BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0001.p (366 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2921 [R] COG0628 Predicted permease 219 6e-57 BS_yueF [R] COG0628 Predicted permease 217 2e-56 lin0908 [R] COG0628 Predicted permease 176 4e-44 SA1192 [R] COG0628 Predicted permease 166 5e-41 SP1505 [R] COG0628 Predicted permease 164 2e-40 >BH2921 [R] COG0628 Predicted permease Length = 361 Score = 219 bits (558), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 122/353 (34%), Positives = 202/353 (57%), Gaps = 5/353 (1%) Query: 1 MLQSKYFRTCLAIIAFLTILYLGSKVVFLITPLVAIFNLLLVPMMLSGFMYYLLRPLVNF 60 M Q K+FR I+ L I+YLGS V ++ P+V L P++++G +YYL RP+V Sbjct: 1 MPQGKWFRFGYGIVLVLLIIYLGSLVDWIFRPIVVFVQTLFAPVIIAGILYYLFRPIVGL 60 Query: 61 LESKKLNRMLSILLIYVVFIGLFVLFWVLVWPTLREQIQNFIDNTPYLVQGIQEQFNKLQ 120 L SKK+NR LSIL+++++ IG F F ++V P L+ Q Q+ +N P L+ +++ F LQ Sbjct: 61 L-SKKMNRGLSILILFLMIIGAFTAFSLIVGPELQRQFQSLYNNLPTLINQVRDTFIALQ 119 Query: 121 SDPYFSRFFKGDA----DLTARISGYLSDTITWVTNSMSNLXXXXXXXXXXXATLPIILY 176 S+ F+RF + + ++ +R L+D I + +++ +P IL+ Sbjct: 120 SNELFNRFQESETFQPEEMISRFVNSLNDIIGTIGANITGFIGALANFFIILVLVPFILF 179 Query: 177 YMLKDGHKLSPILLSLVPRKYRKEGQDMLQDIDSALSGFIVTRVLLNVVLGAMLYVGFLI 236 Y+LK+G KL LL L +K + EG+ +L D+D LS +I +++++ +G + Y+ +LI Sbjct: 180 YLLKEGEKLPVQLLKLFNKKQQHEGRLILADMDKMLSSYIQGQIIVSFCVGILCYIAYLI 239 Query: 237 IGLPYSLMLAIISIPLNFIPYVGSLLAAVPVVIVGFIESPTMAIWSXXXXXXXXXXXDNV 296 IGL Y+L+LA++++ N IP+VG + P VIV FI+SP A+ N+ Sbjct: 240 IGLDYALILALVAMFTNVIPFVGPWIGTFPAVIVAFIDSPLTALIVVIVVVVIQQIESNL 299 Query: 297 LSPIIYGKSLDVHPXXXXXXXXXGGDFYGIIGVLIALPVYMILKIIFLRIYEI 349 +SP + GK L +HP G F G++G+L+A+P Y + K+I Y + Sbjct: 300 ISPQVMGKKLAIHPITIIFILLVAGRFAGVVGMLLAVPTYAVGKVIVTHSYRL 352 >BS_yueF [R] COG0628 Predicted permease Length = 369 Score = 217 bits (552), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 119/354 (33%), Positives = 195/354 (55%), Gaps = 5/354 (1%) Query: 1 MLQSKYFRTCLAIIAFLTILYLGSKVVFLITPLVAIFNLLLVPMMLSGFMYYLLRPLVNF 60 ML+SK L I+ L I+++ +KV F+ P + + L PM+++G +Y++ P+V Sbjct: 1 MLKSKVHFWTLQILFVLLIIFVATKVSFVFQPFIVFISTLFFPMLIAGILYFIFNPVVRL 60 Query: 61 LESKKLNRMLSILLIYVVFIGLFVLFWVLVWPTLREQIQNFIDNTPYLVQGIQEQFNKLQ 120 LE KK+ R LSILLIY++FIGL V P + Q+ +N P ++ IQ L Sbjct: 61 LE-KKIPRTLSILLIYLLFIGLLAFISASVGPIITAQVTGLFNNLPDYIKQIQALTKDLS 119 Query: 121 SDPYFSRFFKGD----ADLTARISGYLSDTITWVTNSMSNLXXXXXXXXXXXATLPIILY 176 +F+ D + + ++ +L + +T+S+S + T+P IL+ Sbjct: 120 HSQWFTWMMNQDYVSISKIEQSLTSFLQNLPQNITSSLSAVFGVVTNITLVIITVPFILF 179 Query: 177 YMLKDGHKLSPILLSLVPRKYRKEGQDMLQDIDSALSGFIVTRVLLNVVLGAMLYVGFLI 236 YMLKDGH+ + + ++P YR EG + +D+ L+ + ++L+ + +G ++G+LI Sbjct: 180 YMLKDGHRFPHLAVKILPASYRTEGLKIFKDLSDTLAAYFQGQLLICLFVGTACFIGYLI 239 Query: 237 IGLPYSLMLAIISIPLNFIPYVGSLLAAVPVVIVGFIESPTMAIWSXXXXXXXXXXXDNV 296 GLPY+L+L I+ N IPYVG L A P VIVGF++SP A+++ N+ Sbjct: 240 AGLPYALILGIVMAITNIIPYVGPFLGAAPAVIVGFMDSPAKALFAIIVVVIVQQLDGNL 299 Query: 297 LSPIIYGKSLDVHPXXXXXXXXXGGDFYGIIGVLIALPVYMILKIIFLRIYEII 350 LSP++ GK L+ HP G F GI+G+++A+PVY ++K FL I +I Sbjct: 300 LSPLVIGKRLNTHPLTIILLLIGAGSFGGILGMILAVPVYAVVKAFFLNIVRLI 353 >lin0908 [R] COG0628 Predicted permease Length = 378 Score = 176 bits (447), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 103/355 (29%), Positives = 182/355 (51%), Gaps = 12/355 (3%) Query: 3 QSKYFRTCLAIIAFLTILYLGSKVVFLITPLVAIFNLLLVPMMLSGFMYYLLRPLVNFLE 62 SK F + I+A + +L++ ++ ++ +P+ I + L +P++++GF++YL PLV FLE Sbjct: 8 DSKLFFWTIEILAVVAVLFILLQMKYIFSPIGIIVSTLFMPILVAGFLFYLFNPLVLFLE 67 Query: 63 SKKLNRMLSILLIYVVFIGLFVLFWVLVWPTLREQIQNFIDNTPYLVQGIQEQFNKLQSD 122 +K+ R+LS++LI++ FI L VL + + PTL +Q+ P Q ++ L ++ Sbjct: 68 KRKVPRLLSVILIFIAFIALVVLAVMQLGPTLADQVAELAKAIPGYWQDFEKWLQSLSNN 127 Query: 123 PYFSRFFKGDADLTARISGY-------LSDTITWVTNSMSNLXXXXXXXXXXXATLPIIL 175 D D+ +S +S + V +S + T+P I+ Sbjct: 128 SALK-----DIDMKQELSKLNISLPKIMSVVVDGVASSFGAIISFVSGFVMILVTVPFIV 182 Query: 176 YYMLKDGHKLSPILLSLVPRKYRKEGQDMLQDIDSALSGFIVTRVLLNVVLGAMLYVGFL 235 +YM KDGHK P R E + ++++++ +S +I ++ + + +G ++G+L Sbjct: 183 FYMFKDGHKFVESSGKFFPAGIRTEAKQIIKEMNKTISTYISSQAIDCMFVGLFTFIGYL 242 Query: 236 IIGLPYSLMLAIISIPLNFIPYVGSLLAAVPVVIVGFIESPTMAIWSXXXXXXXXXXXDN 295 IIG PY+L+ I+ N IPY+G L A P VIV SP A+ N Sbjct: 243 IIGQPYALLFGFIAGATNIIPYLGPFLGAAPAVIVALFTSPVQALLVIVVVTIVQQIDSN 302 Query: 296 VLSPIIYGKSLDVHPXXXXXXXXXGGDFYGIIGVLIALPVYMILKIIFLRIYEII 350 +LSP I GKSL +HP G+ GI G+++ +P+Y ++K I + + +I Sbjct: 303 LLSPYIMGKSLSIHPLTIIIILIVAGNLAGIFGMILGVPLYAVVKTIIVNVNRLI 357 >SA1192 [R] COG0628 Predicted permease Length = 402 Score = 166 bits (420), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 87/345 (25%), Positives = 183/345 (53%), Gaps = 4/345 (1%) Query: 9 TCLAIIAFLTILYLGSKVVFLITPLVAIFNLLLVPMMLSGFMYYLLRPLVNFLESKKLNR 68 + +A++ ++++ KV ++ P + +F + P+++S ++YL P+VN +E ++ R Sbjct: 32 SLIALVLLGIVIFIFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPR 91 Query: 69 MLSILLIYVVFIGLFVLFWVLVWPTLREQIQNFIDNTPYLVQGIQEQFNKLQSDPYFSRF 128 + I +IY+ +G+ L L+ P + Q+ + + N+P ++ + +K+ ++ +FS + Sbjct: 92 VAGISIIYLAVVGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSY 151 Query: 129 FKGDAD----LTARISGYLSDTITWVTNSMSNLXXXXXXXXXXXATLPIILYYMLKDGHK 184 + D L +I LS+ + ++ T P +L++MLKDGH Sbjct: 152 YSQINDWLNSLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHH 211 Query: 185 LSPILLSLVPRKYRKEGQDMLQDIDSALSGFIVTRVLLNVVLGAMLYVGFLIIGLPYSLM 244 +++P K+RK+ D+L+ + + +I +++++ +G +L++G+ +IGL YSL+ Sbjct: 212 FKEFSTNIMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLV 271 Query: 245 LAIISIPLNFIPYVGSLLAAVPVVIVGFIESPTMAIWSXXXXXXXXXXXDNVLSPIIYGK 304 LA I+ + +PY+G +A P +++ I SP M + + +SP I GK Sbjct: 272 LASIAAVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGK 331 Query: 305 SLDVHPXXXXXXXXXGGDFYGIIGVLIALPVYMILKIIFLRIYEI 349 +L +HP G GI+GV++ +P Y ILK++ ++++ Sbjct: 332 TLKIHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQL 376 >SP1505 [R] COG0628 Predicted permease Length = 388 Score = 164 bits (414), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 98/337 (29%), Positives = 177/337 (52%), Gaps = 20/337 (5%) Query: 24 SKVVFLITPLVAIFNLLLVPMMLSGFMYYLLRPLVNFLESKKLNRMLSILLIYVVFIGLF 83 SK+ FL +P++ ++++P++LSG +YYLL P+V+++E K+NR+++I +++V+ I LF Sbjct: 40 SKISFLFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVI-IALF 98 Query: 84 VLFWVLVW-PTLREQIQNFIDNTPYLVQGIQEQFNKL-----------QSDPYFSRFFKG 131 +++ + V P L+ Q+ F N P ++ I N L Q + + F Sbjct: 99 IIWGLAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQ 158 Query: 132 DADLTARISGYLSDTITWVTNSMSNLXXXXXXXXXXXATLPIILYYMLKDGHKLSPILLS 191 L +++S S + WV+ +S +P +L+Y+L+DG L L Sbjct: 159 ATVLASKVS---SQAVNWVSAFISGASQVIVALII----VPFMLFYLLRDGKGLRNYLTQ 211 Query: 192 LVPRKYRKEGQDMLQDIDSALSGFIVTRVLLNVVLGAMLYVGFLIIGLPYSLMLAIISIP 251 +PRK ++ +L D++ LS ++ +V + +++ M + F IIGL Y++ L + + Sbjct: 212 FIPRKLKEPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGI 271 Query: 252 LNFIPYVGSLLAAVPVVIVGFIESPTMAIWSXXXXXXXXXXXDNVLSPIIYGKSLDVHPX 311 LN +PY+GS LA +P +++G I P M + +SP+I G L++HP Sbjct: 272 LNLVPYLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPI 331 Query: 312 XXXXXXXXGGDFYGIIGVLIALPVYMILKIIFLRIYE 348 G +GI GVL+ +PVY K++ I+E Sbjct: 332 NVLFVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFE 368 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.148 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,678,789 Number of extensions: 895486 Number of successful extensions: 3281 Number of sequences better than 1.0e-05: 86 Number of HSP's gapped: 3127 Number of HSP's successfully gapped: 86 Length of query: 366 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 258 Effective length of database: 25,922,359 Effective search space: 6687968622 Effective search space used: 6687968622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 116 (49.3 bits)