BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0010.p (294 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4553 [G] COG1175 ABC-type sugar transport systems, permease c... 183 3e-46 TM0278 [G] COG1175 ABC-type sugar transport systems, permease co... 159 7e-39 BH1245 [G] COG1175 ABC-type sugar transport systems, permease co... 156 3e-38 YPO1894 [G] COG1175 ABC-type sugar transport systems, permease c... 153 3e-37 SMa1339 [G] COG1175 ABC-type sugar transport systems, permease c... 136 3e-32 >AGc4553 [G] COG1175 ABC-type sugar transport systems, permease components Length = 312 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 104/298 (34%), Positives = 179/298 (60%), Gaps = 11/298 (3%) Query: 3 RTYFQKNPHLA-----YIMPAMIILGTLTFIPTLFLYVLSVSDYELGYPAFQFVGLDNFI 57 R + P LA +++PA++++ + P +F +SV + LG F+G DN+I Sbjct: 15 RAEGSRPPRLAPNYWPFVIPALVVISAVIVFPWVFTLWMSVHRWTLGQEQ-SFIGFDNYI 73 Query: 58 RLFSGGDAEFWYSVTISLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMT 117 RL S D FW S+ +L + + V L LG A++ D +F L+ + ++P+ T Sbjct: 74 RLAS--DLRFWESLWHTLIYTVLSVVAPLFLGTLAALVFDAQFPLRGFLRGVFVMPMMAT 131 Query: 118 PSITGQVWKLMMNAESGVLNYLLHFIG-GKVIWLSGENA-FWSTVLVDVWQNTPFVALII 175 P VW +M + + GVLNYLL IG G + W+ ++ S VLV+ WQ TP V LI+ Sbjct: 132 PVAIALVWTMMFHPQLGVLNYLLSLIGIGPLEWIYNQSTVIPSLVLVETWQWTPLVMLIV 191 Query: 176 YAGIRSLPTDPYEAAAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLT 235 G+ ++P +PYE+A IDGA+ Q FR +T+P++ P +++AVI R+ID++K+FDI Y++T Sbjct: 192 LGGLAAVPREPYESAEIDGANAWQKFRYLTMPMIAPFLMIAVIIRSIDAVKSFDIIYAMT 251 Query: 236 QGGPGSATEFLSLHVYRLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKGVENK 293 QGGPG+A+E +++++Y F+ +G +A++V+ ++ +S +L+ ++ N+ Sbjct: 252 QGGPGTASETINIYLYNTAFSYYD-IGYGSAMAVVFFVIIVALSFVLLMVRQRSQWNE 308 >TM0278 [G] COG1175 ABC-type sugar transport systems, permease components Length = 294 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 96/267 (35%), Positives = 165/267 (61%), Gaps = 12/267 (4%) Query: 15 IMPAMIILGTLTFIPTLFLYVLSVSDYELGYPA-FQFVGLDNFIRLFSGGDAEFWYSVTI 73 ++P + + +T P F+ S DY+L A F+GL N+ ++F D+ S+ Sbjct: 11 VLPVLALFLAMTIYPFGFMIWASFRDYDLSKSAETHFIGLSNYFQIFR--DSTAIESMKF 68 Query: 74 SLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMTPSITGQVWKLMMNAES 133 + + I +EL+LG +A L+ R K + ++ + +++P+ + P+++G WK++ N Sbjct: 69 TAKLLSIAVPVELVLGVLIAFLI-RGVKGEKIIRSSLLIPMMIPPAVSGVAWKMLYNFAF 127 Query: 134 GVLNYLLHFIG-GKVIWLSGENAFWST---VLVDVWQNTPFVALIIYAGIRSLPTDPYEA 189 G +N+ L G K+ WL + F++ +++DVWQ TPF+ L+IYAG++S+P D +A Sbjct: 128 GPVNWFLSLFGVPKISWLG--DPFYAQLGIIIIDVWQWTPFIFLMIYAGLQSIPQDLIDA 185 Query: 190 AAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEFLSLH 249 A +DGA ++F ++ PLL P+IL+A++ R ID LKTFDI + T GGPGSAT S + Sbjct: 186 ARVDGADWGRVFLHVEFPLLRPLILVALVLRIIDCLKTFDIVFMTTWGGPGSATHTYSFY 245 Query: 250 VYRLGFAQTGW-VGRSAAVSVILLILT 275 +Y++G + GW +G ++A+SV+LLI+ Sbjct: 246 IYKVGIS-FGWNIGYASALSVLLLIVA 271 >BH1245 [G] COG1175 ABC-type sugar transport systems, permease components Length = 445 Score = 156 bits (395), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 82/240 (34%), Positives = 147/240 (61%), Gaps = 7/240 (2%) Query: 49 QFVGLDNFIRLFSGGDAEFWYSVTISLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFA 108 F+G +++ D+ W +++ +L F + EL+LG +A+L++R FK + LV Sbjct: 195 NFIGFSHYVNYVQ--DSRMWAAMSNTLFFTVVSVAAELVLGLAIALLINRTFKGRGLVRV 252 Query: 109 CMIVPIAMTPSITGQVWKLMMNAESGVLNYLLHFIG----GKVIWLSGENAFWSTVLVDV 164 +++P A+ ++ +W+ + + + GV+ + +G V+ +G A +S + DV Sbjct: 253 AVLIPWAIPTAVAAMMWRYLYDGQFGVIAHYFEQLGLISDSSVLLSTGAGAMFSIIFADV 312 Query: 165 WQNTPFVALIIYAGIRSLPTDPYEAAAIDGASRVQIFRNITLPLLWPMILLAVIFRAIDS 224 W+ TP++AL++ AG++++P YEAA +DGA+R+Q F ITLPLL IL+A++FR +D+ Sbjct: 313 WKTTPYMALLLLAGLQTIPNSLYEAAKVDGANRIQTFFKITLPLLKSAILVALLFRTLDA 372 Query: 225 LKTFDIPYSLTQGGPGSATEFLSLHVYRLGFAQTGWVGRSAAVSVILLILTTGISMLLIK 284 + FD+ + LT GGP ++TE +S++ YR F+Q + G +A+SVI+ + IS IK Sbjct: 373 FRVFDLIFVLTGGGPANSTESISIYAYRTLFSQQNF-GAGSALSVIVFLSVALISFAFIK 431 >YPO1894 [G] COG1175 ABC-type sugar transport systems, permease components Length = 286 Score = 153 bits (387), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 93/261 (35%), Positives = 157/261 (60%), Gaps = 9/261 (3%) Query: 36 LSVSDYEL-GYPAFQFVGLDNFIRLFSGGDAEFWYSVTISLGFMGIVTVIELLLGFWLAV 94 LSV D +L P F F+G+ NFI + + W S+ +L ++ TV EL+LG +A+ Sbjct: 32 LSVHDIDLMSPPPFDFIGMANFIEVLQ--EPRMWSSLWNTLVYVAGSTVAELVLGSAIAL 89 Query: 95 LLDREFKLKFLVFACMIVPIAMTPSITGQVWKLMMNAESGVLNYLLHFIG-GKVIWLSGE 153 + R+F + LV A +++P+ +TP + G +W++ + +G+ N+L IG + WL Sbjct: 90 FISRDFFGRKLVRALLLLPMIVTPIVAGLIWRIFYDPNAGLFNWLAGLIGIPPIDWLGNP 149 Query: 154 N-AFWSTVLVDVWQNTPFVALIIYAGIRSLPTDPYEAAAIDGASRVQIFRNITLPLLWPM 212 + A S +L D+WQ TPF+ L++ AG+ +LP++P EAA +DGA ++ I LP++ P+ Sbjct: 150 STAMASLILADIWQWTPFIILLVSAGLDALPSEPLEAAELDGARGWRLLAFIKLPMMKPI 209 Query: 213 ILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEFLSLHVYRLGFAQTGWVGRSAAVSVILL 272 IL+A+ R ID+ K+FD+ Y +T+GGP ATE +++ Y GF R +A VI + Sbjct: 210 ILMALFLRMIDAFKSFDLIYVMTRGGPALATETTNMYAYLTGFQDF----RISAAVVIAI 265 Query: 273 ILTTGISMLLIKAYRKGVENK 293 I T + +L A +K ++++ Sbjct: 266 INTLLVIAVLSFASKKVMKDE 286 >SMa1339 [G] COG1175 ABC-type sugar transport systems, permease components Length = 316 Score = 136 bits (343), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 90/281 (32%), Positives = 157/281 (55%), Gaps = 10/281 (3%) Query: 14 YIMPAMIILGTLTFIPTLFLYVLSVSDYELGYPA-FQFVGLDNFIRLFSGGDAEFWYSVT 72 +I+P ++ L P ++ S +D+ A FVGL N+ L + D W + Sbjct: 44 FIIPTILFLIVFNIFPLIYSLGYSFTDFRASTNAPATFVGLQNYRELLN--DPFIWANFA 101 Query: 73 ISLGFMGIVTVIELLLGFWLAVLLDREFKLKFLVFACMIVPIAMTPSITGQVWKLMMNAE 132 I+ ++ + ++++GF A+LL+RE K L+ +++P+ ++ ++ G WKL+ + Sbjct: 102 ITAKYVIVSVTGQVVVGFGTAMLLNREIPFKGLITTLLLLPMMLSMAVVGLFWKLLYDPS 161 Query: 133 SGVLNYLLHFIGGKVIWLSG-ENAFWSTVLVDVWQNTPFVALIIYAGIRSLPTDPYEAAA 191 G++NY L G WL+ E A ++ + D+W +PFV L+ AG+ ++P YEAAA Sbjct: 162 FGIINYALGL--GSFEWLANPEMALYAVAITDIWMWSPFVMLLSLAGLSAVPRHLYEAAA 219 Query: 192 IDGASRVQIFRNITLPLLWPMILLAVIFRAIDSLKTFDIPYSLTQGGPGSATEFLSLHVY 251 ID A F ITLPL+ P++++A+IFR +++ KTFD+ Y LT TE +S+ +Y Sbjct: 220 IDRAGPFYTFFRITLPLVAPILMIAIIFRTMEAFKTFDLAYILTS---QPTTEVISIRLY 276 Query: 252 RLGFAQTGWVGRSAAVSVILLILTTGISMLLIKAYRKGVEN 292 ++ F Q GRS A++ I+LI+ I+ + +K + E Sbjct: 277 KMAF-QEWQTGRSCALAYIVLIMVLAITNIYVKYLNRVKER 316 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.144 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,374,021 Number of extensions: 956209 Number of successful extensions: 3461 Number of sequences better than 1.0e-05: 206 Number of HSP's gapped: 3285 Number of HSP's successfully gapped: 209 Length of query: 294 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 188 Effective length of database: 26,153,027 Effective search space: 4916769076 Effective search space used: 4916769076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)