BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0493.0029.p
         (639 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CAC0355 [M] COG5434 Endopolygalacturonase                              71   6e-12

>CAC0355 [M] COG5434 Endopolygalacturonase
          Length = 513

 Score = 70.9 bits (172), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 65/212 (30%), Positives = 94/212 (44%), Gaps = 38/212 (17%)

Query: 172 VYVPAGVYKSGNLVLKSNVSVYLEGGSVIRGSGNPSDYTTHFHKNSL-NKD---GTWF-- 225
           V +P G Y +G L LKSN+++ LE GSV+ G      Y       S+ N+D   G+W   
Sbjct: 116 VIIPKGTYLTGPLFLKSNITLELEEGSVLLGLKEREHYPILKANISMTNRDFYLGSWEGN 175

Query: 226 -------IYTETNANNIKIYGRGTIDGNGHY-------------MRNTNNYLNNILVPLQ 265
                  + T  N  N+ I G+GTIDGN  +              R    +LN       
Sbjct: 176 EADCFASLITAINVENVNIIGKGTIDGNSDFDTWWFKAKEKRIAWRPRTLFLN------A 229

Query: 266 CSNFTVDGITIRDSGGWATIVTRSNNVTFQNTKHFNNNELDYEN-DAIDIQESQNVLIKH 324
           C N  V+G+TI++S  W      S+++ F N      N  +  N DA+D +  +NVLI  
Sbjct: 230 CKNILVEGVTIKNSPSWTIHPLMSDHLKFINLSI--ENPFNAPNTDALDPESCKNVLILG 287

Query: 325 TVAVSEDDTYSFKTWDVATTDIAANWPGSPEN 356
                 DD  + K+  +   DI+   P S EN
Sbjct: 288 DTFSVGDDCIAIKSGKI---DISKKNPVSSEN 316


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.314    0.130    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 43,155,783
Number of extensions: 1772680
Number of successful extensions: 3423
Number of sequences better than 1.0e-05: 1
Number of HSP's gapped: 3423
Number of HSP's successfully gapped: 1
Length of query: 639
Length of database: 38,378,431
Length adjustment: 113
Effective length of query: 526
Effective length of database: 25,345,689
Effective search space: 13331832414
Effective search space used: 13331832414
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 119 (50.4 bits)