASIMAGGMIPPIGIALATSLFKNRFS 235 Query: 548 XXXRKSGLTNYVLGLSFITEGAIPFAAADPLRVLTSCIIGSAVAGGLTQLWNLNVPAPHG 607 R+SG Y LG FITEG IPFAAADPLRV+ +CI+GS+V G ++ L+ + V APHG Sbjct: 236 KEERESGKVCYFLGACFITEGVIPFAAADPLRVIPACILGSSVGGFISALFKVEVIAPHG 295 Query: 608 GIFVAALSSNALLFILAVLIGSVISGLTLGLWKKPLKT 645 GIF+ + N L++I ++L+GS+I+ + +G+ KK K Sbjct: 296 GIFILPIVVNPLMWITSILVGSIITAVLIGILKKEYKN 333 >SA0655_3 [G] COG1299 Phosphotransferase system, fructose-specific IIC component Length = 346 Score = 209 bits (533), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 135/337 (40%), Positives = 177/337 (52%), Gaps = 11/337 (3%) Query: 314 YKDLMNGISHMLPFVVGGGILLAISFLI-----EQVASVDNPIVKLLQTIGGGDGAFHFL 368 YK LMNG+S+MLP V+ GGIL+AI FL +S N + L IG AF + Sbjct: 1 YKHLMNGVSNMLPLVISGGILMAIVFLFGANSFNPKSSEYNAFAEQLWNIGS-KSAFALI 59 Query: 369 IPILAGFIALSIGDRPALMPGMVGGYMXXXXXXXXXXXXXXXXXXXYVVILLRKTFAGLP 428 IPIL+GFIA SI D+P G+VGG + Y+ ++ LP Sbjct: 60 IPILSGFIARSIADKPGFASGLVGGMLAISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLP 119 Query: 429 KTLDGLKPILLYPVFSLLITGGIMFYLFDPVFSWINEGLIDGLNHLGTGNAVXXXXXXXX 488 + L+GLKP L+YP+ ++ TG +M Y+F+P SW+N L+DGLN+L N V Sbjct: 120 QALEGLKPTLIYPLLTVTATGLLMIYVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGA 179 Query: 489 XXXXXXXXPFNKAAYTFAIGVFTSSGNTNGLXXXXXXXXXXXPPLAIALAXXXXXXXXXX 548 PFNKAAY FA G N PPLAIA A Sbjct: 180 MMAIDMGGPFNKAAYVFATGALIEG---NAAPITAAMIGGMIPPLAIATAMLIFRRKFTK 236 Query: 549 XXRKSGLTNYVLGLSFITEGAIPFAAADPLRVLTSCIIGSAVAGGLTQLWNLNVPAPHGG 608 R S + NYV+G+SFITEGAIPFAAADPLRV+ S +IGS + G + + APHGG Sbjct: 237 EQRGSIIPNYVMGMSFITEGAIPFAAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGG 296 Query: 609 IFVAALSSNALLF--ILAVLIGSVISGLTLGLWKKPL 643 I V + A L ++A+L+G+++S L GL K L Sbjct: 297 IIVIVGTDGAHLLQTLIALLVGTLVSALIYGLIKPKL 333 >SPy0855_3 [G] COG1299 Phosphotransferase system, fructose-specific IIC component Length = 360 Score = 206 bits (524), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 135/360 (37%), Positives = 190/360 (52%), Gaps = 35/360 (9%) Query: 314 YKDLMNGISHMLPFVVGGGILLAISFLIEQVASVDN----------PIVKLLQTIGGGDG 363 YK LM G+S MLPFV+GGGI++A++FL++ + V N I + IGG Sbjct: 2 YKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSYHEIAAIFMNIGGA-- 59 Query: 364 AFHFLIPILAGFIALSIGDRPALMPGMVGGYMXXXXXXXXXXXXXXXXXXXY-------- 415 AF F++P+LAG+IA SI ++P L+ G V G + Sbjct: 60 AFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFAAGGEVSLGLTGVPSG 119 Query: 416 -------------VVILLRKTFAGLPKTLDGLKPILLYPVFSLLITGGIMFYLFDPVFSW 462 V++ LRK AGLP++L+G+K ILLYP+ +L+TG +M ++ P+ + Sbjct: 120 FLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGVLVTGFLMLFVNIPM-AA 178 Query: 463 INEGLIDGLNHLGTGNAVXXXXXXXXXXXXXXXXPFNKAAYTFAIGVFTSS-GNTNGLXX 521 IN L D L L +AV P NKAAY F G ++ N + Sbjct: 179 INTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYVFGTGTLAATVANGGSVVM 238 Query: 522 XXXXXXXXXPPLAIALAXXXXXXXXXXXXRKSGLTNYVLGLSFITEGAIPFAAADPLRVL 581 PPLA+ +A R+SGLTN V+GLSFITEGAIPF AADP R + Sbjct: 239 AAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMGLSFITEGAIPFGAADPARAI 298 Query: 582 TSCIIGSAVAGGLTQLWNLNVPAPHGGIFVAALSSNALLFILAVLIGSVISGLTLGLWKK 641 S I GSA+ G L L + + APHGGIFV AL+SN +L+++ V+IG+++SG+ G +K Sbjct: 299 PSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPILYLVFVVIGALVSGILFGALRK 358 >lin2429_3 [G] COG1299 Phosphotransferase system, fructose-specific IIC component Length = 340 Score = 197 bits (502), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 142/339 (41%), Positives = 186/339 (54%), Gaps = 14/339 (4%) Query: 314 YKDLMNGISHMLPFVVGGGILLAISFLIEQVASVD----------NPIVKLLQTIGGGDG 363 YK LM+G+SHMLPFV+GGGI +AI+F+++Q+ V N I LL+ IG D Sbjct: 1 YKHLMSGVSHMLPFVIGGGIAIAIAFMLDQILGVPQDQLAKLGSYNEIPALLKQIG--DV 58 Query: 364 AFHFLIPILAGFIALSIGDRPALMPGMVGGYMXXXXXXXXXXXXXXXXXXXYVVILLRKT 423 AF F++P+ AG+IA SI DRP L+ G V G + Y V L++ Sbjct: 59 AFGFMLPVFAGYIAYSISDRPGLVAGFVAGGVASVGGAGFLGALVGGFLAGYAVELIKVM 118 Query: 424 FAGLPKTLDGLKPILLYPVFSLLITGGIMFYLFDPVFSWINEGLIDGLNHLGTGNAVXXX 483 LPKTLDG+K +L YPV S+LI G +M L P+ S +N L D LN L NAV Sbjct: 119 LKKLPKTLDGIKVVLFYPVLSVLIVGLLMLLLNVPM-SALNTWLNDFLNSLSGTNAVILG 177 Query: 484 XXXXXXXXXXXXXPFNKAAYTFAIGVFTSSGNTNGLXXXXXXXXX-XXPPLAIALAXXXX 542 P NKAAY FA G +S T G PPLA +A Sbjct: 178 LLLGAMMAADLGGPINKAAYIFATGTLAASVATGGSAIMAATMAAGMVPPLATFVATLVF 237 Query: 543 XXXXXXXXRKSGLTNYVLGLSFITEGAIPFAAADPLRVLTSCIIGSAVAGGLTQLWNLNV 602 R +GLTN +LG SFITEGAIPFAAADPLR++ S I GSA+ G + N+ V Sbjct: 238 RNKFTAQERDAGLTNSILGASFITEGAIPFAAADPLRMIPSFIAGSAITGAIVMFLNIKV 297 Query: 603 PAPHGGIFVAALSSNALLFILAVLIGSVISGLTLGLWKK 641 APHGG+FV L S +++A++IG++IS +G+ +K Sbjct: 298 LAPHGGVFVIFLVSQPWFYLIAIVIGTIISAALIGVLRK 336 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 41,555,389 Number of extensions: 1694651 Number of successful extensions: 6434 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 6275 Number of HSP's successfully gapped: 144 Length of query: 646 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 533 Effective length of database: 25,345,689 Effective search space: 13509252237 Effective search space used: 13509252237 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)