BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0043.p (169 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_def [J] COG0242 N-formylmethionyl-tRNA deformylase 147 7e-36 CAC1722 [J] COG0242 N-formylmethionyl-tRNA deformylase 145 3e-35 CAC2474 [J] COG0242 N-formylmethionyl-tRNA deformylase 144 5e-35 SMc01101 [J] COG0242 N-formylmethionyl-tRNA deformylase 132 2e-31 SP1549 [J] COG0242 N-formylmethionyl-tRNA deformylase 127 9e-30 >BS_def [J] COG0242 N-formylmethionyl-tRNA deformylase Length = 160 Score = 147 bits (371), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 80/150 (53%), Positives = 101/150 (67%) Query: 9 MAIRLIVKEPDEVLHKTAKTVTKITHNVQKLLDDMADTMYDAEGVGLAAPQVGILKRLIV 68 MA++ +V P EVL A+TVT ++KLLDDM DTM + +GVGLAAPQ+GILKR V Sbjct: 1 MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAV 60 Query: 69 VDADEEHGLIKLINPEIVSMEGEQFGPEGCLSIPGLNGDVRRAETVTVRGLDREGNEVTI 128 V+ ++ G I L+NPEI+ GEQ G EGCLS P + GDV RA+ V VR +R+G + Sbjct: 61 VEIGDDRGRIDLVNPEILEKSGEQTGIEGCLSFPNVYGDVTRADYVKVRAFNRQGKPFIL 120 Query: 129 TGSGLLARAFQHEIDHLNGVLFTDIAEKVY 158 G LARA QHE+DHL+GVLFT K Y Sbjct: 121 EARGFLARAVQHEMDHLDGVLFTSKISKYY 150 >CAC1722 [J] COG0242 N-formylmethionyl-tRNA deformylase Length = 150 Score = 145 bits (366), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 77/144 (53%), Positives = 95/144 (65%), Gaps = 2/144 (1%) Query: 9 MAIRLIVKEPDEVLHKTAKTVTKITHNVQKLLDDMADTMYDAEGVGLAAPQVGILKRLIV 68 MAIR I K DE+L K ++ V KI + L+DDM +TMY+A+GVGLAAPQVGILKRL+V Sbjct: 1 MAIRSIRKYGDELLRKKSRKVEKIDKRLLTLIDDMFETMYNADGVGLAAPQVGILKRLVV 60 Query: 69 VDADEEHGLIKLINPEIVSMEGEQFGPEGCLSIPGLNGDVRRAETVTVRGLDREGNEVTI 128 +D E G + LINPEI+ G+ EGCLSIP G+V R V + L+ +G E+ I Sbjct: 61 IDVGE--GPVVLINPEILETSGKAVDVEGCLSIPERQGEVERPTYVKAKALNEKGEEIVI 118 Query: 129 TGSGLLARAFQHEIDHLNGVLFTD 152 L ARA HE DHLNGVLF D Sbjct: 119 EAEDLFARAICHETDHLNGVLFVD 142 >CAC2474 [J] COG0242 N-formylmethionyl-tRNA deformylase Length = 150 Score = 144 bits (364), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 75/153 (49%), Positives = 106/153 (69%), Gaps = 6/153 (3%) Query: 9 MAIRLIVKEPDEVLHKTAKTVTKITHNVQKLLDDMADTMYDAE-GVGLAAPQVGILKRLI 67 MA+R I DE+L K ++ V + ++++LDDM DT+ + E G +AAPQVGILK+L+ Sbjct: 1 MALRQIRLSEDEILRKKSRPVEVVDDKIRQILDDMLDTLQNTENGAAIAAPQVGILKQLV 60 Query: 68 VVDADEEHGLIKLINPEIVSMEGEQFGPEGCLSIPGLNGDVRRAETVTVRGLDREGNEVT 127 V+ E+ +IKL+NP+IV EGEQ EGCLSIP + G ++R + VTV L+ G ++T Sbjct: 61 VIATGED--IIKLVNPKIVKKEGEQEVVEGCLSIPNVYGKLKRPKKVTVEALNENGEKIT 118 Query: 128 ITGSGLLARAFQHEIDHLNGVLFTDIAEKVYEY 160 +TG G LA+ F HEIDHL+G+LFTD+ V EY Sbjct: 119 LTGEGFLAKCFCHEIDHLDGILFTDL---VTEY 148 >SMc01101 [J] COG0242 N-formylmethionyl-tRNA deformylase Length = 178 Score = 132 bits (332), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 71/153 (46%), Positives = 94/153 (61%), Gaps = 4/153 (2%) Query: 9 MAIRLIVKEPDEVLHKTAKTVTKITHNVQKLLDDMADTMYDAEGVGLAAPQVGILKRLIV 68 M I+ ++ PD VL + + V I ++++L DDM +TMYDA G+GLAA Q+G+ KRL+V Sbjct: 5 MTIKPLIILPDPVLRQVSTPVETIDADIRRLADDMLETMYDAPGIGLAAIQIGVPKRLLV 64 Query: 69 VDAD---EEHGLIKLINPEIV-SMEGEQFGPEGCLSIPGLNGDVRRAETVTVRGLDREGN 124 +D EE + INP++V S E EGCLSIP +V R +TV +DREG Sbjct: 65 LDVTKEGEEKQPLVFINPKVVRSSEERSVYEEGCLSIPDYYAEVERPAAITVEYVDREGK 124 Query: 125 EVTITGSGLLARAFQHEIDHLNGVLFTDIAEKV 157 E + GLLA QHEIDHLNGVLF D K+ Sbjct: 125 EQAVEAEGLLATCLQHEIDHLNGVLFIDYISKL 157 >SP1549 [J] COG0242 N-formylmethionyl-tRNA deformylase Length = 136 Score = 127 bits (319), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 0/145 (0%), Positives = 0/145 (0%), Gaps = 1/145 (0%) Query: 9 MAIRLIVKEPDEVLHKTAKTVTKITHNVQKLLDDMADTMYDAEGVGLAAPQVGILKRLIV 68 M +++ + + +A+ + + Sbjct: 1 MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNL 60 Query: 69 VDADEEHG-LIKLINPEIVSMEGEQFGPEGCLSIPGLNGDVRRAETVTVRGLDREGNEVT 127 + L E G S + Sbjct: 61 GLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRLAYRDSKWQEQTITLTGFP 120 Query: 128 ITGSGLLARAFQHEIDHLNGVLFTD 152 Sbjct: 121 145 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,884,103 Number of extensions: 558915 Number of successful extensions: 1056 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 992 Number of HSP's successfully gapped: 59 Length of query: 169 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 70 Effective length of database: 26,960,365 Effective search space: 1887225550 Effective search space used: 1887225550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)