BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0009.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3225 [S] COG1285 Uncharacterized membrane protein 291 8e-79 BH0541 [S] COG1285 Uncharacterized membrane protein 223 2e-58 YPO1660 [S] COG1285 Uncharacterized membrane protein 96 4e-20 YPO2332 [S] COG1285 Uncharacterized membrane protein 92 7e-19 PA4635 [S] COG1285 Uncharacterized membrane protein 84 4e-16 >BH3225 [S] COG1285 Uncharacterized membrane protein Length = 232 Score = 291 bits (745), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 137/234 (58%), Positives = 184/234 (78%), Gaps = 5/234 (2%) Query: 55 ESIIKLLVAMLFGLFIGIDRQLKQKPLGIRTSMVISIASCLVTLVSIHAYDKFGGPDHPN 114 E ++KL +A+ FGL IGIDRQLK KPLG++TSMVI +ASCLVT+VS+ ++ +F P N Sbjct: 3 ELLMKLSIALFFGLLIGIDRQLKHKPLGLKTSMVICVASCLVTIVSVESFHRFATPMFNN 62 Query: 115 MDPMRLAAQIVSGIGFLGAGVILRRGGDAISGLTSAALIWTASGIGIAVGAGFYVEAGYA 174 MDPMRLAAQIVSG+GFLGAGVILR+ D ISGLT+AA+IW ASG+GIAVGAGFY+EAGY Sbjct: 63 MDPMRLAAQIVSGVGFLGAGVILRKRNDVISGLTTAAMIWAASGLGIAVGAGFYIEAGYM 122 Query: 175 VVLLMFAVNAVPHLIKLIGPEVLNKHEISVKIIMEHNYVLTDVIQKVEERHVVKTRKTKK 234 VVLLM A+N P L+K +GP L + +++VK++ME N +T++IQ +E KT KK Sbjct: 123 VVLLMLAINVFPQLVKKVGPSSLRQRDVAVKMVMEPNMRMTELIQSIER----KTSMAKK 178 Query: 235 NGRT-IRRMKIKDLEDGRQMIDMVISAPDREYATEIYYDVKKIEHVISVEVEQL 287 IR +K+KDLE+G Q +++++SAP+++Y TEIYY +KKI+HV++V+VE L Sbjct: 179 GSDIRIRHLKLKDLENGNQQVELILSAPEKQYTTEIYYLIKKIDHVLTVDVEHL 232 >BH0541 [S] COG1285 Uncharacterized membrane protein Length = 239 Score = 223 bits (569), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 117/236 (49%), Positives = 165/236 (69%), Gaps = 3/236 (1%) Query: 55 ESIIKLLVAMLFGLFIGIDRQLKQKPLGIRTSMVISIASCLVTLVSIHAYDKFGGPDHP- 113 E +IKL ++++ GL IGIDRQ+K KPLG+RTSMVI +ASCL+T+VSI + G H Sbjct: 4 ELLIKLSLSLVLGLLIGIDRQMKHKPLGLRTSMVICVASCLLTIVSIEHISRSGLIFHNT 63 Query: 114 -NMDPMRLAAQIVSGIGFLGAGVILRRGGDAISGLTSAALIWTASGIGIAVGAGFYVEAG 172 N+DPMRLAAQIVSGIGFLGAGVILRR D ISGLT+AA+IW ASG+GIA+GAGFY E+ Sbjct: 64 NNVDPMRLAAQIVSGIGFLGAGVILRRNNDVISGLTTAAMIWAASGLGIAIGAGFYFESF 123 Query: 173 YAVVLLMFAVNAVPHLIKLIGPEVLNKHEISVKIIMEHNYVLTDVIQKVEE-RHVVKTRK 231 +AV+L++ AVN +P + + +GP L ++ VK+++ + +VI+ + + KT + Sbjct: 124 FAVILILIAVNVIPIMFRWVGPASLRHRDVGVKMVLTEQANIANVIEAINQTSDATKTFR 183 Query: 232 TKKNGRTIRRMKIKDLEDGRQMIDMVISAPDREYATEIYYDVKKIEHVISVEVEQL 287 +K+ IR +K+KDLE +Q + +S P +Y+ E +Y +K I V SVE+ L Sbjct: 184 KRKDRVDIRNLKMKDLEANKQQLQFTLSIPSHQYSVEFFYKLKAIPSVDSVELGDL 239 >YPO1660 [S] COG1285 Uncharacterized membrane protein Length = 236 Score = 96.3 bits (238), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 52/125 (41%), Positives = 77/125 (61%), Gaps = 10/125 (8%) Query: 57 IIKLLVAMLFGLFIGIDRQLKQKPLGIRTSMVISIASCLVTLVSIHAYDKFGGPDHPNMD 116 ++ LL+AM G IG +RQ +Q+ G+RT+ +++ + + L S PD P Sbjct: 11 VMNLLLAMCLGALIGAERQWRQRMAGLRTNALVATGAAVFILSSYAT-----SPDSPG-- 63 Query: 117 PMRLAAQIVSGIGFLGAGVILRRGGDAISGLTSAALIWTASGIGIAVGAGFYVEAGYAVV 176 R+AAQIVSGIGFLGAGVI+R G + I GL +AA +W ++GIG+ G G Y A +A Sbjct: 64 --RIAAQIVSGIGFLGAGVIMREGMN-IRGLNTAATLWCSAGIGVLCGLGLYWNAAFATA 120 Query: 177 LLMFA 181 +++ A Sbjct: 121 VILCA 125 >YPO2332 [S] COG1285 Uncharacterized membrane protein Length = 219 Score = 92.4 bits (228), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 57/229 (24%), Positives = 121/229 (52%), Gaps = 15/229 (6%) Query: 57 IIKLLVAMLFGLFIGIDRQLKQKPLGIRTSMVISIASCLVTLVSIHAYDKFGGPDHPNMD 116 ++++ +A + G IG++RQL+ K G+RT +++ I S + +VS + ++ +H + D Sbjct: 6 LLRITLAGILGGLIGLERQLRAKEAGLRTHILVGIGSAMFMIVSKYGFEDLLVLEHVSFD 65 Query: 117 PMRLAAQIVSGIGFLGAGVILRRGGDAISGLTSAALIWTASGIGIAVGAGFYVEAGYAVV 176 P R+AAQ+VSG+GFLGAG I+ + + GLT+AA +W + IG+ +G+G Y Y Sbjct: 66 PSRVAAQVVSGMGFLGAGTIMIQ-KQMVKGLTTAAGMWVTAAIGLVIGSGLYEIGIYGT- 123 Query: 177 LLMFAVNAVPHLIKLIGPEVLNKHEISVKIIMEHNYVLTDVIQKVEERHVVKTRKTKKNG 236 L+ L+ EV ++S +++ +H Y++ ++ + +V +K Sbjct: 124 -----------LMTLVVLEVFR--QLSQRLLGQHYYIVVKLLPESVPLVLVALQKLHLQP 170 Query: 237 RTIRRMKIKDLEDGRQMIDMVISAPDREYATEIYYDVKKIEHVISVEVE 285 + + ++ + M ++ ++ ++Y + IE + ++++ Sbjct: 171 MNLSVTQNHKSDNTSSELTMEVTLSPKKTVNQVYEQLLAIEGIHQLDIK 219 >PA4635 [S] COG1285 Uncharacterized membrane protein Length = 234 Score = 83.6 bits (205), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 47/134 (35%), Positives = 79/134 (58%), Gaps = 11/134 (8%) Query: 48 MDFNLHMESIIKLLVAMLFGLFIGIDRQLKQKPLGIRTSMVISIASCLVTLVSIHAYDKF 107 MD+ + + +++ VA++ G IG +RQL+Q+ G+RT+ ++S +CL L++ Sbjct: 1 MDWKVFL---LRVAVALVLGALIGAERQLRQRLTGLRTNALVSTGACLFVLMT-QGVPGL 56 Query: 108 GGPDHPNMDPMRLAAQIVSGIGFLGAGVILRRGGDAISGLTSAALIWTASGIGIAVGAGF 167 G D R+AA +VSGIGFLG GVI+R G + + GL +AA +W + IG+ G Sbjct: 57 AG------DASRIAAYVVSGIGFLGGGVIMRDGLN-VRGLNTAATLWCTAAIGVLCSMGL 109 Query: 168 YVEAGYAVVLLMFA 181 +EA ++++ A Sbjct: 110 LLEATLGSLVVLCA 123 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,011,241 Number of extensions: 885232 Number of successful extensions: 4468 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 4452 Number of HSP's successfully gapped: 11 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)