BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0057.p (434 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1117 [G] COG1653 ABC-type sugar transport system, periplasmic ... 221 2e-57 BS_yesO [G] COG1653 ABC-type sugar transport system, periplasmic... 204 3e-52 YPO1719 [G] COG1653 ABC-type sugar transport system, periplasmic... 92 2e-18 BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic... 76 1e-13 Cgl1352 [G] COG1653 ABC-type sugar transport system, periplasmic... 65 2e-10 >BH1117 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 434 Score = 221 bits (562), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 141/428 (32%), Positives = 233/428 (54%), Gaps = 18/428 (4%) Query: 12 ILAFVMLVTLVGCT-GKGNE---EVQKSDEQITLNFMWWGKPSRKEITLKVIELFEKENP 67 +L L+ L GC+ G NE E + +E + L WWG R ++T++ IELFE++ P Sbjct: 11 LLGVAFLLVLAGCSSGTSNEVDGEQEAGNEDVVLRVAWWGGQERHDMTIEAIELFEEKYP 70 Query: 68 NIKINTEDYPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNIL 127 +I+++ E + S ++L A PD++QMD + Y + L+ L + + Sbjct: 71 HIQVDPE-FTSWDNYWERLTTQAAGNNLPDVIQMDNSKLNEYSSRGLVIDLQSLIADGTI 129 Query: 128 DLSDVDKSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQ 187 +L DVD Y +G + + GSNAL Y+ M E+ GIQ L+ YT +D + MQ Sbjct: 130 NLDDVDDVYQDMNVQDGSVWAVSAGSNALAAIYNEEMLEEQGIQ-LEPGYTYEDFYEAMQ 188 Query: 188 QFKDKVQTPDFYPL---NAMFDLSY-WLRMKGESFYNKEKTGLGYTDATMIEYLTLIKTW 243 K+ + DFY NA +++ + + R GESFYN++ TGLG+ + T+I++LT +K Sbjct: 189 TLKENIDG-DFYGYDFGNAEYEMFFVYARQNGESFYNEDGTGLGFENQTLIDFLTFVKQM 247 Query: 244 LDLGLLNPGTASTTE-----ENPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQG 298 +D G+ P A T E E+ L + SN ++ + L +M LP+ G Sbjct: 248 VDDGVAPP-HALTLEYIQGGESLLGDQMAGAAMAASNGIIGLQQSTEHQLGLMLLPSLDG 306 Query: 299 AAAEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLS 358 GN+I+PSM +SS+SE +EAA F+ FLTND +AN ILK ERGVP+++KV E L+ Sbjct: 307 GV-HGNWIRPSMSYSISSHSEEQEAAALFIDFLTNDLQANEILKAERGVPISSKVREHLA 365 Query: 359 NEATEQGKEQYSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLYAGSITPEAAASSF 418 + + F++ +A +++P DP P ++ ++ + +++++ ++ E A F Sbjct: 366 PMVDGPIAKTFEFLELVADYTSPADPLPPPGESEVRGAFLRMIESVKYERLSIEEATEQF 425 Query: 419 RAQAEDVL 426 R +AE +L Sbjct: 426 RQEAEQIL 433 >BS_yesO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 412 Score = 204 bits (518), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 130/419 (31%), Positives = 216/419 (51%), Gaps = 27/419 (6%) Query: 23 GCTGKGNEEVQKSDEQITLNFMWWGKPSRKEITLKVIELFEKENPNIKINTEDYPSTGEV 82 GC+ EE E +TL WWG R + T KVIEL+EK+NP++ I E + + + Sbjct: 4 GCSA--GEEASGKKEDVTLRIAWWGGQPRHDYTTKVIELYEKKNPHVHIEAE-FANWDDY 60 Query: 83 AQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNILDLSDVDKSYLVSGQY 142 +KLA +A + PD++QMD + Y + +E L PY K +D+S +D++ L G+ Sbjct: 61 WKKLAPMSAAGQLPDVIQMDTAYLAQYGKKNQLEDLTPYTKDGTIDVSSIDENMLSGGKI 120 Query: 143 EGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQQFKDK--------VQ 194 + K YG +G N L + + + ++ G+ Q+ +T +D ++ ++K + Sbjct: 121 DNKLYGFTLGVNVLSVIANEDLLKKAGVSINQENWTWEDYEKLAYDLQEKAGVYGSNGMH 180 Query: 195 TPD-FYPLNAMFDLSYWLRMKGESFYNKEKTGLGYTDATM-IEY----LTLIKTWLDLGL 248 PD F+P Y+LR KGE FY ++ TGL Y D + ++Y L L+K Sbjct: 181 PPDIFFP--------YYLRTKGERFYKEDGTGLAYQDDQLFVDYFERQLRLVKAKTSPTP 232 Query: 249 LNPGTASTTEENPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLPTAQGAAAEGNFIKP 308 E++ + GK+A + SNQ + L + LP Q + +KP Sbjct: 233 DESAQIKGMEDDFIVKGKSAITWNYSNQYLGFARLTDSPLSLY-LPPEQ-MQEKALTLKP 290 Query: 309 SMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQLSNEATEQGKEQ 368 SM + SEHK+ A KF++F N++EAN ++KGERGVPV+ KVA+ + + E+ Sbjct: 291 SMLFSIPKSSEHKKEAAKFINFFVNNEEANQLIKGERGVPVSDKVADAIKPKLNEEETNI 350 Query: 369 YSFMKYIAKHSTPIDPPAPLSDAVIQNSLKLILKNLYAGSITPEAAASSFRAQAEDVLD 427 +++ +K+ + DPP P+ A + LK + ++PE AA +FR +A ++L+ Sbjct: 351 VEYVETASKNISKADPPEPVGSAEVIKLLKDTSDQILYQKVSPEKAAKTFRKKANEILE 409 >YPO1719 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 430 Score = 92.0 bits (227), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 88/357 (24%), Positives = 150/357 (42%), Gaps = 22/357 (6%) Query: 17 MLVTLVGCT-GKGNEEVQKSDEQITLNFMWWGKPSRKEITLKVIELFEKENPNIKINTED 75 +L TL+ T + + + + + L WWG R ++TLK +E F K++PNI + E Sbjct: 5 ILHTLIASTLALLSHQAFAAQDDVNLRMSWWGGNGRHQVTLKALEEFHKQHPNINVKAEY 64 Query: 76 YPSTGEVAQKLAMDTAEQKTPDLMQMDYNFAFNYVTHDLIEPLDPYVKSNILDLSDVDKS 135 G ++ +L A PD+MQ ++N+ + D D LDL+ D Sbjct: 65 TGWDGHLS-RLTTQIAGGTEPDVMQTNWNW-LPIFSKDGTGFFDLNNVKEQLDLAQFDPI 122 Query: 136 YLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQQFKDKVQT 195 L GK GIP+ A ++ A + + G L+ P T +L Q FK+K+ Sbjct: 123 ELQQTTVNGKLNGIPISVTARIFYFNDATWAKAG---LEYPKTWDELLNAGQVFKEKLGD 179 Query: 196 PDFYPL------NAMFDLSYWLRMKGESFYNKEKTGLGYTDATMIEYLTLIKTWLDLGLL 249 +YP+ SY + ++ YT +E+ T+ K +D ++ Sbjct: 180 -QYYPVVLEHQDTLALIRSYMTQKYNIPTVDEANKKFAYTPEQWVEFFTMYKKMVDSHVM 238 Query: 250 -------NPGTASTTEENPLAAGKTAFLQSVSNQMVSIGDEAGR--TLKIMNLPTAQGAA 300 + G ++ E P G+ A ++ + D + L + P A Sbjct: 239 PSSKYYASFGKSNMYEMKPWINGEWAGTYMWNSTITKYSDNLTKPAKLDLGPYPMLPDAK 298 Query: 301 AEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILKGERGVPVAAKVAEQL 357 G F KP+ + + ++H + + ++FL N KE L ERGVP++A QL Sbjct: 299 DAGLFFKPAQMLSIGKSTKHPKESAMLINFLLNSKEGVDALGLERGVPLSAAAVAQL 355 >BS_yurO [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 422 Score = 75.9 bits (185), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 83/346 (23%), Positives = 158/346 (45%), Gaps = 30/346 (8%) Query: 12 ILAFVMLVTLVGCTGKGNEEVQKSDEQITLNFMW-WGKPSRKEITLKVIELFEKENPNIK 70 I+A V ++T+ GC+ + + +D ++TL F W K K +V++ FEK++P+I Sbjct: 9 IIAAVSMLTIAGCSSQSSS----ADGKVTLKFFHRWPKEPEKSYFEEVVKEFEKDHPDID 64 Query: 71 INTEDYPSTGEVAQKLAMDTAEQKTPDL-MQMDYNFAFNYVTHDLIEPLDPYVKSNILDL 129 I E + K+ + PD+ FAF ++ + L Y K++ Sbjct: 65 IQIEAVLNDS-YKDKIKVMLGTTSPPDIYFSWSDEFAFKFIRGNKALDLSSYYKNDTDWS 123 Query: 130 SDVDKSYLVSGQYEGKQYGIPMGSNALGMAYDPAMFEQYGIQPLQDPYTIQDLQQTMQQF 189 S + +S + YE KQYG+P +A Y+ +F++ + P P T +L ++ Sbjct: 124 SQLVQSQITPFTYENKQYGVPWQMDAKSFFYNKDIFQKLNLDP---PKTWDELIDVSKKL 180 Query: 190 KDKVQTPDFYPLNAMFDLSYWL-----RMKGESFYNKE---KTGLGYTDATMIEYLTLIK 241 K+ TP + A + +S+++ RM E K+ KTG +TD ++ L ++ Sbjct: 181 KEHGYTPISFGTKATWTISHYIGTLNQRMVDEKTREKDYNAKTG-EFTDEGYVKALEKLQ 239 Query: 242 TWLDLGLLNPGTASTTEE---NPLAAGKTAFLQSVSNQMVSIGDEAGRTLKIMNLP--TA 296 + N S E +GK+A + + + ++ + L + P + Sbjct: 240 EL--MPYFNKHVNSVDHEYVRQQFKSGKSAMIYAETAEIKLV---EPVNLGMFPFPEISG 294 Query: 297 QGAAAEGNFIKPSMFIMVSSYSEHKEAAVKFVSFLTNDKEANAILK 342 Q ++E P F M+SS ++H + A++F+ FLT+ + ++K Sbjct: 295 QKGSSEALTGAPEGF-MISSRTKHPKEAMEFLQFLTSKRMGEKLVK 339 >Cgl1352 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 438 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 67/301 (22%), Positives = 127/301 (42%), Gaps = 21/301 (6%) Query: 44 MWWGK--PSRKEITLKVIELFEKENPNIKINTEDYPST-GEVAQKLAMDTAEQKTPDLMQ 100 +WW + K++ L++I FE ENP++K+ D + EV+QK + PDL+ Sbjct: 48 VWWSNHPANSKDVELELISRFEAENPDLKVQLVDAGANYAEVSQKFNAALSGGDLPDLVV 107 Query: 101 MDYNFAFNYVTHDLIEPLDPYVKSNILDLSDVDKSYLVSGQYEGKQYGIPMGSNALGMAY 160 + FN+ + +D K N +D S S +EG YG+P + + Y Sbjct: 108 LSDTEWFNFAINGATVNIDEVAKRNNIDTSTYVDSLFNDYSHEGGHYGLPFARSTVLFYY 167 Query: 161 DPAMFEQYGIQPLQDPYTIQDLQQTMQQFKDKVQTPDFYPLNAMFDLSYW-----LRMKG 215 + ++ + G++ + P + ++ + + ++ + + + + W + G Sbjct: 168 NKDLWAKAGLED-RGPESWEEFSEWGPKLQEAMDSGFAHGWGDATNYLSWTFEGPMWSLG 226 Query: 216 ESFYNKEKTGLGYTDATMIEYLTLIKTWLDLGLLNPGTASTTEENPLAAGKTAFLQSVSN 275 ++ ++ L T I + +K+ +D G T ST N A G + Sbjct: 227 GNYSEGWESRL--TTPETIRAVEWLKSTVDEGF---ATVSTDVTNEFATGLIGSCIQSTG 281 Query: 276 QMVSIGDEAGRTLKIMNL--PTAQGAAAEGNFIKPSMFIMVSSYSEHK-EAAVKFVSFLT 332 + S+ A + L PT +GA G + + S SE + + A+KF+ FLT Sbjct: 282 DLSSVAGAASFDWGVAALPNPTGEGACPTGG----AGLGIPSGISEQRQDNALKFIDFLT 337 Query: 333 N 333 N Sbjct: 338 N 338 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.130 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,278,120 Number of extensions: 1286377 Number of successful extensions: 3661 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 3654 Number of HSP's successfully gapped: 27 Length of query: 434 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 325 Effective length of database: 25,807,025 Effective search space: 8387283125 Effective search space used: 8387283125 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 117 (49.7 bits)