BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0003.p (231 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr2045 [Q] COG3208 Predicted thioesterase involved in non-ribos... 153 2e-37 PA2411 [Q] COG3208 Predicted thioesterase involved in non-riboso... 145 6e-35 PA2425 [Q] COG3208 Predicted thioesterase involved in non-riboso... 124 9e-29 PA4229 [Q] COG3208 Predicted thioesterase involved in non-riboso... 112 6e-25 YPO0773 [Q] COG3208 Predicted thioesterase involved in non-ribos... 98 1e-20 >alr2045 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 359 Score = 153 bits (387), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 14/220 (6%), Positives = 42/220 (19%), Gaps = 4/220 (1%) Query: 4 LFCLPYAGGSGSIYYKWSTLLSDTIQLCPIELKGRGKRIHEVFYEDIDEAVNDIYVQLKD 63 A + Y + T+ R + + I + V + Sbjct: 25 PASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDA 84 Query: 64 KIDQHEYAIFGHSMGSRLAYKLYYKIDKMNNRKPKHIFFSGCGAPNIPRRKSMLHSLPDP 123 + + +L + + G + R + Sbjct: 85 RDLP-TISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHR 143 Query: 124 LFIQRIMEMGGTPEEFVTNKDLQKLCLPILRNDFRILEKDRYTEHEQKLGCDISILSGNK 183 L + + D K + + ++ + IL + Sbjct: 144 LTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPG-QRSIAASGGILLWPQ 202 Query: 184 DT-LSAEAIVSWKNYTDRQCKLYMFEGNHFYINHHADDIT 222 + + + + + + Sbjct: 203 SHFDWVRPGIILYGVSPLEDRSTGADFGCQPV-MSLTSSL 241 >PA2411 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 145 bits (365), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 74/227 (32%), Positives = 117/227 (51%), Gaps = 7/227 (3%) Query: 4 LFCLPYAGGSGSIYYKWSTLLSDTIQLCPIELKGRGKRIHEVFYEDIDEAVNDIYVQLKD 63 LFCLPY+G S Y +W L + + P+EL GRG R+ E D+ + +L D Sbjct: 8 LFCLPYSGASAMTYSRWRRKLPAWLAVRPVELPGRGARMAEPLQTDLASLAQQLARELHD 67 Query: 64 KIDQHEYAIFGHSMGSRLAYKLYYKIDKMNNRKPKHIFFSGCGAPNIPRRKSMLHSLPDP 123 ++ Q YA+ GHS+G+ LA ++ Y + ++ P F G AP+ RR +P Sbjct: 68 EVRQGPYAMLGHSLGALLACEVLYALRELGCPTPLGFFACGTAAPS--RRAEYDRGFAEP 125 Query: 124 L----FIQRIMEMGGTPEEFVTNKDLQKLCLPILRNDFRILEKDRYTEHEQKLGCDISIL 179 I + ++ GTPEE + N++L L LPILR DF + R+ + L C I L Sbjct: 126 KSDAELIADLRDLQGTPEEVLGNRELMSLTLPILRADFLLCGSYRH-QRRPPLACPIRTL 184 Query: 180 SGNKDTLSAEAIVSWKNYTDRQCKLYMFEGNHFYINHHADDITAIIN 226 G +D S E +++W T +L +F+G HF+I+ ++ A++ Sbjct: 185 GGREDKASEEQLLAWAEETRSGFELELFDGGHFFIHQREAEVLAVVE 231 >PA2425 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 124 bits (312), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 63/225 (28%), Positives = 107/225 (47%), Gaps = 2/225 (0%) Query: 4 LFCLPYAGGSGSIYYKWSTLLSDTIQLCPIELKGRGKRIHEVFYEDIDEAVNDIYVQLKD 63 LFCLPY+G S ++ +W L I+L P+EL GRG R E + D V + +L+ Sbjct: 9 LFCLPYSGASARLFRRWQPSLPGWIRLLPLELPGRGLRFGEALHTDAASLVAQLAGELQG 68 Query: 64 KIDQHE-YAIFGHSMGSRLAYKLYYKIDKMNNRKPKHIFFSGCGAPNIPRRKSMLHSLPD 122 +D YA FGHS+G LA++L + + + P +F SG P + D Sbjct: 69 TLDSASPYAFFGHSLGGLLAFELAHAMRRRGLEGPLALFLSGVSPPAENDVSGYRRAKAD 128 Query: 123 PLFIQRIMEMGGTPEEFVTNKDLQKLCLPILRNDFRILEKDRYTEHEQKLGCDISILSGN 182 + + + GTPE + + L ++ LP++R DF + RY H L + + G Sbjct: 129 AELVAALRDYRGTPEAVLNDPSLMQMLLPVVRADFLVTGSYRYQAH-GPLDAALHLFGGR 187 Query: 183 KDTLSAEAIVSWKNYTDRQCKLYMFEGNHFYINHHADDITAIINQ 227 +D+L + ++ W + L + +G HF+I + ++ + Sbjct: 188 EDSLRSAELLGWLHEAGGDFTLDLLDGQHFFIREQQGQLLRLLRR 232 >PA4229 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 251 Score = 112 bits (279), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 66/219 (30%), Positives = 109/219 (49%), Gaps = 6/219 (2%) Query: 4 LFCLPYAGGSGSIYYKWSTLLSDTIQLCPIELKGRGKRIHEVFYEDIDEAVNDIYVQLKD 63 L C P+AGGS S + WS L I L ++ GR R +E +++ + + L+D Sbjct: 19 LACFPHAGGSASFFRSWSERLPPDIDLLALQYPGREDRFNEAPATRLEDLADGAALALRD 78 Query: 64 KIDQHEYAIFGHSMGSRLAYKLYYKIDKMNNRKPKHIFFSGCGAPNIPRRKSMLHSLPDP 123 D A+FGHS+G+ LAY+ +++ +H+F S AP+ +R LH + Sbjct: 79 FADA-PLALFGHSLGAALAYETALRLESAGA-PLRHLFVSAHPAPHR-QRGGALHRGDEA 135 Query: 124 LFIQRIMEMGGTPEEFVTNKDLQKLCLPILRNDFRILEKDRYTEHEQKLGCDISILSGNK 183 ++ + GG E + + DL+ L LPILR D++ +E R + L C + +L G Sbjct: 136 ALLEDVRRQGGA-SELLEDADLRALFLPILRADYQAIETYRRAQ-PIALACALDVLLGEH 193 Query: 184 D-TLSAEAIVSWKNYTDRQCKLYMFEGNHFYINHHADDI 221 D +SA +W + + +L F G HFY++ D + Sbjct: 194 DEEVSAAEAQAWSDASRTPARLRRFPGGHFYLSEGRDAV 232 >YPO0773 [Q] COG3208 Predicted thioesterase involved in non-ribosomal peptide biosynthesis Length = 254 Score = 97.8 bits (242), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 64/227 (28%), Positives = 110/227 (48%), Gaps = 6/227 (2%) Query: 4 LFCLPYAGGSGSIYYKWSTLLSDTIQLCPIELKGRGKRIHEVFYEDIDEAVNDIYVQLKD 63 + C +AGGS + W T L L I+L G G RI+E ++ + + D+ Q Sbjct: 17 MVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQ-MADVIAQSYV 75 Query: 64 KIDQHEYAIFGHSMGSRLAYKLYYKIDKMNNRKPKHIFFSGCGAPNIPRRKSMLHSLPDP 123 + + FGHSMG+ LA+++ ++ K R ++ SG P+ + + D Sbjct: 76 YLPVRDTFFFGHSMGAALAFEVALRLQKAG-RGITTLYVSGRLPPH-KNTNTDFYRQSDV 133 Query: 124 LFIQRIMEMGGTPEEFVTNKDLQKLCLPILRNDFRILEKDRYTEHEQKLGCDISILSGNK 183 + +I ++GG+ ++ +L+ L LPI R DF +E + + + L C + G + Sbjct: 134 SILNKITQLGGSTLDY-QQPELKDLLLPIFRADFTAIETYQ-RQCTELLNCSVMACVGEQ 191 Query: 184 DT-LSAEAIVSWKNYTDRQCKLYMFEGNHFYINHHADDITAIINQTL 229 DT +S + W T +L +F G HFY+NH + A INQ++ Sbjct: 192 DTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSV 238 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.140 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,953,724 Number of extensions: 779130 Number of successful extensions: 1837 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 1813 Number of HSP's successfully gapped: 11 Length of query: 231 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 128 Effective length of database: 26,499,029 Effective search space: 3391875712 Effective search space used: 3391875712 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)