BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0042.p (273 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value fmt [J] COG0223 Methionyl-tRNA formyltransferase 223 2e-58 Zfmt [J] COG0223 Methionyl-tRNA formyltransferase 223 2e-58 ECs4153 [J] COG0223 Methionyl-tRNA formyltransferase 223 2e-58 aq_2131 [J] COG0223 Methionyl-tRNA formyltransferase 223 3e-58 >fmt [J] COG0223 Methionyl-tRNA formyltransferase Length = 301 Score = 223 bits (569), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 14/230 (6%), Positives = 43/230 (18%) Query: 1 MKIVFMGTPAFAVPSLQMLVKEGYEVVAVITQPDRPQGRKKTLTPSPVKEAAISLGLPVL 60 + + + + Q + + + A ++ + Sbjct: 5 KSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTP 64 Query: 61 QPERLRRPESVAELAAYEPDLIVTAAYGQILPKTVLELPQYGCVNVHGSLLPKYRGGAPI 120 + + I + + P V A Sbjct: 65 GLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVN 124 Query: 121 QRCIINGEKLTGVTLMYMAEGLDTGDMISRVEVPIGEEDTSGILFEKLSLAGCELLKTEM 180 + + + L ++A ++ D++ DT + K + Sbjct: 125 KVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDY 184 Query: 181 PRLAAGRVQATPQDDSEATYAPNLTREDERIDWSAGSQDIYNRIRGLVPF 230 + R A+ + + Sbjct: 185 ITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGL 234 >Zfmt [J] COG0223 Methionyl-tRNA formyltransferase Length = 315 Score = 223 bits (569), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 114/230 (49%), Positives = 150/230 (65%) Query: 1 MKIVFMGTPAFAVPSLQMLVKEGYEVVAVITQPDRPQGRKKTLTPSPVKEAAISLGLPVL 60 ++I+F GTP FA L L+ G+ VV V TQPDRP GR K L PSPVK A GLPV Sbjct: 5 LRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRGKKLMPSPVKVLAEEKGLPVF 64 Query: 61 QPERLRRPESVAELAAYEPDLIVTAAYGQILPKTVLELPQYGCVNVHGSLLPKYRGGAPI 120 QP LR E+ +A + D++V AYG ILPK VLE+P+ GC+NVHGSLLP++RG API Sbjct: 65 QPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEMPRLGCINVHGSLLPRWRGAAPI 124 Query: 121 QRCIINGEKLTGVTLMYMAEGLDTGDMISRVEVPIGEEDTSGILFEKLSLAGCELLKTEM 180 QR + G+ TGVT+M M GLDTGDM+ ++ PI EDTSG L++KL+ G + L T + Sbjct: 125 QRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPITAEDTSGTLYDKLAELGPQGLITTL 184 Query: 181 PRLAAGRVQATPQDDSEATYAPNLTREDERIDWSAGSQDIYNRIRGLVPF 230 +LA G + QD++ TYA L++E+ RIDWS + + IR P+ Sbjct: 185 KQLADGTAKPEVQDETLVTYAEKLSKEEARIDWSLSAAQLERCIRAFNPW 234 Score = 223 bits (569), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 114/230 (49%), Positives = 150/230 (65%) Query: 1 MKIVFMGTPAFAVPSLQMLVKEGYEVVAVITQPDRPQGRKKTLTPSPVKEAAISLGLPVL 60 ++I+F GTP FA L L+ G+ VV V TQPDRP GR K L PSPVK A GLPV Sbjct: 5 LRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRGKKLMPSPVKVLAEEKGLPVF 64 Query: 61 QPERLRRPESVAELAAYEPDLIVTAAYGQILPKTVLELPQYGCVNVHGSLLPKYRGGAPI 120 QP LR E+ +A + D++V AYG ILPK VLE+P+ GC+NVHGSLLP++RG API Sbjct: 65 QPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEMPRLGCINVHGSLLPRWRGAAPI 124 Query: 121 QRCIINGEKLTGVTLMYMAEGLDTGDMISRVEVPIGEEDTSGILFEKLSLAGCELLKTEM 180 QR + G+ TGVT+M M GLDTGDM+ ++ PI EDTSG L++KL+ G + L T + Sbjct: 125 QRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPITAEDTSGTLYDKLAELGPQGLITTL 184 Query: 181 PRLAAGRVQATPQDDSEATYAPNLTREDERIDWSAGSQDIYNRIRGLVPF 230 +LA G + QD++ TYA L++E+ RIDWS + + IR P+ Sbjct: 185 KQLADGTAKPEVQDETLVTYAEKLSKEEARIDWSLSAAQLERCIRAFNPW 234 >ECs4153 [J] COG0223 Methionyl-tRNA formyltransferase Length = 315 Score = 223 bits (569), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 114/230 (49%), Positives = 150/230 (65%) Query: 1 MKIVFMGTPAFAVPSLQMLVKEGYEVVAVITQPDRPQGRKKTLTPSPVKEAAISLGLPVL 60 ++I+F GTP FA L L+ G+ VV V TQPDRP GR K L PSPVK A GLPV Sbjct: 5 LRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRGKKLMPSPVKVLAEEKGLPVF 64 Query: 61 QPERLRRPESVAELAAYEPDLIVTAAYGQILPKTVLELPQYGCVNVHGSLLPKYRGGAPI 120 QP LR E+ +A + D++V AYG ILPK VLE+P+ GC+NVHGSLLP++RG API Sbjct: 65 QPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEMPRLGCINVHGSLLPRWRGAAPI 124 Query: 121 QRCIINGEKLTGVTLMYMAEGLDTGDMISRVEVPIGEEDTSGILFEKLSLAGCELLKTEM 180 QR + G+ TGVT+M M GLDTGDM+ ++ PI EDTSG L++KL+ G + L T + Sbjct: 125 QRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPITAEDTSGTLYDKLAELGPQGLITTL 184 Query: 181 PRLAAGRVQATPQDDSEATYAPNLTREDERIDWSAGSQDIYNRIRGLVPF 230 +LA G + QD++ TYA L++E+ RIDWS + + IR P+ Sbjct: 185 KQLADGTAKPEVQDETLVTYAEKLSKEEARIDWSLSAAQLERCIRAFNPW 234 >aq_2131 [J] COG0223 Methionyl-tRNA formyltransferase Length = 205 Score = 223 bits (567), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 0/238 (0%), Positives = 0/238 (0%), Gaps = 5/238 (2%) Query: 2 KIVFMGTPAFAVPSLQMLVKEGYEVVAVITQPDRPQGRKKTLTPSPVKEAAISLGLPVLQ 61 +++ + + ++ L + T R + ++ + Sbjct: 3 ELLAIVRKEKSYDVMKSLSE-AEIPYVSWTVKGRGKEGGLRYKGLIKEKVLMPFLPKRAF 61 Query: 62 PERLRRPESVAELAAYEPDLIVTAAYGQILPKTVLELPQYGCVNVHGSLLPKYRGGAPIQ 121 E A ++ V + P+ Sbjct: 62 LVF--PEEGKVNEAVNLIVESAHTGAYGDGKVFLINQEVGVMKLVKAVIRPEKVYEVIKA 119 Query: 122 RCIINGEKLTGVTLMYMAEGLDTGDMISRVEVPIGEEDTSGILFEKLSLAGCELLKTEMP 181 + +T ++ + + + + E + + K Sbjct: 120 LESEGFKAMTMWDVVGRGKEGGILVGGTPYDELAKTLIMVAVKDEDVDKVIQSITKAAHT 179 Query: 182 RLAAGRVQATPQDDSEATYAPNLTREDERIDWSAGSQDIYNRIRGLVPFSGAFTLWNG 239 Sbjct: 180 -- 235 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,796,993 Number of extensions: 830731 Number of successful extensions: 1397 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 1384 Number of HSP's successfully gapped: 28 Length of query: 273 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 168 Effective length of database: 26,268,361 Effective search space: 4413084648 Effective search space used: 4413084648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)