BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0033.p (286 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yviA [S] COG1307 Uncharacterized protein conserved in bacteria 157 2e-38 lin2658 [S] COG1307 Uncharacterized protein conserved in bacteria 151 1e-36 BS_yitS [S] COG1307 Uncharacterized protein conserved in bacteria 144 2e-34 CAC1624 [S] COG1307 Uncharacterized protein conserved in bacteria 143 3e-34 SPy1936 [S] COG1307 Uncharacterized protein conserved in bacteria 142 5e-34 >BS_yviA [S] COG1307 Uncharacterized protein conserved in bacteria Length = 281 Score = 157 bits (396), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 94/280 (33%), Positives = 155/280 (55%), Gaps = 7/280 (2%) Query: 6 IVTDSTSDIPPTMAEALGIEVVPLTLMFGEEAFRDNVDMTPEQFYEXXXXXXXXXXXXXX 65 +VTDST+ IP M E I ++PL ++F EE +R+ +++ + FYE Sbjct: 5 VVTDSTAYIPKEMREQHQIHMIPLQVVFREETYREEIELDWKSFYEEVKKHNELPTTSQP 64 Query: 66 XXVEYMNVYQGIQERYPGCRILSFHISSGLSGTYQSAVLAKSMLEEKGESITVVDSLSAS 125 E + +Y+ + + Y ++S H+SSG+SGT+ SA A SM++ + DS + Sbjct: 65 PIGELVALYEELGKSYDA--VISIHLSSGISGTFSSAAAADSMVDNI--DVYPFDSEISC 120 Query: 126 YGFGFMVVEAARLAAEG-HDPEEILAQAERLRQSRKLYFLVDTLEYLQKGGRIGKASAFL 184 GF ++AA L G PE+I+ + E ++++ + YF+VD L +LQ+GGR+ A AF+ Sbjct: 121 LAQGFYALKAAELIKNGASSPEDIIKELEEMKKTVRAYFMVDDLAHLQRGGRLSSAQAFI 180 Query: 185 GTLLNIKPILSIDAEGIIYAVEKVRGRKKAVARMIELFKQDLPGVDKINVAVGHTAEPAS 244 G+LL +KPIL D + +I EK+R RKKA++R+ EL +D + AV H Sbjct: 181 GSLLKVKPILHFDNK-VIVPFEKIRTRKKAISRIYELLDEDASKGLPMRAAVIHANREEE 239 Query: 245 GEEFLKELAGHFTLEEKVLTNVGPVVGSHVGNGTLAVFIW 284 + ++EL+ + E + G V+G+H+G G L + W Sbjct: 240 AAKIIEELSAKYPHVEFYNSYFGAVIGTHLGEGALGI-CW 278 >lin2658 [S] COG1307 Uncharacterized protein conserved in bacteria Length = 283 Score = 151 bits (381), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 89/285 (31%), Positives = 160/285 (56%), Gaps = 6/285 (2%) Query: 1 MNRTV-IVTDSTSDIPPTMAEALGIEVVPLTLMFGEEAFRDNVDMTPEQFYEXXXXXXXX 59 MN + +VTDST+ +P + E L I VVPL+++ +++R+ +++ FY+ Sbjct: 1 MNEKIAVVTDSTTYLPDEVKEQLRINVVPLSVIIDGKSYREGEELSTADFYKKVKEAENF 60 Query: 60 XXXXXXXXVEYMNVYQGIQERYPGCRILSFHISSGLSGTYQSAVLAKSMLEEKGESITVV 119 E++ +++ ++E+ ++S H+SSG+SGT+Q+A A +++ G ++ Sbjct: 61 PTSSQPAPGEFIQLFEKLKEQGFDT-VISIHLSSGISGTFQNAASAGELID--GLNVVAY 117 Query: 120 DSLSASYGFGFMVVEAARLAAEGHDPEEILAQAERLRQSRKLYFLVDTLEYLQKGGRIGK 179 DS + G V+AA +A E I+++ +++++++ YF+VD L LQ+GGR+ Sbjct: 118 DSELSCMAQGMFAVKAAEMALANEPLERIISELDKIKKAQDAYFMVDDLNNLQRGGRLNG 177 Query: 180 ASAFLGTLLNIKPILSIDAEGIIYAVEKVRGRKKAVARMIELFKQDLPGVDKINVAVGHT 239 A A +G+LL IKPIL + + I+ EKVR +KKA+ R+ ++ + + + V H Sbjct: 178 AQALVGSLLQIKPILHFNDKQIVL-FEKVRTQKKALKRIEDILEVAIKNKNAEKAYVIHG 236 Query: 240 AEPASGEEFLKELAGHFTLEEKVLTNVGPVVGSHVGNGTLAVFIW 284 +P GE + K L F E L+ GPV+G+H+G G L + W Sbjct: 237 NDPEKGETWRKHLESKFPEVEFELSYFGPVIGTHLGEGALGL-TW 280 >BS_yitS [S] COG1307 Uncharacterized protein conserved in bacteria Length = 283 Score = 144 bits (363), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 93/279 (33%), Positives = 142/279 (50%), Gaps = 6/279 (2%) Query: 6 IVTDSTSDIPPTMAEALGIEVVPLTLMFGEEAFRDNVDMTPEQFYEXXXXXXXXXXXXXX 65 ++ DS +D+P + E GI +PL + G++ F D V + +Q +E Sbjct: 5 LIADSATDLPRSYFEEKGIGFIPLRVSLGDKEFEDAVTIHADQIFEAMQNGETPKTSQAS 64 Query: 66 XXVEYMNVYQGIQERYPGCRILSFHISSGLSGTYQSAVLAKSMLEEK--GESITVVDSLS 123 NV+ +Q G L SSGLSGTYQ+AV+ + ++E+ + V+DS Sbjct: 65 PQT-IKNVF--LQYAETGDPALYIAFSSGLSGTYQTAVMIANEVKEEFPDFDLRVIDSKC 121 Query: 124 ASYGFGFMVVEAARLAAEGHDPEEILAQAERLRQSRKLYFLVDTLEYLQKGGRIGKASAF 183 AS G+G V AA L G+ +EI + + F VD L YL +GGRI K SAF Sbjct: 122 ASLGYGLAVRHAADLCINGNTIQEIETSVKNFCSQLEHIFTVDDLTYLARGGRISKTSAF 181 Query: 184 LGTLLNIKPILSIDAEGIIYAVEKVRGRKKAVARMIELFKQDLPGVDKINVAVGHTAEPA 243 +G LLNIKP+L ++ +G + +EK+RG+KK R+IEL K+ V + + A Sbjct: 182 VGGLLNIKPLLQME-DGKLVPLEKIRGQKKLFKRIIELMKERGDDWSNQTVGISYAANKE 240 Query: 244 SGEEFLKELAGHFTLEEKVLTNVGPVVGSHVGNGTLAVF 282 + + F +E ++ + +GSH G GTLA+F Sbjct: 241 KATDMKHLIEEAFKPKEIIMHPISSAIGSHAGPGTLAIF 279 >CAC1624 [S] COG1307 Uncharacterized protein conserved in bacteria Length = 280 Score = 143 bits (361), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 89/287 (31%), Positives = 157/287 (54%), Gaps = 12/287 (4%) Query: 1 MNRTVIVTDSTSDIPPTMAEALGIEVVPLTLMFGEEAFRDNVDMTPEQFYEXXXXXXXXX 60 M + ++TDSTSD+ M E I+V+PL +++ ++ + D V ++P + Y+ Sbjct: 1 MEKIALITDSTSDVDKDMIEKYDIKVLPLRIIYKDKEYIDRVTISPREVYDNLHIEVPST 60 Query: 61 XXXXXXXVEYMNVYQGIQ-ERYPGCRILSFHISSGLSGTYQSAVLAKSMLEEKGESIT-- 117 +E ++Y+ ++ E Y ++ ISS LSGTY S ++ E E+I Sbjct: 61 SLPSMGDME--SLYEKLEAEGY--THVIGVTISSNLSGTYNSL----KLVSEGHENIKSF 112 Query: 118 VVDSLSASYGFGFMVVEAARLAAEGHDPEEILAQAERLRQSRKLYFLVDTLEYLQKGGRI 177 + DS S + G G ++ E + +G + + I+ + ++R++ K+Y++VDTL+YL KGGRI Sbjct: 113 IFDSRSITMGEGAIIKECGEMILQGKNFDYIVEKMPKMRENIKVYYIVDTLKYLIKGGRI 172 Query: 178 GKASAFLGTLLNIKPILSIDAEGIIYAVEKVRGRKKAVARMIELFKQDLPGVDKINVAVG 237 GK S +G LL+IKPI+SI+ EG+ Y K +G+K+A+ ++IE+ ++ L ++ V Sbjct: 173 GKVSGTVGQLLDIKPIISINEEGVYYTHCKAKGKKQAIKKLIEILRRTLSE-KHCDIWVM 231 Query: 238 HTAEPASGEEFLKELAGHFTLEEKVLTNVGPVVGSHVGNGTLAVFIW 284 H ++ ++G + + PV G H G G L V I+ Sbjct: 232 HGGAYDEIDKLKDLVSGIENINNLNSGEISPVAGVHTGPGLLGVVIF 278 >SPy1936 [S] COG1307 Uncharacterized protein conserved in bacteria Length = 286 Score = 142 bits (358), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 93/281 (33%), Positives = 150/281 (53%), Gaps = 8/281 (2%) Query: 6 IVTDSTSDIPPTMAEALGIEVVPLTLMFGEEAFRD-NVDMTPEQFYEXXXXXXXXXXXXX 64 I+TDST+D+ T AE I ++ LT++ E + + + Sbjct: 5 IMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNRISSDYLLKKMKAGSHPQTSQ 64 Query: 65 XXXVEYMNVYQGIQERYPGCRILSFHISSGLSGTYQSAVLAKSMLEEK--GESITVVDSL 122 E+ V++ L+F SS LSGTYQSA++A+ ++ E I +VD+L Sbjct: 65 INVGEFEKVFREHARNNKALLYLAF--SSVLSGTYQSALMARDLVREDYPDAVIEIVDTL 122 Query: 123 SASYGFGFMVVEAARLAAEGHDPEEILAQAERLRQSRKLYFLVDTLEYLQKGGRIGKASA 182 +A+ G G++ + AA G + E E + + YFLVD L +L +GGR+ K SA Sbjct: 123 AAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLRTYFLVDDLFHLMRGGRLSKGSA 182 Query: 183 FLGTLLNIKPILSIDAEGIIYAVEKVRGRKKAVARMIELFKQDLPGVDKINVAVGHTAEP 242 FLG+L +IKP+L ID EG + + K+RGR+KA+ M+ ++D+ V V +T++ Sbjct: 183 FLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEMVAQVEKDIADS---TVIVSYTSDQ 239 Query: 243 ASGEEFLKELAGHFTLEEKVLTNVGPVVGSHVGNGTLAVFI 283 S E+ +EL H + + ++ +GPV+ +HVG TLAVF+ Sbjct: 240 GSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTLAVFV 280 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,883,229 Number of extensions: 767536 Number of successful extensions: 1653 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 1580 Number of HSP's successfully gapped: 35 Length of query: 286 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 181 Effective length of database: 26,268,361 Effective search space: 4754573341 Effective search space used: 4754573341 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)