BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0027.p (882 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0641_5 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 479 e-134 PA2399_1 [Q] COG1020 Non-ribosomal peptide synthetase modules an... 419 e-116 PA2399_2 [Q] COG1020 Non-ribosomal peptide synthetase modules an... 417 e-116 RSp0641_7 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 411 e-114 RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules a... 405 e-112 >RSp0641_5 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 479 bits (1232), Expect = e-134, Method: Compositional matrix adjust. Identities = 0/865 (0%), Positives = 0/865 (0%), Gaps = 37/865 (4%) Query: 13 NDVYKKEQQYWLHKLAGLP---DKSHFPCEPHKDRHQERHIETIPFSLDPVHARKLVQIS 69 V + H L + + + E ++ + L + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQY---EGVELLAYSCLGFGT 240 Query: 70 NDSDARLHVLLLAFTSVWLLKYSGQTDIVLGTSIY---RQPSEEDFINTVL---PVRVQV 123 + +++ + ++ + VR Q Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 124 QNEMRLIDLISLMKQTVSEAARHQNYPFELMLKQAGL-DSGFLDVSVIVSNIQ------- 175 L+ ++ + + + H +++ + + Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVH 360 Query: 176 --------DEQYLSGTLSNFTMIVHREESVLQGEIRFNAAVYSADTVNQLLEHFHVLLEQ 227 Sbjct: 361 420 Query: 228 SLADLHSSVQELSILTEKDRRIIACSNATSAGFPEEA-TIPALFEECAAKYPNHTAAEFA 286 Sbjct: 421 480 Query: 287 DERLSYQDLNRKANQAAHHLRRLGVTAESVVALILPPSLEMIIGILAVLKAGGAYLPIDP 346 Sbjct: 481 540 Query: 347 DLPAQRVDYILKDSQAGQILTVQSLSLPPGTGESCEVIFLDDPMLEQYSSSNLEHVIHPH 406 Sbjct: 541 - 599 Query: 407 DLAYIIYTSGTTGNPKGVMIEHRNVVRLFFHEHNRFDFTCQDTWTLFHSFGFDFSVWEIF 466 Sbjct: 600 659 Query: 467 XXXXXXXXXXXXXXXXXRDYRHIVQELRNEHVTVFNQTPSSFYQFMQEELEDTGEKLRLR 526 Sbjct: 660 719 Query: 527 YVILGGEALQPGKLEAW--KVKYPGTRIINMYGITETTVHTTFKEIGTEQIQANQNNIGV 584 Sbjct: 720 779 Query: 585 PFSTVEVYIFNRQHEQVPIXXXXXXXXXXXXXXXXYLNRTELTEDRFQTRRM-GDRWIRL 643 Sbjct: 780 839 Query: 644 YRTGDLGRYLPSGELEYVGRRDNQVKIRGFRIELDEISAHLLTVESIKEAVVSAREIEPN 703 Sbjct: 840 899 Query: 704 AQILVAYVVIKEGQEAAFDPVEIRQYLQTRISDYMIPSVLLPLSKIPLTLNGKVDYEKLD 763 Sbjct: 900 ---- 955 Query: 764 TLELKQQAKGVLTPPTTEMEHRIAEIWKTSLRMDRIGVHSTIFDLGGTSFDVITISKALS 823 Sbjct: 956 1015 Query: 824 EELGQEVPVITLFTYPTISMLAKVL 848 Sbjct: 1016 1040 >PA2399_1 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 419 bits (1077), Expect = e-116, Method: Compositional matrix adjust. Identities = 0/875 (0%), Positives = 0/875 (0%), Gaps = 51/875 (5%) Query: 17 KKEQQ--YWLHKLAGLPDKSHFPCE-PHKDRHQERHIETIPFSLDPVHARKLVQISNDSD 73 + + Y L K + A Sbjct: 226 EGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRY-SGEQTQKAVAAYVDIARRH 284 Query: 74 ARLHVLLLAFTSVWLLKYSGQTDIVLGTSIYRQPSEED------FINTVLPVRVQVQNEM 127 + + + E + + V Sbjct: 285 GLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI-EAVHQVYTY 343 Query: 128 RLIDLISLMKQTVSEAARHQNYPFELMLKQAGLDSGFLDVSV--IVSNIQDEQYLSGTLS 185 Sbjct: 344 403 Query: 186 NF-------------------TMIVHREESVLQGEIRFNAAVYSADTVNQLLEHFHVLLE 226 Sbjct: 404 463 Query: 227 QSLADLHSSVQELSILTEKDRR-IIACSNATSAGFPEEATIPALFEECAAKYPNHTAAEF 285 Sbjct: 464 523 Query: 286 ADERLSYQDLNRKANQAAHHLRRLGVTAESVVALILPPSLEMIIGILAVLKAGGAYLPID 345 Sbjct: 524 583 Query: 346 PDLPAQRVDYILKDSQAGQILTVQSL--SLPPGTGESCEVIFLDDPMLEQYSSSNLEHVI 403 Sbjct: 584 -- 641 Query: 404 HPHDLAYIIYTSGTTGNPKGVMIEHRNVVRLFFHEHNRFDFTCQDTWTLFHSFGFDFSVW 463 Sbjct: 642 701 Query: 464 EIFXXXXXXXXXXXXXXXXXRDYRHIVQELRNEHVTVFNQTPSSFYQFMQEEL--EDTGE 521 Sbjct: 702 761 Query: 522 KLRLRYVILGGEALQPGKLEAWKVKYPGTR---IINMYGITETTVHTTFKEIGTEQIQAN 578 Sbjct: 762 - 820 Query: 579 Q-NNIGVPFSTVEVYIFNRQHEQVPIXXXXXXXXXXXXXXXXYLNRTELTEDRFQTRRM- 636 Sbjct: 821 880 Query: 637 GDRWIRLYRTGDLGRYLPSGELEYVGRRDNQVKIRGFRIELDEISAHLLTVESIKEAVVS 696 Sbjct: 881 940 Query: 697 AREIEPNAQILVAYVVIKEGQEAAFDPVEIRQYLQTRISDYMIPSVLLPLSKIPLTLNGK 756 Sbjct: 941 - 999 Query: 757 VDYEKLDTLE--LKQQAKGVLTPPTTEMEHRIAEIWKTSLRMDRIGVHSTIFDLGGTSFD 814 Sbjct: 1000 --- 1056 Query: 815 VITISKALSEELGQEVPVITLFTYPTISMLAKVLQ 849 Sbjct: 1057 1091 >PA2399_2 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 417 bits (1072), Expect = e-116, Method: Compositional matrix adjust. Identities = 0/877 (0%), Positives = 0/877 (0%), Gaps = 49/877 (5%) Query: 17 KKEQQ--YWLHKLAGLPDKSHFPCEPHKDRHQERHIETIPFSLDPVHARKLVQISNDSDA 74 Y + P+ ++ + S Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 75 RLHVLLLAFTSVWLLKYSGQTDIVLGTSIYRQPSEED------FINTVLPVRVQVQNEMR 128 L A + + G + + ++ + + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQ-MALAFVRRQP 300 Query: 129 LIDLISLMKQTVSEAARHQNYPFELML------KQAGLDSGFL-------DVSVIVSNIQ 175 + L T+ + + + +A Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVYTY 360 Query: 176 DEQY------LSGTLSNF--TMIVHREESVLQGEIRFNAAVYSADTVNQLLEHFHVLLEQ 227 Sbjct: 361 420 Query: 228 SLADLHSSVQELSILTEKDRR-IIACSNATSAGFPEEATIPALFEECAAKYPNHTAAEFA 286 Sbjct: 421 480 Query: 287 DERLSYQDLNRKANQAAHHLRRLGVTAESVVALILPPSLEMIIGILAVLKAGGAYLPIDP 346 Sbjct: 481 540 Query: 347 DLPAQRVDYILKDSQAGQILTVQSL--SLPPGTGESCEVIFLDDPMLEQYSSSNLEHVIH 404 Sbjct: 541 -- 598 Query: 405 PHDLAYIIYTSGTTGNPKGVMIEHRNVVRLFFHEHNRFDFTCQDTWTLFHSFGFDFSVWE 464 Sbjct: 599 658 Query: 465 IFXXXXXXXXXXXXXXXXXRDYRHIVQELRNEHVTVFNQTPSSFYQFMQEEL--EDTGEK 522 Sbjct: 659 718 Query: 523 LRLRYVILGGEALQPGKLEAWKVKYPGTR---IINMYGITETTVHTTFKEIGTEQIQANQ 579 Sbjct: 719 - 777 Query: 580 -NNIGVPFSTVEVYIFNRQHEQVPIXXXXXXXXXXXXXXXXYLNRTELTEDRFQTRRM-G 637 Sbjct: 778 837 Query: 638 DRWIRLYRTGDLGRYLPSGELEYVGRRDNQVKIRGFRIELDEISAHLLTVESIKEAVVSA 697 Sbjct: 838 897 Query: 698 REIEPNAQILVAYVVIKEGQEAAFDPVEIRQYLQTRISDYMIPSVLLPLSKIPLTLNGKV 757 Sbjct: 898 - 956 Query: 758 DYEKLDTLE--LKQQAKGVLTPPTTEMEHRIAEIWKTSLRMDRIGVHSTIFDLGGTSFDV 815 Sbjct: 957 --- 1013 Query: 816 ITISKALSEELGQEVPVITLFTYPTISMLAKVLQADS 852 Sbjct: 1014 1050 >RSp0641_7 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 0/860 (0%), Positives = 0/860 (0%), Gaps = 39/860 (4%) Query: 17 KKEQQYWLHKLAGLPDKSHFPCEPHKDRHQERHIETIPFSLDPVHARKLVQISNDSDARL 76 ++ L ++ + + + E L + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 77 HVLLLAFTSVWLLKYSGQTDIVLGTSIYRQPSEED------FINTVLPVRVQVQNEMRLI 130 +YSG+ + + + + V + + L Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAF-VRRQPFVASTL-LG 308 Query: 131 DLISLMKQTVSEAARHQNYPFELMLKQ-----------------AGLDSGFLDVSVIVSN 173 +T E+ + L + Sbjct: 309 ATTMDQLKTNIESLHLELSEDVLAEIE 368 Query: 174 IQDEQYLSGTLS---NFTMIVHREESVLQGEIRFNAAVYSADTVNQLLEHFHVLLEQSLA 230 Sbjct: 369 428 Query: 231 DLHSSVQELSILTEKDR-RIIACSNATSAGFPEEATIPALFEECAAKYPNHTAAEFADER 289 Sbjct: 429 488 Query: 290 LSYQDLNRKANQAAHHLRRLGVTAESVVALILPPSLEMIIGILAVLKAGGAYLPIDPDLP 349 Sbjct: 489 548 Query: 350 AQRVDYILKDSQAGQILTVQSLSLPPGTGESCEVIFLDDPMLEQYSSSNLEHVIHPHDLA 409 Sbjct: 549 -- 606 Query: 410 YIIYTSGTTGNPKGVMIEHRNVVRLFFHEHNRFDFTCQDTWTLFHSFGFDFSVWEIFXXX 469 Sbjct: 607 666 Query: 470 XXXXXXXXXXXXXXRDYRHIVQELRNEHVTVFNQTPSSFYQFMQEELEDTGEKLRLRYVI 529 Sbjct: 667 -- 724 Query: 530 LGGEALQPGKLEAWKVKYPGTRIINMYGITETTVHTTFKEIGTEQIQANQNNIGVPFSTV 589 Sbjct: 725 - 783 Query: 590 EVYIFNRQHEQVPIXXXXXXXXXXXXXXXXYLNRTELTEDRFQTRRM-GDRWIRLYRTGD 648 Sbjct: 784 843 Query: 649 LGRYLPSGELEYVGRRDNQVKIRGFRIELDEISAHLLTVESIKEAVVSAREIEPNAQILV 708 Sbjct: 844 903 Query: 709 AYVVIKEGQEAAFDPVEIRQYLQTRISDYMIPSVLLPLSKIPLTLNGKVDYEKLDTLELK 768 Sbjct: 904 ---- 959 Query: 769 QQAKGVLTPPTTEMEHRIAEIWKTSLRMDRIGVHSTIFDLGGTSFDVITISKALSEELGQ 828 Sbjct: 960 1019 Query: 829 EVPVITLFTYPTISMLAKVL 848 Sbjct: 1020 1039 >RSp0641_6 [Q] COG1020 Non-ribosomal peptide synthetase modules and related proteins Length = 346 Score = 405 bits (1042), Expect = e-112, Method: Compositional matrix adjust. Identities = 0/866 (0%), Positives = 0/866 (0%), Gaps = 62/866 (7%) Query: 17 KKEQQYWLHKLAGLPDKSHFPCEPHKDRHQERHIETIPFSLDPVHARKLVQISNDSDARL 76 ++ L ++ + + + E L + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 77 HVLLLAFTSVWLLKYSGQTDIVLGTSIYRQPSEED------FINTVLPVRVQVQNEMRLI 130 +YSG+ + + + + V L Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAF-VRRQPFV-ASTLLG 308 Query: 131 DLISLMKQTVSEAARHQNYPFELMLKQ-----------------AGLDSGFLDVSVIVSN 173 +T E+ + L + Sbjct: 309 ATTMDQLKTNIESLHLELSEDVLAEIE 368 Query: 174 IQDEQYLSGTLS-NFTMIVHREESVLQGEIRFNAAVYSADTVNQLLEHFHVLLEQSLADL 232 Sbjct: 369 428 Query: 233 HSSVQELSILTEKDR-RIIACSNATSAGFPEEATIPALFEECAAKYPNHTAAEFADERLS 291 Sbjct: 429 - 487 Query: 292 YQDLNRKANQAAHHLRRLGVTAESVVALILPPSLEMIIGILAVLKAGGAYLPIDPDLPAQ 351 Sbjct: 488 547 Query: 352 RVDYILKDSQAGQILTVQSLS--LPPGTGESCEVIFLDD--PMLEQYSSSNLEHVIHPHD 407 Sbjct: 548 --- 604 Query: 408 LAYIIYTSGTTGNPKGVMIEHRNVVRLFFHEHNRFDFTCQDTWTLFHSFGFDFSVWEI-- 465 Sbjct: 605 664 Query: 466 -FXXXXXXXXXXXXXXXXXRDYRHIVQELRNEHVTVFNQTPSSFYQFMQEELEDTGEKLR 524 Sbjct: 665 ----- 719 Query: 525 LRYVILGGEALQPGKLEAWKVKYPGTRIINMYGITETTVHTTFKEIGTEQIQANQNNIGV 584 Sbjct: 720 ---------- 769 Query: 585 PFSTVEVYIFNRQHEQVPIXXXXXXXXXXXXXXXXYLNRTELTEDRFQTR--RMGDRWIR 642 Sbjct: 770 --- 826 Query: 643 LYRTGDLGRYLPSGELEYVGRRDNQVKIRGFRIELDEISAHLLTVESIKEAVVSAREIEP 702 Sbjct: 827 886 Query: 703 NAQILVAYVVIKEGQEAAFDPVEIRQYLQTRISDYMIPSVLLPLSKIPLTLNGKVDYEKL 762 Sbjct: 887 ---- 942 Query: 763 DTLELKQQAKGVLTPPTTEMEHRIAEIWKTSLRMDRIGVHSTIFDLGGTSFDVITISKAL 822 Sbjct: 943 1002 Query: 823 SEELGQEVPVITLFTYPTISMLAKVL 848 Sbjct: 1003 1028 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 60,331,036 Number of extensions: 2527043 Number of successful extensions: 5671 Number of sequences better than 1.0e-05: 169 Number of HSP's gapped: 5345 Number of HSP's successfully gapped: 205 Length of query: 882 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 767 Effective length of database: 25,115,021 Effective search space: 19263221107 Effective search space used: 19263221107 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)