BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0006.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0170 [V] COG0842 ABC-type multidrug transport system, permeas... 267 2e-71 BS_yfiM [V] COG0842 ABC-type multidrug transport system, permeas... 77 5e-14 CAC0242 [V] COG0842 ABC-type multidrug transport system, permeas... 75 1e-13 BH1055 [V] COG0842 ABC-type multidrug transport system, permease... 70 6e-12 CAC0171 [V] COG0842 ABC-type multidrug transport system, permeas... 68 3e-11 >CAC0170 [V] COG0842 ABC-type multidrug transport system, permease component Length = 420 Score = 267 bits (683), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 140/336 (41%), Positives = 204/336 (60%), Gaps = 6/336 (1%) Query: 47 IKTFNVAFYNADKGSSEGTXXXXXSLGNVLRDNVFKQEDLKQIVNLKETTSYKQGKQLLQ 106 +K FNVA+Y+AD G ++ L DNVF+ +D+K+I+NLK+ SY + K+L+ Sbjct: 47 LKVFNVAYYSADTGYQS------LNVSKNLSDNVFENKDIKKIINLKKVRSYAEAKELVS 100 Query: 107 DGKAAVFVYVPETFTKTAMNNEKTDIVLMGDNDKPLDKEIVATILDRFNASVRTMNVEQR 166 +G A+ VYVP FTK +N +I ++GDN+K D IV TI++ FN ++ T+ EQ Sbjct: 101 NGTASALVYVPTNFTKDYLNGNNVEISVVGDNNKINDVSIVKTIVNSFNENISTIRAEQN 160 Query: 167 TILQSISSTGNIPKDKIEKVMSQISGNKQLSLKIPVVPTNKDAVPIDAMQYYSIAMVVMF 226 + + + + + I+K++ I +S KI V + P++ MQY AMVVMF Sbjct: 161 EVEAEVKNKAFLKQVDIDKLIKSIENTNGISAKISKVASKSGVKPLNIMQYEIFAMVVMF 220 Query: 227 SIMTAFVLVHSIVDEKQNHTLFRIKSTPTLNIQYVLGKLFGXXXXXXXXXXXXXXXXXXX 286 SI+TAF L +IV +K N+T+ RIKST T N QY+L K+ G Sbjct: 221 SIITAFELTQNIVSDKLNNTMNRIKSTSTSNFQYILSKIIGMVIAIIVQMVTVMVISSVV 280 Query: 287 FQMKWGNPLELLVITIVYAFAIGSIILLWGFTAKDHVTVSSLASPVLYIFSFLGGSFITK 346 F++K+ N +++IT+VY FAIGSI++ G AKDH+ VSS+ SP+++ FSFLGGS K Sbjct: 281 FRVKYDNIPGIILITVVYGFAIGSIVVCAGLFAKDHMAVSSVMSPIVWGFSFLGGSLFDK 340 Query: 347 SGLPDSLRVIQEIIPNGKAINSYLKVCQGVGLSGMY 382 + PD L IQ IIPNGKAIN YL+VC+G + +Y Sbjct: 341 NNFPDVLACIQRIIPNGKAINCYLEVCEGNSIGFIY 376 >BS_yfiM [V] COG0842 ABC-type multidrug transport system, permease component Length = 90 Score = 77.0 bits (188), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 0/349 (0%), Positives = 0/349 (0%), Gaps = 31/349 (8%) Query: 42 DSSSKIKTFNVAFYNADKGSSEGTXXXXXSLGNVLRDNVFKQEDLKQIVNLKETT--SYK 99 S + + + +GS + + T + Sbjct: 45 MFSVSLGASKELWDSRPEGSGW---------------SWKDLAWDVAGASTGYTVWQLTR 89 Query: 100 QGKQLLQDGKAAVFVYVPETFTKTAMNNEKTDIVLMGDNDKPLDKEIVATILDRFNASVR 159 Sbjct: 90 ----- 144 Query: 160 TMNVEQRTILQSISSTGNIPKDKI------EKVMSQISGNKQLSLKIPVVPTNKDAVPID 213 Sbjct: 145 -- 202 Query: 214 AMQYYSIAMVVMFSIMTAFVLVHSIVDEKQNHTLFRIKSTPTLNIQYVLGKLFGXXXXXX 273 Sbjct: 203 262 Query: 274 XXXXXXXXXXXXXFQMKWG-NPLELLVITIVYAFAIGSIILLWGFTAKDHVTVSSLASPV 332 Sbjct: 263 322 Query: 333 LYIFSFLGGSFITKSGLPDSLRVIQEIIPNGKAINSYLKVCQGVGLSGM 381 Sbjct: 323 371 >CAC0242 [V] COG0842 ABC-type multidrug transport system, permease component Length = 378 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 75/296 (25%), Positives = 125/296 (42%), Gaps = 38/296 (12%) Query: 78 DNVFKQEDLKQIVNLKETTSYKQGKQLLQDGKAAVFVYVPETFTKTAMNNEKTDIVLMGD 137 D K + +K I+N+K+ +SYK+GK+L+ G+ +Y+ ++ N+K I + Sbjct: 70 DEFLKNKSIKDILNVKKVSSYKKGKELVDKGEGNSLIYINSNYSDNIEANKKGRIEIYES 129 Query: 138 NDKPLDKEIVATILDRFNASVRTMNVEQRTILQSISSTGNIPKDKIEKVMSQISGNKQLS 197 +I+ +++D FN T+ V + S T + K+ I K ++G Sbjct: 130 KSSTTRNQIIKSLIDSFNDGANTVEVSSQI---SRMRTSYVEKNNISKKYLSVNG----- 181 Query: 198 LKIPVVPTNKDAVPIDAMQYYSIAMVVMFSIMTAFVLVHSIVDEKQNHTLF------RIK 251 K P A+ YYS+ M++M +L + + LF RIK Sbjct: 182 -KSP-----------RAIDYYSVTMLMM------ILLYGANYGSAELENLFFDRVGKRIK 223 Query: 252 STPTLNIQYVLGKLFGXXXXXXXXXXXXXXXXXXXFQMKWGNPLELLVITIVYAFAI--G 309 +T T Q++LG + G + WG +LV+ IV +FAI Sbjct: 224 TTATKTYQHLLGVVLGVIFTLILQAVVLVLFTKYVYGANWGGH-PILVLGIVSSFAILAA 282 Query: 310 SIILLWGFTAKDHVTVSSLASPVLYIFSFLGGSFITKSGLPDSLRVIQEIIPNGKA 365 SI L+ D S L S IF+F+ G ++ +P S I + +PN A Sbjct: 283 SIGFLFMIITGDDKRASMLISVTAPIFTFVSGGYVK---VPFSDGSIIKYVPNNLA 335 >BH1055 [V] COG0842 ABC-type multidrug transport system, permease component Length = 423 Score = 70.5 bits (171), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 76/321 (23%), Positives = 140/321 (43%), Gaps = 30/321 (9%) Query: 71 SLGNVLRDNVFKQEDLKQIVNLKETTSYKQGKQLLQDGKAAVFVYVPE-----TFTKTAM 125 SL ++L D + E L + L + + ++ DG +Y PE T+ + + Sbjct: 94 SLPDLLIDQILDNEQLS-FIELTHVDALDEVQRADYDGV----IYFPEGYRIATWKQVFL 148 Query: 126 NNEKTDIVLMGDNDK-PLDKEIVATILDRFNASVRTMNVEQRTILQSISSTGNIPKDKIE 184 ++ + N+K PL IV I+D+ R N+ Q ++ + S+ IP + Sbjct: 149 GEDQVPAAQVYLNEKAPLRASIVDQIVDQLY---RYWNI-QMSLNEIASAQQRIPLEPNW 204 Query: 185 KVMSQISGNKQLSLKIPVVPTNKDAVPIDAMQYYSIAMVVMFSI-MTAFVLVHSIVDEKQ 243 V + + + PI + YY++ M VMF++ + AF+ + + EKQ Sbjct: 205 FVPDSVGSWEFF----------DEREPISSFDYYTVGMCVMFALFVVAFIASYGQL-EKQ 253 Query: 244 NHTLFRIKSTPTLNIQYVLGKLFGXXXXXXXXXXXXXXXXXXXFQMKWGNPLELLVITIV 303 R+ Y++ K +Q + L +VITI+ Sbjct: 254 QQVFQRVMLANVSPWVYMISKWVSGTLISFVQLIIVFTLSSLVYQTTFPTVLPFVVITIM 313 Query: 304 YAFAIGSI-ILLWGFTAKDHVT--VSSLASPVLYIFSFLGGSFITKSGLPDSLRVIQEII 360 ++ +G++ +L+ F + T ++ +S ++ I +F+GGSF L +SL + + Sbjct: 314 FSITVGALAVLMTSFNYRFQTTSLITVFSSGIVSILAFVGGSFFDIGELSESLYRLGQWT 373 Query: 361 PNGKAINSYLKVCQGVGLSGM 381 PNG A+NSYL + G LSG+ Sbjct: 374 PNGAALNSYLYLLSGGELSGL 394 >CAC0171 [V] COG0842 ABC-type multidrug transport system, permease component Length = 391 Score = 68.2 bits (165), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 77/323 (23%), Positives = 154/323 (47%), Gaps = 24/323 (7%) Query: 55 YNADKGSSEGTXXXXXSLGNVLRDNVFKQEDLKQIVNLKETTSYKQGKQLLQDGKAAVFV 114 Y AD+ ++E + L N+L++N DL I ++S + K + D V V Sbjct: 48 YFADQDNTEASR----QLVNMLKNN----HDLNLI-----SSSESEIKNKVDDDNITVGV 94 Query: 115 YVPETFTKTAMNNEKTDIVLMGDNDKPLDKEIVATILDRFNASVRTMNVEQRTILQSISS 174 + + F NN+ DI L+ D D +D E ++ I+ +++ +N++ ++I + +SS Sbjct: 95 LINKGFGYKLQNNKALDITLIEDYD-SVDGEKLSDIILSTENTLQKVNLDSKSISRVLSS 153 Query: 175 TGNIPKDKIEKVMSQISGNKQLSLKIPVVPTNKDAVPIDAMQYYSIAMVVMFSIMTAFVL 234 + +KI K ++ SG K L + +D + + + + M + F ++ F Sbjct: 154 NESSISNKIIKKINNGSGVKLLYKNLKSSQNVEDKNAQNLIGF--LLMFLWFIVIQGF-- 209 Query: 235 VHSIVDEKQNHTLFRIKSTPTLNIQYVLGKLFGXXXXXXXXXXXXXXXXXXXFQMKWGNP 294 ++++EK+N+T RIK TPT +Y+L K+ + K N Sbjct: 210 -RTLIEEKENNTFNRIKGTPTNYSKYLLSKIIANYIFGMIIIAIILIVGKYGLKSKIINN 268 Query: 295 L--ELLVITIVYAFAIGSIILLWGFTAKDHVTVSSLASPVLYIFSFLGGSFIT--KSGLP 350 + E+ +++I Y F+I +++++ K H T + + + ++ + LGGSF + + LP Sbjct: 269 VGPEIAILSI-YLFSIVGLVMIFVPFVKKHQTFTIIGAAIMVLTGLLGGSFFSMDELQLP 327 Query: 351 DSLRVIQEIIPNGKAINSYLKVC 373 ++ +I + +P I S +V Sbjct: 328 KAIEIISKFMPETWGIKSLKEVV 350 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,503,143 Number of extensions: 818502 Number of successful extensions: 1657 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1653 Number of HSP's successfully gapped: 6 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)