BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0494.0003.p (343 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy1562 [L] COG1518 Uncharacterized protein predicted to be invo... 314 1e-85 alr1568 [L] COG1518 Uncharacterized protein predicted to be invo... 199 5e-51 alr1468_2 [L] COG1518 Uncharacterized protein predicted to be in... 167 2e-41 alr0381 [L] COG1518 Uncharacterized protein predicted to be invo... 135 8e-32 AF2435 [L] COG1518 Uncharacterized protein predicted to be invol... 116 6e-26 >SPy1562 [L] COG1518 Uncharacterized protein predicted to be involved in DNA repair Length = 673 Score = 314 bits (805), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 22/341 (6%), Positives = 62/341 (18%), Gaps = 1/341 (0%) Query: 1 MKKLLNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYC 60 M+ + L K + + A Sbjct: 1 MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAA 60 Query: 61 ADSNISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHK 120 + + + + K+ + ++ + + Sbjct: 61 REMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGM-KANFSLFDDTDQLALLKE 119 Query: 121 WMLERMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQM 180 + D L + + + A +R R Sbjct: 120 LTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYDAHLKACN 179 Query: 181 ILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGR 240 +L + + + + + LL + + + R R Sbjct: 180 VLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTV 239 Query: 241 VSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKK 300 V + RG VL + + + + + A + Sbjct: 240 VGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFE 299 Query: 301 QETITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFL 341 + + G ++ + Sbjct: 300 KRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKT 340 >alr1568 [L] COG1518 Uncharacterized protein predicted to be involved in DNA repair Length = 673 Score = 199 bits (506), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 18/340 (5%), Positives = 51/340 (15%), Gaps = 8/340 (2%) Query: 5 LNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYCADSN 64 + Q +V V+ K I GY A + + + Sbjct: 1 MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAA 60 Query: 65 ISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHKWMLE 124 + G E K + Sbjct: 61 REMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKEL 120 Query: 125 RMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQMILQQ 184 +V ++ + + G+ + + Sbjct: 121 TEGLIEDDKVLLQQ-----LISTISNWKNDLKTPSQAAASAIGERDRIFAHCYGLYD--A 173 Query: 185 KEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGRVSLA 244 L + + V + + + Sbjct: 174 HLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGS 233 Query: 245 LDVMEELRGVFADKFVLTLINKKLISKADFLKKE-NGAVIMTDEARKKFLAAWQSKKQET 303 + + + + + + + + Sbjct: 234 RARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIAN 293 Query: 304 ITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 H + S ++ + L Sbjct: 294 NPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIA 333 >alr1468_2 [L] COG1518 Uncharacterized protein predicted to be involved in DNA repair Length = 673 Score = 167 bits (424), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 17/340 (5%), Positives = 45/340 (13%), Gaps = 8/340 (2%) Query: 5 LNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYCADSN 64 Q +++ + + A+ + Sbjct: 2 RLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAR 61 Query: 65 ISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHKWMLE 124 + L + F L Sbjct: 62 EMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMKANFSLFDDTDQLALLKELT 121 Query: 125 RMTRDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQMILQQ 184 + + +L S + L Q E + Sbjct: 122 EGLIEDDKVLLQQLISTISNWKNDLKT----PSQAAASAIGERDRIFAHCYGLYDAH--- 174 Query: 185 KEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGRVSLA 244 + L + + + L + V Sbjct: 175 LKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSR 234 Query: 245 LDVMEELRGVFADKFVLTLINKKLISKADFLKK-ENGAVIMTDEARKKFLAAWQSKKQET 303 + + L+ + + + + + L A Sbjct: 235 ARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANN 294 Query: 304 ITHPYLGEKISWGLVPXXXXXXXXXXXXNDLDEYPPFLWK 343 G + Sbjct: 295 PHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH 334 >alr0381 [L] COG1518 Uncharacterized protein predicted to be involved in DNA repair Length = 673 Score = 135 bits (341), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 25/294 (8%), Positives = 60/294 (20%), Gaps = 14/294 (4%) Query: 5 LNTLYITQPSVYLSLDGDNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYCADSN 64 L Q ++ N + + + L E+ T + L + Sbjct: 39 LIRGCGYQARHIAAVTFTNKAAREMK-ERVGQTLGRKEARGLMISTFHTLGLDIIKREYA 97 Query: 65 ISLLFLTMNGRYLARVTGQSRGNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHKWMLE 124 + + + + + ++ S I+ K + Sbjct: 98 ALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLIST-ISNWKNDLKTPSQAAASA 156 Query: 125 RMTRDYPLRVN-----VELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQ 179 RD L LIL E + ++ Sbjct: 157 IGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLL----QANEEVRKRWQNKIRYLL 212 Query: 180 MILQQKEDFYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPG 239 + Q S+ L + S A + + Sbjct: 213 VDEYQ---DTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALK 269 Query: 240 RVSLALDVMEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFL 293 + L + R + A ++ + + + ++ Sbjct: 270 VIKLEQNYRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHE 323 >AF2435 [L] COG1518 Uncharacterized protein predicted to be involved in DNA repair Length = 673 Score = 116 bits (290), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 26/308 (8%), Positives = 52/308 (16%), Gaps = 28/308 (9%) Query: 16 YLSLDG-----DNVVLLKEEEKLGRFPLHNLESIVTFGYTGASPALMGYCADSNISLLFL 70 V+ K I GY A + + + + Sbjct: 7 QQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKER 66 Query: 71 TMNGRYLARVTGQSR---GNVVLRKKQYNLSEDEVRSAKIARNFIIGKIYNHKWMLERMT 127 G + L + + F L Sbjct: 67 VGQTLGRKEARGLMISTFHTLGLDIIKREY--AALGMKANFSLFDDTDQLALLKELTEGL 124 Query: 128 RDYPLRVNVELFKETSQQLSSLILEVRACEQLDRLRGLEGQAAATYYSQFNQMILQQKED 187 + + L L + L+ AA+ + Sbjct: 125 IE------------DDKVLLQQ-LISTISNWKNDLKTPSQAAASAIGERDRIFA----HC 167 Query: 188 FYFHSRTRRPPLDNVNAMLSLAYTLLTHDTASALEAVGLDAYVGFLHRDRPGRVSLALDV 247 + + + L L TLL + + + S V Sbjct: 168 YGLYDAHLKACNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELV 227 Query: 248 MEELRGVFADKFVLTLINKKLISKADFLKKENGAVIMTDEARKKFLAAWQSKKQETITHP 307 + V + + + A K + I Sbjct: 228 KLLVGSRARFTVVGDDDQSIYSWRGAR-PQNLVLLSQDFPALKVIKLEQNYRSSGRILKA 286 Query: 308 YLGEKISW 315 + Sbjct: 287 ANILIANN 294 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,382,173 Number of extensions: 920411 Number of successful extensions: 1639 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 1618 Number of HSP's successfully gapped: 12 Length of query: 343 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 236 Effective length of database: 26,037,693 Effective search space: 6144895548 Effective search space used: 6144895548 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)