BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0019.p (245 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurM [G] COG0395 ABC-type sugar transport system, permease co... 168 6e-42 BMEI1714 [G] COG0395 ABC-type sugar transport system, permease c... 157 2e-38 mll0851 [G] COG0395 ABC-type sugar transport system, permease co... 151 9e-37 BH1246 [G] COG0395 ABC-type sugar transport system, permease com... 149 5e-36 Rv1237 [G] COG0395 ABC-type sugar transport system, permease com... 147 1e-35 >BS_yurM [G] COG0395 ABC-type sugar transport system, permease component Length = 300 Score = 168 bits (426), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 91/245 (37%), Positives = 141/245 (57%), Gaps = 1/245 (0%) Query: 1 MASFKTNAEILNNSLGWPSSFRFSNYVMAFKIAPLSRFYINSVIVGIFGTXXXXXXXGMA 60 M++FK + +I +S PSS+ N+V A+ +S +++NSVIV A Sbjct: 57 MSAFKNSDDIFEHSWSLPSSWHPENFVSAWNQG-ISSYFMNSVIVTALTCVITVFISAWA 115 Query: 61 GYVLARFQFRFKNLLMGAFSLSLLIPGAAMLQPLYLTVNTLGLYDKVIGLIIVYAGFGLP 120 Y L+RF+F+ K + L++ L PLY + +LGLY+ LI+ YA + +P Sbjct: 116 AYGLSRFEFKGKGFFLVLCLGGLMLTPQVSLVPLYSIIQSLGLYNTYWALILPYAAYRIP 175 Query: 121 VSLYILSSYFLTIPKEMEESAYLDGASFIQTFFRIILPISKPGFGTAGVMQFLLCWNEFQ 180 ++ ++ SYFL+I KE+EE+AYLDG + FFRI LP+S P T+G++ WNEF Sbjct: 176 FTIILIRSYFLSISKELEEAAYLDGCTSFGVFFRIFLPMSVPILVTSGILTAYHTWNEFM 235 Query: 181 FAIILTTGNQSRTLPLALYYFKSQFASDYGVMFAATMVVIVPSILVYILLQKQVVSGLAA 240 FAII RT+P L F+ +D+GV+ A + P I++++L+QK V G+A+ Sbjct: 236 FAIIFIDDENLRTIPAGLMQFRDALQTDWGVLLAGLTISAAPIIILFLLMQKYFVRGIAS 295 Query: 241 GAVKG 245 G+VKG Sbjct: 296 GSVKG 300 >BMEI1714 [G] COG0395 ABC-type sugar transport system, permease component Length = 294 Score = 157 bits (396), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 83/251 (33%), Positives = 137/251 (54%), Gaps = 6/251 (2%) Query: 1 MASFKTNAEILNNSLGW-PSSFRFSNYVMAFKIA-----PLSRFYINSVIVGIFGTXXXX 54 ++S + EI+ W P Y F A P+ ++ NS+IV + T Sbjct: 44 LSSLRPPVEIMAKPPVWIPQDISLDAYRTMFSGAGAGGVPVWDYFRNSIIVSVTSTVVSL 103 Query: 55 XXXGMAGYVLARFQFRFKNLLMGAFSLSLLIPGAAMLQPLYLTVNTLGLYDKVIGLIIVY 114 GY ARF+FR K+ F L+ +PG A+ PL++ + +G+ D GLI+ Y Sbjct: 104 IIGMSGGYAFARFRFRGKSATFLGFMLTRSVPGIALSLPLFMVYSRIGIIDTHFGLILTY 163 Query: 115 AGFGLPVSLYILSSYFLTIPKEMEESAYLDGASFIQTFFRIILPISKPGFGTAGVMQFLL 174 +P + +++ +F +PK++ E+A +DG + Q F+++ P++ PG +AG+ FL Sbjct: 164 VALNIPFTTWLIDGFFRQVPKDLAEAAQIDGCTRWQAFWQVEFPLAGPGIASAGIFAFLT 223 Query: 175 CWNEFQFAIILTTGNQSRTLPLALYYFKSQFASDYGVMFAATMVVIVPSILVYILLQKQV 234 CWNE+ A LT S+TLP+ L + ++F D+ M A +V+IVP++ + ++QK + Sbjct: 224 CWNEYALASQLTRSVNSKTLPVGLLDYTAEFTIDWRGMCALAVVMIVPALALTFVIQKHL 283 Query: 235 VSGLAAGAVKG 245 VSGL GAVKG Sbjct: 284 VSGLTFGAVKG 294 >mll0851 [G] COG0395 ABC-type sugar transport system, permease component Length = 282 Score = 151 bits (381), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 76/229 (33%), Positives = 128/229 (55%) Query: 17 WPSSFRFSNYVMAFKIAPLSRFYINSVIVGIFGTXXXXXXXGMAGYVLARFQFRFKNLLM 76 WPS N+V + R+++NSVIV + +AGY ++RF FR K L Sbjct: 54 WPSRMTLQNFVTVLEATDFPRYFLNSVIVSVSTAALVTVTATLAGYAMSRFTFRGKAALA 113 Query: 77 GAFSLSLLIPGAAMLQPLYLTVNTLGLYDKVIGLIIVYAGFGLPVSLYILSSYFLTIPKE 136 L+ P ++ P+Y + +GL + + GLII+Y F + +++ S+F IPK+ Sbjct: 114 LMLLLTQTFPLVMVIPPIYRVMGQIGLINSLTGLIIIYTAFNTAFATFLMQSFFDGIPKD 173 Query: 137 MEESAYLDGASFIQTFFRIILPISKPGFGTAGVMQFLLCWNEFQFAIILTTGNQSRTLPL 196 +EE+A +DG + Q ++I+P++ PG G F W+E FA++L + + +T + Sbjct: 174 LEEAAMIDGCTRAQAMRKVIVPLTLPGMGATLGFVFTAAWSELLFALMLISSDDQKTFAV 233 Query: 197 ALYYFKSQFASDYGVMFAATMVVIVPSILVYILLQKQVVSGLAAGAVKG 245 L F +FA D+G M AA+++ ++P + + LQ+ +V+GL AGAVKG Sbjct: 234 GLLTFIGKFAVDWGQMMAASILALIPVCIFFAFLQRYLVTGLTAGAVKG 282 >BH1246 [G] COG0395 ABC-type sugar transport system, permease component Length = 273 Score = 149 bits (375), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 84/244 (34%), Positives = 144/244 (59%) Query: 1 MASFKTNAEILNNSLGWPSSFRFSNYVMAFKIAPLSRFYINSVIVGIFGTXXXXXXXGMA 60 + S K EI + + + F +YV A PL + +NS ++ T +A Sbjct: 29 LTSIKPPREIFASFAWFSAEPTFQSYVNAVNNRPLFTYMMNSFVISSLTTLIAIACASLA 88 Query: 61 GYVLARFQFRFKNLLMGAFSLSLLIPGAAMLQPLYLTVNTLGLYDKVIGLIIVYAGFGLP 120 Y L R + K L++G + + P A++ P+Y + LGL + GL+I Y LP Sbjct: 89 AYALTRLPIKGKGLILGTVLAASMFPQIAIITPIYSFITDLGLRNSYFGLVIPYITISLP 148 Query: 121 VSLYILSSYFLTIPKEMEESAYLDGASFIQTFFRIILPISKPGFGTAGVMQFLLCWNEFQ 180 ++++IL+++F IP E+EESA LDGA+ QTF +II P++ PG T ++ F+ WNE+ Sbjct: 149 LAIWILATFFKKIPFELEESAKLDGATPFQTFRKIIFPLAAPGVFTTAILVFIAAWNEYL 208 Query: 181 FAIILTTGNQSRTLPLALYYFKSQFASDYGVMFAATMVVIVPSILVYILLQKQVVSGLAA 240 FA+ + T ++ RT+P+ L ++SQ+ +G + AAT++V VP +++ +L Q+++V+GL + Sbjct: 209 FALTINTADRLRTVPVGLSLYESQYTIPWGDITAATVIVTVPIVIIVLLFQRRIVAGLTS 268 Query: 241 GAVK 244 G+VK Sbjct: 269 GSVK 272 >Rv1237 [G] COG0395 ABC-type sugar transport system, permease component Length = 274 Score = 147 bits (372), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 86/245 (35%), Positives = 141/245 (57%), Gaps = 3/245 (1%) Query: 3 SFKTNAEILNNSLGWPSSFRFSNYVMAFKIAPLSRFYINSVIVGIFGTXXXXXXXGMAGY 62 S K + + + L PS+ F NY F+ S INS+ +G+ T MA Y Sbjct: 31 SLKPTSTVKDGKL-IPSTVTFDNYRGIFRGDLFSSALINSIGIGLITTVIAVVLGAMAAY 89 Query: 63 VLARFQFRFKNLLMGAFSLSLLIPGAAMLQPLYLTVNTLGLYDKVIGLIIVYAGFGLPVS 122 +AR +F K LL+GA L + P +++ PL+ +GL+D GLI+ Y F LP++ Sbjct: 90 AVARLEFPGKRLLIGAALLITMFPSISLVTPLFNIERAIGLFDTWPGLILPYITFALPLA 149 Query: 123 LYILSSYFLTIPKEMEESAYLDGASFIQTFFRIILPISKPGFGTAGVMQFLLCWNEFQFA 182 +Y LS++F IP ++E++A +DGA+ Q F ++I+P++ PG TA ++ F+ WN+ A Sbjct: 150 IYTLSAFFREIPWDLEKAAKMDGATPGQAFRKVIVPLAAPGLVTAAILVFIFAWNDLLLA 209 Query: 183 IILTTGNQSRTLPLALYYF--KSQFASDYGVMFAATMVVIVPSILVYILLQKQVVSGLAA 240 + LT + T P+A+ F SQF G + A +V+ +P I+ ++ Q+++V+GL + Sbjct: 210 LSLTATKAAITAPVAIANFTGSSQFEEPTGSIAAGAIVITIPIIVFVLIFQRRIVAGLTS 269 Query: 241 GAVKG 245 GAVKG Sbjct: 270 GAVKG 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,046,738 Number of extensions: 616216 Number of successful extensions: 6855 Number of sequences better than 1.0e-05: 196 Number of HSP's gapped: 6719 Number of HSP's successfully gapped: 199 Length of query: 245 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 141 Effective length of database: 26,383,695 Effective search space: 3720100995 Effective search space used: 3720100995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 114 (48.5 bits)