BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0026.p (415 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhaU [P] COG0475 Kef-type K+ transport systems, membrane comp... 358 9e-99 DR1149 [P] COG0475 Kef-type K+ transport systems, membrane compo... 94 3e-19 AF1245 [P] COG0475 Kef-type K+ transport systems, membrane compo... 74 3e-13 PA5529 [P] COG0475 Kef-type K+ transport systems, membrane compo... 70 6e-12 aq_2030 [P] COG0475 Kef-type K+ transport systems, membrane comp... 57 5e-08 >BS_yhaU [P] COG0475 Kef-type K+ transport systems, membrane components Length = 444 Score = 358 bits (919), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 15/407 (3%), Positives = 48/407 (11%), Gaps = 3/407 (0%) Query: 1 MNHYIVFEXXXXXXXXXXXXXXSTKLRFSVIPFYILIGMAVGPHAFKIWHLDFRFIESAA 60 + + I ++ + +I SA Sbjct: 4 DTV-DEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAF 62 Query: 61 LIEFMGRIGVLFLLFYLGLEFSVGRLVKAGRSIVTGGTIYILINFTLGLVLGWGLGFPLE 120 ++ L V S+ T ++ + + + Sbjct: 63 GWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFML 122 Query: 121 EILXXXXXXXXXXXXXXXKVLVDLKRTANPETEMILGIIMFEDVFLAVYIXXXXXXXXXX 180 + + + L Sbjct: 123 GFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFT 182 Query: 181 XXXXXXXXXXXXXXXXYMLALLVIGRKLVPWLNRVLNIRSGEXXXXXXXXXXXXXXXXSE 240 N + Sbjct: 183 VMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHY 242 Query: 241 TIHXXXXXXXXXXXXXXXXTEHVKRIEKLVIPXXXXXXXXXXXXXGLTIDPLSLGGDALW 300 + P + + Sbjct: 243 IKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPAL-CGFA 301 Query: 301 YSLAAVIITLIGNFAAGMLAGRSAGLSPRSSANIGLTIVSRGEFSIIMANLGKAGGLLAI 360 + + + L I+ + + + A Sbjct: 302 GGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGK 361 Query: 361 LQPFAAMYVLILAILGPLLTKESKTIYKLLDKVFKFEKRHLARSSRQ 407 V+ ++ + + Sbjct: 362 GFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIV-TPLVIGYLVS 407 >DR1149 [P] COG0475 Kef-type K+ transport systems, membrane components Length = 383 Score = 94.4 bits (233), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 104/363 (28%), Positives = 151/363 (41%), Gaps = 25/363 (6%) Query: 25 KLRFSVIPFYILIGMAVGPHAFKIWHLDF---RFIESAALIEFMGRIGVLFLLFYLGLEF 81 +L + IP Y++ G+ +G HLD +FI + A IG + LLF LGLE+ Sbjct: 25 RLGITPIPLYLMAGIGLG----TFMHLDDTAEKFIHTGA------EIGAILLLFILGLEY 74 Query: 82 SVGRL---VKAGRSIVTGGTIYILINFTLGLVLGWGLGFPLEEILXXXXXXXXXXXXXXX 138 + L +KA R I G I + +NFT G+V G LG P + Sbjct: 75 TSDELRTNLKANRQI---GLIDLALNFTPGVVAGALLGLPPLAWVLLGGITFLTSSGIAS 131 Query: 139 KVLVDLKRTANPETEMILGIIMFEDVFLAVYIXXXXXXXXXXXXXXXXXXXXXXXXXXYM 198 K+L DL R N ET +IL + + EDV +A Y+ + Sbjct: 132 KILSDLGRLGNRETPIILAVCVIEDVVMAFYLPVVAALLIGGSLLSVGVNLALALTAFGV 191 Query: 199 LALLVIGRKLVPWLNRVLNIRSGEXXXXXXXXXXXXXXXXSETIHXXXXXXXXXXXXXXX 258 L + V L+RVLN+ S E ++ + Sbjct: 192 TFFLAMRYGHV--LSRVLNVPSDETLLLGVLGLVLVVAGAADLLK-VSAAIGAFLVGIAL 248 Query: 259 XTEHVKRIEKLVIPXXXXXXXXXXXXXGLTIDPLSLGGDALWYSLAAVIITLIGNFAAGM 318 E R + + P GL +D + L +L AV +T I F G Sbjct: 249 SGEVAHRARQQIEPLRDLFAAVFFLFFGLQLDLAEVPEVLLPAALLAV-VTSITKFLVGW 307 Query: 319 LAGRSAGLSPRSSANIGLTIVSRGEFSIIMANLGKAGGLLAILQPFAAMYVLILAILGPL 378 R AG+ R G T+++RGEFSI++A LG GL +L P AA+YVL+ AI+GP+ Sbjct: 308 YGARRAGVQSRGRYRAGTTLIARGEFSILIAGLGL--GLAPVLGPLAAVYVLLTAIIGPV 365 Query: 379 LTK 381 L + Sbjct: 366 LAR 368 >AF1245 [P] COG0475 Kef-type K+ transport systems, membrane components Length = 338 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 48/144 (33%), Positives = 76/144 (52%), Gaps = 11/144 (7%) Query: 23 STKLRFSVIPFYILIGMAVGPHAFKIWHLDFRFIESAALIEFMGRIGVLFLLFYLGLEFS 82 S LR + IP YIL+GMA+G WHL + +EF+G++G++FLLFY+GL+ Sbjct: 17 SRMLRITPIPAYILVGMALGKAG---WHL--------SEVEFLGKLGIVFLLFYVGLKIP 65 Query: 83 VGRLVKAGRSIVTGGTIYILINFTLGLVLGWGLGFPLEEILXXXXXXXXXXXXXXXKVLV 142 + K + + GTI L NF LVL + +GF L + K+LV Sbjct: 66 PSTIKKTWQRTLKSGTIDFLFNFLPPLVLLYLIGFDLRDAFILASAIYISSSAINLKMLV 125 Query: 143 DLKRTANPETEMILGIIMFEDVFL 166 D ++ E+++ +++FED+ L Sbjct: 126 DDRKLVFTFAEVVVLLMVFEDIVL 149 >PA5529 [P] COG0475 Kef-type K+ transport systems, membrane components Length = 585 Score = 70.1 bits (170), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 91/385 (23%), Positives = 142/385 (36%), Gaps = 19/385 (4%) Query: 25 KLRFSVIPFYILIGMAVGPHAFKIWHLDFRFIESAALIEFMGRIGVLFLLFYLGLEFSVG 84 +LR V+ YI+ G +GPH F I I+ + +GV+FL+F LGLEFS+ Sbjct: 26 RLRQPVVLGYIVAGFIIGPHT-----PPFGLIHDEDTIKTLAELGVIFLMFCLGLEFSLR 80 Query: 85 RLVKAGRSIVTGGTIYILINFTLGLVLGWGLGFPLEEILXXXXXXXXXXXXXXXKVLVDL 144 +L + G + + I++ +G +G + + L K L DL Sbjct: 81 KLFQVGATAFIAAFLEIVLMIWIGFEIGRFFEWSTMDSLFLGAILAISSTTIIVKALGDL 140 Query: 145 KRTANPETEMILGIIMFEDVFLAVYIXXXXXXXXXXXXXXXXXXXXXXXXXXYMLALLVI 204 K ++I G+++ ED+ I +M+ LV+ Sbjct: 141 KMKNERFAQLIFGVLIVEDILGIGIIALLSGIAVSGSVETGEVFSTVGKLSLFMIVALVV 200 Query: 205 GRKLVP-WLNRVLNIRSGEXXXXXXXXXXXXXXXXSETIHXXXXXXXXXXXXXXXXTEHV 263 G LVP L V S E + + + Sbjct: 201 GILLVPRLLAYVAKFESNEMLLVTVLGLCFGFCLLVVKLEYSMVLGAFLIGAIMAESRQL 260 Query: 264 KRIEKLVIPXXXXXXXXXXXXXGLTIDPLSLGGDALWYSLAAVIITLIGNFAAGMLAGRS 323 +IE+L+ P GL IDP L A +L V + L F+ GM Sbjct: 261 VQIERLIEPVRDMFSAIFFVAIGLLIDPKILVEYAWPIALITVAVVLGKMFSCGM-GAFI 319 Query: 324 AGLSPRSSANIGLTIVSRGEFSIIMANLGKAGGLLA-ILQPFAAMYVLILAILGPLLTKE 382 AG R+S +G+ + GEFS I+A LG + + L P A I +L P L + Sbjct: 320 AGNDGRTSLRVGMGLSQIGEFSFIIAALGMTLKVTSDFLYPVAVAVSAITTLLTPYLIRA 379 Query: 383 SKTIYKLLDKVFKFEKRHLARSSRQ 407 + + +HLA + Q Sbjct: 380 ADPL-----------SQHLANAMPQ 393 >aq_2030 [P] COG0475 Kef-type K+ transport systems, membrane components Length = 393 Score = 57.4 bits (137), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 5/105 (4%), Positives = 15/105 (14%), Gaps = 7/105 (6%) Query: 63 EFMGRIGVLFLLFYLGLEFSVGRLVKAGRSIV---TGGTIYILINFTLGLVLGWGLGFPL 119 + + S + ++ + G L Sbjct: 59 THPLFKPREEKRVSVVVFTSDLSF----TRELCNKIFKSVPEFRETEFIIFGGKCRKKDL 114 Query: 120 EEILXXXXXXXXXXXXXXXKVLVDLKRTANPETEMILGIIMFEDV 164 E L ++ ++ Sbjct: 115 FERLKPKVISNTFTKLVDYSKILKTFEELFERFASKELDAVYVVS 159 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.145 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,603,135 Number of extensions: 808644 Number of successful extensions: 2181 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 2190 Number of HSP's successfully gapped: 11 Length of query: 415 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 306 Effective length of database: 25,807,025 Effective search space: 7896949650 Effective search space used: 7896949650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)