BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0032.p (510 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_pksL_1 [Q] COG3321 Polyketide synthase modules and related pr... 303 5e-82 BS_pksK_2 [Q] COG3321 Polyketide synthase modules and related pr... 282 1e-75 BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related pr... 280 6e-75 BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related pr... 274 2e-73 all2635 [Q] COG3321 Polyketide synthase modules and related prot... 259 9e-69 >BS_pksL_1 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1814 Score = 303 bits (775), Expect = 5e-82, Method: Compositional matrix adjust. Identities = 182/501 (36%), Positives = 277/501 (55%), Gaps = 36/501 (7%) Query: 7 SSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNSIGITA 66 S+D +TF +G EG GAVLLKPL++A +D D I+AVIKGSA+N G GI+ Sbjct: 544 STDGHCRTFGKDGDGYVPAEGIGAVLLKPLRQAEEDGDRIYAVIKGSAVNHVGTVSGISV 603 Query: 67 PDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKKQFCA 126 P P +Q +++ E+ GI+P +SY+EAHGTGT LGDPIEI+G+ +AF +YTQ +QFC+ Sbjct: 604 PSPVSQADLIETCLEKTGIDPRTISYVEAHGTGTSLGDPIEIQGLVKAFRQYTQDRQFCS 663 Query: 127 IGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVNDRLQE 186 IGS+K+NIGH + AAGI+GL KV L +KL PSLH + N ++F S YV + Sbjct: 664 IGSVKSNIGHAESAAGISGLSKVALQLHHQKLVPSLHSEELNPYVDFEKSPFYVQHETET 723 Query: 187 WTTPGV---------PRLAGISSFGLSGTNCHMVLEEAPPPPQKQQ-----HGETMYLLP 232 W P + PR AGISSFG +G+N H++LEE P +Q + ++P Sbjct: 724 WKQPVIKENGEDVPYPRRAGISSFGATGSNAHIILEEHIPQAAEQDVSLSSDSDISAVIP 783 Query: 233 LSAKEENVLRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQL 292 LSA+ + LR A + L QI DL YT GR R++ + +G Q+L +QL Sbjct: 784 LSARNQERLRVYAKRLLDFLHDGIQI--RDLAYTLQVGREPMEERVSFLASGIQELSDQL 841 Query: 293 ERLTEKQALTPTLQSAQQLKGMEHSEKENSYRA---------IAELYVSGGTIDWEDMYK 343 + E + + +G E S S IAE + +G +DW+ +Y+ Sbjct: 842 KAFIEGRKAIQHCWKGRVSRGSEPSRPAESVHKLLEQRKLDQIAEQWANGSGVDWKLLYE 901 Query: 344 GKACNRLSLPVYPFQKTRCWLKPEAQAEPKRSASGGNV-HPLLAHVAGETPEQVTYAVRM 402 G R+SLP YPF++ R W+ P+A+ + RS ++ HPLL H V + Sbjct: 902 GSKPKRISLPTYPFERVRYWV-PKAEKKTDRSKQERHILHPLL-HQNVSDISGVRFRSAF 959 Query: 403 SAKEDWMLNEHKVGSEYVLPGTAYVELLLEIGRQQGGRSV-----IKDLAFLQPFSVQEG 457 + +E + L +H + E+VLPG A +E++ + ++++ +++PF+V E Sbjct: 960 TGRE-FFLKDHVIKGEHVLPGAALLEMVRAAVERAAADQFPTGFRLRNIVWVRPFAVTE- 1017 Query: 458 ETRELQITL-KKGNGDNGFII 477 + +++ + L + NG+ F I Sbjct: 1018 QQKDIDVRLYPEENGEITFEI 1038 >BS_pksK_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1575 Score = 282 bits (721), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 186/541 (34%), Positives = 282/541 (52%), Gaps = 49/541 (9%) Query: 7 SSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDG-NSIGIT 65 SSD K FD S++G GEG VLLK A++D DHI+A+++G +N DG + +G Sbjct: 217 SSDGHIKAFDASADGMIGGEGVAVVLLKKAADAVKDGDHIYALLRGIGVNNDGADKVGFY 276 Query: 66 APDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKKQFC 125 AP + Q +V+ Q + + PE++ Y+EAHGTGT+LGDPIE+ + + +YT K QFC Sbjct: 277 APSVKGQADVVQQVMNQTKVQPESICYVEAHGTGTKLGDPIELAALTNVYRQYTNKTQFC 336 Query: 126 AIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVNDRLQ 185 IGS+KTNIGHLD AAG+AG +KV+ SL ++L PS+++ +PN + ++S YV D+ + Sbjct: 337 GIGSVKTNIGHLDTAAGLAGCIKVVMSLYHQELAPSVNYKEPNPNTDLASSPFYVVDQKK 396 Query: 186 EWTTPGVPRLAGISSFGLSGTNCHMVLEEAPPPPQKQQHGETMYLLPLSAKEENVLRELA 245 + A +SSFGL GTN H + E+ K + T ++P+SAK + L+E A Sbjct: 397 TLSREIKTHRAALSSFGLGGTNTHAIFEQFKRDSDKGKIDGTC-IVPISAKNKERLQEYA 455 Query: 246 AAYIRLLE--GSSQIDLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQL-ERLTEKQALT 302 + LE G L D YT GR HR+ + D +N+L +RLT+ Sbjct: 456 EDILAYLERRGFENSQLPDFAYTLQVGREAMEHRVVFI----ADHVNELKQRLTDFINGN 511 Query: 303 PTLQSAQQLKGMEHSEKENSY--------------------RAIAELYVSGGTIDWEDMY 342 ++ +G +H+ +E S+ +AE++ G IDW +Y Sbjct: 512 TAIEGC--FQGSKHNAREVSWLTEDEDSAELIRKWMAKGKVNKLAEMWSKGAHIDWMQLY 569 Query: 343 KGKACNRLSLPVYPFQKTRCW-----LKPEAQAEPKRSASGGNVHPLLAHVAGETPEQVT 397 KG+ NR+SLP YPF K R W KP AQ ++ G++HPLL + EQ Sbjct: 570 KGERPNRMSLPTYPFAKERYWPSQDDRKPVAQISGNQTGI-GSIHPLLHQNTSDFSEQKF 628 Query: 398 YAVRMSAKEDWMLNEHKVGSEYVLPGTAYVELLLEIGRQQGGRSVIKDL-------AFLQ 450 +V +++ L +H V + VLPG AY+E+ Q G + +D+ ++Q Sbjct: 629 SSVFTG--DEFFLRDHVVRGKPVLPGVAYLEMAYAAINQAAGSEIGQDVRIRLNHTVWVQ 686 Query: 451 PFSVQEGETRELQITL-KKGNGDNGFIITSKDYADGTWRRHAEGKLCLPSQARKPY-DLR 508 P V + + ++ I+L + +G F I S H++G L S A P DL Sbjct: 687 PVVV-DRHSAQVDISLFPEEDGKITFDIYSTQEDGDDPVIHSQGSAELASAAETPVADLT 745 Query: 509 E 509 E Sbjct: 746 E 746 >BS_pksL_3 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1206 Score = 280 bits (715), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 164/395 (41%), Positives = 229/395 (57%), Gaps = 23/395 (5%) Query: 4 GIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNSIG 63 G+ S D + K FD+ +NG GEGAGA++LK LK A DRDHI+ +I GS INQDG + G Sbjct: 208 GMLSPDGQCKAFDNGANGFVPGEGAGALVLKRLKDAEADRDHIYGIIIGSGINQDGKTNG 267 Query: 64 ITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKKQ 123 ITAP ++Q ++ +E GI+PE++SY+E HGTGT+ GDPIE+E + F T KKQ Sbjct: 268 ITAPSAKSQMDLERDIYETYGIHPESISYVEMHGTGTKQGDPIELEALSTVFQEKTDKKQ 327 Query: 124 FCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVNDR 183 FCAIGS+K+NIGH AAG+AG+ KV+ + K L P+L+F PN+ F S +YVN Sbjct: 328 FCAIGSVKSNIGHTSAAAGVAGVQKVLLCMNHKTLVPTLNFTTPNEHFEFEHSPLYVNTE 387 Query: 184 LQEW-TTPGVPRLAGISSFGLSGTNCHMVLEEAPPPPQK----QQHGETMYLLPLSAKEE 238 L+ W T G PR A +SSFG SGTN H+V+EE P + +QH +++ LSAK+E Sbjct: 388 LKPWETADGKPRRACVSSFGYSGTNAHIVIEEYQPEKRNDRLTKQHRSALFV--LSAKKE 445 Query: 239 NVLRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLA-------IVFTGYQDLLNQ 291 L+ A A + + IDLED+ YT TGR ++R+A ++ D L + Sbjct: 446 KQLKAYAEAMKDFVTSNEDIDLEDMAYTLQTGREAMDYRMAFLADSREMLIKALDDYLAE 505 Query: 292 LERLTEKQALTPTLQSAQQLKGMEHSEK--------ENSYRAIAELYVSGGTIDWEDMYK 343 + + A T +S +L +H K + +AEL+V G IDW +Y Sbjct: 506 MPNASIFAAHVKTKKSEIKLFETDHDAKALLQTWIEKKRLEKVAELWVKGLQIDWNKLYG 565 Query: 344 GKACNRLSLPVYPFQKTRCWLKPEAQAEPKRSASG 378 R+SLP YPF + WL P + EP+ A+ Sbjct: 566 EYTPRRISLPAYPFAEEYYWL-PTQEGEPETIATA 599 >BS_pksL_2 [Q] COG3321 Polyketide synthase modules and related proteins Length = 211 Score = 274 bits (701), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 0/398 (0%), Positives = 0/398 (0%), Gaps = 29/398 (7%) Query: 7 SSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNSIGITA 66 Sbjct: 224 283 Query: 67 PDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKKQFCA 126 Sbjct: 284 343 Query: 127 IGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVNDRLQE 186 Sbjct: 344 403 Query: 187 WTTPGV---------PRLAGISSFGLSGTNCHMVLEEAPPPPQKQQ--HGETMYLLPLSA 235 Sbjct: 404 463 Query: 236 KEENVLRELAAAYIRLLEGSSQI--DLEDLCYTASTGRRHYNHRLAIVFTGYQDLLNQLE 293 Sbjct: 464 523 Query: 294 RL----------------TEKQALTPTLQSAQQLKGMEHSEKENSYRAIAELYVSGGTID 337 Sbjct: 524 583 Query: 338 WEDMYKGKACNRLSLPVYPFQKTRCWLKPEAQAEPKRS 375 Sbjct: 584 621 >all2635 [Q] COG3321 Polyketide synthase modules and related proteins Length = 1565 Score = 259 bits (661), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 133/281 (47%), Positives = 179/281 (63%), Gaps = 3/281 (1%) Query: 4 GIESSDEKTKTFDDSSNGTGKGEGAGAVLLKPLKKALQDRDHIHAVIKGSAINQDGNS-I 62 G+ S D + FD ++ GT G G G V+LK L+ A+QD DHI+A+IK +AIN DG+ + Sbjct: 251 GVLSPDGYCRAFDVNAKGTVFGNGVGIVVLKRLRDAIQDGDHIYAIIKATAINNDGSQKV 310 Query: 63 GITAPDPQAQEEVLCQAWEEAGINPENLSYIEAHGTGTRLGDPIEIEGIDRAFSRYTQKK 122 G+TAP Q + + A +A INPE + YIE HGTGT LGDPIEI + +AFS +T +K Sbjct: 311 GLTAPSVTGQAKAITTALTKAKINPETIQYIETHGTGTALGDPIEIAALTKAFSPHTHQK 370 Query: 123 QFCAIGSMKTNIGHLDHAAGIAGLVKVIYSLKSKKLFPSLHFMKPNQTINFSTSAVYVND 182 QFCA+GS+KTNIGHLD AAGI+GL+K +LK K++ PSL+F N I+F+ S +VN Sbjct: 371 QFCAVGSVKTNIGHLDAAAGISGLIKTALALKHKQIPPSLNFTAANPQIDFANSPFFVNT 430 Query: 183 RLQEWTTPGVPRLAGISSFGLSGTNCHMVLEEAPP--PPQKQQHGETMYLLPLSAKEENV 240 +L W PR AG+SSFG+ GTN H +LEEAP Q + +LL LSAK E Sbjct: 431 QLTPWINHQHPRRAGVSSFGMGGTNAHAILEEAPTQFKIQNSKFKINYHLLCLSAKSEKA 490 Query: 241 LRELAAAYIRLLEGSSQIDLEDLCYTASTGRRHYNHRLAIV 281 L + L+ +++L D+ YT GRR +NHR +V Sbjct: 491 LEQATINLATHLQKHPELNLADVAYTLQIGRRAFNHRRCLV 531 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,468,427 Number of extensions: 1713466 Number of successful extensions: 2274 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 2140 Number of HSP's successfully gapped: 102 Length of query: 510 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 399 Effective length of database: 25,576,357 Effective search space: 10204966443 Effective search space used: 10204966443 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)