BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0010.p (263 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA2260 [IQR] COG1028 Dehydrogenases with different specificities... 117 2e-26 XF1726 [IQR] COG1028 Dehydrogenases with different specificities... 116 3e-26 CC0094 [IQR] COG1028 Dehydrogenases with different specificities... 115 5e-26 STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 115 7e-26 STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase 115 7e-26 >SA2260 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 272 Score = 117 bits (293), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 87/259 (33%), Positives = 125/259 (48%), Gaps = 16/259 (6%) Query: 4 KKLEXXXXXXXXXXXXXXXXXXLAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIE 63 K+LE +A A+ GA V +D +E V K Sbjct: 2 KRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSETVDK-IKSNGDNAKAYN 60 Query: 64 CDIAEPQQVENAVKQAAGQWGRLDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFA 123 DI++ QQV + V Q+GR+D++F NAG++ A I I+ +D+ MN+++RGT Sbjct: 61 VDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEYPIDVYDKIMNVDMRGTIL 120 Query: 124 TVKYAIPYLKANGGSILINSSINGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIR 183 K +P + GGSI+ SS +G ++ S Y+ K F K A+E + IR Sbjct: 121 MTKMMLPLMMNQGGSIVNTSSFSGQA--EDLYRSGYNAAKGAVINFTKSIAIEYGRDGIR 178 Query: 184 VNAICPGAITTN-IDDNTYPSED-----LKEVQIAVEFPDGGQPLEKGPGRPDQVARLAL 237 NAI PG I T +D T SED +E Q + PL + G+P++VA+L + Sbjct: 179 SNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWM------TPLGR-LGKPEEVAKLVV 231 Query: 238 FLASDDSDHITGTEIYCDG 256 FLASDDS ITG I DG Sbjct: 232 FLASDDSSFITGETIRIDG 250 >XF1726 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 255 Score = 116 bits (291), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 68/229 (29%), Positives = 110/229 (48%), Gaps = 7/229 (3%) Query: 28 FARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGRLD 87 FA+ GA V + DR E A + + CD+++ QV V++ ++GRLD Sbjct: 29 FAQGGASVVLADRDGERAAQEAAAIVAEGGTAIGLACDVSDEAQVAALVEKTVAEYGRLD 88 Query: 88 IVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYLKANGGSILINSSING 147 F NAG+ + P E +D+ + INLRG + +KY + ++ G +++N++ G Sbjct: 89 FAFNNAGVMAKIAPFAEATSEEFDRVIGINLRGVWLCMKYELQQMQRQGSGVIVNNASVG 148 Query: 148 NRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSEDLK 207 + N+G AY +K G + AALE + IRVNA+ PG I T I + D Sbjct: 149 -ALTGNVGIGAYIASKHGVLGLTRAAALEYCKKGIRVNAVNPGTIDTQIARDVVGGSDEA 207 Query: 208 EVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDG 256 ++ P G GRP+++A L+L S ++ G + DG Sbjct: 208 FAKLGASAPIG------RAGRPEEIASAVLWLCSPGGSYVIGEGLTVDG 250 >CC0094 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 296 Score = 115 bits (289), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 78/199 (39%), Positives = 107/199 (53%), Gaps = 9/199 (4%) Query: 63 ECDIAEPQQVENAVKQAAGQWGRLDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTF 122 CD+ + + A +G LDI+F NAG G + + E+WD+T + +RG Sbjct: 59 RCDVTADDDLAKTMALAESSFGGLDILFNNAGHGGTPASVPELTAEAWDKTFALLVRGPA 118 Query: 123 ATVKYAIPYL-KANGGSILINSSINGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYK 181 + +A+P + K GGSI+ +SI G + + G AYS+ KA + AA EL+ K Sbjct: 119 MGMTHALPLMQKRGGGSIINTASIAGLQ--AGFGPLAYSSAKAAVIHMSRCAAAELSPQK 176 Query: 182 IRVNAICPGAITTNIDDNT--YPSE--DLKEVQIAVEFPDGGQPLEKGPGRPDQVARLAL 237 IRVNAICPG I T+I + P E D QIA P QP+ K G P+ +A AL Sbjct: 177 IRVNAICPGLIATSIFGASMGLPREVADQMAAQIASIGPK-IQPIPKS-GLPEDIAAAAL 234 Query: 238 FLASDDSDHITGTEIYCDG 256 +LASDDS +TGT I DG Sbjct: 235 YLASDDSRFVTGTHIVVDG 253 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 115 bits (288), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 0/233 (0%), Positives = 0/233 (0%), Gaps = 13/233 (5%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 + + A + Sbjct: 23 TNGHNVVIYRNHIPAQTLIDRLATMKNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIG 82 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYL-KANGGSILINSS 144 + + + + +I G A P + N Sbjct: 83 ICLGHQAIVEAYGGYVGQ-AGEILHGKASSIEHDGQAMFAGLANPLPVARYHSLVGSNVP 141 Query: 145 INGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSE 204 + + + + + + Sbjct: 142 AGL--TINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLE--PT 197 Query: 205 DLKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGA 257 Sbjct: 198 ------- 243 >STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase Length = 334 Score = 115 bits (288), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 14/233 (6%), Positives = 37/233 (15%), Gaps = 13/233 (5%) Query: 26 LAFARSGAKVAMLDRTVENAEKVRKQXXXXXXXXXXIECDIAEPQQVENAVKQAAGQWGR 85 F+ ++ ++ + + A G Sbjct: 23 QLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPEYLFADIVGT 82 Query: 86 LDIVFANAGINGAMTPIETMDIESWDQTMNINLRGTFATVKYAIPYL-KANGGSILINSS 144 + I+ A + + + + + + Sbjct: 83 GGDGSNSINISTASAFVA-AACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNADKSRQ 141 Query: 145 INGNRVFSNIGFSAYSTTKAGQTAFMKMAALELAQYKIRVNAICPGAITTNIDDNTYPSE 204 A + A L+ + + A Sbjct: 142 ALD--ELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV--YS 197 Query: 205 DLKEVQIAVEFPDGGQPLEKGPGRPDQVARLALFLASDDSDHITGTEIYCDGA 257 + IA E + V E++ Sbjct: 198 PELVLPIA-ETLRVLG------YQRAAVVHSGGMDEVSLHAPTIVAELHDGEI 243 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,039,329 Number of extensions: 591387 Number of successful extensions: 1823 Number of sequences better than 1.0e-05: 276 Number of HSP's gapped: 1437 Number of HSP's successfully gapped: 276 Length of query: 263 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 159 Effective length of database: 26,383,695 Effective search space: 4195007505 Effective search space used: 4195007505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)