BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0021.p (449 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 78 4e-14 alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic... 74 4e-13 BH1244 [G] COG1653 ABC-type sugar transport system, periplasmic ... 70 5e-12 AGl1009 [G] COG1653 ABC-type sugar transport system, periplasmic... 66 1e-10 ML1427 [G] COG1653 ABC-type sugar transport system, periplasmic ... 59 2e-08 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 42/159 (26%), Positives = 86/159 (54%), Gaps = 4/159 (2%) Query: 73 EEQIKQFNAKYSNEVEVVVEEIPSDSAYEDKLKILAVSGDVPDVVMG-KNGINDILIKGN 131 EE +K+F + + +++ +E + +DS Y+DK+K++ + PD+ + I+GN Sbjct: 50 EEVVKEFEKDHPD-IDIQIEAVLNDS-YKDKIKVMLGTTSPPDIYFSWSDEFAFKFIRGN 107 Query: 132 LVTPFNDYLDKDPEWKAEVGEAALAANTRDGKVWSISDQKQNIGYFYNKEMFEKAGIKPA 191 + Y D +W +++ ++ + T + K + + Q +FYNK++F+K + P Sbjct: 108 KALDLSSYYKNDTDWSSQLVQSQITPFTYENKQYGVPWQMDAKSFFYNKDIFQKLNLDPP 167 Query: 192 QTWDEFMSNNEKLKAAGFVPLALMTGENAWTTNLILAAL 230 +TWDE + ++KLK G+ P++ T + WT + + L Sbjct: 168 KTWDELIDVSKKLKEHGYTPISFGT-KATWTISHYIGTL 205 >alr2722 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 359 Score = 74.3 bits (181), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 0/344 (0%), Positives = 0/344 (0%), Gaps = 37/344 (10%) Query: 73 EEQIKQFNAKYSNEVEVVVEEIPSDSAYEDKLKIL-AVSGDVP-DVVMGKNGINDILIKG 130 + + D AV + + + + + Sbjct: 65 RLRAGGITKPVL---LLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWM 121 Query: 131 NLVTPFNDYLDKDPEWKAEVGEAALAANTRDGKVWSISDQKQNIGYFYNKEMFEKAGI-K 189 L T + + + V +S + + A Sbjct: 122 KLDTGMHRLGVRPEQ-AEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNT 180 Query: 190 PAQTWDEFMSNNEKL---KAAGFVP----LALMTGENAWTTNLILAALVGTNGEAGNTFM 242 + S ++ + + A G Sbjct: 181 FCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQP----VMS 236 Query: 243 NTLHPTDFNTPEMIQALNMMKTMLEKYTTKDXXXXXXXXXXXXFSQNKAAMIANGPWMIG 302 T + + + Sbjct: 237 LTSSLIAVR-EHKAGEPVGYGGTWVSERDT-RLGVVAMGYGDGYPRAAPSGTPVLVN--- 291 Query: 303 DFSDSTKSSEGFD-KKVGVAAYPNNSLISTYEVGYMIGAK-TPETRDAAEKFIRFKTGIE 360 + V P + G + + + + Sbjct: 292 --GREVPIVGRVAMDMICVDLGPQAQDKA----GDPVILWGEGLPVERIAEMTKVSAYEL 345 Query: 361 GQTIALEYGNVMPVSDK-IQP---SDALKQKYPL--FVESIKLA 398 Sbjct: 346 389 >BH1244 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 70.5 bits (171), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 114/476 (23%), Positives = 178/476 (37%), Gaps = 97/476 (20%) Query: 10 STLILALSVSACGSQNSPENXXXXXXXXXXXXXQANDAPAAKAPVKISYLTFRVGTHASA 69 +TL++++ ++ACG + + +A A V I+Y T + Sbjct: 13 ATLVMSVMLAACGGSDPTDEPAEPTEPGDETEEEAT---ADDEVVTITYARGYDQTGGNE 69 Query: 70 KLEEEQIKQFNAKYSNEVEVVVEEIPSDSAYEDKLKILAVSGDVPDVVMGKNGINDILIK 129 KL I+ F K+ N ++V +E+P+D+ + + A S ++ Sbjct: 70 KL----IEAFMEKHPN-IKVEFQEMPADTGAQHSAYVTAFSAQSSEI------------- 111 Query: 130 GNLVTPFNDYLDKDPEWKAEVGEAALAANT-----RDG----KVWSISDQKQNIGYF-YN 179 D D D W AE +A A RDG + + + Q N+G Y Sbjct: 112 --------DVFDVDVIWPAEFAQANYALELDRFIERDGIDMNQYFPGTVQAGNVGGIQYA 163 Query: 180 KEMFEKAGI---------KPAQTWDEFMSNNEKL------KAAGFVPLALMTGENAWTTN 224 F AG+ P TWDE + E L K + A G T Sbjct: 164 MPKFTDAGLLYYRSDIVETPPSTWDELLEMAEDLQGEEGTKFGYLMQAAQYEGMVVNATE 223 Query: 225 LILA---ALVGTNGEAGNTFMNTLHPTDFNTPEMIQALNMMKTMLEK-YTTKDXXXXXXX 280 I A A++ NGE N+PE I+ L MK +++ D Sbjct: 224 FIAAYGGAVIDENGE-----------VVINSPEAIKGLEKMKEIVQSDVVPSDITNFMET 272 Query: 281 XXXXXFSQNKAAMIANGPWMIGDFSDSTKSSEGFDKKVGVAAYPNNSLISTYEVGYMIGA 340 F Q A N P+M SD +S+ V+ SL+ + G G Sbjct: 273 ETANSFIQGNAVFARNWPYMQA-LSDGDESN--------VSGNVEFSLLPAGDAGNAAGL 323 Query: 341 --------KTPETRDAAEKFIRFKTGIEGQTIALEYGNVMPVSDKIQPSDALKQKYPL-- 390 + E DAA + ++F TG EGQ I +G P + + D + + PL Sbjct: 324 GGWMVMINRYSENVDAAWELVKFMTGPEGQKITAIHGGNAPTIEALYSDDEVLEASPLFA 383 Query: 391 ---FVESIKLAQETQVHYQSFDSIVYPNITDAWKSLYPKLVFGQSTAEEIAKELTE 443 FVE+++ A V S +YP I+D + KL+ G+ TAEE + + E Sbjct: 384 NEEFVETLQNAVPRPV------SPIYPEISDIMQVEISKLLAGELTAEEAVQNMEE 433 >AGl1009 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 134 Score = 65.9 bits (159), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 0/338 (0%), Positives = 0/338 (0%), Gaps = 33/338 (9%) Query: 76 IKQFNAKYSNEVEVVVEEIPSDSAYEDKLKILAVSGDVPDVVMGKNGINDILIKGNLVTP 135 + A ++ V +K G+ ++ + Sbjct: 47 KAKAPA-VLQKLAQAVAAEGFSGIQINKALSSI-DAHQETSGSGRIQTLRVVARQKGAAV 104 Query: 136 FNDYLDKDPEWKAE-VGEAALAANTRDGKVWSISDQKQNIGYFYNKEMFEKAGI---KPA 191 D + + Sbjct: 105 RIDAVFNIQAGQIAD 164 Query: 192 QTWDEFMSNNEKL----KAAGFVPLALMTGENAWTTNLILAALVGTNGEAGNTFMNTLHP 247 Sbjct: 165 ---------- 214 Query: 248 TDFNTPEMIQALNMMKTML-EKYTTKDXXXXXXXXXXXXFSQNKAAMIANGPWMIGDFSD 306 Sbjct: 215 ------ 268 Query: 307 STKSSEGFDKKVGVAAYPNNSLISTYEVGYMIGAKTPETRDAAEKF--IRFKTGIEGQTI 364 Sbjct: 269 - 327 Query: 365 ALEYGNVMPVSDKIQP--SDALK-QKYPLFVESIKLAQ 399 Sbjct: 328 365 >ML1427 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 445 Score = 58.5 bits (140), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 74/311 (23%), Positives = 115/311 (36%), Gaps = 30/311 (9%) Query: 88 EVVVEEIPSDSAYEDKLKILAVSGDVPDVVMGKNGINDILIKGNLVTPFNDYLDKDPEWK 147 ++ V + S +L G PDV+M + L ++ N L +D + Sbjct: 64 DIKVRAVLSGPGVMQQLSTFCAGGKCPDVLMAWDLTYAELADRGVLLDLNTLLGQDKAFA 123 Query: 148 AEVG----EAALAANTRDGKVWSISDQKQNIGYFYNKEMFEKAGIKPAQ-TWDEFMSNNE 202 AE+ E T +G ++ +Q FYNK++F AG++P TW++ S E Sbjct: 124 AELKSDSIEPLYETFTFNGGQYAFPEQWSGNYLFYNKQLFTNAGVQPPPCTWEQPWSFTE 183 Query: 203 KLKAAGFVPLALMTGE-------NAWTTNLILAALVGTNG-EAGNTFMNTLHPTDFNTPE 254 L A + +G N W + NG N MN H +F+ Sbjct: 184 FLDTARALTKRDSSGRVTQWGFVNTWLSYYTAGLFALNNGVPWSNPRMNPTH-LNFDDDA 242 Query: 255 MIQALNMMKTMLEKY-TTKDXXXXXXXXXXXXFSQNKAAMIANGPWMIGDFSDSTKSSEG 313 I+A+ + KY D FS KAA+ G W F +EG Sbjct: 243 FIEAVQFYCDLTNKYQVAPDASEQQWMATADLFSLGKAAIALGGHWRYQTF----MRAEG 298 Query: 314 FDKKV----------GVAAYPNNSLISTYEVGYMIGAKTPETRDAAEKFIRFKTGIEGQT 363 D V V A + + G I A + ++ A +F++F TG GQ Sbjct: 299 LDFDVTSLPIGPSAGTVPATRSGACSDIGATGLAIAASS-SRKEQAWEFVKFATGPAGQA 357 Query: 364 IALEYGNVMPV 374 + E +PV Sbjct: 358 LIGESCLFVPV 368 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.129 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,757,791 Number of extensions: 1214679 Number of successful extensions: 1983 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 2000 Number of HSP's successfully gapped: 17 Length of query: 449 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 339 Effective length of database: 25,691,691 Effective search space: 8709483249 Effective search space used: 8709483249 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)