BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0495.0005.p
         (274 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DR0599 [V] COG2746 Aminoglycoside N3'-acetyltransferase               192   4e-49
DR2034 [V] COG2746 Aminoglycoside N3'-acetyltransferase                82   7e-16
Cj1298 [V] COG2746 Aminoglycoside N3'-acetyltransferase                75   1e-13
Cj1296 [V] COG2746 Aminoglycoside N3'-acetyltransferase                50   5e-06

>DR0599 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 279

 Score =  192 bits (489), Expect = 4e-49,   Method: Compositional matrix adjust.
 Identities = 101/248 (40%), Positives = 146/248 (58%), Gaps = 4/248 (1%)

Query: 19  TKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGEEGTLMMPSQTW 78
           T+  L     S G+  G  +LVH+SLS+ G++ GGA   I+AL +++  +GTL+MP+ + 
Sbjct: 21  TRASLAADLRSLGVQAGDVLLVHSSLSRLGWVAGGAVAVIQALQDVLTPQGTLVMPTHSS 80

Query: 79  KNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSDHPARS 138
              DP+  +       WW +IR   PA+D  +TP  GMG + E+FR WPG  RSDHP  S
Sbjct: 81  DLTDPAGWLSPPVPEAWWEVIRAELPAFDPALTPTRGMGRIPELFRTWPGVRRSDHPHSS 140

Query: 139 VAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTSLHLAEARADFP 198
            AA G+HAE++T  H L+   G GSPL ++Y LNG VLL  +G ++NT+LHLAE RA   
Sbjct: 141 FAAWGRHAEFVTAGHALAFSLGEGSPLARIYDLNGRVLL--LGTERNTNLHLAEVRAGV- 197

Query: 199 AKKRVYDSSAVMVEGRRKWVTYETQAVNDEDFIRLGEEYDKEMNLKVHQAGKAEIRFMEQ 258
            +  V  S  VMV+G R+W+T++    + E F  +   ++    +     G A+ R M Q
Sbjct: 198 -RPTVAVSGPVMVDGERQWLTFDEPDYDAEAFAPVKAAFEAAGGVVRGTVGSAQARLMSQ 256

Query: 259 RPLVDWAT 266
           R LVD+A 
Sbjct: 257 RELVDFAV 264


>DR2034 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 258

 Score = 82.4 bits (202), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 75/263 (28%), Positives = 110/263 (41%), Gaps = 47/263 (17%)

Query: 18  FTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLEIVGEEGTLMMPSQT 77
            T  DL +   S GL   Q+++VH SL  FG + GGA T +    E+     TL+ P+ T
Sbjct: 1   MTAADLDRGLDSLGLDGTQNVIVHASLRSFGQLEGGARTVVD---ELERRTSTLVAPAFT 57

Query: 78  WKNL--DPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSDHP 135
           +  L   PS+ V                 +++++   +  +G V +   +   A RS HP
Sbjct: 58  YATLLSRPSSPVRA---------------SFNRDSRVSRDIGRVPQEMVERAAARRSFHP 102

Query: 136 ARSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNGYVLLAGVGHDKNTSLHLAEARA 195
           A S  A+G+ AE +T    L + +    P+  LY L+GY LL GV    NT++H  E  A
Sbjct: 103 ALSFVALGQEAERVTAAQTLDSPY---QPIGALYDLDGYALLMGVDFGSNTTVHYGEHLA 159

Query: 196 DFPAKKRVYDSSAVMVEGRRKWVTYETQAV------NDEDFIRLGEEYDKEMNLKVHQAG 249
             P   R              WV  + Q           DF  L E Y +   ++V   G
Sbjct: 160 GMPLLTR--------------WVPLDGQVSPTAFPNCSADFANL-EPYVRGREVQV---G 201

Query: 250 KAEIRFMEQRPLVDWATAWMEKN 272
            A +R    R LVD     +  N
Sbjct: 202 PATLRLYRVRDLVDETVRLLSHN 224


>Cj1298 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 263

 Score = 75.1 bits (183), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 56/184 (30%), Positives = 80/184 (43%), Gaps = 31/184 (16%)

Query: 18  FTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALL----EIVGEEGTLMM 73
           ++  D I+ F   GL KG  + VHT L  FGF +     F++ +L    E++G+EGTL+M
Sbjct: 12  YSTYDFIETFYKLGLQKGDTLCVHTELFNFGFPLLSRNEFLQTILDCFFEVIGKEGTLIM 71

Query: 74  PSQTWKNLDPSTGVHWEESAEWWPMIRQHWPAYDKEITPAIGMGVVAEMFRKWPGAARSD 133
           P+ T+                           YDK I     MG + E FRK  G  R++
Sbjct: 72  PTFTYSFCKNE--------------------VYDK-INSKTKMGALNEYFRKQTGVKRTN 110

Query: 134 HPARSVAAVGKHAEYLTQNHDLSNIFGIGSPLDKLYHLNG-YVLLAGVGHDKNTSLHLAE 192
            P  S A  G   E   +  D ++ FG     + L   NG Y+   G GH   T  H AE
Sbjct: 111 DPIFSFAIKGAKEELFLK--DTTSCFGENCVYEVLTKENGKYMTFGGQGH---TLTHYAE 165

Query: 193 ARAD 196
            + +
Sbjct: 166 EQFN 169


>Cj1296 [V] COG2746 Aminoglycoside N3'-acetyltransferase
          Length = 119

 Score = 49.7 bits (117), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 25/65 (38%), Positives = 40/65 (61%), Gaps = 4/65 (6%)

Query: 18 FTKEDLIKQFTSCGLTKGQHILVHTSLSKFGFIIGGAETFIRALLE----IVGEEGTLMM 73
          ++ +DLI  F   G+ +G  + VHT L KFG  +     F++ LLE    ++G+EGTL+M
Sbjct: 12 YSDKDLIDAFYQLGIKRGDILCVHTELMKFGKALLTKNDFLKTLLECFFKVLGKEGTLLM 71

Query: 74 PSQTW 78
          P+ T+
Sbjct: 72 PTFTY 76


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.319    0.133    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 21,517,011
Number of extensions: 925887
Number of successful extensions: 1804
Number of sequences better than 1.0e-05: 4
Number of HSP's gapped: 1797
Number of HSP's successfully gapped: 4
Length of query: 274
Length of database: 38,378,431
Length adjustment: 105
Effective length of query: 169
Effective length of database: 26,268,361
Effective search space: 4439353009
Effective search space used: 4439353009
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 115 (48.9 bits)