BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0034.p (585 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1928 [R] COG1461 Predicted kinase related to dihydroxyacetone... 457 e-128 L193593 [R] COG1461 Predicted kinase related to dihydroxyacetone... 422 e-118 L174523_1 [R] COG1461 Predicted kinase related to dihydroxyaceto... 126 1e-28 lin0599_1 [R] COG1461 Predicted kinase related to dihydroxyaceto... 115 2e-25 Rv2974c [R] COG1461 Predicted kinase related to dihydroxyacetone... 86 2e-16 >lin1928 [R] COG1461 Predicted kinase related to dihydroxyacetone kinase Length = 552 Score = 457 bits (1175), Expect = e-128, Method: Compositional matrix adjust. Identities = 262/584 (44%), Positives = 349/584 (59%), Gaps = 45/584 (7%) Query: 1 MVLAGAEKLQQHAEHVNSLNVFPVPDGDTGTNMNLTMTAGANELKKNNTGSVGQCAGVLS 60 M+ GAE L ++A+ V+SLNVFPVPDGDTGTNMNL+MT+GA E+ KN+ ++ L+ Sbjct: 14 MIALGAENLAKNADFVDSLNVFPVPDGDTGTNMNLSMTSGAEEVAKNDKETISAVGANLA 73 Query: 61 KGLLMGARGNSGVILSQLFRGLGRYAAQYDELNTQQFAAALQTGVDTAYKAVVKPVEGTI 120 KGLLMGARGNSGVILSQLFRG + + LN ++FA A GV+TAYKAV+KPVEGTI Sbjct: 74 KGLLMGARGNSGVILSQLFRGFSKSIENKETLNAEEFANAFVKGVETAYKAVMKPVEGTI 133 Query: 121 LTVAKEAARHAVYYARRTTDVTELMTEVLSRAKEALANTPELLPVLKQVGVVDSGGQGLV 180 LTVA+EAA+ V A ++ +M +L + K AL TP+LLPVLK+VGVVDSGGQGL+ Sbjct: 134 LTVAREAAKAGVLAANEHQEIELVMEAILKQGKVALEKTPDLLPVLKEVGVVDSGGQGLI 193 Query: 181 YIYEGFHQYLTGSAGTXXXXXXXXXXXXXXXXXXXXXXLTKHENXXXXXXXXXXXXXXTE 240 IYEGF+ LTG + E TE Sbjct: 194 IIYEGFYGVLTGKMAEAPDYMA-----------------SMGELINAEHHRHVQDFMSTE 236 Query: 241 DIEFLYDMEFFINRLLGGSVKTEFDEESFRKALSVNGDSIIVISDDETIKVHVHSKTPGE 300 DI F Y E + FDEE FR+ LS GDS++V +DDE +KVHVH + PGE Sbjct: 237 DIHFGYCTEIIVKINENKPGLKPFDEEQFRQDLSELGDSLLVAADDEVVKVHVHVEHPGE 296 Query: 301 VLNLALQYGEITQIHILNMREQHRDLLTAGMDIAPMPDLFADIPQEESSVQAPAVPPADD 360 VLN QYG + ++ + NMREQH +++ G D AP A + Sbjct: 297 VLNYGQQYGSLLKMKVENMREQHNEIV--GDDKAP----------------------AKE 332 Query: 361 MAQYGFIAVSSGEGISDIFRSLGVDAILSGGQTMNPSTEDFVNAISSISAKHIYILPNNS 420 A YG + VS+GEG+ +F S+GV +LSGGQTMNPSTED V AI S +A+ +++LPNN Sbjct: 333 KAPYGIVTVSAGEGLKKLFESMGVSIVLSGGQTMNPSTEDIVKAIESANAEQVFVLPNNK 392 Query: 421 NIVLAAQQAKELLEGEREITVIPSKSIPQGIAAAFAFQEEDAVETNTGNMLEAISQVKSG 480 NI +AA+QA ++L GE ++ +IP+K+IPQG+ A FQ E +N M EAI V SG Sbjct: 393 NIQMAAEQAAQIL-GEDKVQIIPTKTIPQGLTAVLTFQPEQDFTSNAEAMREAIQDVASG 451 Query: 481 QITNAVRDTVIEELEIKSGQYIGIANSKIVXXXXXXXXXXXXXXGSMLENGDEIVTVLTG 540 Q+T AVRDT +E + IK +IG+ KI +L++ EIVT++ G Sbjct: 452 QVTTAVRDTTVEGVVIKKDSFIGMVEGKIKVSRETLEEAAYETLEKLLDDDSEIVTIIFG 511 Query: 541 AESEDSVTDALENWLAEAYPQVEVEXXXXHNGGQPLYYYLFSVE 584 +++ + L + + EA+P VEVE H G QP+Y Y+F+VE Sbjct: 512 EDADAKKAELLADKITEAFPDVEVE---IHEGDQPVYPYIFAVE 552 >L193593 [R] COG1461 Predicted kinase related to dihydroxyacetone kinase Length = 535 Score = 422 bits (1086), Expect = e-118, Method: Compositional matrix adjust. Identities = 241/576 (41%), Positives = 338/576 (58%), Gaps = 41/576 (7%) Query: 9 LQQHAEHVNSLNVFPVPDGDTGTNMNLTMTAGANELKKNNTGSVGQCAGVLSKGLLMGAR 68 + + AE+VNSLNVFPVPDGDTGTNM +T+T GA ++ +VGQ A +LSKGLLMGAR Sbjct: 1 MNEQAEYVNSLNVFPVPDGDTGTNMGMTITNGAKDVASKPADTVGQVAQILSKGLLMGAR 60 Query: 69 GNSGVILSQLFRGLGRYAAQYDELNTQQFAAALQTGVDTAYKAVVKPVEGTILTVAKEAA 128 GNSGVILSQLFRG +Y Y+E++ AAALQ GV+ AYKAV+KPVEGTILTV++ AA Sbjct: 61 GNSGVILSQLFRGFAQYVKDYEEIDGIHLAAALQNGVEVAYKAVMKPVEGTILTVSRGAA 120 Query: 129 RHAVYYARRTTDVTELMTEVLSRAKEALANTPELLPVLKQVGVVDSGGQGLVYIYEGFHQ 188 A T D ++M L AK+ALA TP++LPVLK+VGVVDSGGQGLVYIYEGF Sbjct: 121 ELAKRKTDETDDAVKIMEAALEGAKKALAMTPDMLPVLKEVGVVDSGGQGLVYIYEGFLM 180 Query: 189 YLTGSAGTXXXXXXXXXXXXXXXXXXXXXXLTKHENXXXXXXXXXXXXXXTEDIEFLYDM 248 L G +HE+ T DI+F Y Sbjct: 181 ALNGE--------FVPETPVAELGAMDRMVNVEHESVANAS---------TADIKFGYCT 223 Query: 249 EFFINRLLGGSVKTEFDEESFRKALSVNGDSIIVISDDETIKVHVHSKTPGEVLNLALQY 308 E + G + + +D ++F+ L+ G+S++V+ D+E +KVHVH++ PG V+ L+Y Sbjct: 224 EIMVELGKGPTSRESYDHDNFQAYLAGIGNSLLVVDDEEVVKVHVHTEDPGLVMQEGLKY 283 Query: 309 GEITQIHILNMREQHRDLLTAGMDIAPMPDLFADIPQEESSVQAPAVPPADDMAQYGFIA 368 G + ++ + NMR Q+ + E ++ V + +G IA Sbjct: 284 GRLVKVKVDNMRLQNEGV-------------------AEKEAKSTNVSTSTSKKDWGLIA 324 Query: 369 VSSGEGISDIFRSLGVDAILSGGQTMNPSTEDFVNAISSISAKHIYILPNNSNIVLAAQQ 428 +S+GEG++DIFR +GV+ ++SGGQTMNPSTED + AI +++A+ + ILPNN NI +AA+ Sbjct: 325 ISAGEGLADIFREMGVNHVVSGGQTMNPSTEDILTAIEAVNAEKVIILPNNKNIFMAAKS 384 Query: 429 AKELLEGEREITVIPSKSIPQGIAAAFAFQEEDAVETNTGNMLEAISQVKSGQITNAVRD 488 A E+ E + VI S ++PQG A +F ++E N M +A+ VKSG +T A+RD Sbjct: 385 AAEV--AEIPVEVIESATVPQGFTALLSFDPTRSIEENKAAMTDALLDVKSGSVTTAIRD 442 Query: 489 TVIEELEIKSGQYIGIANSKIVXXXXXXXXXXXXXXGSMLENGDEIVTVLTGAESEDSVT 548 T ++ +EI +G+ N+KIV M++ EIV + G E + + Sbjct: 443 TNVDGIEIHKKDTLGMLNNKIVVSTRKMNDAIFAVFDQMIDEDSEIVAIYIGEEGKKANA 502 Query: 549 DALENWLAEAYPQVEVEXXXXHNGGQPLYYYLFSVE 584 D + L + YP++EVE H G QP+Y YLFSVE Sbjct: 503 DKIAKELEKKYPEIEVE---IHEGKQPVYPYLFSVE 535 >L174523_1 [R] COG1461 Predicted kinase related to dihydroxyacetone kinase Length = 326 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 97/348 (27%), Positives = 155/348 (44%), Gaps = 42/348 (12%) Query: 2 VLAGAEKLQQHAEHVNSLNVFPVPDGDTGTNMNLTMTAGANELKKNNTGSVGQCAGVLSK 61 ++AGA+K+ + +N +NVFPV DGDTG+N+ M + L NN S ++ Sbjct: 14 IIAGAQKVINGKDELNKINVFPVADGDTGSNLASLMQTIIDNLSNNNY-STNLLFDEVAS 72 Query: 62 GLLMGARGNSGVILSQLFRGLGRYAAQYDELNTQQFAAALQTGVDTAYKAVVKPVEGTIL 121 L+GARGNSG+I +Q + Y + Y E N + A V +AY ++++P EGTIL Sbjct: 73 AALIGARGNSGIIFAQYLNAVSDYYSVY-ETNLEILVLAFNRAVKSAYDSLIEPKEGTIL 131 Query: 122 TVAKEAARHAVYYARRTTDVTELMTEVLSRAKEALANTPELLPVLKQVGVVDSGGQGLVY 181 +V K A + + +T + + E A +AL T + +LK+ +VDSG +G Y Sbjct: 132 SVMKRWAEVLLITFKESTSFEKALLEAKGEAWKALKETQFQMSILKKNKLVDSGAKGFYY 191 Query: 182 IYEGFHQYLTGSAGTXXXXXXXXXXXXXXXXXXXXXXLTKHENXXXXXXXXXXXXXXTED 241 GF G + K N TED Sbjct: 192 FISGFTDAYCGEKVSKIQEGLIPS--------------IKELN--------------TED 223 Query: 242 IEFLYDMEFFINRLLGGSVKTEF-------DEESFRKALSVNGDSIIVISDDETIKVHVH 294 I+ E FI+ +EF E+ +++L GDS+++ + + IK+H+H Sbjct: 224 IK-----EHFISEAPKNRYCSEFIIKEPLKQEKDLKESLRRWGDSLVIAGNKKQIKIHIH 278 Query: 295 SKTPGEVLNLALQYGEITQIHILNMREQHRDLLTAGMDIAPMPDLFAD 342 + P +L L + G I + +M+ Q+ IA + D AD Sbjct: 279 TNHPENILALLEKTGTIIYQKVDDMQLQYEISQNRKSSIAIVTDSVAD 326 >lin0599_1 [R] COG1461 Predicted kinase related to dihydroxyacetone kinase Length = 320 Score = 115 bits (289), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 94/346 (27%), Positives = 159/346 (45%), Gaps = 43/346 (12%) Query: 2 VLAGAEKLQQHAEHVNSLNVFPVPDGDTGTNMNLTMTAGANELKKNNTGSVGQCAGVLSK 61 +L GA ++ + +N +N+FPV DGDTG+N+ M A + + S + ++ Sbjct: 13 MLNGAAEVISKKDELNRINIFPVADGDTGSNLASLMQAIIDNVSPREY-STKELLEEVAS 71 Query: 62 GLLMGARGNSGVILSQLFRGLGRYAAQYDELNT--QQFAAALQTGVDTAYKAVVKPVEGT 119 L+GARGNSG+I +Q + A Y L + A Q V AY+A++ P EGT Sbjct: 72 AALIGARGNSGMIFAQYLNAV---AESYHHLESTFDGLVEAFQKAVHKAYEALLDPKEGT 128 Query: 120 ILTVAK--EAARHAVYYARRTTDVTELMTEVLSRAKEALANTPELLPVLKQVGVVDSGGQ 177 IL+V K A Y R+ + L + + A++AL NT + +L++ +VDSG + Sbjct: 129 ILSVMKAWSEALAGTYEQERSFQQSLLNAQRV--AEQALINTEFQMAILRKNRLVDSGAK 186 Query: 178 GLVYIYEGFHQ-YLTGSAGTXXXXXXXXXXXXXXXXXXXXXXLTKHENXXXXXXXXXXXX 236 G Y G + Y G+ T T+ E+ Sbjct: 187 GFYYFIAGLTKAYCGGTVSTPNSYVEQE---------------TRQEH----------TH 221 Query: 237 XXTEDIEFLYDMEFFINRLLGGSVKTEFDEESFRKALSVNGDSIIVISDDETIKVHVHSK 296 T + + Y EF I + ++ R+ L+ GDS+++ +++ IK+H+H+ Sbjct: 222 FETSEPIYRYCSEFIIK-------EATISHQTLREVLAPKGDSLVIAGNEKQIKIHIHTN 274 Query: 297 TPGEVLNLALQYGEITQIHILNMREQHRDLLTAGMDIAPMPDLFAD 342 P E+L+L ++G++T + +MR Q+ IA + D AD Sbjct: 275 EPQEILSLLSEFGKMTYQKVDDMRLQYEVTKNPRAKIAIVTDSIAD 320 >Rv2974c [R] COG1461 Predicted kinase related to dihydroxyacetone kinase Length = 470 Score = 85.9 bits (211), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 121/530 (22%), Positives = 194/530 (36%), Gaps = 93/530 (17%) Query: 66 GARGNSGVILSQLFRGLGRYAAQ--------YDELNTQQFAAALQTGVDTAYKAVVK-PV 116 GARGNSGVILSQ+ RG+ A ++ AAL GV+ ++ V Sbjct: 3 GARGNSGVILSQILRGIAEVTATAAAASGAVLRAVDANALGAALWRGVELVVASMGGVEV 62 Query: 117 EGTILTVAKEAARHAVYYARRTTDVTELMTEVLSRAKEALANTPELLPVLKQVGVVDSGG 176 GTI++V + AA A + +T A AL TPE L VL G VD+GG Sbjct: 63 PGTIVSVLRAAAGAVDQCAHE--GLAGAVTAAGDAAVIALEKTPEQLDVLADAGAVDAGG 120 Query: 177 QGLVYIYEGFHQYLTGSAGTXXXXXXXXXXXXXXXXXXXXXXLTKHENXXXXXXXXXXXX 236 +GL+ + + + G A +E Sbjct: 121 RGLLVLLDALRSTICGQA----------------------PARAVYEPSPRALPTDTATQ 158 Query: 237 XXTEDIEFLYDMEFFINRLLGGSVKTEFDEESFRKALSVNGDSI-IVISDDETIKVHVHS 295 E +Y + +V + R L G+S+ I + ++ VHVH+ Sbjct: 159 RPAPQFEVMYLL----------AVCDAAAADQLRDRLKELGESVAIAAAPPDSYSVHVHT 208 Query: 296 KTPGEVLNLALQYGEITQIHILNMREQHRDLLTAGMDIAPMPDLFADIPQEESSVQAPAV 355 G + L G +++I I + T+G+ Sbjct: 209 DDAGAAVEAGLAVGRVSRIVISALGSG-----TSGL------------------------ 239 Query: 356 PPADDMAQYGFIAVSSGEGISDIFRSLGVDAILSGGQTMNP----STEDFVNAISSISAK 411 P +AV G+G +++F G + G + P S V A+ A Sbjct: 240 PAGGWTRGRAVLAVVDGDGAAELFAGEGACVLRPGPDAVTPAADISAHQLVRAVVDTGAA 299 Query: 412 HIYILPNNSNIVLAAQQAKELLEGER-------EITVIPSKSIPQGIAAAFAFQEEDAVE 464 H+ +LPN A+EL+ G ++ +P+ S+ QG+ AA A + Sbjct: 300 HVMVLPNGY------VAAEELVAGCTAAIGWGVDVVPVPTGSMVQGL-AALAVHDAARQA 352 Query: 465 TNTG-NMLEAISQVKSGQITNAVRDTVIEELEIKSGQYIGIANSKIVXXXXXXXXXXXXX 523 + G +M A + G + A + + K G +GIA +++ Sbjct: 353 VDDGYSMARAAGASRHGSVRIATQKALTWAGTCKPGDGLGIAGDEVLIVADDVAAAAIGL 412 Query: 524 XGSMLENGDEIVTVLTGAESEDSVTDALENWLAEAYPQVE-VEXXXXHNG 572 +L +G ++VTVL GA + V LE + + +P E V H G Sbjct: 413 VDLLLASGGDLVTVLIGAGVTEDVAVVLERHVHDHHPGTELVSYRTGHRG 462 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,715,606 Number of extensions: 1532355 Number of successful extensions: 2681 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2666 Number of HSP's successfully gapped: 15 Length of query: 585 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 473 Effective length of database: 25,461,023 Effective search space: 12043063879 Effective search space used: 12043063879 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 119 (50.4 bits)