BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0025.p (164 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yhaT [P] COG0490 Putative regulatory, ligand-binding protein ... 167 5e-42 BS_yrvC [P] COG0490 Putative regulatory, ligand-binding protein ... 137 5e-33 >BS_yhaT [P] COG0490 Putative regulatory, ligand-binding protein related to C-terminal domains of K+ channels Length = 54 Score = 167 bits (424), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 0/164 (0%), Positives = 0/164 (0%) Query: 1 MNYRESDLPGIGKKFVMQTRSGDKLVVIVHDDGRRELYHFEYDDPDQSISMITLDDDEAR 60 Sbjct: 1 60 Query: 61 YISAIVGGITYKPKALESIEVALDDLIIEWYRLEQDFKCIGHSIGELDXXXXXXXXXXXX 120 Sbjct: 61 120 Query: 121 XEKNHQKHINPGPEVLLPAECTVVAAGERHQHKQFKHILRNGCG 164 Sbjct: 121 164 >BS_yrvC [P] COG0490 Putative regulatory, ligand-binding protein related to C-terminal domains of K+ channels Length = 165 Score = 137 bits (346), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 68/148 (45%), Positives = 96/148 (64%) Query: 1 MNYRESDLPGIGKKFVMQTRSGDKLVVIVHDDGRRELYHFEYDDPDQSISMITLDDDEAR 60 M RES+LPGIG+K M TR+ DK+ +I+H DGRRELY+F+ +D ++ ++ + DD EAR Sbjct: 1 MRVRESELPGIGQKVEMITRNRDKISIIIHHDGRRELYYFDENDHEECVASVQFDDAEAR 60 Query: 61 YISAIVGGITYKPKALESIEVALDDLIIEWYRLEQDFKCIGHSIGELDXXXXXXXXXXXX 120 +SAI+GG+ YKPKALE +E ALDDLIIEW + E I +IG+L+ Sbjct: 61 QMSAILGGMAYKPKALEQVESALDDLIIEWCKAEAGAPAIHQTIGDLNVGQEYHVTVIAI 120 Query: 121 XEKNHQKHINPGPEVLLPAECTVVAAGE 148 +KN K +NP E ++ T+V +GE Sbjct: 121 VKKNQDKQLNPSSETVIDEGDTLVISGE 148 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,908,493 Number of extensions: 447405 Number of successful extensions: 656 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 656 Number of HSP's successfully gapped: 2 Length of query: 164 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 66 Effective length of database: 27,075,699 Effective search space: 1786996134 Effective search space used: 1786996134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)