BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0010.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2253 [T] COG4585 Signal transduction histidine kinase 139 4e-33 all4636 [T] COG4585 Signal transduction histidine kinase 108 6e-24 CAC2940 [T] COG4585 Signal transduction histidine kinase 108 9e-24 CAC1454 [T] COG4585 Signal transduction histidine kinase 107 2e-23 CAC0864 [T] COG4585 Signal transduction histidine kinase 99 5e-21 >CAC2253 [T] COG4585 Signal transduction histidine kinase Length = 378 Score = 139 bits (351), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 84/236 (35%), Positives = 132/236 (55%), Gaps = 14/236 (5%) Query: 36 FRNVLIEKARTQELNQELQIANTTLQEYSNKIQELTISQERTRIAQELHDSLGHYLIALN 95 F + I K + + +L+I N+ L EYS +E +S+ER RI+QELHDS+GH L+AL+ Sbjct: 149 FVFIFIHKFKDER--DKLKILNSNLIEYSFNEREFLLSEERNRISQELHDSIGHSLMALS 206 Query: 96 MNLEYASTI-----VDVETDKAKLAIQKSQQLSKECIVNLRKTVVLLGDERASKELLQSL 150 MN++Y I ++ E D I++S Q + + NL+K DE +L + + Sbjct: 207 MNIKYLKGIKNRSNINEELDNMDKVIKESVQTLRSTVYNLKKL-----DECC--DLKKEV 259 Query: 151 HEIFDNFLETNKIQFELNMDEDIEYVDSDIKNCIYKTVQESITNGIKHGQATHFSIAIHK 210 ++I F + ++ + DIE + IKN + KT++E ITN +KHG T ++ + Sbjct: 260 YDIIKKFNRLDIVKINFDCASDIEKSTTTIKNTLIKTIKEGITNSLKHGNPTEINVTLTL 319 Query: 211 YSDHISLLVHNNGADCKEIIKSKGIQGIENRILALGGKVVFYSGESSGFRVEANIP 266 + +I L++ +NG C+ I S G+ GI+ RI AL GKV F S GF +E IP Sbjct: 320 VNSYIKLIIQDNGVGCQNIKPSAGLNGIKKRIEALNGKVAFESKARKGFTIETEIP 375 >all4636 [T] COG4585 Signal transduction histidine kinase Length = 401 Score = 108 bits (271), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 67/219 (30%), Positives = 121/219 (55%), Gaps = 4/219 (1%) Query: 51 QELQIANTTLQEYSNKIQELTISQERTRIAQELHDSLGHYLIALNMNLEYASTIVDVETD 110 +EL +AN L++Y+ +I+ +ER RIA+E+HDSLGH L ALN+ LE A + + Sbjct: 179 EELAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPN 238 Query: 111 KAKLAIQKSQQLSKECIVNLRKTV-VLLGDERASKELLQSLHEIFDNFLETNKIQFELNM 169 KA+ + ++++L + + ++R +V + K L Q++ I +F + I + Sbjct: 239 KAQTFLVRAKELGSKALQDVRNSVSAMRSHPLQGKTLEQAIDIIAADFEQNTGIILTSSF 298 Query: 170 DEDIEYVDSDIKNCIYKTVQESITNGIKHGQATHFSIAIHKYSDHISLLVHNNGA--DCK 227 + D + + +++ IY+ +QES+TN KH +AT + + +SL++ +NG D + Sbjct: 299 NLDYK-LSTEVSTAIYRIIQESLTNISKHARATEVILEVSLNRGSLSLIIRDNGRGFDIQ 357 Query: 228 EIIKSKGIQGIENRILALGGKVVFYSGESSGFRVEANIP 266 + G+Q + +R LALGGK S G ++ +IP Sbjct: 358 QNTTGFGLQSMRDRTLALGGKFAINSAVGDGCQITVDIP 396 >CAC2940 [T] COG4585 Signal transduction histidine kinase Length = 402 Score = 108 bits (270), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 64/224 (28%), Positives = 120/224 (53%), Gaps = 1/224 (0%) Query: 45 RTQELNQELQIANTTLQEYSNKIQELTISQERTRIAQELHDSLGHYLIALNMNLEYASTI 104 R +E ++L+ A L+ YS ++EL+I +ER RI++E+HDS+GH L A+ M L+ + Sbjct: 168 RLRESEEKLRGAYEKLERYSETVEELSILRERNRISREIHDSVGHTLSAVLMQLQALLYL 227 Query: 105 VDVETDKAKLAIQKSQQLSKECIVNLRKTVVLLGDERASK-ELLQSLHEIFDNFLETNKI 163 + + +++ +++ + I N+R+TV L + E + ++ E+ + + Sbjct: 228 IKSDKEESLKTVEEMTGFVRGGIENVRRTVRELKPTDFDRFEGVFAIEEMIRKYKKLTSA 287 Query: 164 QFELNMDEDIEYVDSDIKNCIYKTVQESITNGIKHGQATHFSIAIHKYSDHISLLVHNNG 223 + ++ + SD Y+ VQE++ N I+HG+A+ +++ D + + + +NG Sbjct: 288 DIRFILSKEKRSLSSDESFISYRVVQEALNNAIRHGKASLVEVSMQFNQDRLYIRIKDNG 347 Query: 224 ADCKEIIKSKGIQGIENRILALGGKVVFYSGESSGFRVEANIPK 267 I K G+ G+E R+ LGG + FYS GF V N+PK Sbjct: 348 VGTDGIKKGFGMTGMEERVKKLGGNIEFYSERDKGFEVTVNLPK 391 >CAC1454 [T] COG4585 Signal transduction histidine kinase Length = 441 Score = 107 bits (267), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 65/218 (29%), Positives = 119/218 (54%), Gaps = 3/218 (1%) Query: 50 NQELQIANTTLQEYSNKIQELTISQERTRIAQELHDSLGHYLIALNMNLEYASTIVDVET 109 N++L++ N L+EY+ KI + ++ER R+A+E+HD+LGH L +++ ++ T +DV Sbjct: 219 NEKLEVMNVQLKEYAVKIGSMAETRERNRMAREIHDTLGHALTGISVGIDACLTTIDVSK 278 Query: 110 DKAKLAIQKSQQLSKECIVNLRKTV-VLLGDERASKELLQSLHEIFDNFLETNKIQFELN 168 + K + +++E I ++R++V L D + L +L+ + + K + LN Sbjct: 279 ELTKKQLNIIADVAREAIKDVRRSVKKLRPDALENSSLQDALNNMISRISKATKTKVFLN 338 Query: 169 MDEDIEYVDSDIKNCIYKTVQESITNGIKHGQATHFSIAIHKYSDHISLLVHNNGADCKE 228 + + D ++ IY+ +QE ITN I+HGQA I + + +++ + +NG C Sbjct: 339 ANIKLLKFGHDEEDTIYRIIQEGITNSIRHGQAEEIYIDMIRRDKQLNITIRDNGKGCAN 398 Query: 229 IIKSKGIQGIENRILALGGKVVFYSGESSGFRVEANIP 266 + + G++ IE R+ L G V Y G S F + NIP Sbjct: 399 VEEGFGLRHIEERVKLLNGTVE-YDG-SDSFLITVNIP 434 >CAC0864 [T] COG4585 Signal transduction histidine kinase Length = 392 Score = 99.4 bits (246), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 61/229 (26%), Positives = 123/229 (53%), Gaps = 3/229 (1%) Query: 40 LIEKARTQELNQELQIANTTLQEYSNKIQELTISQERTRIAQELHDSLGHYLIALNMNLE 99 L ++ R E ++L+ AN L+ Y++ ++ELT+ +ER RI++E+HDS+GH L + + L Sbjct: 152 LYDRLRISE--EKLKKANRDLEMYASSVEELTLLRERNRISREIHDSVGHSLSTMVIQLG 209 Query: 100 YASTIVDVETDKAKLAIQKSQQLSKECIVNLRKTV-VLLGDERASKELLQSLHEIFDNFL 158 I+ +KA+L + ++ +++ + +R V + E E + S+ E+ NF Sbjct: 210 AIEKIIRKAPEKAELLTKNLRKFTQKSLDEVRAAVRAIKPKEFEEYEGILSIEELISNFK 269 Query: 159 ETNKIQFELNMDEDIEYVDSDIKNCIYKTVQESITNGIKHGQATHFSIAIHKYSDHISLL 218 + I L ++ ++SD +Y+ VQE ++N +HG+A+ + + ++ + Sbjct: 270 KMANIDVRLAFTKEKWSLNSDQSFVLYRIVQEFLSNSARHGRASRVDVIMAFSEKNLLVT 329 Query: 219 VHNNGADCKEIIKSKGIQGIENRILALGGKVVFYSGESSGFRVEANIPK 267 + +NG C+ II+ G++ + R+ LGG + + GF V+ + K Sbjct: 330 LKDNGQGCQSIIEGVGMKSMRERVKELGGYFEYKTAPYEGFTVKIELAK 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,214,265 Number of extensions: 686021 Number of successful extensions: 2484 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 2445 Number of HSP's successfully gapped: 52 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)