BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0023.p (531 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1123 [T] COG4753 Response regulator containing CheY-like recei... 149 2e-35 SPy1587 [T] COG4753 Response regulator containing CheY-like rece... 144 5e-34 BH2109 [T] COG4753 Response regulator containing CheY-like recei... 132 1e-30 BH3842 [T] COG4753 Response regulator containing CheY-like recei... 127 5e-29 BH2728 [T] COG4753 Response regulator containing CheY-like recei... 96 2e-19 >BH1123 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 526 Score = 149 bits (375), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 131/537 (24%), Positives = 248/537 (46%), Gaps = 37/537 (6%) Query: 1 MIVDDEPVIKKGLKCFVDWATLQCEVICEASNGIEAVEMLGHNDIDIIVTDIRMPGMDGL 60 +I+DDE + +GLK + W L CE + EA+NG ++++ I++TDI MP M GL Sbjct: 5 VIIDDEEHVVRGLKKIISWKALGCECVGEAANGRRGLDIVRETSPHIVLTDIYMPIMYGL 64 Query: 61 ALSDYVHQNFPQIKVIILTAFADFSYAQTAIQYEVVDFVVKTNPTEKIPGAIEKATRLLE 120 + + ++ +V+IL+ +++F +A+ A+Q + D++ K + I +++ LE Sbjct: 65 DMIQILREDGFDGEVVILSGYSEFEHARKAMQLNITDYLSKPASVDTIETVLKRVINKLE 124 Query: 121 KENEQKQKLRQLESKINDHLSEISEKFLKEAAHGLISD----EASLLSRSRELGLQLENY 176 ++ Q+ L++L +K+ + + +++K G + + E L ++ Q Sbjct: 125 EKQRQQGYLQELTNKVKRYEPLVETEWMKSLLTGRLRNGEIPEEFLAKITKWKDQQ---- 180 Query: 177 FGVHLEVEDTLGSPPTNE-----YHRFLASIRQFLALAFEERPTYIMPMEKNTLLAIVSM 231 H+ T P E ++ F S+ + + P +E + + + + Sbjct: 181 ---HIACSLTYDGPQNKELKPADWYLFRYSVHKMIREVLNGYPIDFRYLEMDAYRSALCL 237 Query: 232 GDGNAAVSTQALLTICNEILAMAENFRRYHLNIGISL----MHRDVQTLSMAYREAREAL 287 N +VS L + EI E N+ ++ + D LS ++ EA Sbjct: 238 -HVNKSVSEPVLSCMLMEIKDQVEQGIYSSFNVKTTVHYGRVKEDWTDLSESFHEACMES 296 Query: 288 Q----GSFYSDNYVAVYMPHTSQTLTPGAPPHHTAERIAEFLQQGQNDLAIDQLERLLES 343 Q G+ Y D A + +SQ L A + A R E L++ L L+ Sbjct: 297 QSFPLGNPYEDGTRATFPMESSQKLYD-AIRYADATRAKEVLEEAVGVLGEVPYSTLV-G 354 Query: 344 YRNIKEPIENVKVACLLIGSYCFRLLSASHPFAPELDESQSAVYKQIQESKSIQLLADIL 403 Y+ E + + IG + F + D +Q KQ + I+ L + + Sbjct: 355 YKVGIEIWTIITYSLYDIG------ILIQEMFDEDFDLAQHLT-KQGNWQEWIRFLCEQI 407 Query: 404 KRLIRSCSRAVAFNDKQPNYIVIECQKYIKEHYNQNLNLQIIADHIHINSSYLSRLYKKV 463 + + C + + ++ + YI++H +N+ LQ IAD ++I+ +YL +++KKV Sbjct: 408 EHI---CYHQQWDENIKHKQLIEQVMIYIEKHLAENITLQDIADELYISRNYLGQIFKKV 464 Query: 464 TNESIIDAINKYRIEMAKKLLRNPASKVFEVAEAVGIETPAYFTHVFSKYTGMSPKE 520 ES + + + R+E AKK+++ ++EV+E VG PAYFT +F KYTG +P++ Sbjct: 465 VGESFKNYVTRLRMEKAKKMIQEGNFLIYEVSEKVGYVNPAYFTTMFKKYTGYTPRD 521 >SPy1587 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 494 Score = 144 bits (362), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 130/532 (24%), Positives = 241/532 (45%), Gaps = 60/532 (11%) Query: 1 MIVDDEPVIKKGLKCFVDWATLQCEVICEASNGIEAVEMLGHNDIDIIVTDIRMPGMDGL 60 ++VDDE +I +GL +DW L EV+ A +G EA+ L +D++++D+ MPGM GL Sbjct: 5 LLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYLTQYPVDVMISDVTMPGMTGL 64 Query: 61 ALSDYVHQNFPQIKVIILTAFADFSYAQTAIQYEVVDFVVKTNPTEKIPGAIEKATRLLE 120 L + PQ++ +IL+ + +FSY Q A++ E +++K ++ +++ L+ Sbjct: 65 DLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGYLLKPVDKAELQAKMKQFKDWLD 124 Query: 121 KENEQKQKLRQLESKINDHL------SEISEKFLKEAAHGLISDEASLLSRSRELGLQLE 174 + Q + +RQ +D L E+SEK ++ + GL + L Sbjct: 125 AQ--QAESIRQ--EAYHDSLLTLWLTDELSEKEFQQLSQGLPA-------------AALT 167 Query: 175 NYFGVHLEVEDTLGSPPTNEYHRFLASIRQFLALAFEERPTYIMPMEKNTLLAIVSMGDG 234 + ++L+ ++ + A+I + E +P Y+ E + V +G Sbjct: 168 GFTVLYLDCQE------------WQAAIDAYFER--EGQPFYLKKEEDKKVYLAVLLGKT 213 Query: 235 NAAVSTQALLTICNEILAMAENFRRYHLNIGISLMHRDVQTLSMAYREAREALQGSFYSD 294 + A + L +R+ N+ ++ V Y + L+ FY+ Sbjct: 214 DQAKAFTYDLQ------------KRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNC 261 Query: 295 NYVAVYMPHTSQTLTPGAPPHHTAERIAEFLQQGQNDLAIDQLERLLESYRNIKEPIENV 354 + S+ P + A A L G + +LE + + + + E+V Sbjct: 262 EVSPIRAKDKSKIELPESRLQFFAFNKA--LMIGDEPTILSKLEAIFDEMKTLNFSPEDV 319 Query: 355 KVACLLIGSYCFRLLSASHPFAPELDE-SQSAVYKQIQESKSIQLLADILKRLIRSCSRA 413 K L+ S +R P LD+ + ++ K I +S+SI + LK+++ ++ Sbjct: 320 KHVSFLLFSDIYRQF-------PILDKMTYLSMVKTIHDSQSIDCILRELKKVLDVTNQN 372 Query: 414 VAFNDKQPNYIVIECQKYIKEHYNQNLNLQIIADHIHINSSYLSRLYKKVTNESIIDAIN 473 + +K+ + +V E I++ Y+Q L L+ IAD +H+N YL + +K T S +N Sbjct: 373 NS-PEKRYSDLVSETIDCIRKEYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLN 431 Query: 474 KYRIEMAKKLLRNPASKVFEVAEAVGIETPAYFTHVFSKYTGMSPKEYKLNY 525 RI+ A++LL + E+A G T YF +F K G+SPKE++ Y Sbjct: 432 HVRIQKAQQLLLYTNQSINEIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRY 483 >BH2109 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 525 Score = 132 bits (333), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 134/546 (24%), Positives = 249/546 (45%), Gaps = 54/546 (9%) Query: 1 MIVDDEPVIKKGLKCFVDWATLQCEVICEASNGIEAVEMLGHNDIDIIVTDIRMPGMDGL 60 ++ DDE ++ GLK ++W EV EA NG +A +++ D+++TDIRMP DGL Sbjct: 5 LLADDEFFVRHGLKSLIEWEKCGYEVAAEADNGEDAFKLVQEIKPDLVITDIRMPVFDGL 64 Query: 61 ALSDYVHQNFP-QIKVIILTAFADFSYAQTAIQYEVVDFVVKTNPTEKIPGAIEKATRLL 119 L V++ K II++ + DFSYAQ A+++ VDFV+K +++ A+ K L Sbjct: 65 QLIQKVNETLSFNTKFIIISGYNDFSYAQKAVRFGAVDFVLKPIDQDELEAALIKLAATL 124 Query: 120 EKE---NEQKQKLRQLESKINDHLSEISEKFLKEAAHGLISDEASLLSRSRELGLQLEN- 175 ++E N ++K+ + + F+ H L ++ R L + N Sbjct: 125 DQEKATNSDQEKVL------------LDQIFI----HSLGENKKENEVRRWAAKLNINNR 168 Query: 176 --YFGVHLEVED-TLGSPPTNEYHRFLASIRQFLALAFEERPTYIMPMEKNTLLAIVSMG 232 Y+ + LEV + +L NE ++ IR+ + R T AI Sbjct: 169 HFYYYLLLEVNNLSLDKGFINEQYK--EPIRKVI------RETVARQAATEEYTAIYEQL 220 Query: 233 DGNAA--VSTQALLTICNEILAMAENFRR-------YHLNIGISLMHRDVQTLSMAYREA 283 D ++++ L +I +AE R+ L + + D+ T+ +Y E Sbjct: 221 DHAYGLLITSEHLKKFNGQIEPLAEKLRKDIQDFTNIPLTVYVGAKTDDLSTIKKSY-ET 279 Query: 284 REALQGSFYSDNYVAVYMPHTSQTL--TPGAPPHHTAERIAEFLQQGQNDLAIDQLERLL 341 +L+ Y N V++ ++++ T +H + E +++ +D ++ ++++ Sbjct: 280 ANSLRNYKYIFNEKNVFIHEQTESVDTTYDELNNHLFVTLMEQMEENNSDRIVNIIDKIF 339 Query: 342 ESYRNIKEPIENVKVACLLIGSYCFRLLSASHPFAPELDESQSAVYKQIQESKSIQLLAD 401 ++ K VK + + +++ A E D + + + +S L D Sbjct: 340 SEFQTKKFVPSAVKTS---VNRCVHKVMQTVQ--AMEGDTGKIISAQAMTNWESYNLTFD 394 Query: 402 ILKRLIRS--CSRAVAFNDKQPNYI---VIECQKYIKEHYNQNLNLQIIADHIHINSSYL 456 LK++ AV + + + + + + YI+ HY++N++L+ IA ++N Y+ Sbjct: 395 QLKQVFTKFVLEAAVIIQELRKENMKSDIYKVKTYIENHYHENISLKSIASLFYMNPVYM 454 Query: 457 SRLYKKVTNESIIDAINKYRIEMAKKLLRNPASKVFEVAEAVGIETPAYFTHVFSKYTGM 516 +L+KK + + K R+ AKKLLR +V+EVAE VG + YF F K M Sbjct: 455 GQLFKKTFGVYFKEYLLKLRMNEAKKLLRQTDMRVYEVAERVGFGSTDYFVTQFEKINKM 514 Query: 517 SPKEYK 522 +P EY+ Sbjct: 515 TPTEYR 520 >BH3842 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 530 Score = 127 bits (318), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 119/538 (22%), Positives = 252/538 (46%), Gaps = 44/538 (8%) Query: 1 MIVDDEPVIKKGLKCFVDWATLQCEVICEASNGIEAVEMLGHNDIDIIVTDIRMPGMDGL 60 +IVDDE + ++GLK F+ ++ EASNG +A+E D+++ DI+MPG+DG+ Sbjct: 5 LIVDDEQIEREGLKLFLK-DRPNIHIVGEASNGRQAIEKALETQPDLMMMDIKMPGVDGV 63 Query: 61 ALSDYVHQNFPQIKVIILTAFADFSYAQTAIQYEVVDFVVKTNPTEKIPGAIEKATRLLE 120 + Q P+ + I+++AF F YA+ ++Y V ++++K E + A+++ +E Sbjct: 64 QAVKEIKQQLPETEFIMVSAFDTFEYAREVMRYGVKEYLLKPTTKEDVLTALDRIVGEIE 123 Query: 121 KENEQKQKLRQLESKINDHLSEISEKFLKEAAHGLISD---EASLLSRSRELGLQLENY- 176 + E+K + K+ L +IS E+ L+ D + + + L + E Y Sbjct: 124 QAREEKND----QYKLQQRLEQISSFVQTESILYLLMDHIQDYEWMEWNEWLRTEQEGYA 179 Query: 177 --FGVHLEVEDTLGSPPTNEYHRFLASIRQFLALAFEERPTYIMPMEKNTLLAIVSMGDG 234 F H + ++ + E ++ A I++ + L + + + P + L ++ + Sbjct: 180 VVFSFHQQGKEIRKT----EKQQWHARIKKEIGLQLPK--STVGPFIGHHLPILMLTENE 233 Query: 235 NAAVSTQALLTICNEILAMAENFRRYHLNIGISLMHRDVQTLSMAYREAREAL------- 287 N + + + + + + + G+ ++R + S +YREA AL Sbjct: 234 NGMTKQEREQVVIQLLHKVKQAVQPAKVKTGVGGIYR-LHFFSTSYREALLALDIIYDHK 292 Query: 288 QGSFY-SDNYVAVYMPHTSQTLTPGAPPHHTAERIAEFLQQGQNDLAIDQLERLLES-YR 345 Q S+Y D V + + L P ++ E ++ G + A+ + +++ R Sbjct: 293 QASYYFYDKNVKI---RADEQL-----PIERENKLIEAIKSGDGEKALQAFDHYVQAILR 344 Query: 346 NIKEPIENVKVACLLIGSYCFRLLS-ASHPFAPELDESQSAVYKQIQESKSIQLLADILK 404 K P+ +++ + RLL+ + D ++ + Q+ E ++L Sbjct: 345 CSKHPLHDMRKYLQQLFIILGRLLNEMGLHWETAQDFARFSTVHQVTEHGKSEIL----- 399 Query: 405 RLIRSCSRAVAFNDKQPNYIVIECQKYIKEHYNQNLNLQIIADHIHINSSYLSRLYKKVT 464 ++R+ +++ +++ + Y+ +H+ + L L +A HI ++ Y+S+L+K+ Sbjct: 400 HIVRTLQEWRTTDEQS---LMLLARDYVHDHFREPLTLDDLAKHIGLSPYYVSKLFKERF 456 Query: 465 NESIIDAINKYRIEMAKKLLRNPASKVFEVAEAVGIETPAYFTHVFSKYTGMSPKEYK 522 + + + + + R+E AK+LL + E+ +G + P YF+ VF K TG SP +Y+ Sbjct: 457 DLTFSEYVTQLRVEHAKQLLSLTQKSLKEITYEIGYKDPNYFSRVFKKLTGKSPSDYR 514 >BH2728 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 510 Score = 95.5 bits (236), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 40/107 (37%), Positives = 72/107 (67%) Query: 1 MIVDDEPVIKKGLKCFVDWATLQCEVICEASNGIEAVEMLGHNDIDIIVTDIRMPGMDGL 60 +IVDDE +I++G+ F DW +++ EA+NG +A+E++ N+ I++TDI MP MDG+ Sbjct: 5 LIVDDEKLIRQGIINFFDWEKEGFQIVGEAANGKDALELISKNEPHIVMTDIVMPVMDGI 64 Query: 61 ALSDYVHQNFPQIKVIILTAFADFSYAQTAIQYEVVDFVVKTNPTEK 107 L V ++FP++++I+L++F +F Y ++ V D+++K EK Sbjct: 65 DLVKRVKRDFPEVEIIVLSSFEEFEYVRSTFHSGVADYILKPRLNEK 111 Score = 73.9 bits (180), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 39/145 (26%), Positives = 76/145 (52%) Query: 378 ELDESQSAVYKQIQESKSIQLLADILKRLIRSCSRAVAFNDKQPNYIVIECQKYIKEHYN 437 EL+ + + I +K+++ D + + + ++ + + + + YI+EH++ Sbjct: 355 ELEREKYHYFATINAAKNVREATDCFYQFLEVTKQIISVYSNEGSSSIQKMLAYIEEHHS 414 Query: 438 QNLNLQIIADHIHINSSYLSRLYKKVTNESIIDAINKYRIEMAKKLLRNPASKVFEVAEA 497 + L+L +A++ H N SYLS +++ +E + +NK RI AK+ L++ + E+ Sbjct: 415 EPLSLSQLANYFHFNPSYLSAYFRQHHDEGFSEYLNKVRIRKAKEWLKSSTIPISEIYAL 474 Query: 498 VGIETPAYFTHVFSKYTGMSPKEYK 522 VG +YF VF K TGM P Y+ Sbjct: 475 VGYSDHSYFCKVFKKRTGMPPSRYR 499 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,127,614 Number of extensions: 1340441 Number of successful extensions: 6708 Number of sequences better than 1.0e-05: 201 Number of HSP's gapped: 6768 Number of HSP's successfully gapped: 212 Length of query: 531 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 420 Effective length of database: 25,576,357 Effective search space: 10742069940 Effective search space used: 10742069940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)