BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0012.p (1475 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2538 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hy... 115 7e-25 lin2540 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hy... 79 4e-14 >lin2538 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hydrolases Length = 1310 Score = 115 bits (287), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 82/233 (35%), Positives = 121/233 (51%), Gaps = 9/233 (3%) Query: 655 YVTGLSRANSSVEFSVNVENAASYDVKLRYAKEAA-GKTLGLYLNGTKIKNITLPPTGGP 713 YV G S NV++A Y+V L Y + ++L +++NG +K TL P Sbjct: 912 YVDGYDSDGSGTTIYANVKDAGDYNVDLTYKNASEDNQSLSIFVNGEYVKQTTLKPVAD- 970 Query: 714 TVWKEQLETLSLKEGTNTITYKNGTD---NSGIINIDSIHL-TKRTPWRYQAEEAARQGS 769 W Q E L L G N+I+YK TD + + +D +++ T + +AEEAA G+ Sbjct: 971 --WTVQAENLPLAAGKNSISYKVVTDTGDKADQVALDKVNIGFTPTVAKVEAEEAALSGT 1028 Query: 770 LQIAKDHLWYEGNGFVGGFEQKGDSLKFEVNVPNTAAYTSALRYSGAQSSNITMTMYVNG 829 L++A DH +Y G GFVGGF GD +KFEV+VP Y R + T+ +Y NG Sbjct: 1029 LKVANDHWFYSGEGFVGGFGAAGDEIKFEVDVPADGEYVLNTRTANGSGMPQTLDLYTNG 1088 Query: 830 QRIKQVSLPPTA-SWDSWANRTESVNLNAGKNVIEFIREQGDTGRFNIDSLTI 881 +V+ P +W+ W + ++VNL AG N I F+ + TG NID + I Sbjct: 1089 TYNSRVTFPSEGENWNIWTDTEKAVNLKAGTNKITFLMDGETTGNINIDRILI 1141 Score = 83.2 bits (204), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 74/220 (33%), Positives = 106/220 (48%), Gaps = 16/220 (7%) Query: 413 NTKAAGTYSLDFRYNNNTANAINMKLGVNQEAVRDISFASNAGFEANFDIQSAYNIKLNA 472 N K AG Y++D Y N + + ++ + VN E V+ + A++ +Q A N+ L A Sbjct: 928 NVKDAGDYNVDLTYKNASEDNQSLSIFVNGEYVKQTTLKP----VADWTVQ-AENLPLAA 982 Query: 473 GSNKIRL-------SGKSSLALDAMIIKKSTLY---EAENAELSGNAKVDTDHPGYSGTG 522 G N I +ALD + I + EAE A LSG KV DH YSG G Sbjct: 983 GKNSISYKVVTDTGDKADQVALDKVNIGFTPTVAKVEAEEAALSGTLKVANDHWFYSGEG 1042 Query: 523 FAGGLWISNSAVTFKVNAANAGSYSVNLGYSLGFNDDRTLSMYVNGQKIKQVDFFSL-KS 581 F GG + + F+V+ G Y +N + G +TL +Y NG +V F S ++ Sbjct: 1043 FVGGFGAAGDEIKFEVDVPADGEYVLNTRTANGSGMPQTLDLYTNGTYNSRVTFPSEGEN 1102 Query: 582 WNSWADRYDNVFLKQGENTIMYKYDSGDTGNVNLDYITVT 621 WN W D V LK G N I + D TGN+N+D I ++ Sbjct: 1103 WNIWTDTEKAVNLKAGTNKITFLMDGETTGNINIDRILIS 1142 Score = 81.6 bits (200), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 104/363 (28%), Positives = 167/363 (46%), Gaps = 39/363 (10%) Query: 410 FTVNTKAAGTYSLD-----FRYNNNTANAINMKLGVNQEAVRDISFASNA---GFEANFD 461 FTV K G+Y D + + N A ++ + G + + D+S NA G+ + D Sbjct: 799 FTVGAKE-GSYKADTENYIVKLHGNAAESVQVN-GTDSKLYDDLSALENADGSGYAVSSD 856 Query: 462 IQSAYN-IKLNAGSNK---IRLSGKSSLALDAMIIKKSTLYEAENAELSGNAKVDTDHPG 517 I A +K+ A + I +G S ++++ YE E S A + P Sbjct: 857 IYGAVTYVKVPAQGDADTVITATGSKS-------VEQTGTYEMEKG--SSFANTVAEKPV 907 Query: 518 YSGTGFAGGLWISNSAVTFKVNAANAGSYSVNLGYSLGFNDDRTLSMYVNGQKIKQVDFF 577 T + G S T N +AG Y+V+L Y D+++LS++VNG+ +KQ Sbjct: 908 TEQT-YVDGYDSDGSGTTIYANVKDAGDYNVDLTYKNASEDNQSLSIFVNGEYVKQT--- 963 Query: 578 SLKSWNSWADRYDNVFLKQGENTIMYKY--DSGDTGN-VNLDYITV-TEATTWHYGAEDA 633 +LK W + +N+ L G+N+I YK D+GD + V LD + + T AE+A Sbjct: 964 TLKPVADWTVQAENLPLAAGKNSISYKVVTDTGDKADQVALDKVNIGFTPTVAKVEAEEA 1023 Query: 634 TITDPDDAEVVYAKDGNLGTG--YVTGLSRANSSVEFSVNVENAASYDVKLRYAK-EAAG 690 ++ + A D +G +V G A ++F V+V Y + R A Sbjct: 1024 ALS----GTLKVANDHWFYSGEGFVGGFGAAGDEIKFEVDVPADGEYVLNTRTANGSGMP 1079 Query: 691 KTLGLYLNGTKIKNITLPPTGGP-TVWKEQLETLSLKEGTNTITYKNGTDNSGIINIDSI 749 +TL LY NGT +T P G +W + + ++LK GTN IT+ + +G INID I Sbjct: 1080 QTLDLYTNGTYNSRVTFPSEGENWNIWTDTEKAVNLKAGTNKITFLMDGETTGNINIDRI 1139 Query: 750 HLT 752 ++ Sbjct: 1140 LIS 1142 >lin2540 [G] COG1501 Alpha-glucosidases, family 31 of glycosyl hydrolases Length = 1090 Score = 79.3 bits (194), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 47/124 (37%), Positives = 72/124 (58%), Gaps = 4/124 (3%) Query: 759 YQAEEAARQGSLQIAKDHLWYEGNGFVGGFEQKGDSLKFEVNVPNTAA-YTSALRYSGAQ 817 Y+AE ++ A DH Y G GFV GF+++ ++++F+++ + A+ YT +RYS A Sbjct: 969 YEAE-FGHLTNVSTASDHAGYTGTGFVAGFDEEKEAVEFDIDAVDGASDYTMEVRYS-AG 1026 Query: 818 SSNITMTMYVNGQRIKQVSLPPTASWDSWANRTESVNLNAGKNVIEFIREQGDTGRFNID 877 + T T+YVNG + +Q++LP TA WD+W V L G N + F E GDT N D Sbjct: 1027 VEDATRTVYVNGNK-QQIALPKTADWDTWDVVEVPVTLQDGINQVVFDFEAGDTAGINFD 1085 Query: 878 SLTI 881 + I Sbjct: 1086 HVVI 1089 Score = 74.3 bits (181), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 43/124 (34%), Positives = 70/124 (56%), Gaps = 4/124 (3%) Query: 498 YEAENAELSGNAKVDTDHPGYSGTGFAGGLWISNSAVTFKVNAANAGS-YSVNLGYSLGF 556 YEAE L+ N +DH GY+GTGF G AV F ++A + S Y++ + YS G Sbjct: 969 YEAEFGHLT-NVSTASDHAGYTGTGFVAGFDEEKEAVEFDIDAVDGASDYTMEVRYSAGV 1027 Query: 557 NDDRTLSMYVNGQKIKQVDFFSLKSWNSWADRYDNVFLKQGENTIMYKYDSGDTGNVNLD 616 +D T ++YVNG K +Q+ W++W V L+ G N +++ +++GDT +N D Sbjct: 1028 -EDATRTVYVNGNK-QQIALPKTADWDTWDVVEVPVTLQDGINQVVFDFEAGDTAGINFD 1085 Query: 617 YITV 620 ++ + Sbjct: 1086 HVVI 1089 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.312 0.130 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 109,562,417 Number of extensions: 4731287 Number of successful extensions: 7759 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 7745 Number of HSP's successfully gapped: 6 Length of query: 1475 Length of database: 38,378,431 Length adjustment: 119 Effective length of query: 1356 Effective length of database: 24,653,685 Effective search space: 33430396860 Effective search space used: 33430396860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 122 (51.6 bits)