BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0005.p (303 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2430 [G] COG1105 Fructose-1-phosphate kinase and related fruc... 323 2e-88 SP0876 [G] COG1105 Fructose-1-phosphate kinase and related fruct... 301 9e-82 SPy0854 [G] COG1105 Fructose-1-phosphate kinase and related fruc... 291 7e-79 SA0654 [G] COG1105 Fructose-1-phosphate kinase and related fruct... 232 6e-61 CAC0232 [G] COG1105 Fructose-1-phosphate kinase and related fruc... 133 3e-31 >lin2430 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 307 Score = 323 bits (828), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 172/303 (56%), Positives = 217/303 (71%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYT+TLNPSIDYIV+++ L LG LNRM++D KLPGGKGINVSRVLNQL V + ATGFLG Sbjct: 1 MIYTITLNPSIDYIVQIDQLKLGELNRMKQDYKLPGGKGINVSRVLNQLNVPSLATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG +I WL E I + FV V +DTRINIKLK EETEING GP I Sbjct: 61 GFTGGFIKDWLKNEGIQTGFVIVKDDTRINIKLKHGEETEINGLGPAISEKEIKEFLEVM 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 GDI++ FY ++I CK K+A F+IDTTGQ L DAL P+L+KP Sbjct: 121 DKVTAGDIVILSGSVPPSLGNDFYDKIIQICKEKQANFMIDTTGQELLDALPNRPILIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 NHHELAELFGV + S +++I YG++ +E GA++V+VSMAG+GAL + + VY A+A KG+ Sbjct: 181 NHHELAELFGVKLDSVEDLIPYGKKCLELGAQHVIVSMAGDGALLFTGEDVYFADALKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 +KNSVGAGDSMIAGFVGT T D +++F GVA+G ATAFS DLA++ LI +L Q++I Sbjct: 241 LKNSVGAGDSMIAGFVGTFDKTKDPVKSFAAGVATGGATAFSTDLAEKDLINELLPQVKI 300 Query: 301 EQL 303 ++ Sbjct: 301 TKI 303 >SP0876 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 303 Score = 301 bits (771), Expect = 9e-82, Method: Compositional matrix adjust. Identities = 153/302 (50%), Positives = 197/302 (65%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYTVTLNPSIDYIV ++ + +G +NRM D K GGKGINVSRVL +L + NTATGF+G Sbjct: 1 MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSRVLKRLNIPNTATGFIG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTG++I L EE+I + FV VAEDTRIN+K+KA +ETEINGTGP + Sbjct: 61 GFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEINGTGPTVESVQLEELKAIL 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 D +V Y LI+ + A+ V D GQ L D+L +PLLVKP Sbjct: 121 SSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVCDFEGQTLIDSLDYQPLLVKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 N+HEL +FGV + S DEI Y R L+ GA+ V++SMAG+GAL ++ +G Y A KG Sbjct: 181 NNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISMAGDGALLVTSEGAYFAKPIKGT 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 VKNSVGAGDSM+AGF G + DV+EAF+ GVA G+AT FSDDLA I + ++++ Sbjct: 241 VKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACGTATTFSDDLATAEFIKETYGKVEV 300 Query: 301 EQ 302 E+ Sbjct: 301 EK 302 >SPy0854 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 303 Score = 291 bits (746), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 147/302 (48%), Positives = 197/302 (65%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYTVTLNPSID+IV ++ + LG +NRM D K GGKGINVSR+L +L +++TATGFLG Sbjct: 1 MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSRILQRLDIASTATGFLG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GFTGR+I + L E + +DFV +DTRIN+K+K+ EETE+NG GP+I Sbjct: 61 GFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELNGQGPIISQEQLEDLKTKL 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 D +V Y L+ + A+ V D GQ L DALA PLLVKP Sbjct: 121 SQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVCDFEGQTLIDALAYNPLLVKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 N+HEL +FG + S D++ TY RRL+E GA+ V++SMAG+GAL ++ + Y A KG+ Sbjct: 181 NNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISMAGDGALLVTKEATYFAKPIKGE 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 VKNSVGAGDSM+AGF G + + +EA + GVA G+ATAFSDDLA A I + ++++ Sbjct: 241 VKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACGTATAFSDDLATIAFIKETYHKVEV 300 Query: 301 EQ 302 E+ Sbjct: 301 EK 302 >SA0654 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 306 Score = 232 bits (591), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 124/303 (40%), Positives = 174/303 (57%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 MIYTVT NPSIDY++ D + GLNR K GGKGINVSRVL L V +TA GF G Sbjct: 1 MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSRVLKTLDVESTALGFAG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLKAAEETEINGTGPVIRXXXXXXXXXXX 120 GF G++I L+ I S+F+ V EDTRIN+KLK +ETEIN GP I Sbjct: 61 GFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEINAPGPHITSTQFEQLLQQI 120 Query: 121 XXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPLLVKP 180 DI++ Y+++ A+ V+D + + L PL +KP Sbjct: 121 KNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVVDAEKELAESVLPFHPLFIKP 180 Query: 181 NHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANAPKGQ 240 N EL +F ++ S ++I YGR L++ GA+ V+VS+ G+GA++I + A P+G+ Sbjct: 181 NKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSLGGDGAIYIDKEISIKAVNPQGK 240 Query: 241 VKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRALIGQLKSQIQI 300 V N+VG+GDS +AG V ++ + +AF+ VA G+ATAF +DLA R I ++KSQ+ I Sbjct: 241 VVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACGTATAFDEDLATRDAIEKIKSQVTI 300 Query: 301 EQL 303 L Sbjct: 301 SVL 303 >CAC0232 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 309 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 90/309 (29%), Positives = 152/309 (49%), Gaps = 6/309 (1%) Query: 1 MIYTVTLNPSIDYIVEVEDLTLGGLNRMQRDLKLPGGKGINVSRVLNQLGVSNTATGFLG 60 M+ TVTLNP++D + +++ +G +NR+ GGKGINVS+VL + + TGF+G Sbjct: 1 MVITVTLNPAMDKTLNIDNFNVGVVNRVSNIRYDIGGKGINVSKVLKNFNIESKCTGFIG 60 Query: 61 GFTGRYIGQWLDEEQISSDFVTVAEDTRINIKLK---AAEETEINGTGPVIRXXXXXX-X 116 G + + LD+ I ++F+TV+ +TR N K+ T+IN GP I Sbjct: 61 GMWEKDFREELDKRGIKNEFITVSGNTRTNTKIVDDLNKVYTDINEAGPSISSKELEMFM 120 Query: 117 XXXXXXXXXGDILVXXXXXXXXXXXXFYSRLIAECKSKKAEFVIDTTGQALKDALAQEPL 176 DI+V Y L K + + ++D G+ L + + + P Sbjct: 121 NKFKDMCNKDDIVVLSGGVAPGIPKNIYGTLTKIAKEEGSFVILDAEGELLSEGIKENPY 180 Query: 177 LVKPNHHELAELFGVSIGSRDEIITYGRRLMESGARYVLVSMAGEGALFISDQGVYCANA 236 ++KPN E L + +II + ++ G VL+S+ +GALF++ Y A Sbjct: 181 IIKPNDMEFELLLNKKFKNNTDIIDGAKEVIGLGVSNVLISLGSKGALFVNKDKSYYAKG 240 Query: 237 PKGQVKNSVGAGDSMIAGFVGTLSLTGDVLEAFRTGVASGSATAFSDDLADRAL--IGQL 294 VK++VGAGDSM+A FV + + + E R +A G+A+ ++ +L + +L Sbjct: 241 LVVPVKSTVGAGDSMVAAFVYGIINSLNEKEILRFSIACGAASVSTEGTEACSLEDVKEL 300 Query: 295 KSQIQIEQL 303 + IE++ Sbjct: 301 LKSVVIEEV 309 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,503,578 Number of extensions: 768899 Number of successful extensions: 1759 Number of sequences better than 1.0e-05: 24 Number of HSP's gapped: 1736 Number of HSP's successfully gapped: 30 Length of query: 303 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 197 Effective length of database: 26,153,027 Effective search space: 5152146319 Effective search space used: 5152146319 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)